Clinical and Experimental Reproductive Medicine-CERM最新文献_第2页

The role of granulocyte-macrophage colony-stimulating factor in inducing autophagy in the spermatozoa of patients with asthenoteratozoospermia. 粒细胞-巨噬细胞集落刺激因子在弱无畸形精子症患者精子诱导自噬中的作用。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-06 DOI: 10.5653/cerm.2024.07479

Tahereh Gheliji, Mohammad Hosain Haidari, Marefat Ghaffari Novin, Zahra Shams Mofarahe, Mahsa Kazemi, Latif Gachkar, Pourya Raee, Bahareh Karimi, Hamid Nazarian

{"title":"The role of granulocyte-macrophage colony-stimulating factor in inducing autophagy in the spermatozoa of patients with asthenoteratozoospermia.","authors":"Tahereh Gheliji, Mohammad Hosain Haidari, Marefat Ghaffari Novin, Zahra Shams Mofarahe, Mahsa Kazemi, Latif Gachkar, Pourya Raee, Bahareh Karimi, Hamid Nazarian","doi":"10.5653/cerm.2024.07479","DOIUrl":"https://doi.org/10.5653/cerm.2024.07479","url":null,"abstract":"Objective: The aim of this study is to investigate the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) treatment on the autophagy process and sperm parameters in individuals with asthenoteratozoospermia.Methods: Twenty semen samples from patients diagnosed with asthenoteratozoospermia were divided into control and treatment groups. Subsequently, 2 ng/mL of GM-CSF was added to the treatment group samples. All samples were then incubated for 1 hour. Post-incubation, the protein levels of light chain 3 II (LC3-II)/LC3-I and autophagy related 7 (Atg7), which are well-known autophagy markers, along with sperm motility, viability, and sperm DNA fragmentation, were analyzed in both study groups.Results: Our study demonstrated significant increases in LC3-II/LC3-I and Atg7 levels, as well as in sperm motility, in the GM-CSF group compared to the control group (p<0.0001). Furthermore, GM-CSF treatment significantly reduced necrotic cell death in the GM-CSF group relative to the control group (p<0.01). There were no significant differences between the groups in terms of sperm viability and DNA fragmentation (p>0.05).Conclusion: These results revealed that GM-CSF has the potential to significantly induce autophagy in sperm and enhance sperm motility in patients with asthenoteratozoospermia, without adversely affecting sperm viability and DNA integrity. These findings suggest that modifying autophagy with physiological and safe components like GM-CSF may become a promising therapeutic strategy for treating male infertility in the near future.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between thyroid-stimulating hormone levels and the severity of vitamin D deficiency by age group. 各年龄组促甲状腺激素水平与维生素 D 缺乏症严重程度之间的关系。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-08-19 DOI: 10.5653/cerm.2023.06779

Mansi Modi, Pinky Garg

{"title":"Relationship between thyroid-stimulating hormone levels and the severity of vitamin D deficiency by age group.","authors":"Mansi Modi, Pinky Garg","doi":"10.5653/cerm.2023.06779","DOIUrl":"10.5653/cerm.2023.06779","url":null,"abstract":"Objective: Researchers have long been captivated by the complex molecular interactions between vitamin D and the thyroid gland. Hypothyroidism affects 2% to 4% of women of reproductive age and can impact fertility through anovulatory cycles, luteal phase defects, hyperprolactinemia, and sex hormone imbalances. This study investigated the relationship between thyroid disease and the severity of vitamin D deficiency across different age groups.Methods: A retrospective study was conducted of 286 patient samples from individuals aged 18 to 60 years who were processed in the clinical biochemistry laboratory of our hospital. Samples were tested for thyroid-stimulating hormone (TSH) and vitamin D (specifically, vitamin D3) levels. The study samples were categorized into four clinically relevant groups based on TSH levels and into three groups based on serum 25-hydroxyvitamin D (25(OH)D) levels.Results: Most of the samples were from female patients (n=269), and the most common age group was 18 to 35 years (n=191, 66.78%). Subclinical hypothyroidism was identified in 120 patients, while vitamin D deficiency was present in 237 (82.87%) participants. A significant association was observed between vitamin D deficiency and the presence of thyroid disorders. Additionally, a significant negative correlation was found between TSH and vitamin D levels. Polycystic ovary syndrome was noted in 103 female patients (36.01%).Conclusion: TSH and 25(OH)D levels should be screened in all women of reproductive age, not just those in high-risk groups, as subclinical and occult hypothyroidism may otherwise go undiagnosed. Furthermore, TSH should be considered the primary screening test.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"71-78"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations of dietary inflammatory indices (DII and E-DII) with sperm parameters. 膳食炎症指数（DII 和 E-DII）与精子参数的关系。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-08-01 DOI: 10.5653/cerm.2024.06982

Sonia Sadeghpour, Fatemeh Maleki Sedgi, Sevana Daneghian, Somayyeh Barania Adabi, Tahereh Behroozi-Lak, Mohammadreza Pashaei, Javad RasouIi, Rohollah Valizadeh, Hojat Ghasemnejad-Berenji

{"title":"Associations of dietary inflammatory indices (DII and E-DII) with sperm parameters.","authors":"Sonia Sadeghpour, Fatemeh Maleki Sedgi, Sevana Daneghian, Somayyeh Barania Adabi, Tahereh Behroozi-Lak, Mohammadreza Pashaei, Javad RasouIi, Rohollah Valizadeh, Hojat Ghasemnejad-Berenji","doi":"10.5653/cerm.2024.06982","DOIUrl":"10.5653/cerm.2024.06982","url":null,"abstract":"Objective: This study aimed to explore the ambiguous link between dietary inflammatory indices and sperm parameters. Specifically, it investigated the associations between the dietary inflammatory index (DII) and the energy-adjusted dietary inflammatory index (E-DII) with sperm motility, morphology, and count in men undergoing routine semen analysis.Methods: A cross-sectional study was conducted with 144 men enrolled, where semen samples were collected and evaluated according to the 2010 World Health Organization guidelines. Dietary data were gathered using a 147-item semi-quantitative food frequency questionnaire developed by the researchers. Pearson correlation analysis was employed to assess the relationships of the DII and E-DII with sperm parameters.Results: The mean DII and E-DII scores were 1.23±1.1 and 0.49±0.43, respectively. The mean values for sperm motility, morphology, and count were 43.08%±19.30%, 78.03%±26.99%, and 48.12±44.41 million, respectively. Both motility (r=-0.353) and count (r=-0.348) were found to be inversely and significantly correlated with DII. Similarly, Pearson correlation tests revealed strong and significant inverse correlations of motility (r=-0.389) and count (r=-0.372) with E-DII.Conclusion: The findings suggest that a diet with a higher anti-inflammatory potential may be associated with increased sperm count and motility, but not with changes in morphology. Further research is necessary to confirm these findings, elucidate the underlying mechanisms, and identify dietary modifications that could improve male fertility.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"79-86"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of anxiety, depression, and sexual dysfunction in women undergoing fertility treatment. 接受生育治疗的妇女的焦虑、抑郁和性功能障碍的评估。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2025-02-28 DOI: 10.5653/cerm.2024.07465

Joon Cheol Park

{"title":"Assessment of anxiety, depression, and sexual dysfunction in women undergoing fertility treatment.","authors":"Joon Cheol Park","doi":"10.5653/cerm.2024.07465","DOIUrl":"10.5653/cerm.2024.07465","url":null,"abstract":"Objective: Infertility and fertility treatment can lead to considerable physical, emotional, and psychological distress. These effects may be influenced by personality, familial bonds, and support systems within a sociocultural context. This preliminary investigation examined anxiety, depression, and sexual dysfunction in Korean women undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI).Methods: Women scheduled for IVF/ICSI and healthy women seeking general health screening (controls) were enrolled. Participants were asked to complete standardized, validated questionnaires-the Hospital Anxiety and Depression Scale (HADS), Depression Anxiety and Stress Scale (DASS), and Female Sexual Function Index (FSFI)-to assess their levels of anxiety, depression, and sexual function.Results: The mean HADS scores for women undergoing IVF/ICSI were 6.35±3.48 (range, 0 to 13) for anxiety and 8.32±3.78 (range, 2 to 15) for depression. However, 12.9% of the women with infertility experienced clinically significant anxiety (HADS-Anxiety score >11), while 32.3% exhibited depression (HADS-Depression score >11). Based on DASS scores, psychological difficulties were more prevalent among women experiencing infertility than among control participants. Total FSFI scores, along with subscale ratings for desire, arousal, lubrication, satisfaction, and pain, were similar between women with infertility and control women. However, the mean score for orgasm was significantly lower in the infertility group (3.16) than among controls. Age, the durations of marriage and infertility, and parity did not significantly influence HADS, DASS, or FSFI scores.Conclusion: Anxiety and depression were more prevalent among women seeking fertility treatment than among healthy controls. Additionally, women with infertility may experience fewer or diminished orgasms.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"52 1","pages":"38-43"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum miR-329-3p as a potential biomarker for poor ovarian response in an in vitro fertilization. 血清miR-329-3p作为体外受精卵巢不良反应的潜在生物标志物。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2025-01-21 DOI: 10.5653/cerm.2024.07094

Jung Hoon Kim, Hye-Ok Kim, Su-Yeon Lee, Eun-A Park, Kyoung Hee Choi, Kiye Kang, Eun Jeong Yu, Mi Kyoung Koong, Kyung-Ah Lee

{"title":"Serum miR-329-3p as a potential biomarker for poor ovarian response in an in vitro fertilization.","authors":"Jung Hoon Kim, Hye-Ok Kim, Su-Yeon Lee, Eun-A Park, Kyoung Hee Choi, Kiye Kang, Eun Jeong Yu, Mi Kyoung Koong, Kyung-Ah Lee","doi":"10.5653/cerm.2024.07094","DOIUrl":"10.5653/cerm.2024.07094","url":null,"abstract":"Objective: Several miRNAs have been identified as differentially expressed in patients with poor ovarian response (POR) compared to those with normal responses. This study aims to assess the potential of serum miR-329-3p as a biomarker for diagnosing POR.Methods: We conducted a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis to confirm the target genes of miR-329-3p. KGN cells were transfected with both miR-329-3p mimic and inhibitor to assess the differential expression of these target genes. In accordance with the Bologna criteria, we enrolled 16 control patients and 16 patients with POR. We collected patient samples, including serum from day 2 and the human chorionic gonadotropin (hCG) day, as well as granulosa and cumulus cells, to validate the expression of miR-329-3p using quantitative real-time polymerase chain reaction.Results: KEGG pathway analysis revealed that miR-329-3p targeted adenylyl cyclase 9 (ADCY9) and protein kinase A subunit beta (PRKACB), both of which are involved in ovarian steroidogenesis. In KGN cells treated with a miR-329-3p mimic, ADCY9 and PRKACB expression levels were significantly reduced (p<0.05). Elevated levels of miR-329-3p suppressed aromatase expression and 17β-estradiol production by modulating ADCY9 and PRKACB in KGN cells. These effects were also observed in POR patients. Follicle-stimulating hormone receptor (FSHR) expression was diminished in the granulosa cells of POR patients. On day 2, on hCG day, and in granulosa cells, miR-329-3p exhibited high expression levels in the serum of POR patients.Conclusion: miR-329-3p exhibited increased expression in granulosa cells and in the sera of POR patients. Consequently, we propose that miR-329-3p may be a potential biomarker for the diagnosis of POR.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"52 1","pages":"44-55"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced strategies for single embryo selection in assisted human reproduction: A review of clinical practice and research methods. 人类辅助生殖中单胚胎选择的先进策略：临床实践和研究方法综述。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-06-10 DOI: 10.5653/cerm.2023.06478

Zahra Bashiri, Azita Afzali, Morteza Koruji, Hossein Torkashvand, Mehrdad Ghorbanlou, Nadia Sheibak, Zahra Zandieh, Fatemehsadat Amjadi

{"title":"Advanced strategies for single embryo selection in assisted human reproduction: A review of clinical practice and research methods.","authors":"Zahra Bashiri, Azita Afzali, Morteza Koruji, Hossein Torkashvand, Mehrdad Ghorbanlou, Nadia Sheibak, Zahra Zandieh, Fatemehsadat Amjadi","doi":"10.5653/cerm.2023.06478","DOIUrl":"10.5653/cerm.2023.06478","url":null,"abstract":"Among the primary objectives of contemporary assisted reproductive technology research are achieving the births of healthy singletons and improving overall fertility outcomes. Substantial advances have been made in refining the selection of single embryos for transfer, with the aim of maximizing the likelihood of successful implantation. The principal criterion for this selection is embryo morphology. Morphological evaluation systems are based on traditional parameters, including cell count and fragmentation, pronuclear morphology, cleavage rate, blastocyst formation, and various sequential embryonic assessments. To reduce the incidence of multiple pregnancies and to identify the single embryo with the highest potential for growth, invasive techniques such as preimplantation genetic screening are employed in in vitro fertilization clinics. However, new approaches have been suggested for clinical application that do not harm the embryo and that provide consistent, accurate results. Noninvasive technologies, such as time-lapse imaging and omics, leverage morphokinetic parameters and the byproducts of embryo metabolism, respectively, to identify noninvasive prognostic markers for competent single embryo selection. While these technologies have garnered considerable interest in the research community, they are not incorporated into routine clinical practice and still have substantial room for improvement. Currently, the most promising strategies involve integrating multiple methodologies, which together are anticipated to increase the likelihood of successful pregnancy.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"8-29"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Severe ovarian hyperstimulation syndrome in an oocyte donor despite preventive strategies. 尽管采取了预防策略，一名卵母细胞捐献者仍患上了严重的卵巢过度刺激综合征。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-07-22 DOI: 10.5653/cerm.2024.06884

Antonio Forgiarini, Mariana Selene Paredes Contreras, Silvia Bontá, Sara Maggi, Luis Alberto Quintero Espinel

引用次数: 0

Low-air-pressure clean room system: A flexible, high-quality model for assisted reproduction laboratories. 低压无尘室系统：一个灵活的，高质量的模型辅助生殖实验室。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-06-13 DOI: 10.5653/cerm.2024.06793

Minh Tam Le, Trung Van Nguyen, Thai Thanh Thi Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen

{"title":"Low-air-pressure clean room system: A flexible, high-quality model for assisted reproduction laboratories.","authors":"Minh Tam Le, Trung Van Nguyen, Thai Thanh Thi Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen","doi":"10.5653/cerm.2024.06793","DOIUrl":"10.5653/cerm.2024.06793","url":null,"abstract":"Objective: This study aimed to develop a configurable clean room paradigm with low air pressure for assisted reproductive technology (ART) clinics and demonstrate the concept's efficacy using in vitro fertilization (IVF) treatment.Methods: A high-standard clean room system with positive pressure (13 Pa) was built using accessible materials and equipment for ART laboratories. Methods for controlling and evaluating the clean room's characteristics were developed and implemented for quality assessment and calibration to maximize efficiency. The feasibility of the flexible clean room concept was assessed by analyzing the key performance indicators of embryo culture and IVF treatment.Results: After 3 weeks of testing, the concentration of particles ≥0.5 μm was 6.04 times lower than the International Organization for Standardization (ISO) class 5 standard (3,520 particles/m3) in the IVF laboratory. Air pressure, noise, temperature, and humidity were controlled stably and appropriately. Five days after installation and handover, the volatile organic compound concentration dropped to 0.00 ppm. With blastocysts and a respectable blastocyst rate, embryonic culture with female patients younger than 40 matched the criteria (63.5% and 38.9%, respectively). After vitrified blastocysts were transferred, the pregnancy and implantation rates were 58.5% and 36.2%, respectively, demonstrating a high degree of treatment success.Conclusion: Our customizable, high-quality, low-air-pressure clean room model can be implemented to achieve positive outcomes for infertility treatment.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"52 1","pages":"56-64"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preservation of ovarian function using human pluripotent stem cell-derived mesenchymal progenitor cells. 利用源自人类多能干细胞的间充质祖细胞保护卵巢功能。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-05-24 DOI: 10.5653/cerm.2024.07003

Dong Ryul Lee, Jeoung Eun Lee

{"title":"Preservation of ovarian function using human pluripotent stem cell-derived mesenchymal progenitor cells.","authors":"Dong Ryul Lee, Jeoung Eun Lee","doi":"10.5653/cerm.2024.07003","DOIUrl":"10.5653/cerm.2024.07003","url":null,"abstract":"Ovarian reserve diminishes with age, and older women experience a corresponding shift in sex hormone levels. These changes contribute to an age-dependent decrease in fertility and a decline in overall health. Furthermore, while survival rates following cancer treatment have improved for young female patients, a reduction in ovarian function due to the side effects of such treatments can be difficult to avoid. To date, no effective therapy has been recommended to preserve ovarian health in these patients. Mesenchymal progenitor cells (MPCs) are considered a promising option for cell therapy aimed at maintaining fertility and fecundity. Although MPCs derived from human adult tissues are recognized for their various protective effects against ovarian senescence, they are limited in quantity. Consequently, human pluripotent stem cell-derived MPCs (hPSC-MPCs), which exhibit high proliferative capacity and retain genetic stability during growth, have been utilized to delay reproductive aging. This review highlights the impact of hPSC-MPCs on preserving the functionality of damaged ovaries in female mouse models subjected to chemotherapy and natural aging. It also proposes their potential as a valuable cell source for fertility preservation in women with a variety of diseases.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"30-37"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal exposure to phenanthrene induces testicular apoptosis and Sertoli cell dysfunction in F1 adult male mice: a histological and molecular study. 母体暴露于菲会诱发 F1 成年雄性小鼠睾丸凋亡和 Sertoli 细胞功能障碍：一项组织学和分子研究。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2025-03-01 Epub Date: 2024-07-22 DOI: 10.5653/cerm.2024.07038

Azar Afshar, Hamid Nazarian, Fatemeh Fadaefathabadi, Fakhroddin Aghajanpour, Reza Soltani, Mohammad-Amin Abdollahifar, Gholamreza Hassanzadeh, Mohsen Nourozian

{"title":"Maternal exposure to phenanthrene induces testicular apoptosis and Sertoli cell dysfunction in F1 adult male mice: a histological and molecular study.","authors":"Azar Afshar, Hamid Nazarian, Fatemeh Fadaefathabadi, Fakhroddin Aghajanpour, Reza Soltani, Mohammad-Amin Abdollahifar, Gholamreza Hassanzadeh, Mohsen Nourozian","doi":"10.5653/cerm.2024.07038","DOIUrl":"10.5653/cerm.2024.07038","url":null,"abstract":"Objective: Phenanthrene, a polycyclic aromatic hydrocarbon, is found in abundance in environmental pollutants, food, and drinking water. This substance can accumulate in body tissues and exert harmful effects. Moreover, phenanthrene can cross the placental barrier, potentially impacting fetal development. We aimed to explore the impacts of maternal exposure to phenanthrene on testicular tissue and Sertoli cell function in F1 mice.Methods: Female rats with vaginal plugs were randomly assigned to one of three groups: control, sham, or phenanthrene. The control group received no intervention during pregnancy. In the sham and phenanthrene groups, corn oil and a phenanthrene solution, respectively, were administered via gavage once every 2 days. Offspring were separated by sex 21 days after birth. At 56 days postnatal, male F1 offspring were euthanized, and their testes were harvested for histological and molecular analyses.Results: Phenanthrene exposure was associated with a lower testicular weight and volume, a smaller diameter of the seminiferous tubules, and a relative thinning of the germinal epithelium. These changes were associated with increased cellular apoptosis, as shown by the upregulation of caspase 3 expression. Additionally, we observed an increase in vacuolization and residual bodies within the tissue. Conversely, the number of Sertoli cells and expression levels of Sox9, as well as the Ocln and Itgb1 genes, were found to be lowered.Conclusion: Maternal exposure to phenanthrene impacts both germ cells and Sertoli cells, disrupting their function and leading to fertility disorders in male F1 offspring mice.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"87-97"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0