Clinical and Experimental Reproductive Medicine-CERM最新文献

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Pathological and vascular changes in the rat testiсle after experimental trauma. 实验性创伤后大鼠睾丸的病理和血管变化
IF 1.8
Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-20 DOI: 10.5653/cerm.2024.07080
Anastasiya Spaska, Bogdan Grytsuliak, Nelia Dolynko
{"title":"Pathological and vascular changes in the rat testiсle after experimental trauma.","authors":"Anastasiya Spaska, Bogdan Grytsuliak, Nelia Dolynko","doi":"10.5653/cerm.2024.07080","DOIUrl":"https://doi.org/10.5653/cerm.2024.07080","url":null,"abstract":"<p><strong>Objective: </strong>Mechanical trauma to the testicles poses a potential risk of tissue destruction, disruption of local blood supply, and impairment of spermatogenesis, which can ultimately lead to infertility. Therefore, investigating this topic is crucial. The study aimed to identify cytological and morphological changes in the testicular tissue of laboratory rats following mechanical trauma to the organ.</p><p><strong>Methods: </strong>Observations were recorded on days 7, 14, 30, and 90 post-trauma. The experiment involved two groups of animals: a control group of healthy animals and an experimental group that sustained blunt mechanical trauma. Tissue samples were collected, fixed, dehydrated, and embedded in paraffin; subsequently, sections were prepared and stained. Structural changes in tissues and cells were documented using light and transmission electron microscopy.</p><p><strong>Results: </strong>In the experimental sample, notable changes included a decrease in organ weight, thickening of the protein shell and tubule walls, sclerotisation of the tubule membrane, narrowing of tubule diameter, reduced spermatozoa and spermatids titre, diminished capillary network and spermatogenic epithelium, uneven blood vessel lumen expansion, and decreased volume of Leydig cell nuclei. Additionally, in cells under different functional loads, the cytoplasm was vacuolated, mitochondrial cristae and the Golgi apparatus were diminished, cytoplasm volume decreased, karyopyknosis was observed, and uncharacteristic protrusions appeared on the surface of the cytoplasmic membrane. The severity of destruction at the cellular and tissue levels showed a positive correlation with time.</p><p><strong>Conclusion: </strong>The data obtained from these model sites can be predictive for clinical trials.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Does coenzyme Q10 supplementation protect spermatogenesis in ciprofloxacin-induced rat testes? 补充辅酶Q10是否能保护环丙沙星诱导的大鼠睾丸的精子发生?
IF 1.8
Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-20 DOI: 10.5653/cerm.2024.07017
Elham Aliabadi, Fateme Amooei, Tahereh Talaei Khozani, Saied Karbalay-Doust, Fatemeh Karimi
{"title":"Does coenzyme Q10 supplementation protect spermatogenesis in ciprofloxacin-induced rat testes?","authors":"Elham Aliabadi, Fateme Amooei, Tahereh Talaei Khozani, Saied Karbalay-Doust, Fatemeh Karimi","doi":"10.5653/cerm.2024.07017","DOIUrl":"https://doi.org/10.5653/cerm.2024.07017","url":null,"abstract":"<p><strong>Objective: </strong>Ciprofloxacin (CPFX) is frequently prescribed by fertility specialists and urologists to manage infections in male reproductive organs. However, it is toxic to the testicles and can lead to infertility. Dietary antioxidants are known to protect the testis from damage. This study aimed to investigate the effects of coenzyme Q10 (CoQ10) on the adverse side effects of CPFX using stereological methods.</p><p><strong>Methods: </strong>Sixty rats were divided into six groups: control (distilled water), CoQ10 (10 mg/kg/day), and low-dose (103 mg/kg/day) and high-dose (206 mg/kg/day) of CPFX (LD-CPFX, HD-CPFX) with or without CoQ10 consumption. The treatments lasted for 45 days. Sperm count, serum testosterone levels, and testicular parameters were evaluated.</p><p><strong>Results: </strong>Significant decreases in sperm count, motility, normal morphology, viability, and testosterone levels were observed in the LD-CPFX (p<0.003) and HD-CPFX- treated rats (p=0.0001) compared to the control groups. A 10% to 36% reduction in the volume of seminiferous tubules, tubular epithelium, and tubule length was noted in LD-CPFX (p<0.01) and HD-CPFX-treated rats (p<0.006), while the volume of the interstitium increased by 25% to 28% in LD-CPFX (p=0.03) and HD-CPFX (p=0.008) groups. The number of cells, including spermatogonia, spermatocytes, spermatids, Sertoli cells, and Leydig cells, decreased by 36% to 75% in the testes exposed to LD-CPFX (p<0.04) and HD-CPFX (p<0.01), compared to the control groups. However, these changes normalized in rats that received CoQ10.</p><p><strong>Conclusion: </strong>CPFX exposure for 45 days, regardless of the dose, has detrimental effects on testicular parameters. CoQ10 can prevent CPFX-induced testicular structural impairments.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
State-of-the-art in high throughput organ-on-chip for biotechnology and pharmaceuticals. 用于生物技术和制药的高通量片上器官的最新技术。
IF 1.8
Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-20 DOI: 10.5653/cerm.2024.06954
Suk-Heung Song, Sehoon Jeong
{"title":"State-of-the-art in high throughput organ-on-chip for biotechnology and pharmaceuticals.","authors":"Suk-Heung Song, Sehoon Jeong","doi":"10.5653/cerm.2024.06954","DOIUrl":"https://doi.org/10.5653/cerm.2024.06954","url":null,"abstract":"<p><p>Modern drug discovery is driven by high demand in the pharmaceutical industry to test growing libraries of compounds against potential targets. High-throughput screening (HTS) is characterized by fully automated experimentation that leverages robotic liquid handling systems, analytical techniques, and advanced computing and statistics, including the recent integration of artificial intelligence. To align with this trend, it is crucial to develop and implement new HTS platforms that offer improved predictivity and physiological relevance. In recent years, microphysiological systems, commonly known as organ-on-chip (OoC) systems, have progressed from a theoretical concept to a powerful alternative to conventional in vitro and animal models. High-throughput OoC (HT-OoC) systems could represent the disruptive technology sought by pharmaceutical companies to address their enormous research and development (R&D) expenses. In this study, we provide a brief overview of commercial products utilizing modern HT-OoC systems in drug discovery and development. Additionally, we discuss recent trends in R&D aimed at industrialization.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolomic profiling of embryo culture media in patients with repeated implantation failure during assisted reproductive technology cycles. 辅助生殖技术周期中反复植入失败患者的胚胎培养基代谢组学分析。
IF 1.8
Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-01 Epub Date: 2024-04-11 DOI: 10.5653/cerm.2023.06429
Sheyda Nami, Azam Govahi, Nabaa Najjar, Samaneh Ghasemi, Farshid Rezaei, Fatemehsadat Amjadi, Gholamreza Taheripak
{"title":"Metabolomic profiling of embryo culture media in patients with repeated implantation failure during assisted reproductive technology cycles.","authors":"Sheyda Nami, Azam Govahi, Nabaa Najjar, Samaneh Ghasemi, Farshid Rezaei, Fatemehsadat Amjadi, Gholamreza Taheripak","doi":"10.5653/cerm.2023.06429","DOIUrl":"10.5653/cerm.2023.06429","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the metabolic status of the spent culture media from embryos of patients with repeated implantation failure (RIF) undergoing in vitro fertilization-intracytoplasmic sperm injection cycles in comparison with the embryos from healthy fertile women.</p><p><strong>Methods: </strong>Metabolite levels in spent culture media were assessed and compared between embryos from RIF patients (n=35) and oocyte donors as controls (n=15). Protein levels of insulin-like growth factor 1 (IGF-1) were determined using Western blotting. Concentrations of glucose, pyruvate, and lactate were measured using spectrophotometry. Ionic colorimetric assay kits were utilized to analyze the concentrations of sodium, chloride, calcium, and magnesium ions. High-performance liquid chromatography was employed to measure the concentrations of glutamic acid, aspartic acid, methionine, phenylalanine, and histidine.</p><p><strong>Results: </strong>Glucose consumption and lactate secretion were higher in the control group than in the RIF group. The magnesium concentration was significantly higher in the control group than in the RIF group, but glutamic acid and aspartic acid concentrations were lower in the control group than in the RIF patients (p<0.05). The levels of IGF-1, sodium, calcium, chloride, methionine, histidine, and phenylalanine did not show statistically significant differences between the two groups.</p><p><strong>Conclusion: </strong>The metabolic profile of the culture medium of the embryos in the RIF group differed from that of the control group. These findings suggest potential factors that may affect implantation capacity in RIF patients and provide a new perspective on embryo selection.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"260-267"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140865190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vivo and in vitro sperm production: an overview of the challenges and advances in male fertility restoration. 体内和体外精子生成:男性生育能力恢复的挑战和进展概览。
IF 1.8
Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-01 Epub Date: 2024-03-25 DOI: 10.5653/cerm.2023.06569
Zahra Bashiri, Seyed Jamal Hosseini, Maryam Salem, Morteza Koruji
{"title":"In vivo and in vitro sperm production: an overview of the challenges and advances in male fertility restoration.","authors":"Zahra Bashiri, Seyed Jamal Hosseini, Maryam Salem, Morteza Koruji","doi":"10.5653/cerm.2023.06569","DOIUrl":"10.5653/cerm.2023.06569","url":null,"abstract":"<p><p>Male infertility can be caused by genetic anomalies, endocrine disorders, inflammation, and exposure to toxic chemicals or gonadotoxic treatments. Therefore, several recent studies have concentrated on the preservation and restoration of fertility to enhance the quality of life for affected individuals. It is currently recommended to biobank the tissue extracted from testicular biopsies to provide a later source of spermatogonial stem cells (SSCs). Another successful approach has been the in vitro production of haploid male germ cells. The capacity of SSCs to transform into sperm, as in testicular tissue transplantation, SSC therapy, and in vitro or ex vivo spermatogenesis, makes them ideal candidates for in vivo fertility restoration. The transplantation of SSCs or testicular tissue to regenerate spermatogenesis and create embryos has been achieved in nonhuman mammal species. Although the outcomes of human trials have yet to be released, this method may soon be approved for clinical use in humans. Furthermore, regenerative medicine techniques that develop tissue or cells on organic or synthetic scaffolds enriched with bioactive molecules have also gained traction. All of these methods are now in different stages of experimentation and clinical trials. However, thanks to rigorous studies on the safety and effectiveness of SSC-based reproductive treatments, some of these techniques may be clinically available in upcoming decades.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"171-180"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Outcomes of female reproductive performance with assisted reproductive techniques after recent mild to moderate COVID-19 infections: An observational study. 近期轻度至中度 COVID-19 感染后使用辅助生殖技术的女性生殖效果:一项观察性研究。
IF 1.8
Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-01 Epub Date: 2024-03-25 DOI: 10.5653/cerm.2023.06352
Ashraf Moini, Narges Najafpour, Ladan Kashani, Maryam Farid-Mojtahedi, Arezoo Maleki-Hajiagha, Afsaneh Tehranian, Rana Karimi
{"title":"Outcomes of female reproductive performance with assisted reproductive techniques after recent mild to moderate COVID-19 infections: An observational study.","authors":"Ashraf Moini, Narges Najafpour, Ladan Kashani, Maryam Farid-Mojtahedi, Arezoo Maleki-Hajiagha, Afsaneh Tehranian, Rana Karimi","doi":"10.5653/cerm.2023.06352","DOIUrl":"10.5653/cerm.2023.06352","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to evaluate the impact of preimplantation genetic testing for aneuploidy (PGT-A) on clinical outcomes among high-risk patients.</p><p><strong>Methods: </strong>This retrospective study involved 1,368 patients and the same number of cycles, including 520 cycles with PGT-A and 848 cycles without PGT-A. The study participants comprised women of advanced maternal age (AMA) and those affected by recurrent implantation failure (RIF), recurrent pregnancy loss (RPL), or severe male factor infertility (SMF).</p><p><strong>Results: </strong>PGT-A was associated with significant improvements in the implantation rate (IR) and the ongoing pregnancy rate/live birth rate (OPR/LBR) per embryo transfer cycle in the AMA (39.3% vs. 16.2% [p<0.001] and 42.0% vs. 21.8% [p<0.001], respectively), RIF (41.7% vs. 22.0% [p<0.001] and 47.0% vs. 28.6% [p<0.001], respectively), and RPL (45.6% vs. 19.5% [p<0.001] and 49.1% vs. 24.2% [p<0.001], respectively) groups, as well as the IR in the SMF group (43.3% vs. 26.5%, p=0.011). Additionally, PGT-A was associated with lower overall incidence rates of pregnancy loss in the AMA (16.7% vs. 34.3%, p=0.001) and RPL (16.7% vs. 50.0%, p<0.001) groups. However, the OPR/LBR per total cycle across all PGT-A groups did not significantly exceed that for the control groups.</p><p><strong>Conclusion: </strong>PGT-A demonstrated beneficial effects in high-risk patients. However, our findings indicate that these benefits are more pronounced in carefully selected candidates than in the entire high-risk patient population.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"268-275"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potential ameliorative effects of bilberry (Vaccinium myrtillus L.) fruit extract on cisplatin-induced reproductive damage in adult male albino rats. 山桑子(Vaccinium myrtillus L.)果实提取物对顺铂诱导的成年雄性白化大鼠生殖损伤的潜在改善作用
IF 1.8
Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-01 Epub Date: 2024-04-12 DOI: 10.5653/cerm.2023.06380
Fatma B Mossa, Nadia Bakry, Mamdouh Rashad El-Sawi
{"title":"Potential ameliorative effects of bilberry (Vaccinium myrtillus L.) fruit extract on cisplatin-induced reproductive damage in adult male albino rats.","authors":"Fatma B Mossa, Nadia Bakry, Mamdouh Rashad El-Sawi","doi":"10.5653/cerm.2023.06380","DOIUrl":"10.5653/cerm.2023.06380","url":null,"abstract":"<p><strong>Objective: </strong>Cisplatin (CP) is a widely used chemotherapeutic agent, but its severe side effects impact testicular function. We investigated the potential protective effects of bilberry extract against CP-induced testicular toxicity.</p><p><strong>Methods: </strong>Forty adult male albino rats were divided into four groups. Control animals received a single oral dose of 0.9% saline. Bilberry-treated rats received oral bilberry extract (200 mg/kg body weight [BW] dissolved in 1 mL of saline) daily for 10 consecutive days. CP-treated animals were administered a single intraperitoneal dose (7.5 mg/kg BW). Finally, a bilberry+CP group received oral bilberry extract (200 mg/kg BW) daily for 10 consecutive days, with one intraperitoneal dose of CP (7.5 mg/kg BW) on day 2. We assessed sperm count, motility, viability, and abnormalities, along with testis weight, testis weight-to-BW ratio, antioxidant activity, levels of oxidative stress markers (malondialdehyde [MDA] and hydrogen peroxide [H2O2]), sex hormones (follicle-stimulating hormone [FSH], luteinizing hormone [LH], and testosterone), and apoptotic and anti-apoptotic markers, and DNA damage. Testicular tissue underwent histopathological examination.</p><p><strong>Results: </strong>Among CP-treated rats, significantly lower values were observed for testis weight; testis weight-to-BW ratio; levels of FSH, LH, testosterone, superoxide dismutase, catalase, glutathione S-transferase, glutathione, and B-cell lymphoma 2; and sperm count, motility, and proportion of normal sperm. CP administration was associated with higher MDA, H2O2, p53, Bax, cytochrome c, caspase 9, and caspase 3 levels, along with elevated tail moment. However, bilberry extract administration significantly improved all altered parameters.</p><p><strong>Conclusion: </strong>Bilberry treatment demonstrated protective effects and reduced CP-induced testicular toxicity via antioxidant activity and cytoprotection.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"51 3","pages":"192-204"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective effect of platelet-rich plasma against structural and functional changes of the adult rat testis in carbimazole-induced hypothyroidism. 富血小板血浆对卡比马唑诱发甲状腺功能减退症的成年大鼠睾丸结构和功能变化的保护作用
IF 1.8
Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-01 Epub Date: 2024-04-11 DOI: 10.5653/cerm.2023.06695
Hossein Bordbar, Masoud Sattar-Shamsabadi, Farzaneh Dehghani, Fatemeh Karimi
{"title":"Protective effect of platelet-rich plasma against structural and functional changes of the adult rat testis in carbimazole-induced hypothyroidism.","authors":"Hossein Bordbar, Masoud Sattar-Shamsabadi, Farzaneh Dehghani, Fatemeh Karimi","doi":"10.5653/cerm.2023.06695","DOIUrl":"10.5653/cerm.2023.06695","url":null,"abstract":"<p><strong>Objective: </strong>Hypothyroidism (HT) influences spermatogenesis and is associated with male infertility. Platelet-rich plasma (PRP), a biological product rich in growth factors, promotes tissue repair. In this study, the likely protective effects of PRP on testicular tissue damage in carbimazole (CBZ)-induced HT were evaluated.</p><p><strong>Methods: </strong>Forty male rats were divided into four groups. HT was induced by administering CBZ (1.35 mg/kg orally, for 45 days). Two doses of PRP (40 μL each, locally injected into the testis on days 15 and 30) were also given. After 45 days, blood samples were taken from the heart to measure triiodothyronine (T3), thyroxine (T4), and testosterone levels, and semen analysis was performed. For stereological assessment, the left testis was removed, fixed, embedded, sectioned, and stained with hematoxylin and eosin. The right testis was excised to evaluate antioxidant levels.</p><p><strong>Results: </strong>CBZ was demonstrated to induce HT, characterized by significant reductions in T3 and T4. HT was associated with decreased testicular weight, impaired sperm parameters, reduced testosterone concentration, diminished antioxidant activity, reduced volumes of testicular components, and lower total numbers of testicular cells of various types. When HT samples were treated with PRP, improvement was observed for all of these changes. This protective effect could be attributed to the growth factors present in PRP.</p><p><strong>Conclusion: </strong>PRP appears to prevent the structural changes in the testes and the deterioration in sperm quality caused by CBZ-induced HT. This protective effect is likely due to mitigation of oxidative damage and elevation of testosterone levels.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"225-235"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140871510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effects of vitamin E supplementation on sperm parameters, chromatin integrity, and gene expression before and after freezing in aged mice. 补充维生素 E 对老年小鼠冷冻前后精子参数、染色质完整性和基因表达的影响。
IF 1.8
Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-01 Epub Date: 2024-06-10 DOI: 10.5653/cerm.2023.06632
Sadegh Zarei, Farnoosh Molavi, Farzaneh Abbas Abasnezhad, Behanaz Majidi, Saeed Mohammadihosseinabad, Faezeh Esmaeili Ranjbar, Mahboubeh Vatanparast
{"title":"The effects of vitamin E supplementation on sperm parameters, chromatin integrity, and gene expression before and after freezing in aged mice.","authors":"Sadegh Zarei, Farnoosh Molavi, Farzaneh Abbas Abasnezhad, Behanaz Majidi, Saeed Mohammadihosseinabad, Faezeh Esmaeili Ranjbar, Mahboubeh Vatanparast","doi":"10.5653/cerm.2023.06632","DOIUrl":"10.5653/cerm.2023.06632","url":null,"abstract":"<p><strong>Objective: </strong>Some age-related testicular changes, such as Sertoli cell vacuolization and blood-testis barrier breakdown, reduce total sperm production and male fertility. Therefore, this study investigated the effect of vitamin E on restoring testicular function in aged mice. Sperm cryo-resistance was also assessed.</p><p><strong>Methods: </strong>Twenty-eight 48-week-old male Naval Medical Research Institute mice were divided into four groups for a daily gavage of vitamin E: the control group received distilled water, while the three treatment groups were administered 100, 200, and 400 mg/kg, respectively, for 4 weeks. Subsequently, semen analyses, DNA fragmentation index (DFI), and protamine deficiency tests were conducted. Testicular histology, tissue antioxidant enzyme activity, and gene expression levels were also assessed.</p><p><strong>Results: </strong>The two higher dosages of vitamin E were associated with a higher sperm count, greater progressive motility, and improved sperm morphology (p<0.05). These benefits were also evident after sperm freezing (p<0.05). Although chromatin abnormalities increased following vitrification, the treatment groups showed better outcomes (p<0.05). The tubular diameter, epithelium height, and luminal diameters remained unchanged with age. The tissue antioxidant capacity was greater in the groups receiving the high doses of vitamin E. Additionally, significant increases in inhibitor of DNA binding protein-4 (Id4) and GDNF family receptor alpha-1 (Gfra1) expression were observed in the higher vitamin E dosage groups, and promyelocytic leukemia zinc finger protein (Plzf) expression was notably present in the 400 mg/kg treatment group compared to the control group (p<0.05).</p><p><strong>Conclusion: </strong>Antioxidant supplementation might enhance reproductive outcomes in aging males. The observed effects included improved sperm cryo-resistance, which is advantageous for future applications such as sperm freezing or fertility preservation.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"213-224"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective effects of Withania somnifera against cyclophosphamide-induced testicular damage in rats. 睡茄对环磷酰胺引起的大鼠睾丸损伤的保护作用
IF 1.8
Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-01 Epub Date: 2024-06-10 DOI: 10.5653/cerm.2023.06415
Mehrana Jafari, Ahmad Akbari, Zeynab Esmailpour, Zahra Nadi, Maryam Baazm
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