PLoS Biology最新文献_第4页

Free long-chain fatty acids trigger early postembryonic development in starved Caenorhabditis elegans by suppressing mTORC1. 游离长链脂肪酸通过抑制 mTORC1 触发饥饿的秀丽隐杆线虫胚后早期发育。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2024-10-22 eCollection Date: 2024-10-01 DOI: 10.1371/journal.pbio.3002841

Meiyu Ruan, Fan Xu, Na Li, Jing Yu, Fukang Teng, Jiawei Tang, Cheng Huang, Huanhu Zhu

{"title":"Free long-chain fatty acids trigger early postembryonic development in starved Caenorhabditis elegans by suppressing mTORC1.","authors":"Meiyu Ruan, Fan Xu, Na Li, Jing Yu, Fukang Teng, Jiawei Tang, Cheng Huang, Huanhu Zhu","doi":"10.1371/journal.pbio.3002841","DOIUrl":"10.1371/journal.pbio.3002841","url":null,"abstract":"Postembryonic development of animals has long been considered an internally predetermined program, while macronutrients were believed to be essential solely for providing biomatters and energy to support this process. However, in this study, by using a nematode Caenorhabditis elegans (abbreviated as C. elegans hereafter) model, we surprisingly discovered that dietary supplementation of palmitic acid alone, rather than other abundant essential nutrients such as glucose or amino acid mixture, was sufficient to initiate early postembryonic development even under complete macronutrient deprivation. Such a development was evidenced by changes in morphology, cellular markers in multiple tissues, behaviors, and the global transcription pattern and it occurred earlier than the well-known early L1 nutrient checkpoint. Mechanistically, palmitic acid did not function as a biomatter/energy provider, but rather as a ligand to activate the nuclear hormone receptor NHR-49/80, leading to the production of an unknown peroxisome-derived secretive hormone in the intestine. This hormonal signal was received by chemosensory neurons in the head, regulating the insulin-like neuropeptide secretion and its downstream nuclear receptor to orchestrate global development. Additionally, the nutrient-sensing hub mTORC1 played a negative role in this process. In conclusion, our data indicate that free fatty acids act as a primary nutrient signal to launch the early development in C. elegans, which suggests that specific nutrients, rather than the internal genetic program, serve as the first impetus for postembryonic development.","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142511126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding reveals the neural representation of perceived and imagined musical sounds. 解码揭示了感知和想象的音乐声音的神经表征。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2024-10-21 eCollection Date: 2024-10-01 DOI: 10.1371/journal.pbio.3002858

David R Quiroga-Martinez, Gemma Fernández Rubio, Leonardo Bonetti, Kriti G Achyutuni, Athina Tzovara, Robert T Knight, Peter Vuust

引用次数: 0

Activation of plant immunity through conversion of a helper NLR homodimer into a resistosome. 通过将辅助 NLR 同源二聚体转化为抗原体激活植物免疫。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2024-10-18 eCollection Date: 2024-10-01 DOI: 10.1371/journal.pbio.3002868

Muniyandi Selvaraj, AmirAli Toghani, Hsuan Pai, Yu Sugihara, Jiorgos Kourelis, Enoch Lok Him Yuen, Tarhan Ibrahim, He Zhao, Rongrong Xie, Abbas Maqbool, Juan Carlos De la Concepcion, Mark J Banfield, Lida Derevnina, Benjamin Petre, David M Lawson, Tolga O Bozkurt, Chih-Hang Wu, Sophien Kamoun, Mauricio P Contreras

{"title":"Activation of plant immunity through conversion of a helper NLR homodimer into a resistosome.","authors":"Muniyandi Selvaraj, AmirAli Toghani, Hsuan Pai, Yu Sugihara, Jiorgos Kourelis, Enoch Lok Him Yuen, Tarhan Ibrahim, He Zhao, Rongrong Xie, Abbas Maqbool, Juan Carlos De la Concepcion, Mark J Banfield, Lida Derevnina, Benjamin Petre, David M Lawson, Tolga O Bozkurt, Chih-Hang Wu, Sophien Kamoun, Mauricio P Contreras","doi":"10.1371/journal.pbio.3002868","DOIUrl":"10.1371/journal.pbio.3002868","url":null,"abstract":"Nucleotide-binding domain and leucine-rich repeat (NLR) proteins can engage in complex interactions to detect pathogens and execute a robust immune response via downstream helper NLRs. However, the biochemical mechanisms of helper NLR activation by upstream sensor NLRs remain poorly understood. Here, we show that the coiled-coil helper NLR NRC2 from Nicotiana benthamiana accumulates in vivo as a homodimer that converts into a higher-order oligomer upon activation by its upstream virus disease resistance protein Rx. The cryo-EM structure of NbNRC2 in its resting state revealed intermolecular interactions that mediate homodimer formation and contribute to immune receptor autoinhibition. These dimerization interfaces have diverged between paralogous NRC proteins to insulate critical network nodes and enable redundant immune pathways, possibly to minimise undesired cross-activation and evade pathogen suppression of immunity. Our results expand the molecular mechanisms of NLR activation pointing to transition from homodimers to higher-order oligomeric resistosomes.","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142478607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structures of the mycobacterial MmpL4 and MmpL5 transporters provide insights into their role in siderophore export and iron acquisition. 霉菌 MmpL4 和 MmpL5 转运体的结构揭示了它们在苷酸输出和铁获取中的作用。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2024-10-18 eCollection Date: 2024-10-01 DOI: 10.1371/journal.pbio.3002874

Rakesh Maharjan, Zhemin Zhang, Philip A Klenotic, William D Gregor, Marios L Tringides, Meng Cui, Georgiana E Purdy, Edward W Yu

{"title":"Structures of the mycobacterial MmpL4 and MmpL5 transporters provide insights into their role in siderophore export and iron acquisition.","authors":"Rakesh Maharjan, Zhemin Zhang, Philip A Klenotic, William D Gregor, Marios L Tringides, Meng Cui, Georgiana E Purdy, Edward W Yu","doi":"10.1371/journal.pbio.3002874","DOIUrl":"10.1371/journal.pbio.3002874","url":null,"abstract":"The Mycobacterium tuberculosis (Mtb) pathogen, the causative agent of the airborne infection tuberculosis (TB), harbors a number of mycobacterial membrane protein large (MmpL) transporters. These membrane proteins can be separated into 2 distinct subclasses, where they perform important functional roles, and thus, are considered potential drug targets to combat TB. Previously, we reported both X-ray and cryo-EM structures of the MmpL3 transporter, providing high-resolution structural information for this subclass of the MmpL proteins. Currently, there is no structural information available for the subclass associated with MmpL4 and MmpL5, transporters that play a critical role in iron homeostasis of the bacterium. Here, we report cryo-EM structures of the M. smegmatis MmpL4 and MmpL5 transporters to resolutions of 2.95 Å and 3.00 Å, respectively. These structures allow us to propose a plausible pathway for siderophore translocation via these 2 transporters, an essential step for iron acquisition that enables the survival and replication of the mycobacterium.","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142478513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatin remodeller Chd7 is developmentally regulated in the neural crest by tissue-specific transcription factors. 染色质重塑因子 Chd7 在神经嵴中受组织特异性转录因子的发育调控。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2024-10-17 eCollection Date: 2024-10-01 DOI: 10.1371/journal.pbio.3002786

Ruth M Williams, Guneş Taylor, Irving T C Ling, Ivan Candido-Ferreira, Daniel M Fountain, Sarah Mayes, Perihan Seda Ateş-Kalkan, Julianna O Haug, Andrew J Price, Sean A McKinney, Yavor K Bozhilovh, Richard C V Tyser, Shankar Srinivas, Jim R Hughes, Tatjana Sauka-Spengler

{"title":"Chromatin remodeller Chd7 is developmentally regulated in the neural crest by tissue-specific transcription factors.","authors":"Ruth M Williams, Guneş Taylor, Irving T C Ling, Ivan Candido-Ferreira, Daniel M Fountain, Sarah Mayes, Perihan Seda Ateş-Kalkan, Julianna O Haug, Andrew J Price, Sean A McKinney, Yavor K Bozhilovh, Richard C V Tyser, Shankar Srinivas, Jim R Hughes, Tatjana Sauka-Spengler","doi":"10.1371/journal.pbio.3002786","DOIUrl":"10.1371/journal.pbio.3002786","url":null,"abstract":"Neurocristopathies such as CHARGE syndrome result from aberrant neural crest development. A large proportion of CHARGE cases are attributed to pathogenic variants in the gene encoding CHD7, chromodomain helicase DNA binding protein 7, which remodels chromatin. While the role for CHD7 in neural crest development is well documented, how this factor is specifically up-regulated in neural crest cells is not understood. Here, we use epigenomic profiling of chick and human neural crest to identify a cohort of enhancers regulating Chd7 expression in neural crest cells and other tissues. We functionally validate upstream transcription factor binding at candidate enhancers, revealing novel epistatic relationships between neural crest master regulators and Chd7, showing tissue-specific regulation of a globally acting chromatin remodeller. Furthermore, we find conserved enhancer features in human embryonic epigenomic data and validate the activity of the human equivalent CHD7 enhancers in the chick embryo. Our findings embed Chd7 in the neural crest gene regulatory network and offer potentially clinically relevant elements for interpreting CHARGE syndrome cases without causative allocation.","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142478508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mice employ a bait-and-switch escape mechanism to de-escalate social conflict. 小鼠采用诱饵-开关逃脱机制来缓和社会冲突。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2024-10-15 eCollection Date: 2024-10-01 DOI: 10.1371/journal.pbio.3002496

Rachel S Clein, Megan R Warren, Joshua P Neunuebel

{"title":"Mice employ a bait-and-switch escape mechanism to de-escalate social conflict.","authors":"Rachel S Clein, Megan R Warren, Joshua P Neunuebel","doi":"10.1371/journal.pbio.3002496","DOIUrl":"10.1371/journal.pbio.3002496","url":null,"abstract":"Intraspecies aggression has profound ecological and evolutionary consequences, as recipients can suffer injuries, decreases in fitness, and become outcasts from social groups. Although animals implement diverse strategies to avoid hostile confrontations, the extent to which social influences affect escape tactics is unclear. Here, we used computational and machine-learning approaches to analyze complex behavioral interactions as mixed-sex groups of mice, Mus musculus, freely interacted. Mice displayed a rich repertoire of behaviors marked by changes in behavioral state, aggressive encounters, and mixed-sex interactions. A distinctive behavioral sequence consistently occurred after aggressive encounters, where males in submissive states quickly approached and transiently interacted with females immediately before the aggressor engaged with the same female. The behavioral sequences were also associated with substantially fewer physical altercations. Furthermore, the male's behavioral state could be predicted by distinct features of the behavioral sequence, such as kinematics and the latency to and duration of male-female interactions. More broadly, our work revealed an ethologically relevant escape strategy influenced by the presence of females that may serve as a mechanism for de-escalating social conflict and preventing consequential reductions in fitness.","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142478512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The human olfactory bulb communicates perceived odor valence to the piriform cortex in the gamma band and receives a refined representation back in the beta band. 人的嗅球通过伽马波段将感知到的气味价位传递给梨状皮层，并通过贝塔波段接收回馈的精细表征。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2024-10-14 eCollection Date: 2024-10-01 DOI: 10.1371/journal.pbio.3002849

Frans Nordén, Behzad Iravani, Martin Schaefer, Anja L Winter, Mikael Lundqvist, Artin Arshamian, Johan N Lundström

{"title":"The human olfactory bulb communicates perceived odor valence to the piriform cortex in the gamma band and receives a refined representation back in the beta band.","authors":"Frans Nordén, Behzad Iravani, Martin Schaefer, Anja L Winter, Mikael Lundqvist, Artin Arshamian, Johan N Lundström","doi":"10.1371/journal.pbio.3002849","DOIUrl":"10.1371/journal.pbio.3002849","url":null,"abstract":"A core function of the olfactory system is to determine the valence of odors. In humans, central processing of odor valence perception has been shown to take form already within the olfactory bulb (OB), but the neural mechanisms by which this important information is communicated to, and from, the olfactory cortex (piriform cortex, PC) are not known. To assess communication between the 2 nodes, we simultaneously measured odor-dependent neural activity in the OB and PC from human participants while obtaining trial-by-trial valence ratings. By doing so, we could determine when subjective valence information was communicated, what kind of information was transferred, and how the information was transferred (i.e., in which frequency band). Support vector machine (SVM) learning was used on the coherence spectrum and frequency-resolved Granger causality to identify valence-dependent differences in functional and effective connectivity between the OB and PC. We found that the OB communicates subjective odor valence to the PC in the gamma band shortly after odor onset, while the PC subsequently feeds broader valence-related information back to the OB in the beta band. Decoding accuracy was better for negative than positive valence, suggesting a focus on negative valence. Critically, we replicated these findings in an independent data set using additional odors across a larger perceived valence range. Combined, these results demonstrate that the OB and PC communicate levels of subjective odor pleasantness across multiple frequencies, at specific time points, in a direction-dependent pattern in accordance with a two-stage model of odor processing.","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11501019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142478514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduction of endocytosis and EGFR signaling is associated with the switch from isolated to clustered apoptosis during epithelial tissue remodeling in Drosophila. 果蝇上皮组织重塑过程中，内吞和表皮生长因子受体信号转导的减少与细胞凋亡从孤立凋亡到集群凋亡的转变有关。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2024-10-14 eCollection Date: 2024-10-01 DOI: 10.1371/journal.pbio.3002823

Kevin Yuswan, Xiaofei Sun, Erina Kuranaga, Daiki Umetsu

{"title":"Reduction of endocytosis and EGFR signaling is associated with the switch from isolated to clustered apoptosis during epithelial tissue remodeling in Drosophila.","authors":"Kevin Yuswan, Xiaofei Sun, Erina Kuranaga, Daiki Umetsu","doi":"10.1371/journal.pbio.3002823","DOIUrl":"https://doi.org/10.1371/journal.pbio.3002823","url":null,"abstract":"Epithelial tissues undergo cell turnover both during development and for homeostatic maintenance. Removal of cells is coordinated with the increase in number of newly dividing cells to maintain barrier function of the tissue. In Drosophila metamorphosis, larval epidermal cells (LECs) are replaced by adult precursor cells called histoblasts. Removal of LECs must counterbalance the exponentially increasing adult histoblasts. Previous work showed that the LEC removal accelerates as endocytic activity decreases throughout all LECs. Here, we show that the acceleration is accompanied by a mode switching from isolated single-cell apoptosis to clustered ones induced by the endocytic activity reduction. We identify the epidermal growth factor receptor (EGFR) pathway via extracellular-signal regulated kinase (ERK) activity as the main components downstream of endocytic activity in LECs. The reduced ERK activity, caused by the decrease in endocytic activity, is responsible for the apoptotic mode switching. Initially, ERK is transiently activated in normal LECs surrounding a single apoptotic LEC in a ligand-dependent manner, preventing clustered cell death. Following the reduction of endocytic activity, LEC apoptosis events do not provoke these transient ERK up-regulations, resulting in the acceleration of the cell elimination rate by frequent clustered apoptosis. These findings contrasted with the common perspective that clustered apoptosis is disadvantageous. Instead, switching to clustered apoptosis is required to accommodate the growth of neighboring tissues.","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11472926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142485879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Widespread regulation of the maternal transcriptome by Nanos in Drosophila. 果蝇的母体转录组受到 Nanos 的广泛调控。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2024-10-14 eCollection Date: 2024-10-01 DOI: 10.1371/journal.pbio.3002840

Mohammad Marhabaie, Tammy H Wharton, Sung Yun Kim, Robin P Wharton

{"title":"Widespread regulation of the maternal transcriptome by Nanos in Drosophila.","authors":"Mohammad Marhabaie, Tammy H Wharton, Sung Yun Kim, Robin P Wharton","doi":"10.1371/journal.pbio.3002840","DOIUrl":"10.1371/journal.pbio.3002840","url":null,"abstract":"The translational repressor Nanos (Nos) regulates a single target, maternal hunchback (hb) mRNA, to govern abdominal segmentation in the early Drosophila embryo. Nos is recruited to sites in the 3' UTR of hb mRNA in collaboration with the sequence-specific RNA-binding protein Pumilio (Pum); on its own, Nos has no binding specificity. Nos is expressed at other stages of development, but very few mRNA targets that might mediate its action at these stages have been described. Nor has it been clear whether Nos is targeted to other mRNAs in concert with Pum or via other mechanisms. In this report, we identify mRNAs targeted by Nos via 2 approaches. First, we identify mRNAs depleted upon expression of a chimera bearing Nos fused to the nonsense mediated decay (NMD) factor Upf1. We find that, in addition to hb, Upf1-Nos depletes approximately 2,600 mRNAs from the maternal transcriptome in early embryos. Virtually all of these appear to be targeted in a canonical, hb-like manner in concert with Pum. In a second, more conventional approach, we identify mRNAs that are stabilized during the maternal zygotic transition (MZT) in embryos from nos- females. Most (86%) of the 1,185 mRNAs regulated by Nos are also targeted by Upf1-Nos, validating use of the chimera. Previous work has shown that 60% of the maternal transcriptome is degraded in early embryos. We find that maternal mRNAs targeted by Upf1-Nos are hypoadenylated and inefficiently translated at the ovary-embryo transition; they are subsequently degraded in the early embryo, accounting for 59% of all destabilized maternal mRNAs. We suggest that the late ovarian burst of Nos represses a large fraction of the maternal transcriptome, priming it for later degradation by other factors in the embryo.","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11501031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142478515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional networks of inhibitory neurons orchestrate synchrony in the hippocampus. 抑制性神经元的功能网络协调了海马的同步性。

IF 9.8 1区生物学

PLoS Biology Pub Date : 2024-10-14 eCollection Date: 2024-10-01 DOI: 10.1371/journal.pbio.3002837

Marco Bocchio, Artem Vorobyev, Sadra Sadeh, Sophie Brustlein, Robin Dard, Susanne Reichinnek, Valentina Emiliani, Agnes Baude, Claudia Clopath, Rosa Cossart

{"title":"Functional networks of inhibitory neurons orchestrate synchrony in the hippocampus.","authors":"Marco Bocchio, Artem Vorobyev, Sadra Sadeh, Sophie Brustlein, Robin Dard, Susanne Reichinnek, Valentina Emiliani, Agnes Baude, Claudia Clopath, Rosa Cossart","doi":"10.1371/journal.pbio.3002837","DOIUrl":"10.1371/journal.pbio.3002837","url":null,"abstract":"Inhibitory interneurons are pivotal components of cortical circuits. Beyond providing inhibition, they have been proposed to coordinate the firing of excitatory neurons within cell assemblies. While the roles of specific interneuron subtypes have been extensively studied, their influence on pyramidal cell synchrony in vivo remains elusive. Employing an all-optical approach in mice, we simultaneously recorded hippocampal interneurons and pyramidal cells and probed the network influence of individual interneurons using optogenetics. We demonstrate that CA1 interneurons form a functionally interconnected network that promotes synchrony through disinhibition during awake immobility, while preserving endogenous cell assemblies. Our network model underscores the importance of both cell assemblies and dense, unspecific interneuron connectivity in explaining our experimental findings, suggesting that interneurons may operate not only via division of labor but also through concerted activity.","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11501041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142478510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0