Biomolecular NMR Assignments最新文献_第2页

Resonance assignment of the intrinsically disordered actin-binding region of Drebrin 德雷布林内在无序动作蛋白结合区的共振分配。

IF 0.6 4区生物学

Biomolecular NMR Assignments Pub Date : 2025-06-14 DOI: 10.1007/s12104-025-10239-0

Soma Varga, Julie Maibøll Kaasen, Zoltán Gáspári, Bálint Ferenc Péterfia, Frans A. A. Mulder

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引用次数: 0

Backbone and sidechain 1H, 15N and 13C resonance assignments of a multidrug efflux membrane protein using solution and solid-state NMR 多药外排膜蛋白的主链和侧链1H， 15N和13C共振分配使用溶液和固态核磁共振。

IF 0.6 4区生物学

Biomolecular NMR Assignments Pub Date : 2025-05-27 DOI: 10.1007/s12104-025-10238-1

Benjamin D. Harding, Ashley Hiett, Marco Tonelli, Songlin Wang, Chad M. Rienstra, Katherine A. Henzler-Wildman

引用次数: 0

Correction: Backbone resonance assignments of the CPEB3 [101–200] and CPEB3 [294–410] 修正：CPEB3[101-200]和CPEB3[294-410]的主干共振分配。

IF 0.6 4区生物学

Biomolecular NMR Assignments Pub Date : 2025-05-09 DOI: 10.1007/s12104-025-10237-2

Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase

引用次数: 0

NMR 1H, 13C, and 15N resonance assignments of the oncogenic Q61R variant of human NRAS in the active, GTP-bound conformation 人类NRAS活性gtp结合构象的致癌Q61R变体的NMR 1H， 13C和15N共振分配。

IF 0.6 4区生物学

Biomolecular NMR Assignments Pub Date : 2025-05-02 DOI: 10.1007/s12104-025-10236-3

Alok K. Sharma, Marco Tonelli, Marcin Dyba, William K. Gillette, Dominic Esposito, Dwight V. Nissley, Frank McCormick, Anna E. Maciag

{"title":"NMR 1H, 13C, and 15N resonance assignments of the oncogenic Q61R variant of human NRAS in the active, GTP-bound conformation","authors":"Alok K. Sharma, Marco Tonelli, Marcin Dyba, William K. Gillette, Dominic Esposito, Dwight V. Nissley, Frank McCormick, Anna E. Maciag","doi":"10.1007/s12104-025-10236-3","DOIUrl":"10.1007/s12104-025-10236-3","url":null,"abstract":"<div>NRASQ61R is a frequent mutation in melanoma. Hydrolysis of GTP by NRASQ61R is reported to be much slower than other KRAS and NRAS mutants. Recent structural biology efforts for KRAS and NRAS proteins have been limited to X-ray crystallography and therefore lack insight into the structure and dynamics of these proteins in solution. Here we report the 1HN, 15N, and 13C backbone and sidechain resonance assignments of the G-domain of oncogenic NRASQ61R-GTP (MW 19.3 kDa; aa 1–169) using heteronuclear, multidimensional NMR spectroscopy. NRASQ61R-GTP is a conformationally stable protein in solution. The 1H–15N correlation cross-peaks in a 2D 1H–15N HSQC spectrum collected after 48 h at 298 K remained intact and only minimal signs of peak-broadening were noted for select residues. High resolution NMR allowed unambiguous assignments of the 1H–15N correlation cross-peaks for all aa residues, except Y40, in addition to a significantly large number of aliphatic and aromatic sidechain resonances. NRASQ61R-GTP exhibits canonical secondary structural elements in the 5 (five) α-helices, 6 (six) β-strands, and associated loop regions as predicted in TALOS-N and CSI. Order parameter (RCI-S2) values predicted by TALOS-N indicate that the NRASQ61R-GTP switch (SW) regions and overall backbone are less flexible than observed in KRAS4b-GTP. The SW region rigidification was validated in heteronuclear NOE measurements. 31P NMR experiments indicate that the G-domain of NRASQ61R-GTP is in a predominant state 2 (active) conformation.</div>","PeriodicalId":492,"journal":{"name":"Biomolecular NMR Assignments","volume":"19 1","pages":"195 - 203"},"PeriodicalIF":0.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

1H, 13C and 15N resonance assignments for the acetyltransferase domain of the kinetoplastid kinetochore protein KKT23 from Trypanosoma brucei 布鲁氏锥虫着丝粒蛋白KKT23乙酰转移酶结构域的1H， 13C和15N共振分配。

IF 0.6 4区生物学

Biomolecular NMR Assignments Pub Date : 2025-05-02 DOI: 10.1007/s12104-025-10235-4

Patryk Ludzia, Charlotte Nugent, Bungo Akiyoshi, Christina Redfield

引用次数: 0

1H, 13C, and 15N resonance assignment of the 5’SL-bound La domain of the human La-related protein 6 人La相关蛋白5' l结合的La结构域的1H， 13C和15N共振分配

IF 0.6 4区生物学

Biomolecular NMR Assignments Pub Date : 2025-04-30 DOI: 10.1007/s12104-025-10232-7

Blaine H. Gordon, Robert Silvers

引用次数: 0

13C and 15N resonance assignments of the DNA binding domain of interferon regulatory factor-3 干扰素调节因子-3 DNA结合域的13C和15N共振分配。

IF 0.6 4区生物学

Biomolecular NMR Assignments Pub Date : 2025-04-28 DOI: 10.1007/s12104-025-10234-5

Nitin Dhaka, Sunirmala Sahoo, Biswajit Samal, Sulakshana P. Mukherjee

引用次数: 0

NMR chemical shift assignment of the IMLV methyl groups of a di-domain of the Tomaymycin non-ribosomal peptide synthetase 托马霉素非核糖体肽合成酶双结构域的IMLV甲基核磁共振化学移位分配。

IF 0.6 4区生物学

Biomolecular NMR Assignments Pub Date : 2025-04-25 DOI: 10.1007/s12104-025-10231-8

John P. Kirkpatrick, Megha N. Karanth, Teresa Carlomagno

{"title":"NMR chemical shift assignment of the IMLV methyl groups of a di-domain of the Tomaymycin non-ribosomal peptide synthetase","authors":"John P. Kirkpatrick, Megha N. Karanth, Teresa Carlomagno","doi":"10.1007/s12104-025-10231-8","DOIUrl":"10.1007/s12104-025-10231-8","url":null,"abstract":"<div>Non-ribosomal peptide synthetases (NRPSs) are macromolecular enzymatic complexes responsible for the biosynthesis of an array of microbial natural products, many of which have important applications for human health. The nature of the NRPS machinery, which has been likened to an assembly line, should be amenable to bio-engineering efforts directed towards efficient synthesis of novel and tailored molecules. However, the success of such endeavours depends on a detailed understanding of the mechanistic principles governing the various steps in the peptide assembly-line. Here, we report the near-complete assignment of the Ile, Met, Leu and Val methyl-groups of the 59-kDa adaptor-condensation di-domain (BN-BC) from the Tomaymycin NRPS. These assignments will provide the foundation for future NMR studies of the complex dynamic behaviour of the condensation domain both in isolation and in the context of the enzymatic cycle, which will themselves form the basis for developing a complete mechanistic description of the central condensation reaction in this prototypical NRPS.</div>","PeriodicalId":492,"journal":{"name":"Biomolecular NMR Assignments","volume":"19 1","pages":"153 - 164"},"PeriodicalIF":0.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

1H, 13C and 15N backbone resonance assignment of the catalytic domain of human PTPN22 人PTPN22催化结构域的1H， 13C和15N骨架共振分配。

IF 0.6 4区生物学

Biomolecular NMR Assignments Pub Date : 2025-04-25 DOI: 10.1007/s12104-025-10233-6

Bryan T. Martin, Michael W. Clarkson, Ping Wu, Paola Di Lello

引用次数: 0

Chemical shift assignments of the rib domain in a cell surface protein from Limosilactobacillus reuteri 罗伊氏芽孢杆菌细胞表面蛋白肋结构域的化学位移分配。

IF 0.6 4区生物学

Biomolecular NMR Assignments Pub Date : 2025-04-07 DOI: 10.1007/s12104-025-10228-3

Yi Xue, Xue Kang

引用次数: 0