DnaJ mediates phage sensing by the bacterial NLR-related protein bNACHT25.

IF 9.8 1区生物学 Q1 Agricultural and Biological Sciences

PLoS Biology Pub Date : 2025-05-30 eCollection Date: 2025-05-01 DOI:10.1371/journal.pbio.3003203

Amy N Conte, Madison E Ruchel, Samantha M Ridgeway, Emily M Kibby, Toni A Nagy, Aaron T Whiteley

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引用次数: 0

Abstract

Bacteria encode a wide range of antiphage systems and a subset of these proteins are homologous to components of the human innate immune system. Mammalian nucleotide-binding and leucine-rich repeat containing proteins (NLRs) and bacterial NLR-related proteins use a central NACHT domain to link detection of infection with initiation of an antimicrobial response. Bacterial NACHT proteins provide defense against both DNA and RNA phages. Here we investigate the mechanism of phage detection by the bacterial NLR-related protein bNACHT25 in E. coli. bNACHT25 was specifically activated by Emesvirus ssRNA phages and analysis of MS2 phage escaper mutants that evaded detection revealed a critical role for Coat Protein (CP). A genetic assay showed CP was sufficient to activate bNACHT25 but the two proteins did not directly interact. Instead, we found bNACHT25 requires the host chaperone DnaJ to detect CP and protect against phage. Our data support a model in which bNACHT25 detects a wide range of phages using an indirect mechanism that may involve guarding a host cell process rather than binding a specific phage-derived molecule.

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DnaJ通过细菌nlr相关蛋白bNACHT25介导噬菌体感知。

细菌编码广泛的抗噬菌体系统，这些蛋白质的一个子集与人类先天免疫系统的成分同源。哺乳动物核苷酸结合蛋白和富含亮氨酸的重复序列蛋白（NLRs）以及细菌nlr相关蛋白使用一个中央NACHT结构域将感染检测与抗菌反应的启动联系起来。细菌NACHT蛋白提供对DNA和RNA噬菌体的防御。本文研究了细菌nlr相关蛋白bNACHT25在大肠杆菌中检测噬菌体的机制。bNACHT25被Emesvirus ssRNA噬菌体特异性激活，对逃避检测的MS2噬菌体逃逸突变体的分析揭示了外衣蛋白（Coat Protein， CP）的关键作用。基因分析显示CP足以激活bNACHT25，但这两个蛋白并不直接相互作用。相反，我们发现bNACHT25需要宿主伴侣DnaJ来检测CP并保护噬菌体。我们的数据支持一个模型，其中bNACHT25使用一种间接机制检测广泛的噬菌体，该机制可能涉及保护宿主细胞过程，而不是结合特定的噬菌体衍生分子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PLoS Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOLOGY

CiteScore

15.40

自引率

2.00%

发文量

359

审稿时长

3-8 weeks

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