Clinical and Experimental Reproductive Medicine-CERM最新文献_第10页

A rare case of primary ovarian mesenchymal chondrosarcoma in pregnancy. 妊娠期原发性卵巢间充质软骨肉瘤1例。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05372

Sepideh Azizi, Elena Ghotbi, Setare Nassiri

引用次数: 0

Evaluation of polyglycolic acid as an animal-free biomaterial for three-dimensional culture of human endometrial cells. 聚乙醇酸作为无动物生物材料用于人子宫内膜细胞三维培养的评价。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05302

Sadegh Amiri, Zohreh Bagher, Azadeh Akbari Sene, Reza Aflatoonian, Mehdi Mehdizadeh, Peiman Broki Milan, Leila Ghazizadeh, Mahnaz Ashrafi, FatemehSadat Amjadi

{"title":"Evaluation of polyglycolic acid as an animal-free biomaterial for three-dimensional culture of human endometrial cells.","authors":"Sadegh Amiri, Zohreh Bagher, Azadeh Akbari Sene, Reza Aflatoonian, Mehdi Mehdizadeh, Peiman Broki Milan, Leila Ghazizadeh, Mahnaz Ashrafi, FatemehSadat Amjadi","doi":"10.5653/cerm.2022.05302","DOIUrl":"https://doi.org/10.5653/cerm.2022.05302","url":null,"abstract":"Objective: Animal-free scaffolds have emerged as a potential foundation for consistent, chemically defined, and low-cost materials. Because of its good potential for high biocompatibility with reproductive tissues and well-characterized scaffold design, we investigated whether polyglycolic acid (PGA) could be used as an animal-free scaffold instead of natural fibrin-agarose, which has been used successfully for three-dimensional human endometrial cell culture.Methods: Isolated primary endometrial cells was cultured on fibrin-agarose and PGA polymers and evaluated various design parameters, such as scaffold porosity and mean fiber diameter. Cytotoxicity, scanning electron microscopy (SEM), and immunostaining experiments were conducted to examine cell activity on fabricated scaffolds.Results: The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay and SEM results showed that endometrial cells grew and proliferated on both scaffolds. Immunostaining showed cytokeratin and vimentin expression in seeded cells after 7 days of culture. On both scaffolds, an epithelial arrangement of cultured cells was found on the top layer and stromal arrangement matrix on the bottom layer of the scaffolds. Therefore, fibrin-agarose and PGA scaffolds successfully mimicked the human endometrium in a way suitable for in vitro analysis.Conclusion: Both fibrin-agarose and PGA scaffolds could be used to simulate endometrial structures. However, because of environmental and ethical concerns and the low cost of synthetic polymers, we recommend using PGA as a synthetic polymer for scaffolding in research instead of natural biomaterials.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 4","pages":"259-269"},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f6/39/cerm-2022-05302.PMC9732078.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10361112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Coenzyme Q10 improves sperm motility and antioxidant status in infertile men with idiopathic oligoasthenospermia. 辅酶Q10改善特发性少弱精子症不育男性的精子活力和抗氧化状态。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05463

Ahmed T Alahmar

{"title":"Coenzyme Q10 improves sperm motility and antioxidant status in infertile men with idiopathic oligoasthenospermia.","authors":"Ahmed T Alahmar","doi":"10.5653/cerm.2022.05463","DOIUrl":"https://doi.org/10.5653/cerm.2022.05463","url":null,"abstract":"Objective: Oxidative stress is a key player in the development of idiopathic male infertility (IMI), and various antioxidants have been used for the treatment of IMI with inconsistent results. Coenzyme Q10 (CoQ10) is a cofactor and an antioxidant that may improve semen parameters and reduce oxidative stress in patients with idiopathic oligoasthenospermia (OA). Therefore, this study aimed to explore the effect of CoQ10 on semen parameters and antioxidant markers in patients with idiopathic OA.Methods: Fifty patients with idiopathic OA and 35 fertile controls were enrolled in this prospective controlled study. All participants underwent a comprehensive fertility assessment. All patients received CoQ10 (300 mg/day) orally once daily for 3 months. Semen parameters, seminal CoQ10 levels, reactive oxygen species (ROS) levels, total antioxidant capacity (TAC), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were measured in patients and controls at the start of the study and after 3 months.Results: Treatment with CoQ10 resulted in increased sperm progressive motility (p<0.05), total motility (p<0.01), seminal TAC (p<0.01), SOD (p<0.05), GPx (p<0.001), and seminal CoQ10 (p<0.001) levels and reduced ROS (p<0.01) in patients as compared to baseline. Sperm concentration and motility were also significantly correlated with antioxidant measures and seminal CoQ10 levels (r=0.38-0.57).Conclusion: CoQ10 therapy (300 mg/day for 3 months) improved sperm motility and seminal antioxidant markers in patients with idiopathic OA. Therefore, CoQ10 could be a promising treatment for patients with idiopathic infertility and may improve their fertility potential.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 4","pages":"277-284"},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e9/6b/cerm-2022-05463.PMC9732077.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10729947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Prolonged semen incubation alters the biological characteristics of human spermatozoa. 长时间的精液孵育改变了人类精子的生物学特性。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05435

Sayed Abbas Datli Beigi, Mohammad Ali Khalili, Ali Nabi, Mohammad Hosseini, Abolghasem Abbasi Sarcheshmeh, Mojdeh Sabour

{"title":"Prolonged semen incubation alters the biological characteristics of human spermatozoa.","authors":"Sayed Abbas Datli Beigi, Mohammad Ali Khalili, Ali Nabi, Mohammad Hosseini, Abolghasem Abbasi Sarcheshmeh, Mojdeh Sabour","doi":"10.5653/cerm.2022.05435","DOIUrl":"https://doi.org/10.5653/cerm.2022.05435","url":null,"abstract":"Objective: The present study assessed the biological characteristics of human spermatozoa at different time intervals (0, 1, 1.5, and 2 hours) after incubation at 37°C.Methods: Twenty-five normozoospermic semen samples were incubated at 37°C. Incubation was performed at four time intervals of 0 (after liquefaction), 1, 1.5, and 2 hours. The samples were evaluated for sperm parameters at each time interval.Results: The rate of sperm progressive motility decreased at 1.5 hours compared to 0 hours as well as 2 hours compared to 1 hour and 0 hours. The rate of non-motile spermatozoa also decreased after 2 hours compared to after 0 hours. No significant changes were observed in sperm viability (p=0.98) and non- progressive motility (p=0.48) at any time intervals. Abnormal sperm morphology increased at 1.5 hours of incubation time (p<0.001). No significant changes were observed in DNA fragmentation at 1 hour compared to 0 hours (median [interquartile range]: 19.5 [4] vs. 19 [4]), as well as at 1.5 hours compared to 1 hour (20 [5]). However, a significant increase in DNA fragmentation was observed at 1.5 hours compared to 0 hours. The mitochondrial membrane potential decreased remarkably after 1 hour of incubation time. No significant differences were observed in the acrosome reaction or malonaldehyde levels at any time point (p=0.34 and p=0.98, respectively).Conclusion: The incubation of normozoospermic samples before use in assisted reproductive technology should be less than 1.5 hours to minimize the destructive effects of prolonged incubation time on general and specific sperm parameters.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 4","pages":"270-276"},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f8/e5/cerm-2022-05435.PMC9732074.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10361117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive evaluation of embryo quality for the selection of transferable embryos in human in vitro fertilization-embryo transfer. 人类体外受精-胚胎移植中可移植胚胎选择的无创胚胎质量评价。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-12-01 DOI: 10.5653/cerm.2022.05575

Jihyun Kim, Jaewang Lee, Jin Hyun Jun

{"title":"Non-invasive evaluation of embryo quality for the selection of transferable embryos in human in vitro fertilization-embryo transfer.","authors":"Jihyun Kim, Jaewang Lee, Jin Hyun Jun","doi":"10.5653/cerm.2022.05575","DOIUrl":"https://doi.org/10.5653/cerm.2022.05575","url":null,"abstract":"The ultimate goal of human assisted reproductive technology is to achieve a healthy pregnancy and birth, ideally from the selection and transfer of a single competent embryo. Recently, techniques for efficiently evaluating the state and quality of preimplantation embryos using time-lapse imaging systems have been applied. Artificial intelligence programs based on deep learning technology and big data analysis of time-lapse monitoring system during in vitro culture of preimplantation embryos have also been rapidly developed. In addition, several molecular markers of the secretome have been successfully analyzed in spent embryo culture media, which could easily be obtained during in vitro embryo culture. It is also possible to analyze small amounts of cell-free nucleic acids, mitochondrial nucleic acids, miRNA, and long non-coding RNA derived from embryos using real-time polymerase chain reaction (PCR) or digital PCR, as well as next-generation sequencing. Various efforts are being made to use non-invasive evaluation of embryo quality (NiEEQ) to select the embryo with the best developmental competence. However, each NiEEQ method has some limitations that should be evaluated case by case. Therefore, an integrated analysis strategy fusing several NiEEQ methods should be urgently developed and confirmed by proper clinical trials.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 4","pages":"225-238"},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a8/50/cerm-2022-05575.PMC9732075.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10729946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sperm DNA fragmentation in consecutive ejaculates from patients with cancer for sperm cryopreservation. 冷冻保存癌症患者连续射精的精子DNA片段。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-09-01 Epub Date: 2022-08-31 DOI: 10.5653/cerm.2022.05323

Seul Ki Kim, Haerin Paik, Jung Ryeol Lee, Byung Chul Jee

{"title":"Sperm DNA fragmentation in consecutive ejaculates from patients with cancer for sperm cryopreservation.","authors":"Seul Ki Kim, Haerin Paik, Jung Ryeol Lee, Byung Chul Jee","doi":"10.5653/cerm.2022.05323","DOIUrl":"https://doi.org/10.5653/cerm.2022.05323","url":null,"abstract":"Objective: This prospective consecutive study investigated the variation in sperm DNA fragmentation (SDF) in multiple semen samples from patients with cancer.Methods: Eighty-one patients with various cancers underwent multiple semen collections on 3 consecutive days for sperm cryopreservation prior to cancer treatment. A commercial Halosperm kit was used to measure SDF. Within- and between-subject coefficients of variation were estimated via random-effects analysis of variance to assess the consistency of semen parameters and SDF. Intraclass correlation coefficients (ICCs) were calculated to assess the magnitude of the between-subject component of variance relative to the total variance.Results: The volume of semen in the day-2 and day-3 samples was significantly lower compared with the day-1 sample. Most parameters showed high ICC values, suggesting that within-subject fluctuations were small relative to the between-subject variability. The highest ICC values were identified for the SDF (ICC, 0.68; 95% confidence interval [CI], 0.45-0.84) and semen volume (ICC, 0.67; 95% CI, 0.45-0.84).Conclusion: Our findings showed that repeated ejaculates from patients with cancer had stable SDF levels.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 3","pages":"196-201"},"PeriodicalIF":1.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/35/dc/cerm-2022-05323.PMC9468694.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33464445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preimplantation genetic testing for aneuploidy: The management of mosaic embryos. 非整倍体的着床前基因检测:镶嵌胚胎的管理。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-09-01 Epub Date: 2022-08-31 DOI: 10.5653/cerm.2022.05393

Eun Jeong Yu, Min Jee Kim, Eun A Park, Inn Soo Kang

引用次数: 1

Effect of endometrial cell-conditioned medium and platelet-rich plasma on the developmental competence of mouse preantral follicles: An in vitro study. 子宫内膜细胞条件培养基和富血小板血浆对小鼠腔前卵泡发育能力影响的体外研究。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-09-01 DOI: 10.5653/cerm.2022.05260

Neda Taghizabet, Soghra Bahmanpour, Nehleh Zarei-Fard, Gholamreza Mohseni, Fereshteh Aliakbari, Farzaneh Dehghani

{"title":"Effect of endometrial cell-conditioned medium and platelet-rich plasma on the developmental competence of mouse preantral follicles: An in vitro study.","authors":"Neda Taghizabet, Soghra Bahmanpour, Nehleh Zarei-Fard, Gholamreza Mohseni, Fereshteh Aliakbari, Farzaneh Dehghani","doi":"10.5653/cerm.2022.05260","DOIUrl":"https://doi.org/10.5653/cerm.2022.05260","url":null,"abstract":"Objective: The aim of this study was to evaluate the impacts of platelet-rich plasma (PRP) and conditioned medium (CM) derived from endometrial stromal cells on mouse preantral follicle culture in a two-dimensional system to produce competent mature oocytes for fertilization.Methods: In total, 240 preantral follicles were isolated from female mouse ovarian tissue and divided into four groups. The preantral follicles were isolated three times for each group and then cultured, respectively, in the presence of alpha minimum essential medium (control), PRP, CM, and PRP+CM. The in vitro growth, in vitro maturation, and cleavage percentage of the preantral follicles were investigated. Immunocytochemistry (IHC) was also conducted to monitor the meiotic progression of the oocytes. Additionally, the mRNA expression levels of the two folliculogenesis-related genes (Gdf9 and Bmp15) and two apoptosis-related genes (Bcl2 and Bax) were investigated using real-time polymerase chain reaction.Results: In the PRP, CM, and PRP+CM groups, the preantral follicle maturation (evaluated by identifying polar bodies) were greater than the control group. The cleavage rate in the CM, and PRP+CM groups were also greater than the control group. IHC analysis demonstrated that in each treatment group, meiotic spindle was normal. In the PRP+CM group, the gene expression levels of Bmp15, Gdf9, and Bcl2 were greater than in the other groups. The Bax gene was more strongly expressed in the PRP and control groups than in the other groups.Conclusion: Overall, the present study suggests that the combination of CM and PRP can effectively increase the growth and cleavage rate of mouse preantral follicles in vitro.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 3","pages":"175-184"},"PeriodicalIF":1.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9b/a4/cerm-2022-05260.PMC9468696.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33464443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm DNA fragmentation negatively influences the cumulative live birth rate in the intracytoplasmic sperm injection cycles of couples with unexplained infertility. 精子DNA断裂对不明原因不育夫妇胞浆内单精子注射周期累积活产率有负面影响。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-09-01 Epub Date: 2022-08-31 DOI: 10.5653/cerm.2021.05169

Deepthi Repalle, K V Saritha, Shilpa Bhandari

{"title":"Sperm DNA fragmentation negatively influences the cumulative live birth rate in the intracytoplasmic sperm injection cycles of couples with unexplained infertility.","authors":"Deepthi Repalle, K V Saritha, Shilpa Bhandari","doi":"10.5653/cerm.2021.05169","DOIUrl":"https://doi.org/10.5653/cerm.2021.05169","url":null,"abstract":"Objective: This study aimed to determine the effect of sperm DNA fragmentation (SDF) on the cumulative live birth rate (CLBR) in intracytoplasmic sperm injection (ICSI) cycles in couples with unexplained infertility.Methods: We conducted a prospective study of 145 couples who underwent ICSI cycles for unexplained infertility. Based on the SDF rate, patients were categorized into a low SDF group (SDF ≤30%, n=97) and a high SDF group (SDF >30%, n=48). SDF was assessed using the acridine orange test on density gradient centrifugation prepared samples. The CLBR was calculated as the first live birth event per woman per egg collection over 2 years.Results: The high SDF group (SDF >30%) showed a significantly lower CLBR (p<0.05) and a significantly higher miscarriage rate (p<0.05) than the low SDF group (SDF ≤30%). No significant difference was observed in the implantation and cumulative pregnancy rates between the two SDF groups. The total number of embryo transfers was stratified further into fresh and frozen embryo transfers. In the fresh embryo transfers, there were significant differences in the implantation rates, clinical pregnancy rates, and live birth rates (p<0.05) between the low SDF and high SDF groups. However, in the frozen embryo transfers, there were no significant differences in clinical outcomes between the two groups. In the multivariable logistic regression analysis, SDF was a predictor of CLBR (p<0.05) when adjusted for possible confounding factors.Conclusion: High SDF was associated with a lower CLBR and a higher miscarriage rate in the ICSI cycles of couples with unexplained infertility.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"49 3","pages":"185-195"},"PeriodicalIF":1.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/43/ed/cerm-2021-05169.PMC9468691.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33464444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A case of congenital cloacal exstrophy/omphalocele-exstrophy-imperforate anus-spinal defects syndrome and a successful pregnancy. 先天性泄殖腔外翻/脐膨出-闭锁肛-脊柱缺陷综合征1例并成功妊娠。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2022-09-01 DOI: 10.5653/cerm.2021.05148

Mara Seat, Munira Boxwalla, Arielle Hough, Glenn Goodwin

引用次数: 1