不同精液质量组的氧化应激及其与精子参数的关系。

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Maroua Ben Rhouma, Hatem Bahri, Mustapha Ben Khalifa, Mohsen Sakly, Khémais Ben Rhouma, Moncef Benkhalifa, Olfa Tebourbi
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引用次数: 0

摘要

目的:探讨氧化应激对不育男性精子质量的影响,重点研究精液中脂质过氧化和抗氧化酶——过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)的活性。方法:本研究于2021年1月至2023年1月进行,纳入163例男性不育症患者一年以上。参与者根据精液质量进行分类。按照世界卫生组织的指导方针,对精液样本进行精子浓度、活力和形态分析。通过测量丙二醛(MDA)水平(脂质过氧化指标)以及CAT、SOD和GPx的活性来评估氧化应激。获得所有参与者的伦理批准和知情同意。结果:对男性不育症患者的精液质量和氧化应激进行了评价,将患者分为五组:无精子症、少精子症、弱精子症、畸形精子症和少-弱-畸形精子症。病理组表现出精子数量、活力和形态的显著减少。此外,脂质过氧化,如MDA水平升高所示,在所有病理组中均显著升高。CAT、SOD和GPx活性在这些组中均显著降低,其中以少弱畸形精子症组下降最为明显。结论:以MDA水平升高为指标的氧化应激与精子质量差有关。病理精液中抗氧化酶活性的降低表明抗氧化防御能力的减弱导致了精子功能障碍。这些发现表明,抗氧化剂干预可以改善不育男性的精子质量,尽管还需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oxidative stress and its correlation with sperm parameters in different semen quality groups.

Objective: This study investigated oxidative stress and its impact on sperm quality in men with infertility, focusing on lipid peroxidation and the activity of antioxidant enzymes-catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)-in seminal fluid.

Methods: This study was conducted from January 2021 to January 2023 and involved 163 male patients who had been experiencing infertility for over a year. The participants were categorized according to semen quality. Semen samples were analyzed for sperm concentration, motility, and morphology following the World Health Organization guidelines. Oxidative stress was evaluated by measuring levels of malondialdehyde (MDA), an indicator of lipid peroxidation, as well as the activity of CAT, SOD, and GPx. Ethical approval and informed consent were obtained from all participants.

Results: Semen quality and oxidative stress were evaluated in cases of male infertility, with patients categorized into five groups: normozoospermia, oligozoospermia, asthenozoospermia, teratozoospermia, and oligo-astheno-teratozoospermia. The pathological groups exhibited significant reductions in sperm count, motility, and morphology. Additionally, lipid peroxidation, as shown by increased MDA levels, was significantly elevated in all pathological groups. The activities of CAT, SOD, and GPx were significantly diminished in these groups, with the most substantial declines noted in the oligo-astheno-teratozoospermia group.

Conclusion: Oxidative stress, indicated by elevated MDA levels, was correlated with poor sperm quality. The decreased activity of antioxidant enzymes in pathological semen implies that a weakened antioxidant defense contributes to sperm dysfunction. These findings suggest that antioxidant interventions could improve sperm quality in men experiencing infertility, though additional research is required.

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