{"title":"反向遗传学研究否定了非洲猪瘟病毒基因I196L在猪发病中的作用。","authors":"Ayumi Yoshimoto, Tomoya Kitamura, Mitsutaka Ikezawa, Takehiro Kokuho, Kentaro Masujin","doi":"10.1292/jvms.25-0245","DOIUrl":null,"url":null,"abstract":"<p><p>The African swine fever virus (ASFV) genome encodes over 170 genes, but only a small number have been investigated for their roles in disease progression. Understanding the genetic and biological functions of these lesser-known genes accelerates the specification of open reading frames involved in pathogenesis in host animals and the development of ASF vaccines. In this study, we deleted the I196L gene of the highly virulent parental strain AQS-C-1-22 to generate the ASFV mutant AQSΔI196L. In vitro, AQSΔI196L replicated similarly to the parental virus in immortalized porcine kidney macrophage cultures. Pigs intramuscularly inoculated with 10² median tissue culture infectious dose (TCID<sub>50</sub>) AQSΔI196L developed similar clinical signs and consequences (e.g., high fever, anorexia, and death) as infections with the parental strain. However, viremia in AQSΔI196L-inoculated pigs was slightly reduced compared with pigs inoculated with the parental strain. These findings suggest that the I196L gene does not significantly affect the pathogenicity of the virus in pigs.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Reverse genetics study disclaims the role of African swine fever virus gene I196L in the pathogenesis in pigs.\",\"authors\":\"Ayumi Yoshimoto, Tomoya Kitamura, Mitsutaka Ikezawa, Takehiro Kokuho, Kentaro Masujin\",\"doi\":\"10.1292/jvms.25-0245\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The African swine fever virus (ASFV) genome encodes over 170 genes, but only a small number have been investigated for their roles in disease progression. Understanding the genetic and biological functions of these lesser-known genes accelerates the specification of open reading frames involved in pathogenesis in host animals and the development of ASF vaccines. In this study, we deleted the I196L gene of the highly virulent parental strain AQS-C-1-22 to generate the ASFV mutant AQSΔI196L. In vitro, AQSΔI196L replicated similarly to the parental virus in immortalized porcine kidney macrophage cultures. Pigs intramuscularly inoculated with 10² median tissue culture infectious dose (TCID<sub>50</sub>) AQSΔI196L developed similar clinical signs and consequences (e.g., high fever, anorexia, and death) as infections with the parental strain. However, viremia in AQSΔI196L-inoculated pigs was slightly reduced compared with pigs inoculated with the parental strain. These findings suggest that the I196L gene does not significantly affect the pathogenicity of the virus in pigs.</p>\",\"PeriodicalId\":49959,\"journal\":{\"name\":\"Journal of Veterinary Medical Science\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Medical Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1292/jvms.25-0245\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medical Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1292/jvms.25-0245","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Reverse genetics study disclaims the role of African swine fever virus gene I196L in the pathogenesis in pigs.
The African swine fever virus (ASFV) genome encodes over 170 genes, but only a small number have been investigated for their roles in disease progression. Understanding the genetic and biological functions of these lesser-known genes accelerates the specification of open reading frames involved in pathogenesis in host animals and the development of ASF vaccines. In this study, we deleted the I196L gene of the highly virulent parental strain AQS-C-1-22 to generate the ASFV mutant AQSΔI196L. In vitro, AQSΔI196L replicated similarly to the parental virus in immortalized porcine kidney macrophage cultures. Pigs intramuscularly inoculated with 10² median tissue culture infectious dose (TCID50) AQSΔI196L developed similar clinical signs and consequences (e.g., high fever, anorexia, and death) as infections with the parental strain. However, viremia in AQSΔI196L-inoculated pigs was slightly reduced compared with pigs inoculated with the parental strain. These findings suggest that the I196L gene does not significantly affect the pathogenicity of the virus in pigs.
期刊介绍：
JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.
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