Viruses-Basel最新文献_第3页

Clinical Features of Human Parvovirus B19-Associated Encephalitis Identified in the Dakar Region, Senegal, and Viral Genome Characterization.

IF 3.8 3区医学

Viruses-Basel Pub Date : 2025-01-15 DOI: 10.3390/v17010111

Al Ousseynou Seye, Fatou Kiné Top, Maimouna Mbanne, Moussa Moise Diagne, Ousmane Faye, Amadou Alpha Sall, Ndongo Dia, Jean-Michel Heraud, Martin Faye

{"title":"Clinical Features of Human Parvovirus B19-Associated Encephalitis Identified in the Dakar Region, Senegal, and Viral Genome Characterization.","authors":"Al Ousseynou Seye, Fatou Kiné Top, Maimouna Mbanne, Moussa Moise Diagne, Ousmane Faye, Amadou Alpha Sall, Ndongo Dia, Jean-Michel Heraud, Martin Faye","doi":"10.3390/v17010111","DOIUrl":"10.3390/v17010111","url":null,"abstract":"Neurological manifestations associated with human parvovirus B19 (B19V) infections are rare and varied. Acute encephalitis and encephalopathy are the most common, accounting for 38.8% of all neurological manifestations associated with human B19V. Herein, we report on the clinical features of 13 laboratory-confirmed human cases of B19V-associated encephalitis in Senegal in the framework of a hospital-based surveillance of acute viral encephalitis conducted from 2021 to 2023. Overall, B19V was detected from 13 cerebrospinal fluid samples using specific real time PCR. The mean age was 16.7 years among B19V-positive patients, with a higher prevalence in 0-5-year-old children and the sex ratio (male/female) was 2.25. The B19V-positive patients mainly exhibited hypoleukocytosis, normal glycorrhachia, and normal proteinorrachia in the cerebrospinal fluid. While the main neurological symptoms included meningeal and infectious syndromes. Furthermore, three complete B19V genome sequences were successfully characterized using next-generation sequencing. The newly characterized sequences belonged to the genotype 1a and represent, to date, the first complete B19V genome sequences from Senegal. These sequences could be useful not only in future phylodynamic studies of B19V but also in the development of prevention or treatment countermeasures. Our study is noteworthy for the identification of acute B19V-associated encephalitis in Senegal More investigations on the risk factors associated with B19V transmission in Africa are warranted.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11769063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting EBV Episome for Anti-Cancer Therapy: Emerging Strategies and Challenges.

IF 3.8 3区医学

Viruses-Basel Pub Date : 2025-01-15 DOI: 10.3390/v17010110

Febri Gunawan Sugiokto, Renfeng Li

引用次数: 0

Klebsiella pneumoniae Phage M198 and Its Therapeutic Potential.

IF 3.8 3区医学

Viruses-Basel Pub Date : 2025-01-15 DOI: 10.3390/v17010115

Lika Leshkasheli, Ia Kusradze, Darejan Bolkvadze, Lia Askilashvili, Maria Chichashvili, Giorgi Tsertsvadze, Elisabed Zaldastanishvili

{"title":"Klebsiella pneumoniae Phage M198 and Its Therapeutic Potential.","authors":"Lika Leshkasheli, Ia Kusradze, Darejan Bolkvadze, Lia Askilashvili, Maria Chichashvili, Giorgi Tsertsvadze, Elisabed Zaldastanishvili","doi":"10.3390/v17010115","DOIUrl":"10.3390/v17010115","url":null,"abstract":"The rapid worldwide spread of antibiotic resistance is quickly becoming an increasingly concerning problem for human healthcare. Non-antibiotic antibacterial agents are in high demand for many Gram-negative bacterial pathogens, including Klebsiella pneumoniae. Klebsiella-targeting phages are among the most promising alternative therapy options. They have already been successfully applied in a number of cases, and it is expected that the need for anti-Klebsiella phages will only increase in the future. This prospect highlights the need for well-characterized therapeutic phages. In this work, we describe a K. pneumoniae phage, which also infects strains of Klebsiella oxytoca. Here, we characterize phage M198 in terms of its biological and genetic properties. Since in some phage therapy cases, phages are administered in combination with antibiotics, here, we also screen for possible synergistic effects of combining phage M198 with six different antibiotics. We found that phage M198 has good lytic activity against clinical isolates; it does not have any indications of a temperate lifestyle, and it has synergistic potential when combined with some therapeutically relevant antibiotics.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11768853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inovirus-Encoded Peptides Induce Specific Toxicity in Pseudomonas aeruginosa.

IF 3.8 3区医学

Viruses-Basel Pub Date : 2025-01-15 DOI: 10.3390/v17010112

Juehua Weng, Yunxue Guo, Jiayu Gu, Ran Chen, Xiaoxue Wang

{"title":"Inovirus-Encoded Peptides Induce Specific Toxicity in Pseudomonas aeruginosa.","authors":"Juehua Weng, Yunxue Guo, Jiayu Gu, Ran Chen, Xiaoxue Wang","doi":"10.3390/v17010112","DOIUrl":"10.3390/v17010112","url":null,"abstract":"Pseudomonas aeruginosa is a common opportunistic pathogen associated with nosocomial infections. The primary treatment for infections typically involves antibiotics, which can lead to the emergence of multidrug-resistant strains. Therefore, there is a pressing need for safe and effective alternative methods. Phage therapy stands out as a promising approach. However, filamentous prophages (Pfs) commonly found in P. aeruginosa encode genes with phage defense activity, thereby reducing the efficacy of phage therapy. Through a genomic analysis of the Pf4 prophage, we identified a 102 bp gene co-transcribed with the upstream gene responsible for phage release (zot gene), giving rise to a 33-amino-acid polypeptide that we have named Pf4-encoded toxic polypeptide (PftP4). The overexpression of PftP4 demonstrated cellular toxicity in P. aeruginosa, with subcellular localization indicating its presence in the cell membrane and a subsequent increase in membrane permeability. Notably, PftP4 homologues are found in multiple Pf phages and exhibit specificity in their toxicity towards P. aeruginosa among the tested bacterial strains. Our study reveals that the novel Pf-encoded polypeptide PftP4 has the potential to selectively target and eradicate P. aeruginosa, offering valuable insights for combating P. aeruginosa infections.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11769263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic, Evolutionary, and Pathogenic Characterization of a New Polerovirus in Traditional Chinese Medicine Viola philippica.

IF 3.8 3区医学

Viruses-Basel Pub Date : 2025-01-15 DOI: 10.3390/v17010114

Yuanling Chen, Gaoxiang Chen, Jiaping Yu, Yali Zhou, Shifang Fei, Haorong Chen, Jianxiang Wu, Shuai Fu

{"title":"Genomic, Evolutionary, and Pathogenic Characterization of a New Polerovirus in Traditional Chinese Medicine Viola philippica.","authors":"Yuanling Chen, Gaoxiang Chen, Jiaping Yu, Yali Zhou, Shifang Fei, Haorong Chen, Jianxiang Wu, Shuai Fu","doi":"10.3390/v17010114","DOIUrl":"10.3390/v17010114","url":null,"abstract":"Viola philippica, a medicinal herbaceous plant documented in the Chinese Pharmacopoeia, is a promising candidate for research into plant-derived pharmaceuticals. However, the study of newly emerging viruses that threaten the cultivation of V. philippica remains limited. In this study, V. philippica plants exhibiting symptoms such as leaf yellowing, mottled leaves, and vein chlorosis were collected and subjected to RNA sequencing to identify potential viral pathogens. A novel polerovirus, named Viola Philippica Polerovirus (VPPV), was identified in V. philippica. VPPV possesses a linear, positive-sense, single-stranded RNA genome consisting of 5535 nucleotides (nt) and encodes seven highly overlapping open reading frames (ORFs). Two potential recombination events were identified within ORF2, ORF3a, and ORF3, providing insights into the genetic diversity and evolution history of this novel polerovirus. An infectious cDNA clone of VPPV was successfully constructed and shown to infect Nicotiana benthamiana. Using a PVX-based heterologous expression system, the VPPV P0 protein was shown to trigger a systemic hypersensitive response (HR)-like reaction in N. benthamiana, indicating that P0 functions as the main pathogenicity determinant. These findings contributed to the detection and understanding of pathogenic mechanisms and control strategies for VPPV in V. philippica.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11768663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interpretation of COVID-19 Epidemiological Trends in Mexico Through Wastewater Surveillance Using Simple Machine Learning Algorithms for Rapid Decision-Making.

IF 3.8 3区医学

Viruses-Basel Pub Date : 2025-01-15 DOI: 10.3390/v17010109

Arnoldo Armenta-Castro, Orlando de la Rosa, Alberto Aguayo-Acosta, Mariel Araceli Oyervides-Muñoz, Antonio Flores-Tlacuahuac, Roberto Parra-Saldívar, Juan Eduardo Sosa-Hernández

{"title":"Interpretation of COVID-19 Epidemiological Trends in Mexico Through Wastewater Surveillance Using Simple Machine Learning Algorithms for Rapid Decision-Making.","authors":"Arnoldo Armenta-Castro, Orlando de la Rosa, Alberto Aguayo-Acosta, Mariel Araceli Oyervides-Muñoz, Antonio Flores-Tlacuahuac, Roberto Parra-Saldívar, Juan Eduardo Sosa-Hernández","doi":"10.3390/v17010109","DOIUrl":"10.3390/v17010109","url":null,"abstract":"Detection and quantification of disease-related biomarkers in wastewater samples, denominated Wastewater-based Surveillance (WBS), has proven a valuable strategy for studying the prevalence of infectious diseases within populations in a time- and resource-efficient manner, as wastewater samples are representative of all cases within the catchment area, whether they are clinically reported or not. However, analysis and interpretation of WBS datasets for decision-making during public health emergencies, such as the COVID-19 pandemic, remains an area of opportunity. In this article, a database obtained from wastewater sampling at wastewater treatment plants (WWTPs) and university campuses in Monterrey and Mexico City between 2021 and 2022 was used to train simple clustering- and regression-based risk assessment models to allow for informed prevention and control measures in high-affluence facilities, even if working with low-dimensionality datasets and a limited number of observations. When dividing weekly data points based on whether the seven-day average daily new COVID-19 cases were above a certain threshold, the resulting clustering model could differentiate between weeks with surges in clinical reports and periods between them with an 87.9% accuracy rate. Moreover, the clustering model provided satisfactory forecasts one week (80.4% accuracy) and two weeks (81.8%) into the future. However, the prediction of the weekly average of new daily cases was limited (R2 = 0.80, MAPE = 72.6%), likely because of insufficient dimensionality in the database. Overall, while simple, WBS-supported models can provide relevant insights for decision-makers during epidemiological outbreaks, regression algorithms for prediction using low-dimensionality datasets can still be improved.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11768489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the Diversity of Actinobacterial Tectiviridae: A Novel Genus from Microbacterium.

IF 3.8 3区医学

Viruses-Basel Pub Date : 2025-01-15 DOI: 10.3390/v17010113

Jacqueline M Washington, Holly Basta, Angela Bryanne De Jesus, Madison G Bendele, Steven G Cresawn, Emily K Ginser

{"title":"Expanding the Diversity of Actinobacterial Tectiviridae: A Novel Genus from Microbacterium.","authors":"Jacqueline M Washington, Holly Basta, Angela Bryanne De Jesus, Madison G Bendele, Steven G Cresawn, Emily K Ginser","doi":"10.3390/v17010113","DOIUrl":"10.3390/v17010113","url":null,"abstract":"Six novel Microbacterium phages belonging to the Tectiviridae family were isolated using Microbacterium testaceum as a host. Phages MuffinTheCat, Badulia, DesireeRose, Bee17, SCoupsA, and LuzDeMundo were purified from environmental samples by students participating in the Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program at Alliance University, New York. The phages have linear dsDNA genomes 15,438-15,636 bp with 112-120 bp inverted terminal repeats. Transmission electron microscopy (TEM) imaging analysis revealed that the six novel phages have six-sided icosahedral double-layered capsids with an internal lipid membrane that occasionally forms protruding nanotubules. Annotation analysis determined that the novel Microbacterium phages all have 32-34 protein-coding genes and no tRNAs. Like other Tectiviridae, the phage genomes are arranged into two segments and include three highly conserved family genes that encode a DNA polymerase, double jelly-roll major capsid protein, and packaging ATPase. Although the novel bacteriophages have 91.6 to 97.5% nucleotide sequence similarity to each other, they are at most 58% similar to previously characterized Tectiviridae genera. Consequently, these novel Microbacterium phages expand the diversity of the Tectiviridae family, and we propose they form the sixth genus, Zetatectivirus.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11768872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the First Marine Pestivirus, Phocoena Pestivirus (PhoPeV).

IF 3.8 3区医学

Viruses-Basel Pub Date : 2025-01-14 DOI: 10.3390/v17010107

Lars Söder, Denise Meyer, Olaf Isken, Norbert Tautz, Matthias König, Alexander Postel, Paul Becher

{"title":"Characterization of the First Marine Pestivirus, Phocoena Pestivirus (PhoPeV).","authors":"Lars Söder, Denise Meyer, Olaf Isken, Norbert Tautz, Matthias König, Alexander Postel, Paul Becher","doi":"10.3390/v17010107","DOIUrl":"10.3390/v17010107","url":null,"abstract":"The first marine pestivirus, Phocoena pestivirus (PhoPeV), isolated from harbor porpoise, has been recently described. To further characterize this unique pestivirus, its host cell tropism and growth kinetics were determined in different cell lines. In addition, the interaction of PhoPeV with innate immunity in porcine epithelial cells and the role of selected cellular factors involved in the viral entry and RNA replication of PhoPeV were investigated in comparison to closely and distantly related pestiviruses. While Bungowannah pestivirus (BuPV), a unique porcine pestivirus closely related to PhoPeV, exhibits a broad cell tropism, PhoPeV only infects cells from pigs, cattle, sheep, and cats, as has been described for classical swine fever virus (CSFV). Viral titers correlate with the amount of intracellular PhoPeV-specific RNA detected in the tested cell lines. PhoPeV replicates most efficiently in the porcine kidney cell line SK6. Pestiviruses generally counteract the cellular innate immune response by degradation of interferon regulatory factor 3 (IRF3) mediated by the viral N-terminal protease (Npro). No degradation of IRF3 and an increased expression of the type 1 interferon-stimulated antiviral protein Mx1 was observed in porcine cells infected with PhoPeV whose genome lacks the Npro encoding region. Infection of a CD46-deficient porcine cell line suggested that CD46, which is implicated in the viral entry of several pestiviruses, is not a major factor for the viral entry of PhoPeV. Moreover, the results of this study confirmed that the cellular factor DNAJC14 plays a crucial role in viral RNA replication of non-cytopathic pestiviruses, including PhoPeV.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11768717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances of Avian Viruses Research.

IF 3.8 3区医学

Viruses-Basel Pub Date : 2025-01-14 DOI: 10.3390/v17010099

Chi-Young Wang

引用次数: 0

A Survey of Wild Indigenous Cryptostylis ovata Orchid Populations in Western Australia Reveals Spillover of Exotic Viruses.

IF 3.8 3区医学

Viruses-Basel Pub Date : 2025-01-14 DOI: 10.3390/v17010108

Stephen Wylie, Hua Li, Shu Hui Koh

{"title":"A Survey of Wild Indigenous Cryptostylis ovata Orchid Populations in Western Australia Reveals Spillover of Exotic Viruses.","authors":"Stephen Wylie, Hua Li, Shu Hui Koh","doi":"10.3390/v17010108","DOIUrl":"10.3390/v17010108","url":null,"abstract":"Cryptostylis ovata is a terrestrial orchid endemic to southwestern Australia. The virus status of C. ovata has not been studied. Eighty-three C. ovata samples from 16 populations were collected, and sequencing was used to identify RNA viruses from them. In one population, all tested plants were co-infected with isolates of the exotic-to-Australia viruses Ornithogalum mosaic virus (OrMV) and bean yellow mosaic virus (BYMV). In another population, one plant was infected with BYMV. No viruses were detected in the remaining populations. The OrMV isolate shared 98-99% nucleotide identity with isolates identified from wild indigenous Lachenalia (Iridaceae) plants in South Africa. This suggests that the source of OrMV in C. ovata may be one or more bulbous iridaceous flowering plants of southern African origin that were introduced to Western Australia as ornamentals and that have since become invasive weeds. One BYMV isolate from C. ovata also exhibited 99% nucleotide identity with strains isolated from the exotic leguminous crop Lupinus angustifolius in Western Australia, suggesting possible spillover to indigenous species from this source. This study with C. ovata highlights the probable role of invasive weeds and exotic crops as sources of exotic virus spillovers to indigenous plants.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11769163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0