Viruses-Basel最新文献_第3页

A Strand-Specific Quantitative RT-PCR Method for Detecting vRNA, cRNA, and mRNA of H7N9 Avian Influenza Virus in a Mouse Model. 小鼠模型中检测H7N9禽流感病毒vRNA、cRNA和mRNA的链特异性定量RT-PCR方法

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-17 DOI: 10.3390/v17071007

Bo Wang, Guangwen Wang, Yi-Han Wang, Xuwei Liu, Manman Li, Huihui Kong, Hualan Chen, Li Jiang, Chengjun Li

{"title":"A Strand-Specific Quantitative RT-PCR Method for Detecting vRNA, cRNA, and mRNA of H7N9 Avian Influenza Virus in a Mouse Model.","authors":"Bo Wang, Guangwen Wang, Yi-Han Wang, Xuwei Liu, Manman Li, Huihui Kong, Hualan Chen, Li Jiang, Chengjun Li","doi":"10.3390/v17071007","DOIUrl":"https://doi.org/10.3390/v17071007","url":null,"abstract":"Avian influenza virus (AIV) remains a persistent threat to both the poultry industry and human health. Among the AIV subtypes posing public health threats, H7N9 AIV is responsible for five epidemic waves of human infection in China. Here, a detection system based on a mouse model was established, which can simultaneously and quantitatively analyze the dynamic changes in the viral genomic RNA (vRNA), complementary RNA (cRNA), and messenger RNA (mRNA) of H7N9 AIV by using reverse transcription primers with tag sequences to reverse transcribe the three species of RNAs into corresponding cDNA templates, which are then absolutely quantified using the TaqMan quantitative PCR method. This system specifically targets the PB2 and NA genes and, for the first time, enables a spatiotemporal analysis of all three viral RNA species within an animal model. Our results revealed that H7N9 AIV exhibits characteristic replication kinetics, with all three species of viral RNAs showing a rapid increase followed by a certain degree of decline. This system offers a powerful tool for us to further advance our understanding of the replication dynamics of AIV in mice.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Barriers and Facilitators of Implementation of the Non-Hospital-Based Administration of Long-Acting Cabotegravir Plus Rilpivirine in People with HIV: Qualitative Data from the HOLA Study. 在HIV感染者中实施长效卡博特韦加利匹韦林非医院管理的障碍和促进因素：来自HOLA研究的定性数据。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-16 DOI: 10.3390/v17070993

Diana Hernández-Sánchez, Juan M Leyva-Moral, Julian Olalla, Eugènia Negredo, On Behalf Of The Hola Study Group

{"title":"Barriers and Facilitators of Implementation of the Non-Hospital-Based Administration of Long-Acting Cabotegravir Plus Rilpivirine in People with HIV: Qualitative Data from the HOLA Study.","authors":"Diana Hernández-Sánchez, Juan M Leyva-Moral, Julian Olalla, Eugènia Negredo, On Behalf Of The Hola Study Group","doi":"10.3390/v17070993","DOIUrl":"https://doi.org/10.3390/v17070993","url":null,"abstract":"Long-acting (LA) antiretroviral therapies for human immunodeficiency virus (HIV), such as injectable formulations of cabotegravir and rilpivirine (CAB+RPV LA), are now available. Considering the limited data on the out-of-hospital administration of this combination, evaluating the implementation strategies needed is essential to support future clinical efforts. To gather data on barriers and facilitators of implementation for CAB+RPV LA in alternative outpatient facilities, this study used qualitative interviews informed by the Consolidated Framework for Implementation Research (CFIR), with 13 staff participating in the HOLA study (NCT06185452). Data analysis followed qualitative descriptive methods, assisted by Atlas.ti software version 22. The study adhered to the COREQ guidelines. Findings reveal five main factors to consider for implementation: operational and infrastructure adaptations, integrated management of human and organizational resources, need for coordination and follow-up, professional attitudes and work environment, and patient experience and patients' needs perceived by professionals. This study emphasizes the comprehensive operational and infrastructure adaptations, adequate staff training, and supportive professional environment required for the successful implementation of CAB+RPV LA, while considering patients' needs throughout the externalization process (trial registration number: NCT06643897).","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterovirus Detection Trends Based on Respiratory Specimens from a Single Tertiary Hospital in Korea (2018-2024): A Retrospective Study Using Multiplex PCR Data. 基于韩国某三甲医院呼吸道标本的肠道病毒检测趋势（2018-2024）：基于多重PCR数据的回顾性研究

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-16 DOI: 10.3390/v17070991

Jeong Su Han, Sung Hun Jang, Jae-Sik Jeon, Jae Kyung Kim

{"title":"Enterovirus Detection Trends Based on Respiratory Specimens from a Single Tertiary Hospital in Korea (2018-2024): A Retrospective Study Using Multiplex PCR Data.","authors":"Jeong Su Han, Sung Hun Jang, Jae-Sik Jeon, Jae Kyung Kim","doi":"10.3390/v17070991","DOIUrl":"https://doi.org/10.3390/v17070991","url":null,"abstract":"Enteroviruses (EVs) cause broad clinical manifestations, particularly in children. Certain serotypes have been implicated in respiratory infections; however, epidemiological studies analyzing EV circulation based on clinical respiratory specimens are limited in Korea. This retrospective study evaluates EV detection patterns in respiratory specimens to demonstrate their clinical and epidemiological significance as respiratory pathogens in Korea. Respiratory samples collected from outpatient and hospitalized patients with respiratory symptoms at Dankook University Hospital between 2018 and 2024 were analyzed. EV detection patterns were analyzed by year, season, sex, and age. EVs were detected in 303/6292 respiratory specimens. The highest and lowest positivity rates were in 2018 (8.2%) and 2020 (1.6%), likely due to non-pharmaceutical interventions during the COVID-19 pandemic. The highest positivity rates were in summer and autumn, and in children aged 2-11 years and infants aged 0-1 years. EV positivity did not differ significantly between sexes. Significant differences were identified across years, seasons, and age groups. EVs can be detected in respiratory specimens from symptomatic patients and exhibit a marked seasonal distribution and elevated positivity rates in pediatric populations. Hence, EVs may act as atypical respiratory pathogens, underscoring the need for integrated public health surveillance and seasonal prevention strategies.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Wild Side of Science: Rodents and Bats in the Zoonotic Equation. 科学狂野的一面：动物传染病方程式中的啮齿动物和蝙蝠。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-16 DOI: 10.3390/v17070987

Gábor Kemenesi, Kornélia Kurucz

引用次数: 0

Seroprevalence of Equine Influenza Virus Antibodies in Horses from Four Localities in Colombia. 哥伦比亚四个地区马流感病毒抗体的血清流行率。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-16 DOI: 10.3390/v17070999

Juliana Gonzalez-Obando, Jeiczon Jaimes-Dueñez, Angélica Zuluaga-Cabrera, Jorge E Forero, Andrés Diaz, Carlos Rojas-Arbeláez, Julian Ruiz-Saenz

{"title":"Seroprevalence of Equine Influenza Virus Antibodies in Horses from Four Localities in Colombia.","authors":"Juliana Gonzalez-Obando, Jeiczon Jaimes-Dueñez, Angélica Zuluaga-Cabrera, Jorge E Forero, Andrés Diaz, Carlos Rojas-Arbeláez, Julian Ruiz-Saenz","doi":"10.3390/v17070999","DOIUrl":"https://doi.org/10.3390/v17070999","url":null,"abstract":"Equine influenza is a highly contagious disease caused by the equine influenza virus (EIV). The occurrence of EIV outbreaks in America is associated with low levels of vaccination coverage. In Colombia, no seroprevalence evaluation has been carried out to estimate the distribution of the virus within the country. Our aim was to perform a sero-epidemiological survey of equine influenza infections and to identify associated risk factors in horses from four departments of Colombia. Serological testing was carried out by using an ELISA for the detection of IgG antibodies against the influenza A virus. The evaluation of epidemiological variables, clinical manifestations, and vaccination history was carried out through the application of a data collection instrument. Among the 385 horses analyzed, 27% of the samples tested positive, with a higher prevalence in Study 1 from horses with respiratory symptoms (40.4%) than in Study 2 from horses without clinical signs (16.1%). Only horses housed in stables had higher odds of testing positive. The study also revealed that unvaccinated horses were 68% less likely to test positive than vaccinated horses were. This research highlights a significant gap in vaccination coverage and the presence of antibodies even in asymptomatic horses. Management factors such as activity type and housing should be considered when strategies for EIV prevention are developed.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible Crosstalk and Alterations in Gut Bacteriome and Virome in HIV-1 Infection and the Associated Comorbidities Related to Metabolic Disorder. HIV-1感染和代谢紊乱相关合并症中肠道细菌组和病毒组可能的串扰和改变

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-16 DOI: 10.3390/v17070990

Komal Shrivastav, Hesham Nasser, Terumasa Ikeda, Vijay Nema

{"title":"Possible Crosstalk and Alterations in Gut Bacteriome and Virome in HIV-1 Infection and the Associated Comorbidities Related to Metabolic Disorder.","authors":"Komal Shrivastav, Hesham Nasser, Terumasa Ikeda, Vijay Nema","doi":"10.3390/v17070990","DOIUrl":"https://doi.org/10.3390/v17070990","url":null,"abstract":"Improved antiretroviral therapy (ART) has significantly increased the life expectancy of people living with HIV (PLWH). At the same time, other complications like metabolic syndrome (MetS) are coming up as new challenges to handle. This review aims to explore the emerging evidence of gut microbiome and virome alterations in human immunodeficiency virus-1 (HIV-1) infection and associated metabolic disorders, such as type-2 diabetes (T2DM) and cardiovascular disease (CVD), with a focus on their interplay, contribution to immune dysfunction, and potential as therapeutic targets. We conducted a comprehensive review of the current literature on gut bacteriome and virome changes in HIV-1-infected individuals and those with metabolic comorbidities emphasizing their complex interplay and potential as biomarkers or therapeutic targets. HIV-1 infection disrupts gut microbial homeostasis, promoting bacterial translocation, systemic inflammation, and metabolic dysregulation. Similarly, metabolic disorders are marked by reduced beneficial short-chain fatty acid-producing bacteria and an increase in pro-inflammatory taxa. Alterations in the gut virome, particularly involving bacteriophages, may exacerbate bacterial dysbiosis and immune dysfunction. Conversely, some viral populations have been associated with immune restoration post-ART. These findings point toward a dynamic and bidirectional relationship between the gut virome, bacteriome, and host immunity. Targeted interventions such as microbiome modulation and fecal virome transplantation (FVT) offer promising avenues for restoring gut homeostasis and improving long-term outcomes in PLWH.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soluble Neuropilin-1 as a Marker for Distinguishing Bacterial and Viral Sepsis in Critically Ill Patients-A Prospective, Multicenter, Observational Study. 可溶性神经匹林-1作为危重患者区分细菌性和病毒性败血症的标志物——一项前瞻性、多中心、观察性研究

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-16 DOI: 10.3390/v17070997

Fabian Perschinka, Georg Franz Lehner, Timo Mayerhöfer, Frank Hartig, Birgit Zassler, Johannes Bösch, Dietmar Fries, Romuald Bellmann, Michael Joannidis

{"title":"Soluble Neuropilin-1 as a Marker for Distinguishing Bacterial and Viral Sepsis in Critically Ill Patients-A Prospective, Multicenter, Observational Study.","authors":"Fabian Perschinka, Georg Franz Lehner, Timo Mayerhöfer, Frank Hartig, Birgit Zassler, Johannes Bösch, Dietmar Fries, Romuald Bellmann, Michael Joannidis","doi":"10.3390/v17070997","DOIUrl":"https://doi.org/10.3390/v17070997","url":null,"abstract":"Sepsis causes millions of deaths each year. Rapid, targeted therapy can reduce mortality rates. Both bacterial and viral pathogens can trigger sepsis, but the utility of commonly used inflammatory markers for differentiation remains controversial. Moreover, little is known about the time courses of alternative inflammatory parameters. The aim of this prospective, two-center observational study was to investigate the differences in the course of soluble Neuropilin-1 (sNRP-1) levels between bacterial and viral sepsis over a 7-day period. To be included, adult patients had to meet the SEPSIS-3 criteria and be diagnosed with either a bacterial or viral pathogen. Immunosuppressed patients were excluded. While IL-6, PCT, and CRP levels decreased consistently over time, sNRP-1 levels remained elevated in the bacterial group throughout the entire ICU stay. PCT (p < 0.001) and CRP (p = 0.016) levels were significantly associated with the course of sNRP-1. The AUC of sNRP-1 was 0.777 for discriminating between bacterial and viral infections on day 1. sNRP-1 remained stable and significantly higher in bacterial than in viral infections. Furthermore, the AUC values for discrimination ranged from acceptable to good, depending on the day of the ICU stay. sNRP-1 may serve as a potential tool to differentiate between bacterial and viral pathogens in sepsis.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops. 阿根廷大豆作物中四种大豆花叶病毒分离株的生物学、分子和生理学特性

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-16 DOI: 10.3390/v17070995

Mariel Maugeri, Marianela Rodríguez, Nicolas Bejerman, Irma G Laguna, Patricia Rodríguez Pardina

{"title":"Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops.","authors":"Mariel Maugeri, Marianela Rodríguez, Nicolas Bejerman, Irma G Laguna, Patricia Rodríguez Pardina","doi":"10.3390/v17070995","DOIUrl":"https://doi.org/10.3390/v17070995","url":null,"abstract":"Soybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and significant yield losses. In Argentina, during the 1990s, three isolates were collected in Marcos Juárez (MJ), Manfredi (M), and Northwestern Argentina (NOA), along with the \"Planta Vinosa\" (PV) isolate, which causes severe necrosis in some cultivars. These isolates were freeze-dried and stored at -70 °C for several years. They were recovered by mechanical inoculation and biologically, molecularly, and physiologically characterized for the first time. Three of the four isolates showed low genetic divergence in the P1, CI, and CP genes. Although SMV-NOA and SMV-PV had high nucleotide sequence identity, they differed in pathogenicity, seed mottling, and transmission efficiency by seeds or aphids. SMV-NOA caused early changes in photosystem II quantum efficiency (ɸPSII) and malondialdehyde (MDA) content before symptom expression (BS). After symptom development (LS), SMV-M significantly increased MDA, total soluble sugars, and starch compared to the other isolates. Thus, early changes in ɸPSII and sugars may influence late viral symptoms. Likewise, SMV-MJ induced more severe symptoms in the susceptible Davis cultivar than in Don Mario 4800. Therefore, our results demonstrate genomic, biological, and physiological differences among SMV isolates and variable interactions of SMV-MJ with two soybean cultivars.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Cytoplasmic Tail of Ovine Herpesvirus 2 Glycoprotein B Affects Cell Surface Expression and Is Required for Membrane Fusion. 羊疱疹病毒2糖蛋白B的细胞质尾部影响细胞表面表达，是膜融合所必需的。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-16 DOI: 10.3390/v17070994

Colleen M Lynch, Maria K Herndon, McKenna A Hull, Daniela D Moré, Katherine N Baker, Cristina W Cunha, Anthony V Nicola

{"title":"The Cytoplasmic Tail of Ovine Herpesvirus 2 Glycoprotein B Affects Cell Surface Expression and Is Required for Membrane Fusion.","authors":"Colleen M Lynch, Maria K Herndon, McKenna A Hull, Daniela D Moré, Katherine N Baker, Cristina W Cunha, Anthony V Nicola","doi":"10.3390/v17070994","DOIUrl":"https://doi.org/10.3390/v17070994","url":null,"abstract":"Ovine herpesvirus 2 (OvHV-2) causes the fatal veterinary disease malignant catarrhal fever (MCF). Fusion is an essential step in the host cell entry of enveloped viruses and is an important target for vaccine development. OvHV-2 cannot be propagated in vitro, so a robust virus-free cell-cell membrane fusion assay is necessary to elucidate its entry mechanism. OvHV-2 cell-cell fusion requires three conserved herpesviral envelope glycoproteins: gB, gH, and gL. OvHV-2 fusion activity is detectable but low. We hypothesize that enhancing the cell surface expression of gB, which is the core herpesviral fusogen, will increase cell-cell fusion. We generated C-terminal truncation mutants of gB and determined their cell surface expression, subcellular distribution, and fusion activity. Two mutants, including one that lacked the entire cytoplasmic tail domain, failed to function in the cell-cell fusion assay, despite wild-type levels of surface expression. This suggests that the OvHV-2 gB cytoplasmic tail is critical for fusion. A gB mutant truncated at amino acid 847 showed increased surface expression and fusion relative to the wild type. This suggests that the robust fusion activity of gB847 is the result of increased surface expression. gB847 may be used in place of wild-type gB in an improved, more robust OvHV-2 fusion assay.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTED: Branda et al. Zoonotic Paramyxoviruses: Evolution, Ecology, and Public Health Strategies in a Changing World. Viruses 2024, 16, 1688. 撤稿：Branda et al。人畜共患副粘病毒：在变化的世界中的进化、生态学和公共卫生策略。2024 16,1688病毒。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-16 DOI: 10.3390/v17070992

Francesco Branda, Grazia Pavia, Alessandra Ciccozzi, Angela Quirino, Nadia Marascio, Giovanni Matera, Chiara Romano, Chiara Locci, Ilenia Azzena, Noemi Pascale, Daria Sanna, Marco Casu, Giancarlo Ceccarelli, Massimo Ciccozzi, Fabio Scarpa

引用次数: 0