Clinical and Experimental Reproductive Medicine-CERM最新文献_第10页

Ameliorative effects of propolis upon reproductive toxicity in males. 蜂胶对雄性生殖毒性的改善作用。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2023-03-01 DOI: 10.5653/cerm.2022.05785

Saleem Ali Banihani

引用次数: 0

Sleep deprivation induces structural changes in the adult rat testis: The protective effects of olive oil. 睡眠剥夺引起成年大鼠睾丸结构变化:橄榄油的保护作用。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2023-03-01 DOI: 10.5653/cerm.2022.05624

Fatemeh Karimi, Ali Noorafshan, Saied Karbalay-Doust, Maryam Naseh

{"title":"Sleep deprivation induces structural changes in the adult rat testis: The protective effects of olive oil.","authors":"Fatemeh Karimi, Ali Noorafshan, Saied Karbalay-Doust, Maryam Naseh","doi":"10.5653/cerm.2022.05624","DOIUrl":"https://doi.org/10.5653/cerm.2022.05624","url":null,"abstract":"Objective: Sleep deprivation (SD) is a common problem in today's stressful lifestyle and have physiological consequences, including reproductive dysfunction and infertility. As an antioxidant, olive oil may be effective in reducing testicular and spermatological damage by decreasing the production of free radicals.Methods: This study investigated the effects of olive oil on sperm quality and testicular structure using stereological methods to assess rats with SD.Results: When comparing SD group to grid floor+distilled water (GR) group, we found that the sperm count and motility, as well as the percentage of slow progressive sperm was significantly lower in SD group (p<0.05), but the percentage of immotile sperm was higher (p<0.01). However, no improvement was observed in sperm count or motility after concomitant treatment of SD group with olive oil. Stereological examinations revealed no significant change in the total volumes of the seminiferous tubules, interstitial tissue, and germinal epithelium in the study groups. Conversely, the total number of testicular cell types was significantly lower in SD group than in GR group. Although the total number of Sertoli and Leydig cells was significantly higher in the SD+olive oil group than in the untreated SD group, no significant difference in the total number of other testicular cell types was observed between the two groups.Conclusion: SD potentially induced structural changes in testis that affected sperm count and motility. However, olive oil only improved the total number of Sertoli and Leydig cells in the animals with SD and did not improve sperm count and motility.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"50 1","pages":"19-25"},"PeriodicalIF":1.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/43/c6/cerm-2022-05624.PMC10030206.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9214582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of human chorionic gonadotropin-producing peripheral blood mononuclear cells on the endometrial receptivity and implantation sites of the mouse uterus. 人绒毛膜促性腺激素生成外周血单个核细胞对小鼠子宫内膜容受性及着床部位的影响。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05358

Delsuz Rezaee, Mojgan Bandehpour, Bahram Kazemi, Sara Hosseini, Zeinab Dehghan, Saiyad Bastaminejad, Mohammad Salehi

{"title":"Effects of human chorionic gonadotropin-producing peripheral blood mononuclear cells on the endometrial receptivity and implantation sites of the mouse uterus.","authors":"Delsuz Rezaee, Mojgan Bandehpour, Bahram Kazemi, Sara Hosseini, Zeinab Dehghan, Saiyad Bastaminejad, Mohammad Salehi","doi":"10.5653/cerm.2022.05358","DOIUrl":"https://doi.org/10.5653/cerm.2022.05358","url":null,"abstract":"Objective: This research investigated the effects of human chorionic gonadotropin (HCG)-producing peripheral blood mononuclear cells (PBMCs) on the implantation rate and embryo attachment in mice.Methods: In this experimental study, a DNA fragment of the HCG gene was cloned into an expression vector, which was transfected into PBMCs. The concentration of the produced HCG was measured using enzyme-linked immunosorbent assay. Embryo attachment was investigated on the co-cultured endometrial cells and PBMCs in vitro. As an in vivo experiment, intrauterine administration of PBMCs was done in plaque-positive female mice. Studied mice were distributed into five groups: control, embryo implantation dysfunction (EID), EID with produced HCG, EID with PBMCs, and EID with HCG-producing PBMCs. Uterine horns were excised to characterize the number of implantation sites and pregnancy rate on day 7.5 post-coitum. During an implantation window, the mRNA expression of genes was evaluated using real-time polymerase chain reaction.Results: DNA fragments were cloned between the BamHI and EcoRI sites in the vector. About 465 pg/mL of HCG was produced in the transfected PBMCs. The attachment rate, pregnancy rate, and the number of implantation sites were substantially higher in the HCG-producing PBMCs group than in the other groups. Significantly elevated expression of the target genes was observed in the EID with HCG-producing PBMCs group.Conclusion: Alterations in gene expression following the intrauterine injection of HCG-producing PBMCs, could be considered a possible cause of increased embryo attachment rate, pregnancy rate, and the number of implantation sites.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 4","pages":"248-258"},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/98/dc/cerm-2022-05358.PMC9732076.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10361113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Commentary on the new 2022 European Society of Human Reproduction and Embryology (ESHRE) endometriosis guidelines. 对新的2022年欧洲人类生殖与胚胎学会(ESHRE)子宫内膜异位症指南的评论。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05603

Eun Hee Yu, Jong Kil Joo

引用次数: 2

Therapeutic effect of Ferula assa-foetida oleo-gum resin in rats with letrozole-induced polycystic ovary syndrome. 阿魏油胶树脂对来曲唑诱导的多囊卵巢综合征大鼠的治疗作用。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05449

Amir Shieh, Seyyed Majid Bagheri, Maryam Yadegari, Davoud Javidmehr, Zeinab Farhadi

{"title":"Therapeutic effect of Ferula assa-foetida oleo-gum resin in rats with letrozole-induced polycystic ovary syndrome.","authors":"Amir Shieh, Seyyed Majid Bagheri, Maryam Yadegari, Davoud Javidmehr, Zeinab Farhadi","doi":"10.5653/cerm.2022.05449","DOIUrl":"https://doi.org/10.5653/cerm.2022.05449","url":null,"abstract":"Objective: Asafoetida is a gum derived from Ferula assa-foetida, which is used in traditional Iranian medicine to treat some reproductive system disorders. The effects of asafoetida on ovarian tissue, expression of certain genes associated with polycystic ovary syndrome (PCOS), and levels of liver, kidney, and blood cell factors after treatment in a rat model were investigated.Methods: Thirty rats were divided into five groups: normal, polycystic, and treatment with three doses of asafoetida (12.5, 25, and 50 mg/kg for 3 weeks after PCOS induction). PCOS was induced by letrozole at a dose of 1 mg/kg administered orally for 3 weeks. Blood samples were taken, and the ovaries were removed and prepared for histomorphometric examination. Liver and kidney parameters were measured. The mRNA expression levels of luteinizing hormone receptor, CYP11A1, adenosine monophosphate-activated protein kinase, adiponectin, and adiponectin receptors 1 and 2 were also measured by real-time polymerase chain reaction.Results: The levels of liver, kidney, and blood parameters did not significantly differ between the treatment groups and the control group. At doses of 25 and 50 mg/kg, ovarian histopathology, especially the thicknesses of the theca and granulosa layers, was significantly improved relative to the PCOS group. The expression of target genes also improved in the 25 and 50 mg/kg treatment groups.Conclusion: Asafoetida can be used to treat PCOS as a complementary approach to conventional therapies. Asafoetida appears to act by regulating and activating metabolic and ovarian cycle enzymes.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 4","pages":"239-247"},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4c/cd/cerm-2022-05449.PMC9732079.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10361114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A rare case of primary ovarian mesenchymal chondrosarcoma in pregnancy. 妊娠期原发性卵巢间充质软骨肉瘤1例。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05372

Sepideh Azizi, Elena Ghotbi, Setare Nassiri

引用次数: 0

Evaluation of polyglycolic acid as an animal-free biomaterial for three-dimensional culture of human endometrial cells. 聚乙醇酸作为无动物生物材料用于人子宫内膜细胞三维培养的评价。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05302

Sadegh Amiri, Zohreh Bagher, Azadeh Akbari Sene, Reza Aflatoonian, Mehdi Mehdizadeh, Peiman Broki Milan, Leila Ghazizadeh, Mahnaz Ashrafi, FatemehSadat Amjadi

{"title":"Evaluation of polyglycolic acid as an animal-free biomaterial for three-dimensional culture of human endometrial cells.","authors":"Sadegh Amiri, Zohreh Bagher, Azadeh Akbari Sene, Reza Aflatoonian, Mehdi Mehdizadeh, Peiman Broki Milan, Leila Ghazizadeh, Mahnaz Ashrafi, FatemehSadat Amjadi","doi":"10.5653/cerm.2022.05302","DOIUrl":"https://doi.org/10.5653/cerm.2022.05302","url":null,"abstract":"Objective: Animal-free scaffolds have emerged as a potential foundation for consistent, chemically defined, and low-cost materials. Because of its good potential for high biocompatibility with reproductive tissues and well-characterized scaffold design, we investigated whether polyglycolic acid (PGA) could be used as an animal-free scaffold instead of natural fibrin-agarose, which has been used successfully for three-dimensional human endometrial cell culture.Methods: Isolated primary endometrial cells was cultured on fibrin-agarose and PGA polymers and evaluated various design parameters, such as scaffold porosity and mean fiber diameter. Cytotoxicity, scanning electron microscopy (SEM), and immunostaining experiments were conducted to examine cell activity on fabricated scaffolds.Results: The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay and SEM results showed that endometrial cells grew and proliferated on both scaffolds. Immunostaining showed cytokeratin and vimentin expression in seeded cells after 7 days of culture. On both scaffolds, an epithelial arrangement of cultured cells was found on the top layer and stromal arrangement matrix on the bottom layer of the scaffolds. Therefore, fibrin-agarose and PGA scaffolds successfully mimicked the human endometrium in a way suitable for in vitro analysis.Conclusion: Both fibrin-agarose and PGA scaffolds could be used to simulate endometrial structures. However, because of environmental and ethical concerns and the low cost of synthetic polymers, we recommend using PGA as a synthetic polymer for scaffolding in research instead of natural biomaterials.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 4","pages":"259-269"},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f6/39/cerm-2022-05302.PMC9732078.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10361112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Coenzyme Q10 improves sperm motility and antioxidant status in infertile men with idiopathic oligoasthenospermia. 辅酶Q10改善特发性少弱精子症不育男性的精子活力和抗氧化状态。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05463

Ahmed T Alahmar

{"title":"Coenzyme Q10 improves sperm motility and antioxidant status in infertile men with idiopathic oligoasthenospermia.","authors":"Ahmed T Alahmar","doi":"10.5653/cerm.2022.05463","DOIUrl":"https://doi.org/10.5653/cerm.2022.05463","url":null,"abstract":"Objective: Oxidative stress is a key player in the development of idiopathic male infertility (IMI), and various antioxidants have been used for the treatment of IMI with inconsistent results. Coenzyme Q10 (CoQ10) is a cofactor and an antioxidant that may improve semen parameters and reduce oxidative stress in patients with idiopathic oligoasthenospermia (OA). Therefore, this study aimed to explore the effect of CoQ10 on semen parameters and antioxidant markers in patients with idiopathic OA.Methods: Fifty patients with idiopathic OA and 35 fertile controls were enrolled in this prospective controlled study. All participants underwent a comprehensive fertility assessment. All patients received CoQ10 (300 mg/day) orally once daily for 3 months. Semen parameters, seminal CoQ10 levels, reactive oxygen species (ROS) levels, total antioxidant capacity (TAC), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were measured in patients and controls at the start of the study and after 3 months.Results: Treatment with CoQ10 resulted in increased sperm progressive motility (p<0.05), total motility (p<0.01), seminal TAC (p<0.01), SOD (p<0.05), GPx (p<0.001), and seminal CoQ10 (p<0.001) levels and reduced ROS (p<0.01) in patients as compared to baseline. Sperm concentration and motility were also significantly correlated with antioxidant measures and seminal CoQ10 levels (r=0.38-0.57).Conclusion: CoQ10 therapy (300 mg/day for 3 months) improved sperm motility and seminal antioxidant markers in patients with idiopathic OA. Therefore, CoQ10 could be a promising treatment for patients with idiopathic infertility and may improve their fertility potential.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 4","pages":"277-284"},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e9/6b/cerm-2022-05463.PMC9732077.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10729947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Prolonged semen incubation alters the biological characteristics of human spermatozoa. 长时间的精液孵育改变了人类精子的生物学特性。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05435

Sayed Abbas Datli Beigi, Mohammad Ali Khalili, Ali Nabi, Mohammad Hosseini, Abolghasem Abbasi Sarcheshmeh, Mojdeh Sabour

{"title":"Prolonged semen incubation alters the biological characteristics of human spermatozoa.","authors":"Sayed Abbas Datli Beigi, Mohammad Ali Khalili, Ali Nabi, Mohammad Hosseini, Abolghasem Abbasi Sarcheshmeh, Mojdeh Sabour","doi":"10.5653/cerm.2022.05435","DOIUrl":"https://doi.org/10.5653/cerm.2022.05435","url":null,"abstract":"Objective: The present study assessed the biological characteristics of human spermatozoa at different time intervals (0, 1, 1.5, and 2 hours) after incubation at 37°C.Methods: Twenty-five normozoospermic semen samples were incubated at 37°C. Incubation was performed at four time intervals of 0 (after liquefaction), 1, 1.5, and 2 hours. The samples were evaluated for sperm parameters at each time interval.Results: The rate of sperm progressive motility decreased at 1.5 hours compared to 0 hours as well as 2 hours compared to 1 hour and 0 hours. The rate of non-motile spermatozoa also decreased after 2 hours compared to after 0 hours. No significant changes were observed in sperm viability (p=0.98) and non- progressive motility (p=0.48) at any time intervals. Abnormal sperm morphology increased at 1.5 hours of incubation time (p<0.001). No significant changes were observed in DNA fragmentation at 1 hour compared to 0 hours (median [interquartile range]: 19.5 [4] vs. 19 [4]), as well as at 1.5 hours compared to 1 hour (20 [5]). However, a significant increase in DNA fragmentation was observed at 1.5 hours compared to 0 hours. The mitochondrial membrane potential decreased remarkably after 1 hour of incubation time. No significant differences were observed in the acrosome reaction or malonaldehyde levels at any time point (p=0.34 and p=0.98, respectively).Conclusion: The incubation of normozoospermic samples before use in assisted reproductive technology should be less than 1.5 hours to minimize the destructive effects of prolonged incubation time on general and specific sperm parameters.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 4","pages":"270-276"},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f8/e5/cerm-2022-05435.PMC9732074.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10361117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive evaluation of embryo quality for the selection of transferable embryos in human in vitro fertilization-embryo transfer. 人类体外受精-胚胎移植中可移植胚胎选择的无创胚胎质量评价。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05575

Jihyun Kim, Jaewang Lee, Jin Hyun Jun

{"title":"Non-invasive evaluation of embryo quality for the selection of transferable embryos in human in vitro fertilization-embryo transfer.","authors":"Jihyun Kim, Jaewang Lee, Jin Hyun Jun","doi":"10.5653/cerm.2022.05575","DOIUrl":"https://doi.org/10.5653/cerm.2022.05575","url":null,"abstract":"The ultimate goal of human assisted reproductive technology is to achieve a healthy pregnancy and birth, ideally from the selection and transfer of a single competent embryo. Recently, techniques for efficiently evaluating the state and quality of preimplantation embryos using time-lapse imaging systems have been applied. Artificial intelligence programs based on deep learning technology and big data analysis of time-lapse monitoring system during in vitro culture of preimplantation embryos have also been rapidly developed. In addition, several molecular markers of the secretome have been successfully analyzed in spent embryo culture media, which could easily be obtained during in vitro embryo culture. It is also possible to analyze small amounts of cell-free nucleic acids, mitochondrial nucleic acids, miRNA, and long non-coding RNA derived from embryos using real-time polymerase chain reaction (PCR) or digital PCR, as well as next-generation sequencing. Various efforts are being made to use non-invasive evaluation of embryo quality (NiEEQ) to select the embryo with the best developmental competence. However, each NiEEQ method has some limitations that should be evaluated case by case. Therefore, an integrated analysis strategy fusing several NiEEQ methods should be urgently developed and confirmed by proper clinical trials.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 4","pages":"225-238"},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a8/50/cerm-2022-05575.PMC9732075.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10729946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2