Viruses-Basel最新文献_第2页

SARS-CoV-2 Neutralizing Antibodies 2.0. SARS-CoV-2 中和抗体 2.0。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-19 DOI: 10.3390/v16111791

Youchun Wang

引用次数: 0

Reexamination of the Sida Micrantha Mosaic Virus and Sida Mottle Virus Complexes: Classification Status, Diversity, Cognate DNA-B Components, and Host Spectrum. 重新审查 Sida Micrantha Mosaic Virus 和 Sida Mottle Virus 复合物：分类状况、多样性、同源 DNA-B 成分和宿主谱系。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-19 DOI: 10.3390/v16111796

Marcos Silva de Queiroz-Ferreira, Luciane de Nazaré Almeida Dos Reis, Maria Esther de Noronha Fonseca, Felipe Fochat Silva Melo, Ailton Reis, Leonardo Silva Boiteux, Rita de Cássia Pereira-Carvalho

{"title":"Reexamination of the Sida Micrantha Mosaic Virus and Sida Mottle Virus Complexes: Classification Status, Diversity, Cognate DNA-B Components, and Host Spectrum.","authors":"Marcos Silva de Queiroz-Ferreira, Luciane de Nazaré Almeida Dos Reis, Maria Esther de Noronha Fonseca, Felipe Fochat Silva Melo, Ailton Reis, Leonardo Silva Boiteux, Rita de Cássia Pereira-Carvalho","doi":"10.3390/v16111796","DOIUrl":"10.3390/v16111796","url":null,"abstract":"Sida mottle virus (SiMoV) and Sida micrantha mosaic virus (SiMMV) are major Brazilian begomoviruses (Geminiviridae). However, the range of DNA-A identity of isolates of these viruses (81-100%) is not in agreement with the current criteria for Begomovirus species demarcation (<91%). To clarify this putative classification problem, we performed a comprehensive set of molecular analyses with all 53 publicly available isolates (with complete DNA-A genomes) designated as either SiMoV or SiMMV (including novel isolates obtained herein from nationwide metagenomics-based studies). Two well-defined phylogenetic clusters were identified. The SiMMV complex (n = 47) comprises a wide range of strains (with a continuum variation of 88.8-100% identity) infecting members of five botanical families (Malvaceae, Solanaceae, Fabaceae, Oxalidaceae, and Passifloraceae). The SiMoV group now comprises eight isolates (90-100% identity) restricted to Malvaceae hosts, including one former reference SiMMV isolate (gb|NC_077711) and SP77 (gb|FN557522; erroneously named as \"true SiMMV\"). Iteron analyses of metagenomics-derived information allowed for the discovery of the missing DNA-B cognate of SiMoV (93.5% intergenic region identity), confirming its bipartite nature. Henceforth, the correct identification of SiMoV and SiMMV isolates will be a crucial element for effective classical and biotech resistance breeding of the viral host species.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond Antivirals: Alternative Therapies for Long COVID. 抗病毒药物之外：长 COVID 的替代疗法。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-19 DOI: 10.3390/v16111795

Achilleas Livieratos, Charalambos Gogos, Karolina Akinosoglou

{"title":"Beyond Antivirals: Alternative Therapies for Long COVID.","authors":"Achilleas Livieratos, Charalambos Gogos, Karolina Akinosoglou","doi":"10.3390/v16111795","DOIUrl":"10.3390/v16111795","url":null,"abstract":"Long COVID or Post-Acute Sequelae of SARS-CoV-2 infection (PASC) is a condition characterized by numerous lingering symptoms that persist for weeks to months following the viral illness. While treatment for PASC is still evolving, several therapeutic approaches beyond traditional antiviral therapies are being investigated, such as immune-modulating agents, anti-inflammatory drugs, and various supportive interventions focusing at alleviating symptoms and enhancing recovery. We aimed to summarize the breadth of available evidence, identify knowledge gaps, and highlight promising non-antiviral therapies for Long COVID/PASC. We followed the framework of a scoping methodology by mapping existing evidence from a range of studies, including randomized clinical trials, observational research, and case series. Treatments evaluated include metformin, low-dose naltrexone (LDN), dexamethasone, statins, omega-3 fatty acids, L-arginine, and emerging therapies like intravenous immunoglobulin (IVIg) and therapeutic apheresis. Early findings suggest that metformin has the strongest clinical evidence, particularly from large phase 3 trials, while LDN and dexamethasone show potential based on observational studies. However, many treatments lack robust, large-scale trials. This review emphasizes the need for further research to confirm the efficacy of these treatments and guide clinical practice for Long COVID management.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interest and Expectations for a Herpes Vaccine Among People Diagnosed with Genital HSV 1-2 Infection: Results from an Italian Survey. 确诊为生殖器 HSV 1-2 感染者对疱疹病毒疫苗的兴趣和期望：意大利调查的结果。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-18 DOI: 10.3390/v16111789

Lovel Lisac, Angelo Roberto Raccagni, Riccardo Lolatto, Flavia Passini, Chiara Maci, Elena Bruzzesi, Nicolò Moschetta, Antonella Castagna, Silvia Nozza

{"title":"Interest and Expectations for a Herpes Vaccine Among People Diagnosed with Genital HSV 1-2 Infection: Results from an Italian Survey.","authors":"Lovel Lisac, Angelo Roberto Raccagni, Riccardo Lolatto, Flavia Passini, Chiara Maci, Elena Bruzzesi, Nicolò Moschetta, Antonella Castagna, Silvia Nozza","doi":"10.3390/v16111789","DOIUrl":"10.3390/v16111789","url":null,"abstract":"Genital herpes simplex virus (HSV) is associated with a reduction in quality of life and adverse outcomes. The aim of this study is to assess the interest and expectations for a therapeutic HSV vaccine among individuals diagnosed with genital herpes in Italy. A retrospective survey was conducted at the Infectious Diseases Unit of the IRCCS San Raffaele Scientific Institute, Milan, Italy. The study collected data on demographics, clinical history and interest in HSV vaccination. The results showed that 87.5% of participants were interested in a therapeutic vaccine, with interest higher among younger people and those with frequent genital herpes recurrences. Participants most expected the vaccine to reduce the pain associated with outbreaks, followed by a reduction in the frequency and duration of recurrences. These findings underscore the strong demand for a therapeutic HSV vaccine, especially among those who experience recurrent outbreaks, and highlight the importance of considering patient expectations when developing preventive and therapeutic strategies for genital herpes.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breaching the Barrier: Investigating Initial Herpes Simplex Viral Infection and Spread in Human Skin and Mucosa. 突破屏障：调查人类皮肤和粘膜的初始单纯疱疹病毒感染和传播。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-18 DOI: 10.3390/v16111790

Hafsa Rana, Naomi R Truong, Dona R Sirimanne, Anthony L Cunningham

{"title":"Breaching the Barrier: Investigating Initial Herpes Simplex Viral Infection and Spread in Human Skin and Mucosa.","authors":"Hafsa Rana, Naomi R Truong, Dona R Sirimanne, Anthony L Cunningham","doi":"10.3390/v16111790","DOIUrl":"10.3390/v16111790","url":null,"abstract":"Herpes simplex virus (HSV) is sexually transmitted via the anogenital mucosa where it initially infects epidermal keratinocytes and mononuclear phagocytes (MNPs). It then spreads to the dorsal root ganglion via sensory nerve endings, to remain latent for life with periodic reactivation. Currently, there is no cure or vaccine. Initial or recurrent HSV infection can produce serious complications and mediate acquisition of HIV. This review outlines the initial events after the HSV infection of human anogenital mucosa to determine the optimal window to target the virus before it becomes latent. After infection, HSV spreads rapidly within the mid-layers of epidermal keratinocytes in the explanted human inner foreskin. Infected cells produce chemokines, which modulate nectin-1 distribution on the surface of adjacent keratinocytes, facilitating viral spread. Epidermal Langerhans cells and dendritic cells become infected with HSV followed by a \"viral relay\" to dermal MNPs, which then present viral antigen to T cells in the dermis or lymph nodes. These data indicate the need for interruption of spread within 24 h by diffusible vaccine-induced mediators such as antiviral cytokines from resident immune cells or antibodies. Intradermal/mucosal vaccines would need to target the relevant dermal MNPs to induce HSV-specific CD4+ and CD8+ T cells.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special Issue: Research on Herpes Virus Fusion and Entry. 特刊：疱疹病毒融合与进入研究。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-18 DOI: 10.3390/v16111788

Doina Atanasiu, Tina M Cairns

引用次数: 0

Respiratory Viral Infection Patterns in Hospitalised Children Before and After COVID-19 in Hong Kong. 香港住院儿童在 COVID-19 前后的呼吸道病毒感染模式。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-17 DOI: 10.3390/v16111786

Jason Chun Sang Pun, Kin Pong Tao, Stacy Lok Sze Yam, Kam Lun Hon, Paul Kay Sheung Chan, Albert Martin Li, Renee Wan Yi Chan

{"title":"Respiratory Viral Infection Patterns in Hospitalised Children Before and After COVID-19 in Hong Kong.","authors":"Jason Chun Sang Pun, Kin Pong Tao, Stacy Lok Sze Yam, Kam Lun Hon, Paul Kay Sheung Chan, Albert Martin Li, Renee Wan Yi Chan","doi":"10.3390/v16111786","DOIUrl":"10.3390/v16111786","url":null,"abstract":"The study highlights the significant changes in respiratory virus epidemiology following the lifting of COVID-19 restrictions.Method: In this single-centre retrospective study, the virological readouts of adenovirus (AdV), influenza virus A (IAV), influenza virus B (IBV), parainfluenza viruses (PIV) 1, 2, 3, 4, respiratory syncytial virus (RSV), and coupled enterovirus and rhinovirus (EV/RV) were extracted from the respiratory specimens of paediatric patients in Hong Kong from January 2015 to February 2024. The subjects were stratified into five age groups.Results: The study included 18,737 and 6001 respiratory specimens in the pre-COVID-19 and post-COVID-19 mask mandate period, respectively. The mean age of hospitalised patients increased from 3.49 y ± 0.03 y to 4.37 y ± 0.05 y after the COVID-19 lockdown. The rates of single-virus infection and co-infection were significantly higher in the post-COVID-19 mask mandate period. The odds ratio for AdV for all age groups (OR: 4.53, 4.03, 2.32, 2.46, 1.31) and RSV in older children from 3 years old and above (OR: 1.95, 3.38, p < 0.01) were significantly elevated after the COVID-19 outbreak.Conclusions: Our findings suggest that public health measures to contain COVID-19 may have unintended consequences on children's natural exposure and immunity to other respiratory viruses, potentially increasing their morbidity in the post-pandemic era.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IPEC-J2 Autophagy Induced by TLR4 and NSP6 Interactions Facilitate Porcine Epidemic Diarrhea Virus Replication. TLR4和NSP6相互作用诱导的IPEC-J2自噬促进猪流行性腹泻病毒的复制

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-17 DOI: 10.3390/v16111787

Haiyuan Zhao, Dianzhong Zheng, Qinyuan Chang, Hailin Zhang, Yilan Shao, Jiaxuan Li, Wen Cui, Yanping Jiang, Lijie Tang, Yijing Li, Xiaona Wang

{"title":"IPEC-J2 Autophagy Induced by TLR4 and NSP6 Interactions Facilitate Porcine Epidemic Diarrhea Virus Replication.","authors":"Haiyuan Zhao, Dianzhong Zheng, Qinyuan Chang, Hailin Zhang, Yilan Shao, Jiaxuan Li, Wen Cui, Yanping Jiang, Lijie Tang, Yijing Li, Xiaona Wang","doi":"10.3390/v16111787","DOIUrl":"10.3390/v16111787","url":null,"abstract":"Autophagy is an important cellular response against intracellular pathogens. However, some viruses have evolved mechanisms to hijack this defensive process to provide favorable conditions for virus replication in host cells. The porcine epidemic diarrhea virus (PEDV) has been shown to alter autophagy pathways; however, it is still unknown through which receptors PEDV induces autophagy in IPEC-J2 cells, whether autophagy facilitates PEDV replication, and which functional domains of PEDV proteins are primarily responsible for inducing autophagy. Here, we found that PEDV infection induces autophagy in host cells via distinct and uncoupled molecular pathways. RNA-seq technology was used to analyze the expression patterns of intracellular genes in PEDV-infected IPEC-J2 cells using transcriptomics. The results demonstrate that PEDV triggers autophagy via the cellular pathogen receptor TLR4 and the AKT-mTOR pathway. As evidenced by autophagosome detection, PEDV infection increases autophagosomes and light chain 3 (LC3)-II as well as downregulated AKT-mTOR phosphorylation. Our study revealed that the binding of the viral protein NSP61-2C (56-151aa) to TLR4 triggers autophagy and inactivates the AKT-mTOR pathway, both of which are critical for facilitating PEDV infection. Through screening and analysis, TLR4 was found to be a key gene involved in PEDV-induced autophagy. The screening of the key functional domains of NSP6 (56-151aa) for their ability to induce autophagy in IPEC-J2 cells provided a basis for the in-depth analysis of the pathogenic mechanism of PEDV infection-induced autophagy and promotion of self-replication and also provided an important target for the study of PEDV antiviral drugs. In conclusion, we elucidated that the PEDV infection of IPEC-J2 cells could induce autophagy and found that PEDV could use autophagy to promote its own replication.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Vesicles in Viral Liver Diseases. 病毒性肝病中的细胞外小泡

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-17 DOI: 10.3390/v16111785

Elias Kouroumalis, Ioannis Tsomidis, Argyro Voumvouraki

引用次数: 0

HSPA4 Enhances BRSV Entry via Clathrin-Mediated Endocytosis Through Regulating the PI3K-Akt Signaling Pathway and ATPase Activity of HSC70. HSPA4通过调节PI3K-Akt信号通路和HSC70的ATPase活性，增强了BRSV通过Clathrin介导的内吞作用进入细胞。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-17 DOI: 10.3390/v16111784

Yang Liu, Qiongyi Li, Shuai Shao, Xiaolan Ji, Wanning Gao, Yiyang Fan, Mingqi Liu, Yan Wang, Jialin Bai

{"title":"HSPA4 Enhances BRSV Entry via Clathrin-Mediated Endocytosis Through Regulating the PI3K-Akt Signaling Pathway and ATPase Activity of HSC70.","authors":"Yang Liu, Qiongyi Li, Shuai Shao, Xiaolan Ji, Wanning Gao, Yiyang Fan, Mingqi Liu, Yan Wang, Jialin Bai","doi":"10.3390/v16111784","DOIUrl":"10.3390/v16111784","url":null,"abstract":"Bovine respiratory syncytial virus (BRSV) is an enveloped RNA virus that utilizes clathrin-mediated endocytosis for cell entry and is a significant pathogen in bovine respiratory disease (BRD). Heat shock protein family A member 4 (HSPA4), a member of the HSP70 family, is known to be involved in the progression of various cancers. However, its role in virus entry has not been previously explored. Through experiments involving Western blot analysis, virus titer, and virus copies analysis, we demonstrated that HSPA4 can regulate BRSV entry and replication. The specific regulation mode is to enhance BRSV entry by promoting clathrin-mediated endocytosis. We used Western blot, virus titer, virus copies analysis, and IFA to demonstrate that HSPA4 can promote clathrin heavy chain protein (CHC) expression and further promote BRSV entry by activating the PI3K-Akt signaling pathway. Furthermore, we observed that HSPA4 boosts the efficiency of clathrin-mediated endocytosis by increasing the ATPase activity of heat shock cognate protein 70 (HSC70), thereby facilitating BRSV entry. Additionally, our investigation into the impact of HSPA4 on the entry of other viruses revealed that HSPA4 can facilitate the entry of a variety of viruses into host cells.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0