Biodegradation最新文献_第6页

Exploring polyhydroxyalkanoates biosynthesis using hydrocarbons as carbon source: a comprehensive review 探索以碳氢化合物为碳源的聚羟基烷酸酯生物合成：综述

IF 3.1 4区生物学

Biodegradation Pub Date : 2024-02-04 DOI: 10.1007/s10532-023-10068-9

G. Corti Monzón, G. Bertola, M. K. Herrera Seitz, S. E. Murialdo

{"title":"Exploring polyhydroxyalkanoates biosynthesis using hydrocarbons as carbon source: a comprehensive review","authors":"G. Corti Monzón, G. Bertola, M. K. Herrera Seitz, S. E. Murialdo","doi":"10.1007/s10532-023-10068-9","DOIUrl":"10.1007/s10532-023-10068-9","url":null,"abstract":"<div><p>Environmental pollution caused by petrochemical hydrocarbons (HC) and plastic waste is a pressing global challenge. However, there is a promising solution in the form of bacteria that possess the ability to degrade HC, making them valuable tools for remediating contaminated environments and effluents. Moreover, some of these bacteria offer far-reaching potential beyond bioremediation, as they can also be utilized to produce polyhydroxyalkanoates (PHAs), a common type of bioplastics. The accumulation of PHAs in bacterial cells is facilitated in environments with high C/N or C/P ratio, which are often found in HC-contaminated environments and effluents. Consequently, some HC-degrading bacteria can be employed to simultaneously produce PHAs and conduct biodegradation processes. Although bacterial bioplastic production has been thoroughly studied, production costs are still too high compared to petroleum-derived plastics. This article aims to provide a comprehensive review of recent scientific advancements concerning the capacity of HC-degrading bacteria to produce PHAs. It will delve into the microbial strains involved and the types of bioplastics generated, as well as the primary pathways for HC biodegradation and PHAs production. In essence, we propose the potential utilization of HC-degrading bacteria as a versatile tool to tackle two major environmental challenges: HC pollution and the accumulation of plastic waste. Through a comprehensive analysis of strengths and weaknesses in this aspect, this review aims to pave the way for future research in this area, with the goal of facilitating and promoting investigation in a field where obtaining PHAs from HC remains a costly and challenging process.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"519 - 538"},"PeriodicalIF":3.1,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139679768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly approaches for mitigating plastic pollution: advancements and implications for a greener future 减轻塑料污染的生态友好型方法：进展及对更绿色未来的影响

IF 3.1 4区生物学

Biodegradation Pub Date : 2024-02-04 DOI: 10.1007/s10532-023-10062-1

Ayesha Safdar, Fatima Ismail, Maryem Safdar, Muhammad Imran

{"title":"Eco-friendly approaches for mitigating plastic pollution: advancements and implications for a greener future","authors":"Ayesha Safdar, Fatima Ismail, Maryem Safdar, Muhammad Imran","doi":"10.1007/s10532-023-10062-1","DOIUrl":"10.1007/s10532-023-10062-1","url":null,"abstract":"<div><p>Plastic pollution has become a global problem since the extensive use of plastic in industries such as packaging, electronics, manufacturing and construction, healthcare, transportation, and others. This has resulted in an environmental burden that is continually growing, which has inspired many scientists as well as environmentalists to come up with creative solutions to deal with this problem. Numerous studies have been reviewed to determine practical, affordable, and environmentally friendly solutions to regulate plastic waste by leveraging microbes’ innate abilities to naturally decompose polymers. Enzymatic breakdown of plastics has been proposed to serve this goal since the discovery of enzymes from microbial sources that truly interact with plastic in its naturalistic environment and because it is a much faster and more effective method than others. The scope of diverse microbes and associated enzymes in polymer breakdown is highlighted in the current review. The use of co-cultures or microbial consortium-based techniques for the improved breakdown of plastic products and the generation of high-value end products that may be utilized as prototypes of bioenergy sources is highlighted. The review also offers a thorough overview of the developments in the microbiological and enzymatic biological degradation of plastics, as well as several elements that impact this process for the survival of our planet.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"493 - 518"},"PeriodicalIF":3.1,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139679782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of PVCs through in-vitro identification of Bacillus albus and computational pathway analysis of ABH enzyme 通过体外鉴定白僵菌和 ABH 酶的计算途径分析实现聚氯乙烯的生物降解。

IF 3.1 4区生物学

Biodegradation Pub Date : 2024-01-30 DOI: 10.1007/s10532-023-10064-z

Muhammad Naveed, Rida Naveed, Tariq Aziz, Arooj Azeem, Mahrukh Afzal, Muhammad Waseem, Metab Alharbi, Abdulrahman Alshammari, Abdullah F. Alasmari, Thamer H. Albekairi

{"title":"Biodegradation of PVCs through in-vitro identification of Bacillus albus and computational pathway analysis of ABH enzyme","authors":"Muhammad Naveed, Rida Naveed, Tariq Aziz, Arooj Azeem, Mahrukh Afzal, Muhammad Waseem, Metab Alharbi, Abdulrahman Alshammari, Abdullah F. Alasmari, Thamer H. Albekairi","doi":"10.1007/s10532-023-10064-z","DOIUrl":"10.1007/s10532-023-10064-z","url":null,"abstract":"<div><p>Microplastics pose significant challenges to ecosystems and organisms. They can be ingested by marine and terrestrial species, leading to potential health risks and ecological disruptions. This study aims to address the urgent need for effective remediation strategies by focusing on the biodegradation of microplastics, specifically polyvinyl chloride (PVC) derivatives, using the bacterial strain <i>Bacillus albus</i>. The study provides a comprehensive background on the accumulation of noxious substances in the environment and the importance of harnessing biodegradation as an eco-friendly method for pollutant elimination. The specific objective is to investigate the enzymatic capabilities of <i>Bacillus albus</i>, particularly the alpha/beta hydrolases (ABH), in degrading microplastics. To achieve this, in-silico studies were conducted, including analysis of the ABH protein sequence and its interaction with potential inhibitors targeting PVC derivatives. Docking scores of − 7.2 kcal/mol were obtained to evaluate the efficacy of the interactions. The study demonstrates the promising bioremediation prospects of <i>Bacillus albus</i> for microplastics, highlighting its potential as a key player in addressing microplastic pollution. The findings underscore the urgent need for further experimental validation and practical implementation of <i>Bacillus albus</i> in environmental remediation strategies.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 4","pages":"451 - 468"},"PeriodicalIF":3.1,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139574373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of distinctive pyrene-degrading bacteria from an uncontaminated soil 从未遭污染的土壤中分离出独特的芘降解细菌并确定其特征。

IF 3.1 4区生物学

Biodegradation Pub Date : 2024-01-27 DOI: 10.1007/s10532-023-10065-y

Shanshan Sun, Ran Wei, Siyi Hu, Meiyu Yang, Jinzhi Ni

{"title":"Isolation and characterization of distinctive pyrene-degrading bacteria from an uncontaminated soil","authors":"Shanshan Sun, Ran Wei, Siyi Hu, Meiyu Yang, Jinzhi Ni","doi":"10.1007/s10532-023-10065-y","DOIUrl":"10.1007/s10532-023-10065-y","url":null,"abstract":"<div><p>Considerable efforts that isolate and characterize degrading bacteria for polycyclic aromatic hydrocarbons (PAHs) have focused on contaminated environments so far. Here we isolated three distinctive pyrene (PYR)-degrading bacteria from a paddy soil that was not contaminated with PAHs. These included a novel <i>Bacillus</i> sp. PyB-9 and efficient degraders, <i>Shigella</i> sp. PyB-6 and <i>Agromyces</i> sp. PyB-10. All three strains could utilize naphthalene, phenanthrene, anthracene, fluoranthene and PYR as sole carbon sources, and degraded PYR in a range of temperatures (27–37 °C) and pH (5–8). Strains PyB-6 and PyB-10 almost completely degraded 50 mg L<sup>−1</sup> PYR within 15 days, and 75.5% and 98.9% of 100 mg L<sup>−1</sup> PYR in 27 days, respectively. The kinetics of PYR biodegradation was well represented by the Gompertz model. Ten and twelve PYR metabolites were identified in PYR degradation process by strains PyB-6 and PyB-10, respectively. Chemical analyses demonstrated that the degradation mechanisms of PYR were the same for strains PyB-6 and PyB-10 with initial dioxygenation mainly on C-4,5 positions of PYR. The degradation of 4,5-phenanthrenedicarboxylic acid was branched to 4-phenanthrenecarboxylic acid pathway and 5-hydroxy-4-phenanthrenecarboxylic acid pathway, both of which played important roles in PYR degradation by strains PyB-6 and PyB-10. To our knowledge, <i>Shigella</i> sp. and <i>Agromyces</i> sp. were found for the first time to possess the capability for PAHs degradation. These findings contributed to upgrading the bank of microbial resource and knowledge on PAH biodegradation.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"657 - 670"},"PeriodicalIF":3.1,"publicationDate":"2024-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139566321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanically stirred enzymatic membrane reactor containing HRP immobilized on Tau-SiO2@Fe3O4-GO nanocomposite for removal of tetracycline in synthetically concocted wastewater 含有固定在 Tau-SiO2@Fe3O4-GO 纳米复合材料上的 HRP 的机械搅拌酶膜反应器用于去除合成废水中的四环素。

IF 3.1 4区生物学

Biodegradation Pub Date : 2024-01-23 DOI: 10.1007/s10532-023-10066-x

Monireh Besharati Vineh, Amir Ali Poostchi, Davood Kordestani, Mahmoud Karami Qushehbolagh, Ali Akbar Saboury

{"title":"Mechanically stirred enzymatic membrane reactor containing HRP immobilized on Tau-SiO2@Fe3O4-GO nanocomposite for removal of tetracycline in synthetically concocted wastewater","authors":"Monireh Besharati Vineh, Amir Ali Poostchi, Davood Kordestani, Mahmoud Karami Qushehbolagh, Ali Akbar Saboury","doi":"10.1007/s10532-023-10066-x","DOIUrl":"10.1007/s10532-023-10066-x","url":null,"abstract":"<div><p>The process of mechanically stirred membrane reactor containing the suspension of horseradish peroxidase (HRP) immobilized on synthesized nanocomposite (Tau-SiO<sub>2</sub>@Fe<sub>3</sub>O<sub>4</sub>-GO) was designed for continuous degradation of tetracycline. The immobilized HRP was characterized in terms of kinetic parameters and catalytic activities as these parameters were improved highly through immobilization. The stability indices including pH and temperature were investigated in parallel. The immobilized HRP was more tolerable to pH changes as compared to free HRP and the optimum temperature obtained at 40 °C. The reusability of HRP was promoted by immobilization as far as 70% of initial activity after ten cycles. The enzymatic degradation of optimum concentration of tetracycline was carried out in batch condition and 100% of tetracycline removed after 30 min. The results also showed that higher concentration of H<sub>2</sub>O<sub>2</sub> exhibited more oxidation of tetracycline. The optimal ratio of HRP/H<sub>2</sub>O<sub>2</sub> was also obtained at 0.005. The simultaneous process including separation and the biocatalytic degradation established in the membrane stirrer reactor concluded that no amount of tetracycline was observed in the permeate stream coming from the membrane after 30 min of operation.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 4","pages":"407 - 422"},"PeriodicalIF":3.1,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139519475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigation of Desulfovibrio ferrophilus IS5 degradation of X80 carbon steel mechanical properties using a green biocide 使用绿色杀菌剂缓解铁锈脱硫弧菌 IS5 对 X80 碳钢机械性能的降解。

IF 3.1 4区生物学

Biodegradation Pub Date : 2024-01-23 DOI: 10.1007/s10532-023-10063-0

Zhong Li, Jike Yang, Shihang Lu, Wenwen Dou, Tingyue Gu

{"title":"Mitigation of Desulfovibrio ferrophilus IS5 degradation of X80 carbon steel mechanical properties using a green biocide","authors":"Zhong Li, Jike Yang, Shihang Lu, Wenwen Dou, Tingyue Gu","doi":"10.1007/s10532-023-10063-0","DOIUrl":"10.1007/s10532-023-10063-0","url":null,"abstract":"<div><p>Most microbiologically influenced corrosion (MIC) studies focus on the threat of pinhole leaks caused by MIC pitting. However, microbes can also lead to structural failures. Tetrakis hydroxymethyl phosphonium sulfate (THPS) biocide mitigated the microbial degradation of mechanical properties of X80 steel pipeline by <i>Desulfovibrio ferrophilus</i> (IS5 strain), a very corrosive sulfate reducing bacterium. It was found that 100 ppm (w/w) THPS added to the enriched artificial seawater (EASW) culture medium before incubation resulted in 2.8-log reduction in sessile cell count after a 7-d incubation at 28 °C under anaerobic conditions, leading to 94% uniform corrosion rate reduction (from 1.3 to 0.07 mm/a), and 84% pitting corrosion rate reduction (from 0.70 to 0.11 mm/a). The X80 dogbone coupon incubated with 100 ppm THPS for 7 d suffered 3% loss in ultimate tensile strain and 0% loss in ultimate tensile strength compared with the abiotic control in EASW. In comparison, the no-treatment X80 dogbone coupon suffered losses of 13% in ultimate tensile strain and 6% in ultimate tensile stress, demonstrating very good THPS efficacy.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 4","pages":"439 - 449"},"PeriodicalIF":3.1,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139519479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of bacterial community in a rapid composting method using 16SrDNA genes sequencing 利用16SrDNA基因测序快速堆肥法鉴定细菌群落。

IF 3.1 4区生物学

Biodegradation Pub Date : 2023-11-21 DOI: 10.1007/s10532-023-10056-z

Muhammad Shahid, Waqar Ahmed, Abdul Basit Khan, Zafar Iqbal Shams, Afsheen Arif

{"title":"Identification of bacterial community in a rapid composting method using 16SrDNA genes sequencing","authors":"Muhammad Shahid, Waqar Ahmed, Abdul Basit Khan, Zafar Iqbal Shams, Afsheen Arif","doi":"10.1007/s10532-023-10056-z","DOIUrl":"10.1007/s10532-023-10056-z","url":null,"abstract":"<div><p>Composting is a process of microbial degradation of organic waste and is commonly applied for waste management. This is a slow process and requires a lot of land and human resources. The present study investigated mechanical augmentation with required microbial culture for composting municipal solid waste (MSW). Thirty isolates were subjected to 16S rDNA PCR amplification and gene sequencing. The isolates' sequencing from the compost samples was processed on BLASTn. Fourteen strains were identified for further experiments. The results divulge that Empedobacter (04), Bacillus (02), Proteus (02), Lactiplantibacillus (01), Klebsiella (01), Citrobacter (01), Brevibacillus (01), <i>E. coli</i> (01) and one unidentified strain were growing during composting. Eleven combinations of bacterial consortium and respective additives were applied for the organic waste decomposition in the next stage, resulting in varied completion periods ranging from 3 to 14 days. Two combinations were completed within 3 days, which are considered ideal combinations for composting. The microbial consortium was significantly diverse, which is a reason for rapid biodegradation. The present study reveals that the technology will be highly feasible for municipal solid waste management in tropical/subtropical countries.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 4","pages":"361 - 370"},"PeriodicalIF":3.1,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138175174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-based polyester-polyurethane foams: synthesis and degradability by Aspergillus niger and Aspergillus clavatus 生物基聚酯-聚氨酯泡沫:黑曲霉和克拉曲霉的合成及其降解性。

IF 3.1 4区生物学

Biodegradation Pub Date : 2023-11-21 DOI: 10.1007/s10532-023-10059-w

Mara L. Polo, Karen Russell-White, Santiago E. Vaillard, Luis Ríos, Gregorio R. Meira, Diana A. Estenoz, Marisa E. Spontón

{"title":"Bio-based polyester-polyurethane foams: synthesis and degradability by Aspergillus niger and Aspergillus clavatus","authors":"Mara L. Polo, Karen Russell-White, Santiago E. Vaillard, Luis Ríos, Gregorio R. Meira, Diana A. Estenoz, Marisa E. Spontón","doi":"10.1007/s10532-023-10059-w","DOIUrl":"10.1007/s10532-023-10059-w","url":null,"abstract":"<div><p>In this article, the degradability by <i>Aspergillus niger and Aspergillus clavatus</i> of three bio-based polyurethane (PU) foams is compared to previous degradability studies involving a <i>Pseudomonas</i> sp. bacterium and similar initial materials (Spontón et al. in Int. Biodet. Biodeg. 85:85–94, 2013, https://doi.org/10.1016/j.ibiod.2013.05.019). First, three new polyester-polyurethane foams were prepared from mixtures of castor oil (CO), maleated castor oil (MACO), toluene diisocyanate (TDI), and water. Then, their degradation tests were carried out in an aqueous medium, and employing the two mentioned fungi, after their isolation from the environment. From the degradation tests, the following was observed: (a) the insoluble (and slightly collapsed) foams exhibited free hydroxyl, carboxyl, and amine moieties; and (b) the water soluble (and low molar mass) compounds contained amines, carboxylic acids, and glycerol. The most degraded foam contained the highest amount of MACO, and therefore the highest concentration of hydrolytic bonds. A basic biodegradation mechanism was proposed that involves hydrolysis and oxidation reactions.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138175173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organic stabilization and methane production under different organic loading rates in UASB treating swine wastewater 不同有机负荷量下UASB处理猪场废水的有机稳定性和甲烷产量。

IF 3.1 4区生物学

Biodegradation Pub Date : 2023-11-15 DOI: 10.1007/s10532-023-10060-3

Jacineumo Falcão de Oliveira, Ronaldo Fia, Ana Flavia Santos Rabelo de Melo, Fátima Resende Luiz Fia, Fernando Neris Rodrigues, Luciene Alves Batista Siniscalchi, Mateus Pimentel de Matos

{"title":"Organic stabilization and methane production under different organic loading rates in UASB treating swine wastewater","authors":"Jacineumo Falcão de Oliveira, Ronaldo Fia, Ana Flavia Santos Rabelo de Melo, Fátima Resende Luiz Fia, Fernando Neris Rodrigues, Luciene Alves Batista Siniscalchi, Mateus Pimentel de Matos","doi":"10.1007/s10532-023-10060-3","DOIUrl":"10.1007/s10532-023-10060-3","url":null,"abstract":"<div><p>This study proposes the was to evaluate the stability and methane production with organic load differents in an upflow anaerobic sludge blanket reactor (UASB) treating swine wastewater by methods of multivariate analysis. Four organic loads were used with average hydraulic holding times of one day. The methods of data analysis of linear regression, Pearson correlation, principal component analysis and hierarchical clustering analysis were used for understanding stability and methane production in the reactor. The highest concentrations of bicarbonate alkalinity of 683 mg L<sup>−1</sup> CaCO<sub>3</sub> and total volatile acids of 1418 mg L<sup>−1</sup> HAc with maximum organic loading applied were obtained. The optimal stability conditions occurred at an intermediate and partial alkalinity ratio between 0.24 and 0.25 observed in initial phases with a chemical oxygen demand (COD) removal of 47–57%. Maximum methane production was 9.0 L CH<sub>4</sub> d<sup>−1</sup> observed with linear regression positive and occurred at the highest applied organic load, corresponding to the highest COD removal efficiency and increased microbial biomass. Positive and negative correlation between functional stability in anaerobic digestion showed regular activity between acids, alkalinity and organic matter removal. This fact was also proven by the analysis of principal components that showed three components responsible for explaining 83.2% of the data variability, and the alkalinity, organic matter influent and organic acids had the greatest effects on the stability of the UASB reactor. Hierarchical clusters detected the formation of five groupings with a similarity of 50.1%, indicating that temperature and pH were variables with unitary influences on data dimensionality.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 4","pages":"389 - 405"},"PeriodicalIF":3.1,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107589912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of low-density polythene (LDPE) by a novel strain of Pseudomonas aeruginosa WD4 isolated from plastic dumpsite 从塑料垃圾场分离的新型铜绿假单胞菌WD4对低密度聚乙烯（LDPE）的生物降解。

IF 3.1 4区生物学

Biodegradation Pub Date : 2023-11-06 DOI: 10.1007/s10532-023-10061-2

Shilpa, Nitai Basak, Sumer Singh Meena

{"title":"Biodegradation of low-density polythene (LDPE) by a novel strain of Pseudomonas aeruginosa WD4 isolated from plastic dumpsite","authors":"Shilpa, Nitai Basak, Sumer Singh Meena","doi":"10.1007/s10532-023-10061-2","DOIUrl":"10.1007/s10532-023-10061-2","url":null,"abstract":"<div><p>The present study was proposed with the idea to screen and isolate efficient low-density polyethylene (LDPE) degrading novel bacterial strains from the plastic-contaminated dumping site. The identification of the bacterial isolate was performed with the help of microbiological and molecular characterization approaches. The screening of the best isolate was performed based on its growth in Bushnell-Hass broth supplemented with LDPE sheets as the sole carbon source. The molecular characterization revealed that the isolate WD4 showed a similarity with the <i>Pseudomonas aeruginosa</i> species. A comparative analysis of <i>Pseudomonas aeruginosa</i> WD4 identified in the current study with <i>Pseudomonas putida</i> MTCC 2445 strain was performed. The present study demonstrated that the bacterial isolate showed 9.2% degradation of LDPE films while <i>Pseudomonas putida</i> revealed a 6.5% weight reduction after 100 days of incubation at 37 °C. The end products of the LDPE degradation were analysed using Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC–MS). The LDPE degradation products eluted include fatty acids such as octadecanoic, hexadecanoic acid, dodecanal, and octyl palmitoleate, alkanes, and some of the unknown compounds after 100 days of microbial treatment with the isolated strain. The detailed analysis of the by-products generated in the current study indicates their contribution to the biochemical pathway of LDPE degradation. The profound scope lies in the scalability of these bacterial strains at the industrial level to combat the LDPE waste and similar plastic garbage problems, globally.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"641 - 655"},"PeriodicalIF":3.1,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71476605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0