Clinical and Experimental Reproductive Medicine-CERM最新文献_第5页

Serum miR-329-3p as a potential biomarker for poor ovarian response in an in vitro fertilization. 血清miR-329-3p作为体外受精卵巢不良反应的潜在生物标志物。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2025-01-21 DOI: 10.5653/cerm.2024.07094

Jung Hoon Kim, Hye-Ok Kim, Su-Yeon Lee, Eun-A Park, Kyoung Hee Choi, Kiye Kang, Eun Jeong Yu, Mi Kyoung Koong, Kyung-Ah Lee

{"title":"Serum miR-329-3p as a potential biomarker for poor ovarian response in an in vitro fertilization.","authors":"Jung Hoon Kim, Hye-Ok Kim, Su-Yeon Lee, Eun-A Park, Kyoung Hee Choi, Kiye Kang, Eun Jeong Yu, Mi Kyoung Koong, Kyung-Ah Lee","doi":"10.5653/cerm.2024.07094","DOIUrl":"10.5653/cerm.2024.07094","url":null,"abstract":"Objective: Several miRNAs have been identified as differentially expressed in patients with poor ovarian response (POR) compared to those with normal responses. This study aims to assess the potential of serum miR-329-3p as a biomarker for diagnosing POR.Methods: We conducted a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis to confirm the target genes of miR-329-3p. KGN cells were transfected with both miR-329-3p mimic and inhibitor to assess the differential expression of these target genes. In accordance with the Bologna criteria, we enrolled 16 control patients and 16 patients with POR. We collected patient samples, including serum from day 2 and the human chorionic gonadotropin (hCG) day, as well as granulosa and cumulus cells, to validate the expression of miR-329-3p using quantitative real-time polymerase chain reaction.Results: KEGG pathway analysis revealed that miR-329-3p targeted adenylyl cyclase 9 (ADCY9) and protein kinase A subunit beta (PRKACB), both of which are involved in ovarian steroidogenesis. In KGN cells treated with a miR-329-3p mimic, ADCY9 and PRKACB expression levels were significantly reduced (p<0.05). Elevated levels of miR-329-3p suppressed aromatase expression and 17β-estradiol production by modulating ADCY9 and PRKACB in KGN cells. These effects were also observed in POR patients. Follicle-stimulating hormone receptor (FSHR) expression was diminished in the granulosa cells of POR patients. On day 2, on hCG day, and in granulosa cells, miR-329-3p exhibited high expression levels in the serum of POR patients.Conclusion: miR-329-3p exhibited increased expression in granulosa cells and in the sera of POR patients. Consequently, we propose that miR-329-3p may be a potential biomarker for the diagnosis of POR.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"52 1","pages":"44-55"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced strategies for single embryo selection in assisted human reproduction: A review of clinical practice and research methods. 人类辅助生殖中单胚胎选择的先进策略：临床实践和研究方法综述。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-06-10 DOI: 10.5653/cerm.2023.06478

Zahra Bashiri, Azita Afzali, Morteza Koruji, Hossein Torkashvand, Mehrdad Ghorbanlou, Nadia Sheibak, Zahra Zandieh, Fatemehsadat Amjadi

{"title":"Advanced strategies for single embryo selection in assisted human reproduction: A review of clinical practice and research methods.","authors":"Zahra Bashiri, Azita Afzali, Morteza Koruji, Hossein Torkashvand, Mehrdad Ghorbanlou, Nadia Sheibak, Zahra Zandieh, Fatemehsadat Amjadi","doi":"10.5653/cerm.2023.06478","DOIUrl":"10.5653/cerm.2023.06478","url":null,"abstract":"Among the primary objectives of contemporary assisted reproductive technology research are achieving the births of healthy singletons and improving overall fertility outcomes. Substantial advances have been made in refining the selection of single embryos for transfer, with the aim of maximizing the likelihood of successful implantation. The principal criterion for this selection is embryo morphology. Morphological evaluation systems are based on traditional parameters, including cell count and fragmentation, pronuclear morphology, cleavage rate, blastocyst formation, and various sequential embryonic assessments. To reduce the incidence of multiple pregnancies and to identify the single embryo with the highest potential for growth, invasive techniques such as preimplantation genetic screening are employed in in vitro fertilization clinics. However, new approaches have been suggested for clinical application that do not harm the embryo and that provide consistent, accurate results. Noninvasive technologies, such as time-lapse imaging and omics, leverage morphokinetic parameters and the byproducts of embryo metabolism, respectively, to identify noninvasive prognostic markers for competent single embryo selection. While these technologies have garnered considerable interest in the research community, they are not incorporated into routine clinical practice and still have substantial room for improvement. Currently, the most promising strategies involve integrating multiple methodologies, which together are anticipated to increase the likelihood of successful pregnancy.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"8-29"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Severe ovarian hyperstimulation syndrome in an oocyte donor despite preventive strategies. 尽管采取了预防策略，一名卵母细胞捐献者仍患上了严重的卵巢过度刺激综合征。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-07-22 DOI: 10.5653/cerm.2024.06884

Antonio Forgiarini, Mariana Selene Paredes Contreras, Silvia Bontá, Sara Maggi, Luis Alberto Quintero Espinel

引用次数: 0

Low-air-pressure clean room system: A flexible, high-quality model for assisted reproduction laboratories. 低压无尘室系统：一个灵活的，高质量的模型辅助生殖实验室。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-06-13 DOI: 10.5653/cerm.2024.06793

Minh Tam Le, Trung Van Nguyen, Thai Thanh Thi Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen

{"title":"Low-air-pressure clean room system: A flexible, high-quality model for assisted reproduction laboratories.","authors":"Minh Tam Le, Trung Van Nguyen, Thai Thanh Thi Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen","doi":"10.5653/cerm.2024.06793","DOIUrl":"10.5653/cerm.2024.06793","url":null,"abstract":"Objective: This study aimed to develop a configurable clean room paradigm with low air pressure for assisted reproductive technology (ART) clinics and demonstrate the concept's efficacy using in vitro fertilization (IVF) treatment.Methods: A high-standard clean room system with positive pressure (13 Pa) was built using accessible materials and equipment for ART laboratories. Methods for controlling and evaluating the clean room's characteristics were developed and implemented for quality assessment and calibration to maximize efficiency. The feasibility of the flexible clean room concept was assessed by analyzing the key performance indicators of embryo culture and IVF treatment.Results: After 3 weeks of testing, the concentration of particles ≥0.5 μm was 6.04 times lower than the International Organization for Standardization (ISO) class 5 standard (3,520 particles/m3) in the IVF laboratory. Air pressure, noise, temperature, and humidity were controlled stably and appropriately. Five days after installation and handover, the volatile organic compound concentration dropped to 0.00 ppm. With blastocysts and a respectable blastocyst rate, embryonic culture with female patients younger than 40 matched the criteria (63.5% and 38.9%, respectively). After vitrified blastocysts were transferred, the pregnancy and implantation rates were 58.5% and 36.2%, respectively, demonstrating a high degree of treatment success.Conclusion: Our customizable, high-quality, low-air-pressure clean room model can be implemented to achieve positive outcomes for infertility treatment.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"52 1","pages":"56-64"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preservation of ovarian function using human pluripotent stem cell-derived mesenchymal progenitor cells. 利用源自人类多能干细胞的间充质祖细胞保护卵巢功能。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-05-24 DOI: 10.5653/cerm.2024.07003

Dong Ryul Lee, Jeoung Eun Lee

{"title":"Preservation of ovarian function using human pluripotent stem cell-derived mesenchymal progenitor cells.","authors":"Dong Ryul Lee, Jeoung Eun Lee","doi":"10.5653/cerm.2024.07003","DOIUrl":"10.5653/cerm.2024.07003","url":null,"abstract":"Ovarian reserve diminishes with age, and older women experience a corresponding shift in sex hormone levels. These changes contribute to an age-dependent decrease in fertility and a decline in overall health. Furthermore, while survival rates following cancer treatment have improved for young female patients, a reduction in ovarian function due to the side effects of such treatments can be difficult to avoid. To date, no effective therapy has been recommended to preserve ovarian health in these patients. Mesenchymal progenitor cells (MPCs) are considered a promising option for cell therapy aimed at maintaining fertility and fecundity. Although MPCs derived from human adult tissues are recognized for their various protective effects against ovarian senescence, they are limited in quantity. Consequently, human pluripotent stem cell-derived MPCs (hPSC-MPCs), which exhibit high proliferative capacity and retain genetic stability during growth, have been utilized to delay reproductive aging. This review highlights the impact of hPSC-MPCs on preserving the functionality of damaged ovaries in female mouse models subjected to chemotherapy and natural aging. It also proposes their potential as a valuable cell source for fertility preservation in women with a variety of diseases.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"30-37"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved ovarian adiponectin system expression in polycystic ovary syndrome treated with exenatide. 用艾塞那肽治疗多囊卵巢综合征可改善卵巢脂肪生成素系统的表达。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-08-01 DOI: 10.5653/cerm.2024.06912

Asma Vatankhah, Mohabbat Jamhiri, Sima Vatankhah, Keivan Lorian, Mohammad Ebrahim Rezvani, Mahin Izadi

{"title":"Improved ovarian adiponectin system expression in polycystic ovary syndrome treated with exenatide.","authors":"Asma Vatankhah, Mohabbat Jamhiri, Sima Vatankhah, Keivan Lorian, Mohammad Ebrahim Rezvani, Mahin Izadi","doi":"10.5653/cerm.2024.06912","DOIUrl":"10.5653/cerm.2024.06912","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that can cause infertility. This experimental study was conducted to elucidate the role of adiponectin signaling in rats with PCOS treated with exenatide. Twenty-eight adult female Wistar rats were divided into four groups of seven. The normal group did not receive any drug. The PCOS+vehicle (Veh) group received estradiol valerate to induce PCOS, then was divided into PCOS +E50 and PCOS+E100 groups and treated with 50 or 100 mg/kg doses of exenatide, respectively. The mRNA expression of adiponectin and adiponectin receptor 1 (Adipo-R1) was evaluated using a semi-quantitative real-time polymerase chain reaction. The results indicated that the level of adiponectin diminished in the PCOS rats while exenatide increased adiponectin expression at both doses. Adiponectin receptor mRNA levels were higher in the PCOS rats than in the normal rats (p<0.05). In addition, exenatide decreased the levels of Adipo-R1 expression. Taken together, our results showed that exenatide may improve PCOS characteristics in rats through the molecular regulation of adiponectin and its receptor.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"98-100"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal exposure to phenanthrene induces testicular apoptosis and Sertoli cell dysfunction in F1 adult male mice: a histological and molecular study. 母体暴露于菲会诱发 F1 成年雄性小鼠睾丸凋亡和 Sertoli 细胞功能障碍：一项组织学和分子研究。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-07-22 DOI: 10.5653/cerm.2024.07038

Azar Afshar, Hamid Nazarian, Fatemeh Fadaefathabadi, Fakhroddin Aghajanpour, Reza Soltani, Mohammad-Amin Abdollahifar, Gholamreza Hassanzadeh, Mohsen Nourozian

{"title":"Maternal exposure to phenanthrene induces testicular apoptosis and Sertoli cell dysfunction in F1 adult male mice: a histological and molecular study.","authors":"Azar Afshar, Hamid Nazarian, Fatemeh Fadaefathabadi, Fakhroddin Aghajanpour, Reza Soltani, Mohammad-Amin Abdollahifar, Gholamreza Hassanzadeh, Mohsen Nourozian","doi":"10.5653/cerm.2024.07038","DOIUrl":"10.5653/cerm.2024.07038","url":null,"abstract":"Objective: Phenanthrene, a polycyclic aromatic hydrocarbon, is found in abundance in environmental pollutants, food, and drinking water. This substance can accumulate in body tissues and exert harmful effects. Moreover, phenanthrene can cross the placental barrier, potentially impacting fetal development. We aimed to explore the impacts of maternal exposure to phenanthrene on testicular tissue and Sertoli cell function in F1 mice.Methods: Female rats with vaginal plugs were randomly assigned to one of three groups: control, sham, or phenanthrene. The control group received no intervention during pregnancy. In the sham and phenanthrene groups, corn oil and a phenanthrene solution, respectively, were administered via gavage once every 2 days. Offspring were separated by sex 21 days after birth. At 56 days postnatal, male F1 offspring were euthanized, and their testes were harvested for histological and molecular analyses.Results: Phenanthrene exposure was associated with a lower testicular weight and volume, a smaller diameter of the seminiferous tubules, and a relative thinning of the germinal epithelium. These changes were associated with increased cellular apoptosis, as shown by the upregulation of caspase 3 expression. Additionally, we observed an increase in vacuolization and residual bodies within the tissue. Conversely, the number of Sertoli cells and expression levels of Sox9, as well as the Ocln and Itgb1 genes, were found to be lowered.Conclusion: Maternal exposure to phenanthrene impacts both germ cells and Sertoli cells, disrupting their function and leading to fertility disorders in male F1 offspring mice.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"87-97"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro fertilization as an option for couples with genetic disorders. 将体外受精作为患有遗传性疾病的夫妇的一种选择。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-06-10 DOI: 10.5653/cerm.2023.06667

F Yudha Christianti, Legiran Legiran

{"title":"In vitro fertilization as an option for couples with genetic disorders.","authors":"F Yudha Christianti, Legiran Legiran","doi":"10.5653/cerm.2023.06667","DOIUrl":"10.5653/cerm.2023.06667","url":null,"abstract":"Errors in human DNA may cause genetic disorders. Technological developments have raised hopes for reducing the risks of genetic inheritance among married couples who have a history of such disorders. Among the developments in reproductive health technology that reduce those risks is the in vitro fertilization (IVF) process. This review aimed to describe the current strategies using IVF and preimplantation genetic testing (PGT), which would be effective for couples with genetic disorders to have healthy offspring. The literature review included full-text, open-access research articles from ScienceDirect, PubMed, and Google Scholar that were published between 2013 and 2023, with 65 articles obtained from various journals. The keywords were 'in vitro fertilization,' 'reproductive genetic disorders,' 'PGT-A,' 'PGT-M,' 'PGT-SR,' and 'oocyte donor.' A total of 46 articles were selected as the most relevant to the review topic, and the results show that the IVF process can be an option for couples with a history of genetic disorders. Several additional procedures can be performed following IVF, such as oocyte donation and PGT, to help couples who want to have offspring without transmitting their genetic disorders. IVF can be an option for couples who have or carry genetic disorders. With IVF, couples can undertake several procedures such as oocyte donation and PGT for aneuploidy, monogenic disorders, or structural rearrangement.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"1-7"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution of chromosomal aberrations to the pathogenesis of primary and secondary amenorrhea: A study from Western Iran. 染色体畸变对原发性和继发性闭经发病机制的影响：伊朗西部的一项研究。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-07-22 DOI: 10.5653/cerm.2024.06807

Azam Azimi, Matin Heidari, Reza Alibakhshi, Sara Hamani, Negar Salimi, Parham Nejati, Nazanin Jalilian

{"title":"Contribution of chromosomal aberrations to the pathogenesis of primary and secondary amenorrhea: A study from Western Iran.","authors":"Azam Azimi, Matin Heidari, Reza Alibakhshi, Sara Hamani, Negar Salimi, Parham Nejati, Nazanin Jalilian","doi":"10.5653/cerm.2024.06807","DOIUrl":"10.5653/cerm.2024.06807","url":null,"abstract":"Objective: Amenorrhea is an abnormal condition characterized by the absence of menstruation in women of reproductive age. According to the World Health Organization, amenorrhea ranks as the sixth leading cause of female infertility. Approximately 2% to 5% of women of reproductive age experience amenorrhea, which can be classified as primary amenorrhea (PA) or secondary amenorrhea (SA). Several studies have named chromosomal abnormalities among the main causes of amenorrhea, though the prevalence of these abnormalities may differ across populations. The objective of this study was to ascertain the frequency and types of chromosomal abnormalities in women with amenorrhea in Kermanshah Province, Iran.Methods: This retrospective study included patients with PA and SA who underwent standard cytogenetic analysis. We also conducted a review of the literature on chromosomal abnormalities and their prevalence in SA.Results: Among the 137 cases of PA in this study, 22% exhibited chromosomal abnormalities. Numerical changes were the most common finding (46.6%) in this group, including 45,X, mosaic, and 47,XXX karyotypes. These were followed by the 46,XY karyotype (40%). Of the 51 cases of SA that received chromosomal analysis, abnormalities were identified in only one case. Additionally, our review of the literature revealed that chromosomal aberrations are responsible for 7% of SA cases globally.Conclusion: In this study, we successfully characterized the cytogenetic causes of PA and SA in a substantial population from Kermanshah Province, Iran.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"65-70"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitrification alters growth differentiation factor 9 and follicle-stimulating hormone receptor expression in human cumulus-mural granulosa cells. 玻璃化技术改变了人类积膜颗粒细胞中生长分化因子 9 和卵泡刺激素受体的表达。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-12-01 Epub Date: 2024-05-17 DOI: 10.5653/cerm.2023.06198

Batara Sirait, Budi Wiweko, Nining Handayani, Ayu Mulia Sundari, Raden Muharam, Ahmad Aulia Jusuf, Dwi Anita Suryandari, Ichramsjah A Rachman, Indah Suci Widyahening, Arief Boediono

{"title":"Vitrification alters growth differentiation factor 9 and follicle-stimulating hormone receptor expression in human cumulus-mural granulosa cells.","authors":"Batara Sirait, Budi Wiweko, Nining Handayani, Ayu Mulia Sundari, Raden Muharam, Ahmad Aulia Jusuf, Dwi Anita Suryandari, Ichramsjah A Rachman, Indah Suci Widyahening, Arief Boediono","doi":"10.5653/cerm.2023.06198","DOIUrl":"10.5653/cerm.2023.06198","url":null,"abstract":"Objective: Ovarian tissue vitrification is widely utilized for fertility preservation in prepubertal and adolescent female patients with cancer. The current literature includes reports of successful pregnancy and live birth following autografting. However, the effects of the vitrification process on cumulus-mural granulosa cells (C-mGCs)-somatic cells in ovarian tissue crucial for oocyte maturation and early embryonic development-remain unclear. This study was conducted to explore the impact of vitrification on the cellular function of C-mGCs by quantifying the expression of growth differentiation factor 9 (GDF-9), bone morphogenetic protein 15 (BMP-15), follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), connexin 37, survivin, and caspase 3.Methods: Mature and immature C-mGCs were obtained from 38 women with polycystic ovary syndrome who participated in an in vitro fertilization program. The C-mGCs were then divided into two groups: fresh and vitrified. The expression levels of target genes were assessed using real-time quantitative polymerase chain reaction.Results: After vitrification, GDF-9 expression was significantly decreased among both mature and immature C-mGCs, with 0.2- and 0.1-fold changes, respectively (p<0.01). Similarly, FSHR expression in the mature and immature groups was reduced by 0.1- and 0.02-fold, respectively, following vitrification (p<0.01). The expression levels of the other genes, including BMP-15, LHR, connexin 37, survivin, and caspase 3, remained similar across the examined groups (p>0.05).Conclusion: Vitrification may compromise oocyte maturation through reduced GDF-9 and FSHR expression in C-mGCs after warming.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"293-300"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0