光生物调节比长期阴囊热疗更有效地改善布苏芬诱导的无精子症小鼠的睾丸组织和精子发生。

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Fakhredin Aqajanpor, Bahman Jalali Kondori, Mohammad Hossein Asadi, Mehdi Raei, Maryam Ghasem Nezhadian, Hossein Bahadoran
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引用次数: 0

摘要

目的:光生物调节(PBM)在组织修复中的应用已被广泛接受。本研究调查并比较了PBM对暴露于阴囊高温和丁硫凡的小鼠的影响。方法:48只8周龄成年小鼠分为5组:1)对照组,2)热疗组,3)热疗组,4)热疗+PBM组,5)热疗+PBM组。为了诱导II组和IV组小鼠无精子症,每隔一天将小鼠阴囊暴露在43°C的水中,持续5周。III组和V组腹腔注射单剂量(45 mg/kg)丁硫丹。IV组和V组小鼠每隔一天接受0.03 J/cm2/sec激光照射,连续35 d。结果:分子数据分析显示,与热疗+PBM组相比,巴苏凡+PBM组的谷胱甘肽水平、增殖细胞核抗原(Pcna)表达及视黄酸基因8 (Stra8)基因刺激下的谷胱甘肽水平显著升高。此外,busulfan+PBM组的睾丸细胞数量、组织体积和精子参数也明显较高。此外,该组血清睾酮水平显著升高。结论:在阴囊高温和注射丁硫凡诱导下,激光治疗通过减少活性氧的形成和增加有丝分裂基因的表达来增强睾丸功能和精子发生。然而,在busulfan+PBM组,PBM的有效性更大。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Photobiomodulation is more effective than long-term scrotal hyperthermia in improving testis tissue and spermatogenesis in mice with busulfan-induced azoospermia.

Objective: The use of photobiomodulation (PBM) for tissue repair has gained acceptance. This study investigated and compared the effects of PBM on mice exposed to scrotal hyperthermia and busulfan.

Methods: Forty 8-week-old adult mice were divided into five groups: I) control, II) hyperthermia, III) busulfan, IV) hyperthermia+PBM, and V) busulfan+PBM. To induce azoospermia in groups II and IV, the scrotum of the mice was exposed to water at 43 °C every other day for 5 weeks. In groups III and V, a single dose of busulfan (45 mg/kg) was administered intraperitoneally. Mice in groups IV and V received laser irradiation (0.03 J/cm2/sec) every other day for 35 days.

Results: Molecular data analysis revealed that the levels of glutathione and the expression of proliferating cell nuclear antigen (Pcna) and stimulated by retinoic acid gene 8 (Stra8) genes were significantly higher in the busulfan+PBM group than in the hyperthermia+PBM group. Additionally, the number of testicular cells, tissue volume, and sperm parameters were also markedly higher in the busulfan+PBM group. Furthermore, this group exhibited a notable increase in serum testosterone levels.

Conclusion: The results demonstrated that laser therapy enhances testicular function and spermatogenesis by reducing the formation of reactive oxygen species and increasing the expression of mitotic genes following the induction of scrotal hyperthermia and busulfan injection. However, the effectiveness of PBM was greater in the busulfan+PBM group.

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