Biodegradation最新文献_第5页

Correction: Physiological and transcriptomic response of enriched anammox culture upon elevated hydrazine exposure 更正：高浓度厌氧氨氧化培养在高联氨暴露下的生理和转录组反应。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-07-08 DOI: 10.1007/s10532-025-10151-3

Tugba Sari, Kozet Yapsakli, Deniz Akgul, Bulent Mertoglu

引用次数: 0

Chitin degradation by the aquatic pigment-producing bacteria of Janthinobacterium spp. from stream flows and springs of Shulgan River basin and Shulgan-Tash Cave, Southern Urals 乌拉尔南部舒尔干河流域及舒尔干—塔什溶洞水流、泉水中水生色素产菌Janthinobacterium sp.降解几丁质

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-07-06 DOI: 10.1007/s10532-025-10155-z

Gleb E. Aktuganov, Elena A. Gilvanova, Polina Yu. Milman, Ravil R. Garafutdinov, Alena S. Ryabova, Nailya F. Galimzianova, Olga Ya. Chervyatsova, Lyudmila Yu. Kuzmina

{"title":"Chitin degradation by the aquatic pigment-producing bacteria of Janthinobacterium spp. from stream flows and springs of Shulgan River basin and Shulgan-Tash Cave, Southern Urals","authors":"Gleb E. Aktuganov, Elena A. Gilvanova, Polina Yu. Milman, Ravil R. Garafutdinov, Alena S. Ryabova, Nailya F. Galimzianova, Olga Ya. Chervyatsova, Lyudmila Yu. Kuzmina","doi":"10.1007/s10532-025-10155-z","DOIUrl":"10.1007/s10532-025-10155-z","url":null,"abstract":"<div><p>Six chitinolytic strains of <i>Janthinobacterium</i> were isolated from the springs and reservoirs in Shulgan-Tash cave, which is not only one of largest caves in Southern Urals with preserved Paleolithic painting dating back to about 20,000 years, but also a final link in the karst hydrosystem of Shulgan River basin. This study aimed to characterize chitin degradation by the isolated bacteria for comprehension of their involvement in carbon cycle proceeding in the local groundwater ecosystem. The isolates varied in their colony morphology and pigmentation; five of the strains produced violacein-like pigments, while the sole isolate synthesized red pigment similar to prodigiosin. All the isolates were identified as <i>Janthinobacterium</i> sp. based on 16S rRNA gene sequence, where five strains were clustered with most homology to type species, <i>J. lividum</i>, and the single strain, IB-RH, was located separately from this group on phylogenetic trees. The studied bacteria manifested psychrotolerant properties with temperature optima of growth and chitin destruction at 22–26 °C. The isolates generally produced extracellular chitinase in range 0.14–0.18 U/mL; the maximal enzyme’s yield reached to 6–8 days. The violacein-producing strain IB-ST-GO exhibited most rapid dynamics of the chitinase secretion together with highest growth indices and degradation degree of various chitinous substrates. The noticeable chitinase production by this strain and other isolates along with their ability to colonize and assimilate diverse chitinous substrates of crustaceans’ origin as sole carbon source evidence their potential contribution in processes of chitin degradation in the karstic and non-karstic groundwaters.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144566904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioconversion of food waste to poly (3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer using Stenotrophomonas geniculata: study of operating parameters and process optimization 利用窄养单胞菌将食物垃圾生物转化为聚（3-羟基丁酸酯-co-3-羟基戊酸酯）生物聚合物：操作参数及工艺优化研究。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-07-05 DOI: 10.1007/s10532-025-10156-y

Hanieh Karimnezhad, Farshad Rahimpour, Sara Samadi

{"title":"Bioconversion of food waste to poly (3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer using Stenotrophomonas geniculata: study of operating parameters and process optimization","authors":"Hanieh Karimnezhad, Farshad Rahimpour, Sara Samadi","doi":"10.1007/s10532-025-10156-y","DOIUrl":"10.1007/s10532-025-10156-y","url":null,"abstract":"<div><p>In order to address the growing concerns regarding the environmental impact of plastics and the related environmental implications of food waste, this study was conducted with the objective of producing poly(3-hydroxybutyrate-<i>co</i>-3-hydroxyvalerate) (PHBV) by <i>Stenotrophomonas geniculata</i> bacteria isolated from a municipal landfill and utilizing food waste as a substrate. FTIR, <sup>1</sup>H-NMR, GC–MS and thermal analyses were used to confirm the production of PHBV. The produced biopolymer has thermoplastic elastomer properties similar to those of natural rubber. Temperature, concentration of nitrogen and carbon sources and pH were considered as the main factors for optimizing PHBV production. At the optimum conditions, i.e., temperature 32.3 °C, pH 9, nitrogen and glucose concentrations of 13.25 g/L and 27.71 g/L, respectively, maximum produced PHBV and yield of 2.835 g/L, and 0.746 g/g cell dry weight were obtained empirically. Notably, this study is the first to demonstrate the use of the <i>S. geniculata</i> strain Flmat 1 species for the production of PHBV using structurally unrelated simple carbon source. This study's strategy deals with the global burden of food waste and subsequently produces the biopolymer, which is a waste-to-wealth conversion.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144566903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiota and methanogenic activities in an anaerobic internal circulation reactor: insights into biogas production from brewery wastewater 厌氧内循环反应器中的微生物群和产甲烷活性：啤酒废水生产沼气的见解。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-06-28 DOI: 10.1007/s10532-025-10153-1

Cecilia Callejas, Leandro Guerrero, Leonardo Erijman, Iván López, Liliana Borzacconi

{"title":"Microbiota and methanogenic activities in an anaerobic internal circulation reactor: insights into biogas production from brewery wastewater","authors":"Cecilia Callejas, Leandro Guerrero, Leonardo Erijman, Iván López, Liliana Borzacconi","doi":"10.1007/s10532-025-10153-1","DOIUrl":"10.1007/s10532-025-10153-1","url":null,"abstract":"<div><p>In this study, we analyzed the prokaryotic community and methanogenic activities in sludge samples collected from a full-scale internal circulation (IC) reactor used to treat brewery wastewater. The reactor performance was monitored over 15 months, and specific methanogenic activities were periodically measured in fresh sludge samples using CO<sub>2</sub>/H<sub>2</sub> or acetate as substrates. The maximum hydrogenotrophic activities were consistently higher than maximum acetoclastic activities, suggesting the relevance of hydrogenotrophic methanogens in the sludge. Over six months, the prokaryotic community present in four sludge samples was analyzed using amplicon libraries and metagenomics. V4-16S rRNA amplicon libraries revealed the presence of a diverse microbial community dominated by Firmicutes and Bacteroidetes among bacterial phyla, and Halobacterota and Euryarchaeota among archaea. Furthermore, the 16S libraries constructed with cDNA were consistent with the methanogenic activity assays. A genome-centric metagenomics approach was used to assemble 42 high-quality metagenome-assembled genomes (MAGs), among which <i>Methanothrix</i> and <i>Methanobacterium</i> were the dominant archaeal members, and Acidobacteriota, Synergistota, Krumholzibacteriota, and Nitrospirota phyla were among the bacteria. Potential acetogenic members were explored via the <i>fths</i> gene; 15 MAGs contained this marker gene. A combination of methanogenic activity tests, amplicon libraries, and MAG analysis was used to gain insights into the prokaryotic structure and functional potential of the microbial community driving methane production in the reactor.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-based optimization of biogas and methane yields in UASB reactors for treating domestic wastewater 基于机器学习的UASB反应器处理生活污水沼气和甲烷产量优化。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-06-26 DOI: 10.1007/s10532-025-10152-2

Saurabh Kumar, Saurabh Kumar, Divesh Ranjan Kumar, Dayanand Sharma, Warit Wipulanusat

{"title":"Machine learning-based optimization of biogas and methane yields in UASB reactors for treating domestic wastewater","authors":"Saurabh Kumar, Saurabh Kumar, Divesh Ranjan Kumar, Dayanand Sharma, Warit Wipulanusat","doi":"10.1007/s10532-025-10152-2","DOIUrl":"10.1007/s10532-025-10152-2","url":null,"abstract":"<div><p>This study aimed to optimize biogas and methane production from Up-flow anaerobic sludge blanket reactors for treating domestic wastewater using advanced machine learning models—namely, eXtreme Gradient Boosting (XGBoost) and its hybridized form, XGBoost, integrated with particle swarm optimization (XGBoost-PSO). The key operational variables included time, flow rate, chemical oxygen demand (COD), pH, volatile fatty acids, total suspended solids, hydraulic retention time, alkalinity, and the organic loading rate. Empirical data used to train and validate the predictive models were acquired from the sequential treatment of laboratory-prepared low-strength synthetic wastewater and actual municipal wastewater samples. Data was collected from two treatment phases: synthetic wastewater (COD: 335.45 ± 28.32 mg/L) was treated from days 0 to 270, followed by real domestic wastewater (COD: 225.28 ± 65.98 mg/L) from days 0 to 130. Gas production was continuously monitored throughout. The XGBoost-PSO model outperformed the standard XGBoost algorithm in both the training and testing phases. For biogas prediction during training, XGBoost-PSO achieved an RMSE of 0.0405, an MAE of 0.0225, and an R<sup>2</sup> of 0.9832, whereas for methane, the values were an RMSE of 0.0257, an MAE of 0.0175, and an R<sup>2</sup> of 0.9942. The testing results further confirmed the model’s robustness, with RMSE, MAE, and R<sup>2</sup> values of 0.1017, 0.0676, and 0.9404 for biogas and 0.0694, 0.0519, and 0.9717 for methane, respectively. These findings highlight the potential of integrating artificial intelligence-driven approaches to optimize bioenergy recovery in wastewater treatment systems.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of the C9 aromatic hydrocarbon n-propylbenzene by Sphingobium barthaii KK22 and coexistence of multiple biodegradation pathways barthaii Sphingobium KK22对C9芳烃正丙苯的利用及多种生物降解途径的共存

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-06-23 DOI: 10.1007/s10532-025-10149-x

Miharu Sakai, Jiro F. Mori, Robert A. Kanaly

{"title":"Utilization of the C9 aromatic hydrocarbon n-propylbenzene by Sphingobium barthaii KK22 and coexistence of multiple biodegradation pathways","authors":"Miharu Sakai, Jiro F. Mori, Robert A. Kanaly","doi":"10.1007/s10532-025-10149-x","DOIUrl":"10.1007/s10532-025-10149-x","url":null,"abstract":"<div><p><i>n</i>-Propylbenzene is an environmental pollutant belonging to a class of heavily used nonpolar alkylated aromatic solvents referred to as the C9 aromatics. Although <i>n</i>-propylbenzene is detected in different environmental matrices and displays toxicity, its bacterial biodegradation has been little explored. Consequently, few transformation products have been identified, and comprehensive biodegradation pathways were not constructed. Understanding <i>n</i>-propylbenzene biotransformation shall be useful to predict its fate and transport in the environment. Therefore, <i>n</i>-propylbenzene biotransformation by soil bacterium, <i>Sphingobium barthaii</i> KK22, was examined by liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI–MS/MS) through product ion scan collision induced dissociation (CID) analyses. Targeted CID of unknown biotransformation products resulted in the proposal of structures for at least 18 compounds and based upon these results, metabolites were organized into biotransformation pathways which revealed multiple routes to the TCA cycle. Decarboxylation of the <i>n</i>-propylbenzene alkyl side chain was proposed as a key part of the biodegradation process—so-called alkyl chain shortening. At the same time, the aromatic ring of <i>n</i>-propylbenzene was vulnerable to dioxygenation no matter the alkyl chain length or degree of alkyl chain oxidation resulting in numerous 3-, 2- and 1-carbon chain length compounds and their aromatic ring-opened counterparts. Quantitative analyses by LC and growth monitoring by absorbance confirmed that this bacterium eliminated 100 mg/L <i>n</i>-propylbenzene from culture media and that it utilized <i>n</i>-propylbenzene as a carbon source. In the natural environment, catabolically versatile soil sphingomonads such as <i>S. barthaii</i> may be contributors to the biodegradation of alkylated aromatic nonpolar pollutants such as <i>n</i>-propylbenzene.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of pretilachlor and butachlor by novel bacterial strains isolated from paddy field soil 稻田土壤中分离的新型细菌降解甲草胺和丁草胺的研究。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-06-21 DOI: 10.1007/s10532-025-10148-y

Nguyen Thi Oanh, Ha Danh Duc

{"title":"Biodegradation of pretilachlor and butachlor by novel bacterial strains isolated from paddy field soil","authors":"Nguyen Thi Oanh, Ha Danh Duc","doi":"10.1007/s10532-025-10148-y","DOIUrl":"10.1007/s10532-025-10148-y","url":null,"abstract":"<div><p>In this study, two pretilachlor-degrading bacterial strains isolated from soil, <i>Enterobacter</i> sp<i>.</i> Pre1 and <i>Pseudomonas</i> sp. Pre2 completely utilized the compound as a sole carbon, energy and nitrogen source under aerobic conditions. The determination of degradation kinetics revealed that the rates of both isolates followed the Michaelis–Menten model, in which the maximum utilization rates of <i>Enterobacter</i> sp. Pre1 and <i>Pseudomonas</i> sp. Pre2 were 0.010 ± 0.0012 and 0.0060 ± 0.0007 mM/h, respectively. Moreover, <i>Pseudomonas</i> sp. Pre2 exhibited effective degradation of butachlor. <i>Enterobacter</i> sp. Pre1 showed better biofilm formation than the later one. Their immobilized biomass in polyurethane foam (PUF) reached 323.4 ± 35.6 mg/g PUF completely degrading pretilachlor at 0.15 mM within 12 h in a packed bed bioreactor. A metabolite, 2,6-diethylaniline, was produced during the degradation by both strains. Besides, 4-amino-3,5-diethyl phenol and aniline were the metabolites in the degradation by <i>Enterobacter</i> sp. Pre1 and <i>Pseudomonas</i> sp. Pre2, respectively. This study confirmed the efficiency and mechanisms in the degradation of pretilachlor by freely suspended and immobilized cells of the isolated bacteria.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation of Cr(VI) using novel thermophilic bacteria Brevibacillus borstelensis SSAU-3 T: optimization, mechanism and phytotoxicity study 新型嗜热细菌短芽孢杆菌ssau - 3t对铬（VI）的生物修复：优化、机制及植物毒性研究

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-06-20 DOI: 10.1007/s10532-025-10145-1

Manshi Agrawal, Abhijeet Sharma, Akanksha Singh, Shanthy Sundaram

{"title":"Bioremediation of Cr(VI) using novel thermophilic bacteria Brevibacillus borstelensis SSAU-3 T: optimization, mechanism and phytotoxicity study","authors":"Manshi Agrawal, Abhijeet Sharma, Akanksha Singh, Shanthy Sundaram","doi":"10.1007/s10532-025-10145-1","DOIUrl":"10.1007/s10532-025-10145-1","url":null,"abstract":"<div><p>Rapid industrialization and advancement of chemical fertilizers in agriculture get infused with water causing heavy pollution of inorganic pollutants has become a serious problem. This research focuses on the utilization of a thermophilic bacteria <i>Brevibacillus borstelensis,</i> SSAU-3 T in the bioremediation of hexavalent chromium (Cr (VI)). The strain has capability in > 99% removal of 40 ppm Cr (VI). Further optimization was studied by varying parameters (pH, Inoculum size, salinity, volume and temperature) based on Cr(VI) removal capabilities. Removal mechanism was determined by studying thermodynamic, kinetic, and isotherm under optimized parameters: pH 7, salinity 5 g/L, inoculum size 2%, medium volume 20 mL, temperature 55 °C which resulted that Redlich-Peterson isotherm model is a best fit for this study. Characterization of functional groups and bonds present on bacterial cell surface before and after treatment with chromium were optimized by Fourier Transform Infrared Spectroscopy and surface morphology changes were also observed by Scanning Electron Microscopy. A phytotoxicity study was conducted on wheat, which showed that bacterial secondary metabolites were not toxic. The study highlights an eco-friendly and cost-effective approach to mitigate Cr(VI) toxicity using thermophilic microbes from hot springs.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycotransformation of anthracene by indigenous Trichoderma lixii and Talaromyces pinophilus isolates: insights into the metabolic pathways, enzyme profiles and acute toxicity 原生立希木霉和嗜松木霉菌株对蒽的菌转化：代谢途径、酶谱和急性毒性的见解。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-06-18 DOI: 10.1007/s10532-025-10147-z

Samson O. Egbewale, Ajit Kumar, Mduduzi P. Mokoena, Ademola O. Olaniran

{"title":"Mycotransformation of anthracene by indigenous Trichoderma lixii and Talaromyces pinophilus isolates: insights into the metabolic pathways, enzyme profiles and acute toxicity","authors":"Samson O. Egbewale, Ajit Kumar, Mduduzi P. Mokoena, Ademola O. Olaniran","doi":"10.1007/s10532-025-10147-z","DOIUrl":"10.1007/s10532-025-10147-z","url":null,"abstract":"<div><p>This study focused on the mycotransformation of a very prominent PAH, anthracene, and its acute toxicity reduction by Ascomycete fungi: <i>Trichoderma lixii</i> strain FLU1 (<i>Tl</i>FLU1) and <i>Talaromyces pinophilus</i> strain FLU12 (<i>Tp</i>FLU12), indigenously isolated from benzo[b] fluoranthene-enriched activated sludge. The results indicate that both the isolates <i>Tl</i>FLU1 and <i>Tp</i>FLU12 could tolerate anthracene exposure up to 1000 mg/L, with increased expression of ligninolytic enzymes: Laccase, Lignin peroxidase, and Manganese peroxidase. The mycotransformation of anthracene was observed to be growth-linked and mediated by the expression of the intracellular enzymes as the initial mechanism used by these strains followed by the ligninolytic enzymes with up to 56% and 38% anthracene degradation by <i>Tl</i>FLU1 and <i>Tp</i>FLU12, respectively, after 24 days with a concomitant change in pH from 5 to 4 (<i>Tl</i>FLU1) and 6.2 (<i>Tp</i>FLU12). The GC–MS and FTIR analysis of the samples indicate the appearance of metabolic intermediates: 9,10 anthracenedione and benzoic acid in <i>Tl</i>FLU1 grown medium, while anthrone and 9,10 anthracenedione were detected in <i>Tp</i>FLU12 grown medium. The mycotransformation of the compound followed a first-order kinetic model with an effective concentration (EC<sub>50</sub>) of 262.3–266.1 mg/L, with a toxicity unit (TU) of 0.4% in <i>Vibrio parahaemolyticus</i> (6 h exposure) to each intermediate. Results show efficient mycotransformation of anthracene into a non-toxic state by <i>Tl</i>FLU1 and <i>Tp</i>FLU12.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of pH on the biodegradation efficiency of fats, oils, and grease by biosurfactant-producing bacterial consortia pH值对产生生物表面活性剂的细菌群落对脂肪、油和油脂生物降解效率的影响。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-06-16 DOI: 10.1007/s10532-025-10146-0

Jia-Ying Wong, Ngui-Sing Ngieng, Ahmad Husaini, Rosmawati Saat, Hasnain Hussain

{"title":"Influence of pH on the biodegradation efficiency of fats, oils, and grease by biosurfactant-producing bacterial consortia","authors":"Jia-Ying Wong, Ngui-Sing Ngieng, Ahmad Husaini, Rosmawati Saat, Hasnain Hussain","doi":"10.1007/s10532-025-10146-0","DOIUrl":"10.1007/s10532-025-10146-0","url":null,"abstract":"<div><p>The accumulation of fats, oils, and grease (FOG) in wastewater systems presents major environmental challenges, necessitating the development of effective bioremediation strategies. Biosurfactant-producing bacteria are promising for FOG degradation; however, their efficacy is highly pH-dependent, affecting microbial metabolism and biosurfactant stability. This study evaluates the impact of pH on FOG biodegradation by locally isolated biosurfactant-producing bacterial consortia to identify optimal pH conditions. Two highly efficient biosurfactant-producing bacterial isolates, identified via 16S rRNA sequencing as <i>Pseudomonas aeruginosa</i> and <i>Bacillus velezensis</i>, were cultured in Bushnell Haas (BH) medium to form a bacterial consortium. The consortium was then inoculated into fresh BH medium, adjusted to pH values from 4 to 9, and supplemented with 1% FOG (w/v). Samples were monitored at six-day intervals for 30 days under continuous shaking at 130 rpm. After 30 days of biodegradation, the solid FOGs in pH 6 disappeared while flocs were observed in both pH 4 and 5. Despite greater floc formation at pH 6, GC–MS analysis revealed that pH 4 achieved the highest degradation rate, displaying the fewest FOG peaks and the lowest area under peaks, indicating the most substantial FOG reduction. Notably, the consortium achieved the highest FOG removal at pH 4, an acidic condition under which most long-chain FOG components were completely degraded or transformed into shorter chains. This finding reveals an unexpected optimum pH 4 for FOG bioremediation by two efficient biosurfactant-producing bacteria combined into a synergistic consortium, highlighting a potential strategy to enhance grease waste treatment.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0