Viruses-Basel最新文献

Insights into the Landscape of Alphavirus Receptor and Antibody Interactions. 对甲病毒受体和抗体相互作用的见解。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-21 DOI: 10.3390/v17071019

Shishir Poudyal, Abhishek Bandyopadhyay, Richard J Kuhn

{"title":"Insights into the Landscape of Alphavirus Receptor and Antibody Interactions.","authors":"Shishir Poudyal, Abhishek Bandyopadhyay, Richard J Kuhn","doi":"10.3390/v17071019","DOIUrl":"10.3390/v17071019","url":null,"abstract":"Alphaviruses engage a diverse array of attachment factors and receptors during viral entry, resulting in a broad host range and disease spectrum, and thus presenting them as a major global public health concern. The development of effective antivirals against these arboviruses relies on a comprehensive understanding of the molecular interplay between these viruses and host cell factors, as well as the wide range of immune responses that ensue following viral infection. In this review, we present the current understanding of the complex landscape of alphavirus interaction with attachment factors and entry receptors, some of which are characterized structurally, while others are characterized biochemically. Additionally, we provide an overview of the molecular bases of epitope recognition by neutralizing and non-neutralizing antibodies against alphaviruses, and how icosahedral symmetry influences these interactions, such as occupancy and neutralization potency. We further discuss the structural bases of epitope recognition of a few pan-alphavirus antibodies, their potential therapeutic implications, and offer future perspectives on the development of effective therapeutics against clinically relevant alphaviruses.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12298792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses. 用于检测尼帕病毒和亨德拉病毒的即时免疫层析横向流动条带试验的发展。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-21 DOI: 10.3390/v17071021

Jianjun Jia, Wenjun Zhu, Guodong Liu, Sandra Diederich, Bradley Pickering, Logan Banadyga, Ming Yang

{"title":"Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses.","authors":"Jianjun Jia, Wenjun Zhu, Guodong Liu, Sandra Diederich, Bradley Pickering, Logan Banadyga, Ming Yang","doi":"10.3390/v17071021","DOIUrl":"10.3390/v17071021","url":null,"abstract":"Nipah virus (NiV) and Hendra virus (HeV), which both belong to the genus henipavirus, are zoonotic pathogens that cause severe systemic, neurological, and/or respiratory disease in humans and a variety of mammals. Therefore, monitoring viral prevalence in natural reservoirs and rapidly diagnosing cases of henipavirus infection are critical to limiting the spread of these viruses. Current laboratory methods for detecting NiV and HeV include virus isolation, reverse transcription quantitative real-time PCR (RT-qPCR), and antigen detection via an enzyme-linked immunosorbent assay (ELISA), all of which require highly trained personnel and specialized equipment. Here, we describe the development of a point-of-care customized immunochromatographic lateral flow (ILF) assay that uses recombinant human ephrin B2 as a capture ligand on the test line and a NiV-specific monoclonal antibody (mAb) on the conjugate pad to detect NiV and HeV. The ILF assay detects NiV and HeV with a diagnostic specificity of 94.4% and has no cross-reactivity with other viruses. This rapid test may be suitable for field testing and in countries with limited laboratory resources.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12300919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Analysis of the 2022 SARS-CoV-2 Omicron Lineage Replacement Dynamics in Connecticut, US. 美国康涅狄格州2022年SARS-CoV-2基因组序列替换动态的综合分析

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-21 DOI: 10.3390/v17071020

Nicholas F G Chen, Kien Pham, Chrispin Chaguza, Rafael Lopes, Fayette Klaassen, Chaney C Kalinich, Yale Sars-CoV-Genomic Surveillance Initiative, Nicholas Kerantzas, Sameer Pandya, David Ferguson, Wade Schulz, Daniel M Weinberger, Virginia E Pitzer, Joshua L Warren, Nathan D Grubaugh, Anne M Hahn

{"title":"Integrated Analysis of the 2022 SARS-CoV-2 Omicron Lineage Replacement Dynamics in Connecticut, US.","authors":"Nicholas F G Chen, Kien Pham, Chrispin Chaguza, Rafael Lopes, Fayette Klaassen, Chaney C Kalinich, Yale Sars-CoV-Genomic Surveillance Initiative, Nicholas Kerantzas, Sameer Pandya, David Ferguson, Wade Schulz, Daniel M Weinberger, Virginia E Pitzer, Joshua L Warren, Nathan D Grubaugh, Anne M Hahn","doi":"10.3390/v17071020","DOIUrl":"10.3390/v17071020","url":null,"abstract":"In 2022, consecutive sweeps of highly transmissible SARS-CoV-2 Omicron-derived lineages (B.1.1.529*) maintained viral transmission despite extensive antigen exposure from both vaccinations and infections. To better understand Omicron variant emergence in the context of the dynamic fitness landscape of 2022, we aimed to explore putative drivers behind SARS-CoV-2 lineage replacements. Variant fitness is determined through its ability to either outrun previously dominant lineages or more efficiently circumvent host immune responses to previous infections and vaccinations. By analyzing data collected through our local genomic surveillance program from Connecticut, USA, we compared emerging Omicron lineages' growth rates, estimated infections, effective reproductive rates, average viral copy numbers, and likelihood for causing infections in recently vaccinated individuals. We find that newly emerging Omicron lineages outcompeted dominant lineages through a combination of enhanced viral shedding or advanced immune escape depending on the population-level exposure state. This analysis integrates individual-level sequencing data with demographic, vaccination, laboratory, and epidemiological data and provides further insights into host-pathogen dynamics beyond public aggregate data.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12299087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glutamic Acid at Position 343 in PB2 Contributes to the Virulence of H1N1 Swine Influenza Virus in Mice. 小鼠PB2 343位谷氨酸参与H1N1猪流感病毒毒力的研究

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-20 DOI: 10.3390/v17071018

Yanwen Wang, Qiu Zhong, Fei Meng, Zhang Cheng, Yijie Zhang, Zuchen Song, Yali Zhang, Zijian Feng, Yujia Zhai, Yan Chen, Chuanling Qiao, Huanliang Yang

{"title":"Glutamic Acid at Position 343 in PB2 Contributes to the Virulence of H1N1 Swine Influenza Virus in Mice.","authors":"Yanwen Wang, Qiu Zhong, Fei Meng, Zhang Cheng, Yijie Zhang, Zuchen Song, Yali Zhang, Zijian Feng, Yujia Zhai, Yan Chen, Chuanling Qiao, Huanliang Yang","doi":"10.3390/v17071018","DOIUrl":"10.3390/v17071018","url":null,"abstract":"The H1N1 swine influenza viruses CQ91 and CQ445, isolated from pigs in China, exhibited distinct virulence in mice despite sharing similar genomic constellations. CQ91 demonstrated higher pathogenicity (MLD50: 5.4 log10 EID50) and replication efficiency in mice compared to CQ445 (MLD50: 6.6 log10 EID50). Through reverse genetics, we found that the attenuation of CQ445 was due to a single substitution of glutamic acid (E) with lysine (K) at position 343 in the PB2 protein. Introducing the CQ445-PB2 (343K) into CQ91 significantly reduced viral replication and pathogenicity in mice, while replacing CQ445-PB2 with CQ91-PB2 (343E) restored virulence. In vitro studies showed that the K343E mutation impaired viral replication in MDCK and A549 cells and reduced polymerase activity in minigenome assays. Mechanistically, the amino acid at position 343 in the PB2 affects the transcription stage of the viral replication process. Structural modeling indicated that the charge reversal caused by E343K altered local electrostatic interactions without major conformational changes. Phylogenetic analysis revealed that PB2-343E is highly conserved (>99.9%) in human and swine H1/H3 influenza viruses, suggesting that PB2-343E confers an adaptive advantage. This study identifies PB2-343E as a critical determinant of influenza virus pathogenicity in mammals, highlighting its role in host adaptation.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12298360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Bombyx mori-BmNPV Protein Interactions: Study Strategies and Molecular Mechanisms. 桑家蚕- bmnpv蛋白相互作用的调控：研究策略和分子机制。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-20 DOI: 10.3390/v17071017

Dan Guo, Bowen Liu, Mingxing Cui, Heying Qian, Gang Li

{"title":"Regulation of Bombyx mori-BmNPV Protein Interactions: Study Strategies and Molecular Mechanisms.","authors":"Dan Guo, Bowen Liu, Mingxing Cui, Heying Qian, Gang Li","doi":"10.3390/v17071017","DOIUrl":"10.3390/v17071017","url":null,"abstract":"As a pivotal model organism in Lepidoptera research, the silkworm (Bombyx mori) holds significant importance in life science due to its economic value and biotechnological applications. Advancements in proteomics and bioinformatics have enabled substantial progress in characterizing the B. mori proteome. Systematic screening and identification of protein-protein interactions (PPIs) have progressively elucidated the molecular mechanisms governing key biological processes, including viral infection, immune regulation, and growth development. This review comprehensively summarizes traditional PPI detection techniques, such as yeast two-hybrid (Y2H) and immunoprecipitation (IP), alongside emerging methodologies such as mass spectrometry-based interactomics and artificial intelligence (AI)-driven PPI prediction. We critically analyze the strengths, limitations, and technological integration strategies for each approach, highlighting current field challenges. Furthermore, we elaborate on the molecular regulatory networks of Bombyx mori nucleopolyhedrovirus (BmNPV) from multiple perspectives: apoptosis and cell cycle regulation; viral protein invasion and trafficking; non-coding RNA-mediated modulation; metabolic reprogramming; and host immune evasion. These insights reveal the dynamic interplay between viral replication and host defense mechanisms. Collectively, this synthesis aims to provide a robust theoretical foundation and technical guidance for silkworm genetic improvement, infectious disease management, and the advancement of related biotechnological applications.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Novel, Highly Sensitive System for Evaluating Ebola Virus Particle Formation. 一种新型的、高灵敏度的埃博拉病毒颗粒形成评估系统的开发。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-19 DOI: 10.3390/v17071016

Wakako Furuyama, Miako Sakaguchi, Hanako Ariyoshi, Asuka Nanbo

{"title":"Development of a Novel, Highly Sensitive System for Evaluating Ebola Virus Particle Formation.","authors":"Wakako Furuyama, Miako Sakaguchi, Hanako Ariyoshi, Asuka Nanbo","doi":"10.3390/v17071016","DOIUrl":"10.3390/v17071016","url":null,"abstract":"Ebola virus (EBOV) causes severe hemorrhagic fevers in humans, and effective countermeasures remain limited. The EBOV-encoded major matrix protein VP40 is essential for viral assembly, budding, and particle release, making it a promising target for antiviral drug development. However, no approved drugs currently target the viral particle formation process. In this study, we established a simple and highly sensitive screening system to evaluate VP40-mediated virus-like particle (VLP) formation under biosafety level -2 conditions. The system uses the HiBiT luminescence-based reporter fused to VP40, allowing for the detection of VP40 release. Our results demonstrate that the HiBiT sequence fused at the N-terminus [HiBiT-VP40 (N)] retains VP40's ability to form VLPs, supporting its use as a functional reporter. Furthermore, we validated the system by assessing the role of Rab11-dependent trafficking in VP40-mediated budding and by evaluating the effect of nocodazole, a microtubule depolymerizer, on VLP release. This novel screening system provides a convenient and reliable platform for screening potential inhibitors targeting the late stages of EBOV infection, including viral particle formation and release. Additionally, its potential adaptability to other filoviruses suggests wide applicability in the discovery and development of additional novel therapeutic agents.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12298002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Renin-Angiotensin System Modulates SARS-CoV-2 Entry via ACE2 Receptor. 肾素-血管紧张素系统通过ACE2受体调节SARS-CoV-2的进入

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-19 DOI: 10.3390/v17071014

Sophia Gagliardi, Tristan Hotchkin, Hasset Tibebe, Grace Hillmer, Dacia Marquez, Coco Izumi, Jason Chang, Alexander Diggs, Jiro Ezaki, Yuichiro J Suzuki, Taisuke Izumi

{"title":"The Renin-Angiotensin System Modulates SARS-CoV-2 Entry via ACE2 Receptor.","authors":"Sophia Gagliardi, Tristan Hotchkin, Hasset Tibebe, Grace Hillmer, Dacia Marquez, Coco Izumi, Jason Chang, Alexander Diggs, Jiro Ezaki, Yuichiro J Suzuki, Taisuke Izumi","doi":"10.3390/v17071014","DOIUrl":"10.3390/v17071014","url":null,"abstract":"The renin-angiotensin system (RAS) plays a central role in cardiovascular regulation and has gained prominence in the pathogenesis of Coronavirus Disease 2019 (COVID-19) due to the critical function of angiotensin-converting enzyme 2 (ACE2) as the entry receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Angiotensin IV, but not angiotensin II, has recently been reported to enhance the binding between the viral spike protein and ACE2. To investigate the virological significance of this effect, we developed a single-round infection assay using SARS-CoV-2 viral-like particles expressing the spike protein. Our results demonstrate that while angiotensin II does not affect viral infectivity across concentrations ranging from 40 nM to 400 nM, angiotensin IV enhances viral entry at a low concentration but exhibits dose-dependent inhibition at higher concentrations. These findings highlight the unique dual role of angiotensin IV in modulating SARS-CoV-2 entry. In silico molecular docking simulations indicate that angiotensin IV was predicted to associate with the S1 domain near the receptor-binding domain in the open spike conformation. Given that reported plasma concentrations of angiotensin IV range widely from 17 pM to 81 nM, these levels may be sufficient to promote, rather than inhibit, SARS-CoV-2 infection. This study identifies a novel link between RAS-derived peptides and SARS-CoV-2 infectivity, offering new insights into COVID-19 pathophysiology and informing potential therapeutic strategies.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12299366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rift Valley Fever Outbreak Investigation Associated with a Dairy Farm Abortion Storm, Mbarara District, Western Uganda, 2023. 裂谷热爆发调查与奶牛场流产风暴有关，乌干达西部姆巴拉拉地区，2023年。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-19 DOI: 10.3390/v17071015

Luke Nyakarahuka, Shannon Whitmer, Sophia Mulei, Joanita Mutesi, Jimmy Baluku, Jackson Kyondo, Amy Whitesell, Carson Telford, Alex Tumusiime, Calvin Richie Torach, Dianah Namanya, Mariam Nambuya, Dominic Muhereza, Zainah Kabami, Annet Nankya, David Muwanguzi, Francis Mugabi, Nelson Wandera, Rose Muhindo, Joel M Montgomery, Julius J Lutwama, Stephen Karabyo Balinandi, John D Klena, Trevor R Shoemaker

{"title":"Rift Valley Fever Outbreak Investigation Associated with a Dairy Farm Abortion Storm, Mbarara District, Western Uganda, 2023.","authors":"Luke Nyakarahuka, Shannon Whitmer, Sophia Mulei, Joanita Mutesi, Jimmy Baluku, Jackson Kyondo, Amy Whitesell, Carson Telford, Alex Tumusiime, Calvin Richie Torach, Dianah Namanya, Mariam Nambuya, Dominic Muhereza, Zainah Kabami, Annet Nankya, David Muwanguzi, Francis Mugabi, Nelson Wandera, Rose Muhindo, Joel M Montgomery, Julius J Lutwama, Stephen Karabyo Balinandi, John D Klena, Trevor R Shoemaker","doi":"10.3390/v17071015","DOIUrl":"10.3390/v17071015","url":null,"abstract":"In Africa, Rift Valley Fever poses a substantial risk to animal health, and human cases occur after contact with infected animals or their tissues. RVF has re-emerged in Uganda after nearly five decades, with multiple outbreaks recorded since 2016. We investigated a unique RVF outbreak associated with an animal abortion storm of 30 events and human cases on a dairy farm in Mbarara District, Western Uganda, in February 2023. Genomic analysis was performed, comparing animal and human RVF viruses (RVFV) circulating in the region. A cluster of thirteen human RVF cases and nine PCR-positive animals could directly be linked with the abortion storm. Overall, during the year 2023, we confirmed 61 human RVFV cases across Uganda, 88.5% of which were reported to have had direct contact with livestock, and a high case fatality rate of 31%. We recommend implementing extensive health education programs in affected communities and using sustainable mosquito control strategies to limit transmission in livestock, coupled with initiating animal vaccination trials in Uganda.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12298680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the Mouse Infection Model for Echovirus 18. 小鼠Echovirus 18感染模型的研究。

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-18 DOI: 10.3390/v17071011

Lei Xiang, Linlin Zhai, Guanyong Ou, Wei Zhao, Yang Yang, Chenguang Shen

引用次数: 0

Overexpression of Interleukin-17 Modulates Responses to Marek's Disease Virus Infection and Tumor Formation in Chickens. 白介素-17过表达调节鸡对马立克病病毒感染和肿瘤形成的反应

IF 3.5 3区医学

Viruses-Basel Pub Date : 2025-07-18 DOI: 10.3390/v17071009

Nitish Boodhoo, Katherine Blake, Fatemeh Fazel, Janan Shoja Doost, Shayan Sharif

{"title":"Overexpression of Interleukin-17 Modulates Responses to Marek's Disease Virus Infection and Tumor Formation in Chickens.","authors":"Nitish Boodhoo, Katherine Blake, Fatemeh Fazel, Janan Shoja Doost, Shayan Sharif","doi":"10.3390/v17071009","DOIUrl":"10.3390/v17071009","url":null,"abstract":"Marek's Disease Virus (MDV) is a highly contagious pathogen in chickens, resulting in immunosuppression and T-cell lymphomas. Understanding the role of host cytokines in MDV pathogenesis is crucial for developing effective interventions. This study investigated the in vivo effects of overexpressing avian interleukin-17 (IL-17) in Marek's disease virus infection model and its impact on T-cell populations. We utilized a recombinant pCDNA3.1 plasmid that expresses IL-17 at days 4 and 10 post-MDV infection in chickens. Our findings demonstrate that IL-17 overexpression significantly enhanced MDV replication. However, treatment with the plasmid expressing IL-17 led to a reduction in MD disease severity. Additionally, IL-17 treatment markedly altered the frequency of CD4+ and CD8α+ αβ T-cells. Specifically, at 21-dpi, there was an increase in CD3+ CD8α+ αβ T cells and a decrease in CD3+ CD4+ αβ T-cells within the spleen of chickens treated with the plasmid expressing IL-17. These modulatory effects suggest a possible mechanism by which IL-17 facilitates immune system cell activation and enhances viral persistence. This study underscores the pivotal role of IL-17 in MDV infection dynamics and offers.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12298531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0