Biodegradation assessment tests of biopolymers in standardised water: different sources of variability

Abstract

This study assessed the ultimate biodegradation degree of two resins, polyhydroxybutyrate and polylactic acid (PHB and PLA), and three commercial biobased bags (BMAT, BGREEN, and BBEIGE) through the measurement of oxygen consumption in closed respirometers. Activated sludge from a wastewater treatment plant (WWTP) was used as the inoculum, cellulose was used as the reference material, and five trials were conducted with two different devices under identical conditions, with a 28-day incubation period. The results revealed statistically significant differences in the biochemical oxygen demand (BOD) measurements for cellulose, PHB, and PLA between the two devices and within the same devices across different trials. The degree of biodegradation (D_t), calculated as the percentage of theoretical oxygen demand (ThOD), varied depending on the device and trial. For cellulose, D_t ranged from 61 to 93%; for PLA, the maximum Dt was 6%; and for PHB, D_t oscillated between 16 and 72%. These findings highlight the critical importance of carefully selecting the testing equipment, as it significantly influences biodegradation results, in addition to the already known interlaboratory variability caused by the inoculum.