{"title":"Clinical pregnancy rates after two different methods of laser-assisted hatching applied to vitrified-warmed day-3 embryos or day-5 blastocysts.","authors":"Woo Jeong Kim, Byung Chul Jee","doi":"10.5653/cerm.2024.07073","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Laser-assisted hatching (LAH) employs two distinct techniques: thinning and breaching. This study aimed to compare the clinical efficacy of combined thinning and breaching versus breaching alone in vitrified-warmed embryo or blastocyst transfer cycles.</p><p><strong>Methods: </strong>In total, 110 vitrified-warmed day-3 embryo transfer cycles and 50 vitrified-warmed day-5 blastocyst transfer cycles were retrospectively selected. All transfers were performed between 2021 and 2022 at a university-based infertility center. Combined thinning and breaching involved thinning either one-quarter or one-sixth of the zona pellucida circumference combined with breaching at a single point.</p><p><strong>Results: </strong>In vitrified-warmed day-3 embryo transfer cycles, the 'thinning and breaching' and 'breaching only' groups were similar regarding the median age of the woman (36 years vs. 37 years, respectively), number of embryos transferred (2 vs. 2), and embryo score (89 vs. 31.5). The clinical pregnancy rate (PR) (23.5% vs. 21.1%), embryo implantation rate (IR) (11.4% vs. 11.3%), and clinical miscarriage rate (25% vs. 37.5%) were also comparable between the two LAH groups. In vitrified-warmed day-5 blastocyst transfer cycles, the combination and breaching-only groups were similar in the median age of the woman (36 years vs. 36.5 years, respectively), number of blastocysts transferred (1 vs. 1), and blastocyst score (45 vs. 31.5). The clinical PR (45.5% vs. 42.9%), IR (35.4% vs. 33.3%), and clinical miscarriage rate (20% vs. 33.3%) were also similar between groups.</p><p><strong>Conclusion: </strong>Breaching alone displayed similar efficacy to combined thinning and breaching in terms of clinical PR, IR, and miscarriage rate. Thus, breaching alone appears sufficient to achieve favorable pregnancy outcomes.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Reproductive Medicine-CERM","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5653/cerm.2024.07073","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: Laser-assisted hatching (LAH) employs two distinct techniques: thinning and breaching. This study aimed to compare the clinical efficacy of combined thinning and breaching versus breaching alone in vitrified-warmed embryo or blastocyst transfer cycles.
Methods: In total, 110 vitrified-warmed day-3 embryo transfer cycles and 50 vitrified-warmed day-5 blastocyst transfer cycles were retrospectively selected. All transfers were performed between 2021 and 2022 at a university-based infertility center. Combined thinning and breaching involved thinning either one-quarter or one-sixth of the zona pellucida circumference combined with breaching at a single point.
Results: In vitrified-warmed day-3 embryo transfer cycles, the 'thinning and breaching' and 'breaching only' groups were similar regarding the median age of the woman (36 years vs. 37 years, respectively), number of embryos transferred (2 vs. 2), and embryo score (89 vs. 31.5). The clinical pregnancy rate (PR) (23.5% vs. 21.1%), embryo implantation rate (IR) (11.4% vs. 11.3%), and clinical miscarriage rate (25% vs. 37.5%) were also comparable between the two LAH groups. In vitrified-warmed day-5 blastocyst transfer cycles, the combination and breaching-only groups were similar in the median age of the woman (36 years vs. 36.5 years, respectively), number of blastocysts transferred (1 vs. 1), and blastocyst score (45 vs. 31.5). The clinical PR (45.5% vs. 42.9%), IR (35.4% vs. 33.3%), and clinical miscarriage rate (20% vs. 33.3%) were also similar between groups.
Conclusion: Breaching alone displayed similar efficacy to combined thinning and breaching in terms of clinical PR, IR, and miscarriage rate. Thus, breaching alone appears sufficient to achieve favorable pregnancy outcomes.
目的:激光辅助孵化(LAH)采用两种不同的技术:细化和突破。本研究旨在比较在玻璃化加热胚胎或囊胚移植周期中联合稀释和破裂与单独破裂的临床疗效。方法:回顾性选择110个玻璃化加热第3天胚胎移植周期和50个玻璃化加热第5天囊胚移植周期。所有转移都是在2021年至2022年期间在一所大学的不孕不育中心进行的。联合减薄和破坏是指将透明带周长的四分之一或六分之一减薄并在一个点上进行破坏。结果:在玻璃化加热的第3天胚胎移植周期中,“变薄和破裂”组和“仅破裂”组在女性的中位年龄(分别为36岁对37岁)、移植的胚胎数量(2对2)和胚胎评分(89对31.5)方面相似。两组临床妊娠率(PR) (23.5% vs. 21.1%)、胚胎着床率(IR) (11.4% vs. 11.3%)、临床流产率(25% vs. 37.5%)也具有可比性。在玻璃化加热的第5天囊胚移植周期中,联合组和单独破碎组在女性的中位年龄(分别为36岁和36.5岁)、移植囊胚数量(1比1)和囊胚评分(45比31.5)方面相似。临床PR (45.5% vs. 42.9%)、IR (35.4% vs. 33.3%)和临床流产率(20% vs. 33.3%)组间也相似。结论:在临床PR、IR和流产率方面,单独破壁与联合破壁疗效相近。因此,单独破裂似乎足以获得良好的妊娠结局。