Maternal exposure to phenanthrene induces testicular apoptosis and Sertoli cell dysfunction in F1 adult male mice: a histological and molecular study.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Azar Afshar, Hamid Nazarian, Fatemeh Fadaefathabadi, Fakhroddin Aghajanpour, Reza Soltani, Mohammad-Amin Abdollahifar, Gholamreza Hassanzadeh, Mohsen Nourozian
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Abstract

Objective: Phenanthrene, a polycyclic aromatic hydrocarbon, is found in abundance in environmental pollutants, food, and drinking water. This substance can accumulate in body tissues and exert harmful effects. Moreover, phenanthrene can cross the placental barrier, potentially impacting fetal development. We aimed to explore the impacts of maternal exposure to phenanthrene on testicular tissue and Sertoli cell function in F1 mice.

Methods: Female rats with vaginal plugs were randomly assigned to one of three groups: control, sham, or phenanthrene. The control group received no intervention during pregnancy. In the sham and phenanthrene groups, corn oil and a phenanthrene solution, respectively, were administered via gavage once every 2 days. Offspring were separated by sex 21 days after birth. At 56 days postnatal, male F1 offspring were euthanized, and their testes were harvested for histological and molecular analyses.

Results: Phenanthrene exposure was associated with a lower testicular weight and volume, a smaller diameter of the seminiferous tubules, and a relative thinning of the germinal epithelium. These changes were associated with increased cellular apoptosis, as shown by the upregulation of caspase 3 expression. Additionally, we observed an increase in vacuolization and residual bodies within the tissue. Conversely, the number of Sertoli cells and expression levels of Sox9, as well as the Ocln and Itgb1 genes, were found to be lowered.

Conclusion: Maternal exposure to phenanthrene impacts both germ cells and Sertoli cells, disrupting their function and leading to fertility disorders in male F1 offspring mice.

母体暴露于菲会诱发 F1 成年雄性小鼠睾丸凋亡和 Sertoli 细胞功能障碍:一项组织学和分子研究。
目的:菲是一种多环芳烃,大量存在于环境污染物、食物和饮用水中。这种物质可在人体组织中蓄积并产生有害影响。此外,菲还能穿过胎盘屏障,对胎儿发育造成潜在影响。我们旨在探讨母体接触菲对 F1 小鼠睾丸组织和 Sertoli 细胞功能的影响:方法:将带有阴道塞的雌性大鼠随机分配到三组中的一组:对照组、假组或菲组。对照组在怀孕期间不接受任何干预。在假组和菲组中,每两天分别灌胃一次玉米油和菲溶液。后代在出生后 21 天按性别分开。在出生后 56 天,雄性 F1 后代被安乐死,并收获其睾丸进行组织学和分子分析:结果:接触菲后,睾丸重量和体积降低,曲细精管直径变小,生精上皮相对变薄。这些变化与细胞凋亡的增加有关,如 caspase 3 表达的上调。此外,我们还观察到组织内空泡化和残留体的增加。相反,我们发现 Sertoli 细胞的数量和 Sox9 以及 Ocln 和 Itgb1 基因的表达水平降低:结论:母体接触菲会影响生殖细胞和Sertoli细胞,破坏它们的功能,导致雄性F1后代小鼠出现生育障碍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
3.30
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