Clinical and Experimental Reproductive Medicine-CERM最新文献

{"title":"Impact of orally and vaginally administered estradiol on live birth and neonatal outcomes in frozen-thawed embryo transfer cycles: A retrospective cohort study.","authors":"Yuan Liu, Jian Sun, Yanmei Hu, Yu Wu, Yixia Yang","doi":"10.5653/cerm.2025.07969","DOIUrl":"https://doi.org/10.5653/cerm.2025.07969","url":null,"abstract":"<p><strong>Objective: </strong>Previous studies have reported a higher rate of small for gestational age (SGA) newborns from fresh embryo transfers compared with frozen-thawed embryo transfers (FET), potentially due to the supraphysiologic serum estradiol levels observed during controlled ovarian stimulation. This study aimed to evaluate whether different regimens of exogenous estradiol administration influence live birth rates and neonatal outcomes in hormone replacement therapy (HRT)-FET cycles.</p><p><strong>Methods: </strong>We conducted a retrospective analysis of patients undergoing their first FET with HRT-based endometrial preparation between January 2015 and December 2018 at our center, comparing those who received estradiol both orally and vaginally (OVE group) with those who received estradiol orally only (OE group).</p><p><strong>Results: </strong>Patients in the OVE group achieved higher serum estradiol levels and underwent longer durations of estradiol treatment. No significant differences were found in live birth rate (adjusted odds ratio [OR], 1.327; 95% confidence interval [CI], 0.982 to 1.794; p=0.066) or clinical pregnancy rate (adjusted OR, 1.278; 95% CI, 0.937 to 1.743; p=0.121). The estradiol regimen did not affect singleton birth weight (β=-30.962; standard error=68.723; p=0.653), the odds of large for gestational age (adjusted OR, 1.165; 95% CI, 0.545 to 2.490; p=0.694), the odds of SGA (adjusted OR, 0.569; 95% CI, 0.096 to 3.369; p=0.535), or preterm delivery (adjusted OR, 0.969; 95% CI, 0.292 to 3.214; p=0.959).</p><p><strong>Conclusion: </strong>Combined oral and In subsequent cycles, patients received estrogen administration did not alter live birth rates or singleton neonatal outcomes, but was associated with higher serum estradiol levels and potential maternal risks.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145901187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nrf2-ARE signaling pathway as a key regulator of oxidative stress and antioxidant responses in granulosa cells in polycystic ovary syndrome.","authors":"Mona Mahmoodi-Mahallati, Elahe Seyed Hosseini, Hossein Nikzad, Tayyebeh Zamani-Badi, Lida Hosseini, Hamed Haddad Kashani","doi":"10.5653/cerm.2025.07927","DOIUrl":"https://doi.org/10.5653/cerm.2025.07927","url":null,"abstract":"<p><strong>Objective: </strong>Polycystic ovary syndrome (PCOS) is a metabolic and endocrine disorder that frequently results in infertility due to ovulatory dysfunction. Granulosa cells (GCs), which are essential for oocyte maturation, are highly vulnerable to oxidative stress (OS)-induced damage. The nuclear factor erythroid-2-related factor 2 (NRF2)-antioxidant responsive element (ARE) pathway, a major antioxidant mechanism, may help protect GCs from OS. This study investigated NRF2-ARE pathway activity in GCs of PCOS patients compared to healthy fertile controls.</p><p><strong>Methods: </strong>In total, 46 follicular fluid and GC samples were collected from 28 patients diagnosed with PCOS and 18 fertile women aged 20-40 years. After RNA extraction, expression levels of antioxidant genes- heme oxygenase 1 (HO1), peroxiredoxin 1 (PRDX1), superoxide dismutase 1 (SOD1), thioredoxin (TXN), NRF2, and kelch like ECH associated protein 1 (KEAP1)-were measured using quantitative reverse transcription polymerase chain reaction. In addition, total antioxidant capacity (TAC) and nitric oxide (NO) levels were analyzed in follicular fluid, with statistical comparisons performed using the t-test.</p><p><strong>Results: </strong>In GCs of PCOS patients, HO1 expression was downregulated, while PRDX1, SOD1, NRF2, TXN, and KEAP1 were upregulated. NO activity was elevated, and TAC levels were significantly decreased in the follicular fluid of PCOS patients.</p><p><strong>Conclusion: </strong>These findings demonstrate a significant association between antioxidant gene expression in GCs and PCOS. The results suggest that OS plays a critical role in the development and progression of PCOS.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145901221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Free centrifuge sorting for sperm separation improves intracytoplasmic sperm injection outcomes: A systematic review and meta-analysis.","authors":"Fatemeh Dehghanpour, Mohammad Ali Khalili, Amin Salehi-Abargouei, Bryan J Woodward, Leila Motamedzadeh","doi":"10.5653/cerm.2025.08116","DOIUrl":"https://doi.org/10.5653/cerm.2025.08116","url":null,"abstract":"<p><p>In assisted reproductive technology, spermatozoa must be separated from seminal fluid to achieve optimal fertilization capacity. Conventional separation techniques frequently result in elevated reactive oxygen species production and iatrogenic injury due to repeated cell centrifugation. The aim of this systematic review and meta-analysis was to evaluate the effects of free centrifuge sorting (FCS) techniques on intracytoplasmic sperm injection (ICSI) outcomes. A comprehensive literature search was conducted using PubMed, Scopus, Web of Science, and Cochrane databases. The meta-analysis adhered to the Preferred Reporting Items for Systematic reviews and Meta-Analyses Protocols (PRISMA-P) guidelines. All eligible studies were selected using the population, intervention, comparison/comparator, outcomes, and study design (PICOS) methodology. The primary outcomes assessed were fertilization rate (FR), the high-quality embryo rate, implantation rate (IR), and clinical pregnancy rate (CPR). The study is registered in PROSPERO under registration number CRD42023415532. After screening 306 records for eligibility, three studies were ultimately included in the analysis. Our results demonstrate that following ICSI, a very brief period of abstinence significantly increased IR and CPR. However, no significant differences were observed for FR. The FCS technique yielded spermatozoa of superior biological quality following removal of seminal samples, and this purified sperm population improved reproductive outcomes in ICSI programs.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145764055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence-powered oocyte evaluation: Correlating cytoplasmic features with blastocyst development.","authors":"Hyung Min Kim, Jin Heo, Hyoeun Kang, Kyungmi Juhn, Eunhye Jung, Hyejeong Park, Juhee Jin, Jinho Lim, Hye Jun Lee, Jeong Yoon","doi":"10.5653/cerm.2025.08396","DOIUrl":"https://doi.org/10.5653/cerm.2025.08396","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to establish a quantitative and interpretable method for assessing oocyte quality by analyzing cytoplasmic morphology and intensity features using artificial intelligence.</p><p><strong>Methods: </strong>A total of 695 oocyte images were collected from hormonally stimulated young and aged mice. The cytoplasmic region was manually annotated to exclude polar bodies, and radiomics analysis was performed to extract morphological and intensity-based features.</p><p><strong>Results: </strong>Clustering with a Gaussian mixture model identified three distinct oocyte subtypes with unique cytoplasmic characteristics. Cluster 2, with the most spherical and compact oocytes, demonstrated the highest blastocyst formation rate (42.9%), followed by clusters 3 (35.3%) and 1 (20.4%). Cluster 2 oocytes also showed the highest mean intensity and lowest variability, suggesting uniform cytoplasmic structure. Notably, some aged oocytes in cluster 2 exhibited developmental potential comparable to that of young mice, indicating that cytoplasmic quality may be a more informative predictor than age alone.</p><p><strong>Conclusion: </strong>These findings underscore the value of cytoplasmic features as objective indicators of developmental competence. This artificial intelligence-driven approach may improve embryo selection by providing a standardized, non-invasive method for evaluating oocytes, ultimately contributing to enhanced clinical outcomes in assisted reproductive technologies.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145716222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of mammalian target of rapamycin inhibitors in mitigating cyclophosphamide-induced ovarian damage in a murine model.","authors":"Seul Ki Kim, Byung Chul Jee","doi":"10.5653/cerm.2025.07892","DOIUrl":"10.5653/cerm.2025.07892","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to determine whether pre-treatment with everolimus or rapamycin could prevent ovarian follicle damage induced by cyclophosphamide (Cp).</p><p><strong>Methods: </strong>A total of 120 female BDF-1 mice were randomly assigned into four groups receiving specific treatments. The control group received normal saline on days 1, 3, 5, and 13. The Cp group received saline on days 1, 3, and 5, followed by Cp administration on day 13. The everolimus+Cp group was given everolimus on days 1, 3, and 5, then Cp on day 13. Similarly, the rapamycin+Cp group received rapamycin on days 1, 3, and 5, followed by Cp on day 13. On day 20, all mice were euthanized, ovaries were harvested for histological analysis, and protein expression levels of B-cell lymphoma-extra large (BCL-xL), caspase 3, and mammalian target of rapamycin were evaluated by Western blot analysis.</p><p><strong>Results: </strong>The number of primordial follicles was lower in the Cp group than in the control group. The everolimus group and the rapamycin group also showed reduced primordial follicle counts. No significant differences were observed in the proportions of G1 primordial or G1 secondary follicles among the groups. Compared to the control group, the Cp group and the everolimus group showed lower proportions of G1 primary follicles. However, the rapamycin group had a G1 primary follicle proportion similar to that of the control group. The Cp, everolimus, and rapamycin groups exhibited lower proportions of G1 antral follicles than the control group.</p><p><strong>Conclusion: </strong>Pre-treatment with rapamycin preserved the proportion of G1 primary follicles; however, neither everolimus nor rapamycin preserved the primordial follicle counts.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"359-366"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12682407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of adiposity indices with insulin resistance among women with polycystic ovary syndrome.","authors":"Md Shahed Morshed, Hurjahan Banu, Md Shamim Hossan, Hafsa Mahrukh, Muhammad Abul Hasanat","doi":"10.5653/cerm.2024.07388","DOIUrl":"10.5653/cerm.2024.07388","url":null,"abstract":"<p><strong>Objective: </strong>We compared various adiposity indices (AIs)-body mass index (BMI), waist circumference (WC), visceral adiposity index (VAI), lipid accumulation product, and the triglyceride-glucose (TyG), TyG-BMI, and TyG-WC indices-regarding their associations with insulin resistance (IR) among women with polycystic ovary syndrome (PCOS).</p><p><strong>Methods: </strong>This cross-sectional investigation included 632 reproductive-aged women with PCOS, aligning with the 2018 International Evidence-based Guideline. Clinical characteristics, including height, weight, and WC, were recorded. Fasting blood samples were analyzed to measure insulin, lipid, and glucose levels. AIs were then calculated using these measurements along with various formulas. The homeostatic model assessment for IR was utilized, with a value of 2.6 or higher indicating IR. An area under the curve (AUC) above 0.8 was considered clinically useful.</p><p><strong>Results: </strong>Approximately 69.1% of participants exhibited IR. All AIs were significant predictors of IR, regardless of androgen status. VAI and the TyG index were identified as poor markers of IR among women with PCOS (AUC, 0.6 to 0.69), while the remaining indices were considered fair markers (AUC, 0.7 to 0.79). VAI underperformed all other AIs except the TyG index, which was notably inferior to the TyG-BMI and TyG-WC indices. Nevertheless, the AUCs of TyG-BMI and TyG-WC were similar to those of BMI and WC.</p><p><strong>Conclusion: </strong>Although AIs exhibit androgen-independent relationships with IR, their usefulness as indicators of IR in PCOS is not adequately supported by our results (AUC <0.8). Furthermore, combining the TyG index with BMI and WC does not significantly improve the diagnostic utility of IR.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"52 4","pages":"342-347"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12682414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145702313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between vitamin D levels and erectile dysfunction: A mini-review.","authors":"Aamir Javed, Sanaz A Movassagh, Shilpa Doultani, Tulsi Kumari, Rishish Sharma, Md Kashif Zeya, Syed Waseem Andrabi, Kamal Saba","doi":"10.5653/cerm.2024.07402","DOIUrl":"10.5653/cerm.2024.07402","url":null,"abstract":"<p><p>The importance of maintaining adequate levels of vitamin D for optimal male sexual health is highlighted by recent evidence suggesting a link between vitamin D insufficiency and erectile dysfunction (ED). This review examines current research that indicates an association between decreased serum concentrations of 25-hydroxyvitamin D (25(OH)D), specifically levels below 20 ng/mL, and a higher prevalence of ED, including severe cases. Studies have shown a significant correlation between a decrease of 10 ng/mL in 25(OH)D levels and a 12% increase in the prevalence of ED. The active form of vitamin D, calcitriol, facilitates the synthesis of nitric oxide, a potent vasodilator crucial for penile erection. Additionally, vitamin D supplementation has been shown to improve erectile function by enhancing endothelial vasodilation and arterial blood flow. It is essential to maintain serum 25(OH)D levels within the recommended range of 20 to 50 ng/mL, given the connection between vascular disorders and ED. A comprehensive approach, including dietary changes, consistent physical activity, and lifestyle modifications, is necessary to prevent ED. While vitamin D deficiency may contribute to ED, it is crucial to recognize that ED is multifactorial and should be addressed by considering all underlying causes. Individuals consistently experiencing symptoms of ED are advised to consult healthcare professionals for appropriate therapeutic interventions. This review emphasizes the importance of considering serum vitamin D levels when assessing male sexual health and calls for further research to clarify the role of vitamin D in the etiology and treatment of ED.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"334-341"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12682412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation of Paeonia lactiflora root extract can induce atrophy of endometriotic lesions and accelerate embryo implantation following in vitro fertilization in endometriosis: An experimental study.","authors":"Amir Abdolmaleki, Hadis Amirsayyafi, Saeed Khazaiel, Cyrus Jalili, Kamran Mansourhi, Mitra Bakhtiari","doi":"10.5653/cerm.2024.07374","DOIUrl":"10.5653/cerm.2024.07374","url":null,"abstract":"<p><strong>Objective: </strong>Endometriosis (Endo) involves inflammation and angiogenesis within lesions, potentially causing embryo implantation failure. Paeonia lactiflora (PL) root exhibits anti-angiogenic and anti-inflammatory properties. This experiment investigated the therapeutic effects of PL on endometriotic lesion atrophy and embryo implantation following in vitro fertilization (IVF).</p><p><strong>Methods: </strong>Female mice (n=32) were allocated into treatment and sham groups. Endo was induced through xenograft transplantation of rat endometrium to anterior abdominal walls of recipient (Endo) mice. PL root was extracted, phytochemically characterized, and orally administered (1.06 mg PL/20 g mouse) for 17 consecutive days. Through IVF, cultured mouse embryos were implanted into Endo mouse uteri. Ten days post-IVF, samples were collected, including intra-abdominal fluid for measurement of vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNF-α) using enzyme-linked immunosorbent assay. Embryo-containing uteri underwent trypan blue staining, while uterus fragments were stained with hematoxylin and eosin and analyzed for leukemia inhibitory factor (LIF) gene expression using quantitative polymerase chain reaction. The number of embryo implantation sites and diameter of endometriotic lesions were recorded. Data were analyzed using SPSS ver. 19, with p-values <0.05 considered to indicate statistical significance.</p><p><strong>Results: </strong>Following Endo induction, TNF-α and VEGF levels and lesion diameter increased (p<0.05). LIF gene expression and embryo implantation rate decreased (p<0.05). After PL extract administration to Endo mice, TNF-α levels, VEGF levels, and lesion diameter decreased (p<0.05), while LIF gene expression and implantation rate increased (p<0.05).</p><p><strong>Conclusion: </strong>PL extract (1.06 mg/20 g mouse) decreases TNF-α and VEGF levels, suppressing inflammation and angiogenesis and causing endometriotic lesion atrophy. Furthermore, PL increases uterine LIF gene expression, promoting successful implantation post-IVF in Endo mice.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"348-358"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12682409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress and its correlation with sperm parameters in different semen quality groups.","authors":"Maroua Ben Rhouma, Hatem Bahri, Mustapha Ben Khalifa, Mohsen Sakly, Khémais Ben Rhouma, Moncef Benkhalifa, Olfa Tebourbi","doi":"10.5653/cerm.2024.07668","DOIUrl":"10.5653/cerm.2024.07668","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated oxidative stress and its impact on sperm quality in men with infertility, focusing on lipid peroxidation and the activity of antioxidant enzymes-catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)-in seminal fluid.</p><p><strong>Methods: </strong>This study was conducted from January 2021 to January 2023 and involved 163 male patients who had been experiencing infertility for over a year. The participants were categorized according to semen quality. Semen samples were analyzed for sperm concentration, motility, and morphology following the World Health Organization guidelines. Oxidative stress was evaluated by measuring levels of malondialdehyde (MDA), an indicator of lipid peroxidation, as well as the activity of CAT, SOD, and GPx. Ethical approval and informed consent were obtained from all participants.</p><p><strong>Results: </strong>Semen quality and oxidative stress were evaluated in cases of male infertility, with patients categorized into five groups: normozoospermia, oligozoospermia, asthenozoospermia, teratozoospermia, and oligo-astheno-teratozoospermia. The pathological groups exhibited significant reductions in sperm count, motility, and morphology. Additionally, lipid peroxidation, as shown by increased MDA levels, was significantly elevated in all pathological groups. The activities of CAT, SOD, and GPx were significantly diminished in these groups, with the most substantial declines noted in the oligo-astheno-teratozoospermia group.</p><p><strong>Conclusion: </strong>Oxidative stress, indicated by elevated MDA levels, was correlated with poor sperm quality. The decreased activity of antioxidant enzymes in pathological semen implies that a weakened antioxidant defense contributes to sperm dysfunction. These findings suggest that antioxidant interventions could improve sperm quality in men experiencing infertility, though additional research is required.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"386-393"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12682413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of platelet-rich plasma as a new additive on human embryo development and clinical outcomes.","authors":"Chang-Seop Hyun, Mi-Ji Park, Eun-Jeong Jang, An-Na Kim, Seong-Ho Yang, Yong-Soo Hur, Yeong-Je Gang, Jin-Ho Lim","doi":"10.5653/cerm.2025.08228","DOIUrl":"10.5653/cerm.2025.08228","url":null,"abstract":"<p><strong>Objective: </strong>In this study, we investigated whether adding autologous platelet-rich plasma (PRP) to the culture medium affects embryo development and clinical outcomes in patients with recurrent implantation failure (RIF).</p><p><strong>Methods: </strong>This study included 201 patients with previous RIF. Of these, 77 opted to receive the treatment and were assigned to the PRP group, and 124 declined and were assigned to the control group. In the PRP group, normally fertilized embryos were cultured in medium supplemented with 5% PRP, whereas embryos in the control group were cultured without PRP. Embryo transfer was performed on day 3 after evaluation of embryo quality. A comparative analysis was then conducted between the two groups, focusing on embryo quality and clinical outcomes.</p><p><strong>Results: </strong>Although no significant differences were observed in fertilization or cleavage rates, the PRP group had a significantly higher proportion of good-quality embryos with at least six cells on day 3 than the control group. The clinical pregnancy and implantation rates in the PRP group were also significantly higher than those in the control group. Furthermore, the ongoing pregnancy rate was notably higher in the PRP group, and successful live births were achieved. Miscarriage rates were similar between groups.</p><p><strong>Conclusion: </strong>Incorporating PRP as an additive into the culture medium improved embryo quality and increased implantation, clinical pregnancy, and ongoing pregnancy rates.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"367-375"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12682411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145597414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}