Viruses-Basel最新文献

{"title":"Discovery and Genome Characterization of Three New Rhabdoviruses Infecting <i>Passiflora</i> spp. in Brazil.","authors":"Andreza Henrique Vidal, Ana Clara Rodrigues Abreu, Jorge Flávio Sousa Dantas-Filho, Monique Jacob Xavier Vianna, Cristiano Lacorte, Emanuel Felipe Medeiros Abreu, Gustavo Pereira Felix, Dione Mendes Teixeira Alves-Freitas, Bruna Pinheiro-Lima, Isadora Nogueira, Fabio Gelape Faleiro, Raul Castro Carriello Rosa, Onildo Nunes Jesus, Marcio Martinello Sanches, Yam Sousa Santos, Rosana Blawid, José Leonardo Santos Jiménez, Maite Freitas Silva Vaslin, Elliot Watanabe Kitajima, Magnolia de Araujo Campos, Rafaela Salgado Fontenele, Arvind Varsani, Fernando Lucas Melo, Simone Graça Ribeiro","doi":"10.3390/v17050725","DOIUrl":"10.3390/v17050725","url":null,"abstract":"<p><p>This study aimed to explore the RNA viruses affecting <i>Passiflora</i> species in Brazil. Our results enhance the understanding of the viruses that infect <i>Passiflora</i> plants by identifying and characterizing three previously unrecognized viruses: Passiflora cytorhabdovirus (PFCV), Passiflora nucleorhabdovirus 1 (PaNV1), and Passiflora nucleorhabdovirus 2 (PaNV2). These rhabdoviruses were identified through high-throughput sequencing and validated by reverse transcription-polymerase chain reaction (RT-PCR) in various <i>Passiflora</i> species. PFCV has a genome organization 3'-N-P-P3-P4-M-G-P7-L-5' and was classified as a novel member of the <i>Gammacytorhabdovirus</i> genus. A particularly noteworthy feature of PFCV is its glycoprotein, as the genomes of other gammarhabdoviruses do not contain this gene. PFCV has a high incidence across multiple locations and was identified in plants from Northeastern, Central, and Southeastern Brazil. PaNV1 with genome structure 3'-N-P-P3-M-G-L-5' and PaNV2 with genome organization 3'-N-X-P-Y-M-G-L-5' are new members of the <i>Alphanucleorhabdovirus</i> genus and have a more restricted occurrence. Importantly, all three viruses were found in mixed infections alongside at least one other virus. In situ observations confirmed mixed infections, with PaNV2 particles co-located in tissues with a potyvirus and a carlavirus. Phylogenetic and glycoprotein sequence similarity network analysis provided insights into their evolutionary placement and potential vector associations. These findings expand the known diversity of rhabdoviruses in <i>Passiflora</i> and contribute to the understanding of their evolution and epidemiology.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering SARS-CoV-2 Molecular Epidemiology Across the Pandemic Transition: Insights into Transmission in Clinical and Environmental Samples.","authors":"Vrushali D Patil, Rashmi Chowdhary, Anvita Gupta Malhotra, Jitendra Singh, Debasis Biswas, Rajnish Joshi, Jagat Rakesh Kanwar","doi":"10.3390/v17050726","DOIUrl":"10.3390/v17050726","url":null,"abstract":"<p><strong>Background: </strong>Respiratory droplets are the main way in which the COVID-19 pandemic's causal agent, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), spreads. Angiotensin-converting enzyme 2 (ACE2) receptors, especially in lung cells, allow the virus to enter host cells. However, ACE2 expression in intestinal cells has sparked worries about possible fecal transfer, particularly in poor-sanitation areas like India.</p><p><strong>Methods: </strong>Between July 2021 and July 2024, clinical (nasopharyngeal, saliva, and stool samples) and sewage samples were collected from outpatient departments and sewage treatment plants (STPs), respectively, from the high-population-density area under study in order to investigate SARS-CoV-2 transmission.</p><p><strong>Results: </strong>This proof-of-concept study analyzed clinical samples from <i>n</i> = 60 COVID-19-positive patients at a central Indian tertiary care hospital and <i>n</i> = 156 samples from hospital STPs. Variants of SARS-CoV-2 were found using qRT-PCR and Next-Generation Sequencing (NGS). Of the <i>n</i> = 37 qRT-PCR-positive patients who gave their assent, 30% had stool samples that tested positive for viral RNA. In 70% of positive NP and 65% of positive saliva samples, along with two stool samples from immunocompromised patients, the live virus was identified using Vero E6 cell lines. Although 18% of the tests reported qRT-PCR-positive results, no live virus was detected in sewage samples despite NGS validation. The detection of SARS-CoV-2 in the absence of confirmed clinical cases may indicate the silent circulation of the virus within the community, suggesting that sewage surveillance can serve as an early warning system before an outbreak occurs.</p><p><strong>Conclusions: </strong>These findings provide critical insights into the importance of continuous environmental surveillance, silent virus circulation, changes in viral epidemiology throughout the years, and strategies to mitigate coronavirus outbreaks.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of TDP-43 in SARS-CoV-2-Related Neurodegenerative Changes.","authors":"Dong-Hwi Kim, Jae-Hyeong Kim, Min-Tae Jeon, Kyu-Sung Kim, Do-Geun Kim, In-Soo Choi","doi":"10.3390/v17050724","DOIUrl":"10.3390/v17050724","url":null,"abstract":"<p><p>The coronavirus disease 2019 (COVID-19) pandemic has been linked to long-term neurological effects with multifaceted complications of neurodegenerative diseases. Several studies have found that pathological changes in transactive response DNA-binding protein of 43 kDa (TDP-43) are involved in these cases. This review explores the causal interactions between severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and TDP-43 from multiple perspectives. Some viral proteins of SARS-CoV-2 have been shown to induce pathological changes in TDP-43 through its cleavage, aggregation, and mislocalization. SARS-CoV-2 infection can cause liquid-liquid phase separation and stress granule formation, which accelerate the condensation of TDP-43, resulting in host RNA metabolism disruption. TDP-43 has been proposed to interact with SARS-CoV-2 RNA, though its role in viral replication remains to be fully elucidated. This interaction potentially facilitates viral replication, while viral-induced oxidative stress and protease activity accelerate TDP-43 pathology. Evidence from both clinical and experimental studies indicates that SARS-CoV-2 infection may contribute to long-term neurological sequelae, including amyotrophic lateral sclerosis-like and frontotemporal dementia-like features, as well as increased phosphorylated TDP-43 deposition in the central nervous system. Biomarker studies further support the link between TDP-43 dysregulation and neurological complications of long-term effects of COVID-19 (long COVID). In this review, we presented a novel integrative framework of TDP-43 pathology, bridging a gap between SARS-CoV-2 infection and mechanisms of neurodegeneration. These findings underscore the need for further research to clarify the TDP-43-related neurodegeneration underlying SARS-CoV-2 infection and to develop therapeutic strategies aimed at mitigating long-term neurological effects in patients with long COVID.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Pathogenicity of a Natural Recombinant Pig Reproductive and Respiratory Syndrome Virus in Northeast China.","authors":"Zhixin Tian, Qiwei Li, Luxiang Xu, Dexin Liang, Yuan Li, Ziqi Shi, Lingzhi Luo, Jiechao Jin, Xiaoyi Huo, Xiumei Dong, Han Zhou","doi":"10.3390/v17050729","DOIUrl":"10.3390/v17050729","url":null,"abstract":"<p><p>First reported in 1987, the porcine reproductive and respiratory syndrome virus (PRRSV) has significantly disrupted the major regions affected by PRRSV in the pig breeding industry. Recently, outbreaks of disease caused by recombinant PRRSV strains in China have raised serious concerns. Effective immunization and infection control in pig populations is critical, as the virus frequently undergoes mutation and recombination. This study characterized a novel recombinant PRRSV strain, BX/CH/22, isolated from Northeast China. Genetic analysis revealed that BX/CH/22 is a recombinant of JXA1, NADC 30-like, and NADC 34-like strains. Phylogenetic analysis of the non-structural protein (NSP) 2 region classified BX/CH/22 as JXA1 PRRSV-like, with a characteristic deletion of 30 discontinuous amino acids in NSP2. However, Open Reading Frame (ORF) 5 analysis classified it as NADC 30-like PPRSV, while whole-genome phylogenetic analysis classified it as NADC 34-like PPRSV. Recombination analysis revealed that BX/CH/22 contains an NADC 34-like PRRSV backbone, an NSP-coding region from NADC 30-like PRRSV, and an ORF2-ORF6 region from NADC 34-like PRRSV. The strain was isolated from serum samples obtained from commercial swine farms undergoing active PRRS outbreaks. In animal experiments, all BX/CH/22-challenged piglets exhibited persistent fever, with peak temperatures >40.5 °C at 4-9 dpi resolving by 11 dpi, accompanied by cough, anorexia, and lethargy. A significant reduction in daily weight gain was observed in infected groups compared to asymptomatic controls, with a 100% survival rate. Our findings provide early warning for PRRSV immune control strategies.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Neutralizing Antibodies in Hantavirus-Infected Patients Using Authentic Virus and Recombinant Vesicular Stomatitis Virus Systems.","authors":"Punya Shrivastava-Ranjan, Jamie A Kelly, Laura K McMullan, Deborah Cannon, Laura Morgan, Payel Chatterjee, Shilpi Jain, Joel M Montgomery, Mike Flint, César G Albariño, Christina F Spiropoulou","doi":"10.3390/v17050723","DOIUrl":"10.3390/v17050723","url":null,"abstract":"<p><p>Hantaviruses, including the Sin Nombre virus (SNV) and Andes virus (ANDV), are associated with severe global health risks, causing high mortality rates in hantavirus pulmonary syndrome (HPS) patients. Neutralizing antibodies are essential for virus clearance and survival, making neutralization assays critical for understanding immunity and evaluating therapeutic strategies. In this study, we developed a recombinant vesicular stomatitis virus (VSV)-based surrogate system expressing SNV and ANDV glycoproteins (GPCs), enabling neutralization studies under biosafety level 2 conditions. The neutralization titers obtained with the VSV-based system closely matched the findings from authentic hantavirus assays performed under biosafety level 3 conditions, confirming its potential as a useful tool for determining immune responses and advancing hantavirus research.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of Antiviral Drug Resistance in SARS-CoV-2.","authors":"Roy Dinata, Piyush Baindara, Santi M Mandal","doi":"10.3390/v17050722","DOIUrl":"10.3390/v17050722","url":null,"abstract":"<p><p>The COVID-19 pandemic has had a significant impact and continues to alarm the entire world due to the rapid emergence of new variants, even after mass vaccinations. There is still an urgent need for new antivirals or strategies to combat the SARS-CoV-2 infections; however, we have success stories with nirmatrelvir. Drug repurposing and drug discovery may lead to a successful SARS-CoV-2 antiviral; however, rapid drug use may cause unexpected mutations and antiviral drug resistance. Conversely, novel variants of the SARS-CoV-2 can diminish the neutralizing efficacy of vaccines, thereby enhancing viral fitness and increasing the likelihood of drug resistance emergence. Additionally, the disposal of antivirals in wastewater also contributes to drug resistance. Overall, the present review summarizes the strategies and mechanisms involved in the development of drug resistance in SARS-CoV-2. Understanding the mechanism of antiviral resistance is crucial to mitigate the significant healthcare threat and to develop effective therapeutics against drug resistance.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foscarnet Versus Ganciclovir for Severe Congenital Cytomegalovirus Infection: Short- and Long-Term Follow-Up.","authors":"Giovanni Nigro, Marta Buzzi, Milena Catenaro, Eleonora Coclite, Mario Muselli","doi":"10.3390/v17050720","DOIUrl":"10.3390/v17050720","url":null,"abstract":"<p><strong>Background: </strong>Cytomegalovirus (CMV) infection is the most common and serious congenital infection, with universal screening in pregnancy, standardized therapy, and a vaccine still lacking.</p><p><strong>Study design: </strong>In the 1990s, we noted that intravenous ganciclovir did not cure some children with severe sequelae due to congenital cytomegalovirus (CMV) infection. Therefore, we performed an open randomized trial using intravenous foscarnet as an alternative to intravenous ganciclovir in 24 infants (12 in each therapy group), all with severe neurological manifestations due to congenital CMV infection. Nine and five infants, belonging to the foscarnet or ganciclovir group, respectively, had abnormal hearing. One infant in each group also had chorioretinitis. Concomitantly, 12 CMV-infected infants with similar manifestations, who did not receive any therapy, were used as controls. The results of short-term (2 years) and long-term (7-29 years, mean 22.2) follow-up are reported herein. Short-term results: Neurological outcomes were normal in five of the twelve children who were treated with foscarnet, compared to nine of the twelve children given ganciclovir. None of the untreated children were healthy. There was a statistically significant difference (<i>p</i> = 0.023) between the treated and untreated children. Hearing was normal in four of the twelve children treated with foscarnet, seven of the twelve children treated with ganciclovir, and two untreated children. Long-term-results: Two children in both therapy groups died before the age of 17 years, and six untreated children died between 7 and 26 years of age. Neurological outcomes were normal in three of the ten children treated with foscarnet, in two of the ten treated with ganciclovir, and in none of the untreated children. Hearing was normal in two children treated with foscarnet, in six children treated with ganciclovir, and in one untreated child.</p><p><strong>Conclusions: </strong>Intravenous ganciclovir and foscarnet were found to be safe at long-term follow-up and appeared to be capable of mitigating the neurological and auditory consequences of congenital CMV disease at the short-term follow-up. However, there was progressive worsening of the symptomatology in all three groups, with a statistically significant increase in the number of deaths (<i>p</i> = 0.035) among 4 of the 24 children in the therapy groups and 6 of the 12 untreated children.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and Implementation of the Point-of-Care RT-RAA-CRISPR/Cas13a Diagnostic Test for Foot-And-Mouth Disease Virus Serotype O in Pigs.","authors":"Ping Meng, Bo Ni, Chenyu Li, Zhou Sha, Chunju Liu, Weijie Ren, Rong Wei, Fuxiao Liu, Jinming Li, Zhiliang Wang","doi":"10.3390/v17050721","DOIUrl":"10.3390/v17050721","url":null,"abstract":"<p><p>Foot and mouth disease virus (FMDV) is a highly pathogenic virus that mainly infects cloven hooved animals, such as pigs. The establishment of a rapid, sensitive and accurate point-of-care detection method is critical for the timely identification and elimination of infected pigs for controlling this disease. In this study, a RT-RAA-CRISPR/Cas13a method was developed for the detection of FMDV serotype O in pigs. Six pairs of RT-RAA primers were designed based on the conserved gene sequence of FMDV serotype O, and the optimal amplification primers and reaction temperatures were screened. The CRISPR-derived RNA (crRNA) was further designed based on the optimal target band sequence and the most efficient crRNA was screened. The results revealed that FMDV-O-F4/R4 was the optimal primer set, and the optimal temperature for the RT-RAA reaction was 37 °C. Moreover, crRNA4 exhibited the strongest detection signal among the six crRNAs. The established RT-RAA-CRISPR/Cas13a method demonstrated high specificity and no cross-reactivity with other common swine pathogens such as Senecavirus A (SVA), porcine reproductive and respiratory virus (PRRSV), porcine epidemic diarrhea virus (PEDV), porcine circovirus type 2 (PCV2), classical swine fever virus (CSFV), and pseudorabies virus (PRV), additionally, it was observed to be highly sensitive, with a detection limit of 19.1 copies/µL. The repeatability of this method was also observed to be good. This method could produce stable fluorescence and exhibited good repeatability when three independent experiments yielded the same results. A validation test using three types of simulated clinical samples (including swab, tissue, and serum samples) revealed a 100% concordance rate. The detection results could be visualized via a fluorescence reader or lateral flow strips (LFSs). Thus, a highly specific and sensitive RT-RAA-CRISPR/Cas13a detection method was developed and is expected to be applied for the rapid detection of FMDV serotype O in situ.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Update: Immunotherapeutic Strategies in HPV-Associated Head and Neck Squamous Cell Carcinoma.","authors":"Fangdi Sun, A Dimitrios Colevas","doi":"10.3390/v17050712","DOIUrl":"10.3390/v17050712","url":null,"abstract":"<p><p>The incidence of human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (OPSCC) has increased substantially over the past three decades, and since 2017, it has been recognized in the AJCC staging system as distinct from its HPV-negative counterpart. The underlying mechanisms of HPV-associated carcinogenesis, tumor microenvironment, and host immune response represent opportunities for therapeutic development. While anti-PD-1 immunotherapy is now part of standard treatment for recurrent or metastatic head and neck squamous cell carcinoma (HNSCC) in general, there are no established immunotherapeutic strategies specifically for HPV-related HNSCC. In this context, multiple emerging approaches are being actively studied-among these are therapeutic vaccines with or without anti-PD-(L)1 adjuvants, peptide-HLA-based immunotherapeutic platforms, and adoptive cell therapies including tumor-infiltrating lymphocytes (TILs), T-cell receptor (TCR) therapy, and chimeric antigen receptor (CAR) T-cell therapy. Beyond further maturation of these novel immunotherapeutic strategies, additional work is needed to delineate the optimal disease state of application (localized versus recurrent/metastatic), as well as in the development of small molecule inhibitors targeting HPV-specific mechanisms of viral oncogenesis.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Papillomatous Lesions and Genetic Diversity of Bovine Papillomavirus from the Amazon Region.","authors":"Fernanda Dos Anjos Souza, Cíntia Daudt, André de Medeiros Costa Lins, Igor Ribeiro Dos Santos, Lorena Yanet Cáceres Tomaya, Agnes de Souza Lima, Eduardo Mitke Brandão Reis, Rafael Augusto Satrapa, David Driemeier, Audrey Bagon, Cláudio Wageck Canal, Felipe Masiero Salvarani, Flavio Roberto Chaves da Silva","doi":"10.3390/v17050719","DOIUrl":"10.3390/v17050719","url":null,"abstract":"<p><p>Bovine papillomaviruses (BPVs) have been widely characterized from cutaneous warts in cattle worldwide. However, there are still limited studies addressing the geographic distribution of viral types and their potential associations with the histopathological characteristics of lesions, particularly in the vast and ecologically diverse Amazon region. This study aimed to histologically and phylogenetically characterize cutaneous papillomatous lesions in cattle from the Vale do Guaporé, located in the Brazilian Western Amazon. A total of 54 wart samples were collected from 44 cattle clinically diagnosed with cutaneous papillomatosis. Histopathological analysis classified 58.33% of cases as fibropapillomas and 39.58% as squamous papillomas. Molecular analysis, based on L1 gene amplification and sequencing, identified the presence of previously reported BPV types (BPV2, 4, 5, 12, 13, and 15), along with a novel BPV14 subtype and three putative new types (PNT). Statistical analysis revealed that BPV2 was significantly associated with fibropapillomas (<i>p</i> = 0.023), whereas BPV13 was linked to cauliflower-like morphological lesions (<i>p</i> = 0.008). These findings enhance the understanding of BPV diversity circulating in cattle from the Amazon region and provide valuable insights into the clinicopathological aspects of bovine cutaneous papillomatosis, which may aid in future epidemiological surveillance and disease control strategies.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}