多药外排膜蛋白的主链和侧链1H， 15N和13C共振分配使用溶液和固态核磁共振。

IF 0.6 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2025-05-27 DOI:10.1007/s12104-025-10238-1

Benjamin D. Harding, Ashley Hiett, Marco Tonelli, Songlin Wang, Chad M. Rienstra, Katherine A. Henzler-Wildman

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引用次数: 0

摘要

EmrE是一种细菌膜嵌入的多药物转运体，其功能是不对称的同型二聚体。EmrE与抗生素耐药性有关，但现在已知根据小分子底物的特性赋予耐药性或易感性。在这里，我们报告了S64V-EmrE在pH 5.8下的溶液和固体核磁共振分配，低于关键残留物E14和H110的pKa。这包括单体溶液核磁共振实验中主链、甲基（异亮氨酸、亮氨酸、缬氨酸、苏氨酸和丙氨酸）的1H、15N和13C共振配位，以及脂质体中固态核磁共振13C检测实验中主链和侧链的配位。

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Backbone and sidechain 1H, 15N and 13C resonance assignments of a multidrug efflux membrane protein using solution and solid-state NMR

EmrE is a bacterial membrane-embedded multidrug transporter that functions as an asymmetric homodimer. EmrE is implicated in antibiotic resistance but is now known to confer either resistance or susceptibility depending on the identity of the small molecule substrate. Here, we report both solution- and solid-state NMR assignments of S64V-EmrE at pH 5.8, below the pKa of critical residues E14 and H110. This includes ¹H, ¹⁵N, and ¹³C resonance assignments of the backbone, methyl groups (isoleucine, leucine, valine, threonine and alanine) from solution NMR experiments in bicelles, and backbone and side-chain assignments from solid-state NMR ¹³C-detected experiments in liposomes.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.