干扰素调节因子-3 DNA结合域的13C和15N共振分配。

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2025-04-28 DOI:10.1007/s12104-025-10234-5

Nitin Dhaka, Sunirmala Sahoo, Biswajit Samal, Sulakshana P Mukherjee

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引用次数: 0

摘要

干扰素调节因子（IRF）家族转录因子是众所周知的抗病毒活性在脊椎动物。IRF家族包括9个成员（IRF1-9），它们具有诱导干扰素β (IFNβ)启动子的能力。IRF3和IRF7是参与生产I型和III型IFN的关键家族成员。IRF3和IRF7都包含一个DNA结合域（DBD），它与靶基因启动子上的同源干扰素响应元件（IRE）结合。在这里，我们报道了IRF家族的IRF3亚基的DBD结构域的几乎完整的主链和部分侧链共振分配。利用主链化学位移预测的二级结构与晶体结构的预测结果基本一致，TALOS-N预测的二级结构显示出略长的β-链。

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¹³C and ¹⁵N resonance assignments of the DNA binding domain of interferon regulatory factor-3.

The Interferon Regulatory Factor (IRF) family of transcription factors is well known for its anti-viral activity in vertebrates. The IRF family comprises nine members (IRF1-9) which have the ability to induce the Interferon beta (IFNβ) promotor. The IRF3 and IRF7 are the key family members involved in the production of type I and type III IFN. IRF3 and IRF7 both comprise of a DNA binding domain (DBD) which binds to its cognate interferon responsive element (IRE) on its target gene promoters. Here, we report near complete backbone and partial side-chain resonance assignments of the DBD domain of the IRF3 subunit of the IRF family. The predicted secondary structure using the backbone chemical shifts largely conforms with that obtained from the crystal structure, with the TALOS-N predicted secondary structures showing slightly elongated β-strands.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.