¹H, ¹³C and ¹⁵N resonance assignments for the acetyltransferase domain of the kinetoplastid kinetochore protein KKT23 from Trypanosoma brucei.

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2025-05-02 DOI:10.1007/s12104-025-10235-4

Patryk Ludzia, Charlotte Nugent, Bungo Akiyoshi, Christina Redfield

引用次数: 0

Abstract

KKT23 is a kinetoplastid-specific kinetochore protein that has a C-terminal GCN5-related histone acetyltransferase domain that acetylates the C-terminal tail of histone H2A. Here, we present the ¹H, ¹³C and ¹⁵N resonance assignments for the C-terminal region of KKT23 (KKT23^125-348) from Trypanosoma brucei in complex with known cofactors for acetyltransferases, acetyl coenzyme A and coenzyme A. These assignments provide the starting point for detailed investigation of the structure, dynamics and interactions of KKT23 in solution.

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布鲁氏锥虫着丝粒蛋白KKT23乙酰转移酶结构域的1H， 13C和15N共振分配。

KKT23是一种着丝质体特异性的着丝粒蛋白，具有c端gcn5相关的组蛋白乙酰转移酶结构域，可使组蛋白H2A的c端尾部乙酰化。在这里，我们给出了来自布氏锥虫的KKT23 （KKT23125-348）的c端区域与已知的乙酰转移酶、乙酰辅酶A和辅酶A的辅因子的1H、13C和15N共振赋值，这些赋值为详细研究KKT23在溶液中的结构、动力学和相互作用提供了起点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.

1H, 13C and 15N resonance assignments for the acetyltransferase domain of the kinetoplastid kinetochore protein KKT23 from Trypanosoma brucei.

Abstract

¹H, ¹³C and ¹⁵N resonance assignments for the acetyltransferase domain of the kinetoplastid kinetochore protein KKT23 from Trypanosoma brucei.