Clinical and Experimental Reproductive Medicine-CERM最新文献

{"title":"State-of-the-art in high throughput organ-on-chip for biotechnology and pharmaceuticals.","authors":"Suk-Heung Song, Sehoon Jeong","doi":"10.5653/cerm.2024.06954","DOIUrl":"10.5653/cerm.2024.06954","url":null,"abstract":"<p><p>Modern drug discovery is driven by high demand in the pharmaceutical industry to test growing libraries of compounds against potential targets. High-throughput screening (HTS) is characterized by fully automated experimentation that leverages robotic liquid handling systems, analytical techniques, and advanced computing and statistics, including the recent integration of artificial intelligence. To align with this trend, it is crucial to develop and implement new HTS platforms that offer improved predictivity and physiological relevance. In recent years, microphysiological systems, commonly known as organ-on-chip (OoC) systems, have progressed from a theoretical concept to a powerful alternative to conventional in vitro and animal models. High-throughput OoC (HT-OoC) systems could represent the disruptive technology sought by pharmaceutical companies to address their enormous research and development (R&D) expenses. In this study, we provide a brief overview of commercial products utilizing modern HT-OoC systems in drug discovery and development. Additionally, we discuss recent trends in R&D aimed at industrialization.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"114-124"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation of sperm based on rheotaxis mechanism using a microfluidic device.","authors":"Hossein Torkashvand, Shamim Pilehvari, Ronak Shabani, Rana Mehdizade, Leila Torkashvand, Mahdi Moghimi, Roya Derakhshan, Mehdi Mehdizadeh","doi":"10.5653/cerm.2024.07304","DOIUrl":"https://doi.org/10.5653/cerm.2024.07304","url":null,"abstract":"<p><strong>Objective: </strong>In the application of assisted reproductive technologies (ART), selection of the optimal sperm presents a challenge. This study introduces an innovative microfluidic device that utilizes rheotaxis to efficiently sort sperm, offering superior selection of high-quality sperm compared to conventional methods.</p><p><strong>Methods: </strong>We analyzed 30 normal samples from couples undergoing intracytoplasmic sperm injection cycles at the Infertility Center of Fatemieh Hospital in Hamadan, Iran. Each sample was divided into three groups: the initial sample, representing the control group; direct swim-up sperm selection; and sperm selection using rheotaxis. A syringe pump connected to the microfluidic device generated optimal flow conditions. Spermatozoa were evaluated regarding concentration, motility, morphology, mitochondrial membrane potential (MMP), and sperm DNA fragmentation index (DFI). Statistical significance was determined using one-way analysis of variance and the Student t-test.</p><p><strong>Results: </strong>The concentration (7.46±2.84 million cells/mL vs. 56.67±18.27 million cells/mL, p<0.0001) and DFI (2.93±2.70 vs. 21.13±5.27, p<0.0001) were significantly lower in the sperm selected using the rheotaxis microfluidic device than in the control sperm. Progressive motility (98.10%±2.41% vs. 44.13%±7.06%, p<0.0001), normal morphology (8.36%±1.47% vs. 5.20%±1.15%, p<0.0001), and MMP (99.63%±0.71% vs. 81.13%±9.19%, p<0.0001) were significantly higher with the device than in the control group.</p><p><strong>Conclusion: </strong>The use of a rheotaxis-based microfluidic device appeared effective in selecting high-quality sperm, demonstrating improvements in motility, morphology, and MMP and a reduction in DFI. This advancement has the potential to improve the outcomes of ART.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress and its correlation with sperm parameters in different semen quality groups.","authors":"Maroua Ben Rhouma, Hatem Bahri, Mustapha Ben Khalifa, Mohsen Sakly, Khémais Ben Rhouma, Moncef Benkhalifa, Olfa Tebourbi","doi":"10.5653/cerm.2024.07668","DOIUrl":"https://doi.org/10.5653/cerm.2024.07668","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated oxidative stress and its impact on sperm quality in men with infertility, focusing on lipid peroxidation and the activity of antioxidant enzymes-catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)-in seminal fluid.</p><p><strong>Methods: </strong>This study was conducted from January 2021 to January 2023 and involved 163 male patients who had been experiencing infertility for over a year. The participants were categorized according to semen quality. Semen samples were analyzed for sperm concentration, motility, and morphology following the World Health Organization guidelines. Oxidative stress was evaluated by measuring levels of malondialdehyde (MDA), an indicator of lipid peroxidation, as well as the activity of CAT, SOD, and GPx. Ethical approval and informed consent were obtained from all participants.</p><p><strong>Results: </strong>Semen quality and oxidative stress were evaluated in cases of male infertility, with patients categorized into five groups: normozoospermia, oligozoospermia, asthenozoospermia, teratozoospermia, and oligo-astheno-teratozoospermia. The pathological groups exhibited significant reductions in sperm count, motility, and morphology. Additionally, lipid peroxidation, as shown by increased MDA levels, was significantly elevated in all pathological groups. The activities of CAT, SOD, and GPx were significantly diminished in these groups, with the most substantial declines noted in the oligo-astheno-teratozoospermia group.</p><p><strong>Conclusion: </strong>Oxidative stress, indicated by elevated MDA levels, was correlated with poor sperm quality. The decreased activity of antioxidant enzymes in pathological semen implies that a weakened antioxidant defense contributes to sperm dysfunction. These findings suggest that antioxidant interventions could improve sperm quality in men experiencing infertility, though additional research is required.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into pentoxifylline and α-lipoic acid: Co-administration with clomiphene citrate for ovulation induction in anovulatory women with polycystic ovary syndrome.","authors":"Ahmed A Morsy, Nagwa A Sabri, Abdelrehim M Mourad, Eman M Mojahed, Sarah F Fahmy","doi":"10.5653/cerm.2024.07346","DOIUrl":"https://doi.org/10.5653/cerm.2024.07346","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effects of pentoxifylline (PTX) and α-lipoic acid (ALA) on ovulation and pregnancy rates in women with clomiphene citrate (CC) resistant polycystic ovary syndrome (PCOS).</p><p><strong>Methods: </strong>A prospective, randomized, controlled, open-label study was conducted on women with CC-resistant PCOS. In total, 120 PCOS patients were randomly assigned to four groups of 30 patients each, as follows: group 1 was the control group; group 2 received 400 mg of PTX twice daily; group 3 received 600 mg of ALA twice daily; and group 4 received a combination of PTX and ALA, following the same regimen as the previous groups. All groups were administered 150 mg of CC, the standard therapy for ovulation induction (ClinicalTrials.gov: NCT05231980).</p><p><strong>Results: </strong>The cumulative ovulation rate was highest in the combined PTX-ALA group at 77% (23 cases), followed by the PTX group at 70% (n=21), the ALA group at 40% (n=12), and the control group at 30% (n=9) (p=0.0003). The cumulative pregnancy rates were 40% (n=12), 37% (n=11), 10% (n=3), and 3% (n=1) for the PTX-ALA, PTX, ALA, and control groups, respectively (p=0.0005). Endometrial thickness (ET) was significantly greater in the PTX group than in the control group.</p><p><strong>Conclusion: </strong>Co-administration of PTX with CC significantly improved the ovulation rate, pregnancy rate, ET, and ovarian response to stimulation in patients with anovulatory PCOS. This combination may provide an effective, affordable, and safe treatment protocol for women with CC-resistant PCOS.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WT1 pathogenic variant in azoospermic infertile men with an isolated undescended testis.","authors":"Neda Sharifi, Parnaz Borjian Boroujeni, Kaveh Haratian, Marjan Sabbaghian, Anahita Mohseni Meybodi","doi":"10.5653/cerm.2024.07584","DOIUrl":"https://doi.org/10.5653/cerm.2024.07584","url":null,"abstract":"<p><strong>Objective: </strong>An undescended testis (UDT) is a testicle that has not moved into the scrotum before birth. UDTs are linked to reduced fertility, primarily due to compromised semen quality and potential dysfunction in Sertoli and Leydig cells. Additionally, the Wilms tumor 1 (WT1) gene is crucial for spermatogenesis, as it regulates the polarity of Sertoli cells and the steroidogenesis in Leydig cells. Our study aimed to identify novel UDT-causing WT1 variants within a cohort of 60 unrelated men with infertile hypergonadotropic hypogonadism.</p><p><strong>Methods: </strong>In this case-control study, the coding regions and the intronic boundaries of the second and ninth exon of WT1 were sequenced using Sanger sequencing. DNA from 60 fertile men served as the control group. In silico analysis of the variants was also conducted.</p><p><strong>Results: </strong>The study identified multiple intronic and exonic variations in both the patient and control groups. Notably, a haplotype consisting of two heterozygous C>T variations in the intronic region of the splice donor site of exon 9 was observed in 11 patients but was absent in the control group. Of these variations, only one has been previously reported in Single Nucleotide Polymorphism Database (dbSNP) as rs587776576 (NC_000011.10: g.32391967C>T; NM_000378.4:c.1372+14G>A).</p><p><strong>Conclusion: </strong>The rs587776576 mutation is pathogenic. It exhibited a significant association (p=0.022), indicating its association with infertility and UDT in the Iranian population. This research could broaden the spectrum of WT1 variations and underscore the importance of these variants in the genetic etiology of UDT and infertility. These findings provide a foundation for clinical diagnosis and genetic counseling.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between vitamin D levels and erectile dysfunction: A mini-review.","authors":"Aamir Javed, Sanaz A Movassagh, Shilpa Doultani, Tulsi Kumari, Rishish Sharma, Md Kashif Zeya, Syed Waseem Andrabi, Kamal Saba","doi":"10.5653/cerm.2024.07402","DOIUrl":"https://doi.org/10.5653/cerm.2024.07402","url":null,"abstract":"<p><p>The importance of maintaining adequate levels of vitamin D for optimal male sexual health is highlighted by recent evidence suggesting a link between vitamin D insufficiency and erectile dysfunction (ED). This review examines current research that indicates an association between decreased serum concentrations of 25-hydroxyvitamin D (25(OH)D), specifically levels below 20 ng/mL, and a higher prevalence of ED, including severe cases. Studies have shown a significant correlation between a decrease of 10 ng/mL in 25(OH)D levels and a 12% increase in the prevalence of ED. The active form of vitamin D, calcitriol, facilitates the synthesis of nitric oxide, a potent vasodilator crucial for penile erection. Additionally, vitamin D supplementation has been shown to improve erectile function by enhancing endothelial vasodilation and arterial blood flow. It is essential to maintain serum 25(OH)D levels within the recommended range of 20 to 50 ng/mL, given the connection between vascular disorders and ED. A comprehensive approach, including dietary changes, consistent physical activity, and lifestyle modifications, is necessary to prevent ED. While vitamin D deficiency may contribute to ED, it is crucial to recognize that ED is multifactorial and should be addressed by considering all underlying causes. Individuals consistently experiencing symptoms of ED are advised to consult healthcare professionals for appropriate therapeutic interventions. This review emphasizes the importance of considering serum vitamin D levels when assessing male sexual health and calls for further research to clarify the role of vitamin D in the etiology and treatment of ED.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term use of dutasteride to treat androgenic alopecia in young men may lead to persistent abnormalities in semen parameters.","authors":"Young Jae Kim, Seoung Ryeol Lee, Young Dong Yu","doi":"10.5653/cerm.2024.07675","DOIUrl":"https://doi.org/10.5653/cerm.2024.07675","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the long-term effects of dutasteride on male fertility and determine the cutoff treatment duration that causes significant and persistent decreases in semen parameters.</p><p><strong>Methods: </strong>This was a single-center, randomized, controlled study that evaluated 200 men (ages 28 to 39 years). Forty men were allocated to each study group, divided according to the duration of dutasteride treatment, as follows: <6 months (group 1), 6-12 months (group 2), 13-18 months (group 3), 19-24 months (group 4), and >24 months (group 5). All subjects received dutasteride 0.5 mg/day for management of androgenetic alopecia then discontinued dutasteride for 6 months.</p><p><strong>Results: </strong>The baseline mean testosterone level in the study subjects was 4.8 ng/mL. No significant differences were found between study groups in sperm concentration, normal morphology, and vitality. Semen volume and sperm total/progressive motility were significantly reduced as the duration of dutasteride treatment increased. All study groups showed an increase in total sperm motility and semen volume after discontinuation of dutasteride. Compared with group 1, groups 2-5 showed significant decreases in semen volume and sperm total motility, with the odds ratios becoming smaller as the duration of dutasteride treatment increased. Receiver operating characteristic analysis showed the cutoff values for persistent impairment of semen volume and total sperm motility to be 17.8 and 20.3 months, respectively.</p><p><strong>Conclusion: </strong>Long-term use of dutasteride may lead to male infertility by persistently impairing semen volume and sperm motility.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between thyroid-stimulating hormone levels and the severity of vitamin D deficiency by age group.","authors":"Mansi Modi, Pinky Garg","doi":"10.5653/cerm.2023.06779","DOIUrl":"10.5653/cerm.2023.06779","url":null,"abstract":"<p><strong>Objective: </strong>Researchers have long been captivated by the complex molecular interactions between vitamin D and the thyroid gland. Hypothyroidism affects 2% to 4% of women of reproductive age and can impact fertility through anovulatory cycles, luteal phase defects, hyperprolactinemia, and sex hormone imbalances. This study investigated the relationship between thyroid disease and the severity of vitamin D deficiency across different age groups.</p><p><strong>Methods: </strong>A retrospective study was conducted of 286 patient samples from individuals aged 18 to 60 years who were processed in the clinical biochemistry laboratory of our hospital. Samples were tested for thyroid-stimulating hormone (TSH) and vitamin D (specifically, vitamin D3) levels. The study samples were categorized into four clinically relevant groups based on TSH levels and into three groups based on serum 25-hydroxyvitamin D (25(OH)D) levels.</p><p><strong>Results: </strong>Most of the samples were from female patients (n=269), and the most common age group was 18 to 35 years (n=191, 66.78%). Subclinical hypothyroidism was identified in 120 patients, while vitamin D deficiency was present in 237 (82.87%) participants. A significant association was observed between vitamin D deficiency and the presence of thyroid disorders. Additionally, a significant negative correlation was found between TSH and vitamin D levels. Polycystic ovary syndrome was noted in 103 female patients (36.01%).</p><p><strong>Conclusion: </strong>TSH and 25(OH)D levels should be screened in all women of reproductive age, not just those in high-risk groups, as subclinical and occult hypothyroidism may otherwise go undiagnosed. Furthermore, TSH should be considered the primary screening test.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"71-78"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations of dietary inflammatory indices (DII and E-DII) with sperm parameters.","authors":"Sonia Sadeghpour, Fatemeh Maleki Sedgi, Sevana Daneghian, Somayyeh Barania Adabi, Tahereh Behroozi-Lak, Mohammadreza Pashaei, Javad RasouIi, Rohollah Valizadeh, Hojat Ghasemnejad-Berenji","doi":"10.5653/cerm.2024.06982","DOIUrl":"10.5653/cerm.2024.06982","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to explore the ambiguous link between dietary inflammatory indices and sperm parameters. Specifically, it investigated the associations between the dietary inflammatory index (DII) and the energy-adjusted dietary inflammatory index (E-DII) with sperm motility, morphology, and count in men undergoing routine semen analysis.</p><p><strong>Methods: </strong>A cross-sectional study was conducted with 144 men enrolled, where semen samples were collected and evaluated according to the 2010 World Health Organization guidelines. Dietary data were gathered using a 147-item semi-quantitative food frequency questionnaire developed by the researchers. Pearson correlation analysis was employed to assess the relationships of the DII and E-DII with sperm parameters.</p><p><strong>Results: </strong>The mean DII and E-DII scores were 1.23±1.1 and 0.49±0.43, respectively. The mean values for sperm motility, morphology, and count were 43.08%±19.30%, 78.03%±26.99%, and 48.12±44.41 million, respectively. Both motility (r=-0.353) and count (r=-0.348) were found to be inversely and significantly correlated with DII. Similarly, Pearson correlation tests revealed strong and significant inverse correlations of motility (r=-0.389) and count (r=-0.372) with E-DII.</p><p><strong>Conclusion: </strong>The findings suggest that a diet with a higher anti-inflammatory potential may be associated with increased sperm count and motility, but not with changes in morphology. Further research is necessary to confirm these findings, elucidate the underlying mechanisms, and identify dietary modifications that could improve male fertility.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"79-86"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of anxiety, depression, and sexual dysfunction in women undergoing fertility treatment.","authors":"Joon Cheol Park","doi":"10.5653/cerm.2024.07465","DOIUrl":"10.5653/cerm.2024.07465","url":null,"abstract":"<p><strong>Objective: </strong>Infertility and fertility treatment can lead to considerable physical, emotional, and psychological distress. These effects may be influenced by personality, familial bonds, and support systems within a sociocultural context. This preliminary investigation examined anxiety, depression, and sexual dysfunction in Korean women undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI).</p><p><strong>Methods: </strong>Women scheduled for IVF/ICSI and healthy women seeking general health screening (controls) were enrolled. Participants were asked to complete standardized, validated questionnaires-the Hospital Anxiety and Depression Scale (HADS), Depression Anxiety and Stress Scale (DASS), and Female Sexual Function Index (FSFI)-to assess their levels of anxiety, depression, and sexual function.</p><p><strong>Results: </strong>The mean HADS scores for women undergoing IVF/ICSI were 6.35±3.48 (range, 0 to 13) for anxiety and 8.32±3.78 (range, 2 to 15) for depression. However, 12.9% of the women with infertility experienced clinically significant anxiety (HADS-Anxiety score >11), while 32.3% exhibited depression (HADS-Depression score >11). Based on DASS scores, psychological difficulties were more prevalent among women experiencing infertility than among control participants. Total FSFI scores, along with subscale ratings for desire, arousal, lubrication, satisfaction, and pain, were similar between women with infertility and control women. However, the mean score for orgasm was significantly lower in the infertility group (3.16) than among controls. Age, the durations of marriage and infertility, and parity did not significantly influence HADS, DASS, or FSFI scores.</p><p><strong>Conclusion: </strong>Anxiety and depression were more prevalent among women seeking fertility treatment than among healthy controls. Additionally, women with infertility may experience fewer or diminished orgasms.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"52 1","pages":"38-43"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}