Serum miR-329-3p as a potential biomarker for poor ovarian response in an in vitro fertilization.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Jung Hoon Kim, Hye-Ok Kim, Su-Yeon Lee, Eun-A Park, Kyoung Hee Choi, Kiye Kang, Eun Jeong Yu, Mi Kyoung Koong, Kyung-Ah Lee
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Abstract

Objective: Several miRNAs have been identified as differentially expressed in patients with poor ovarian response (POR) compared to those with normal responses. This study aims to assess the potential of serum miR-329-3p as a biomarker for diagnosing POR.

Methods: We conducted a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis to confirm the target genes of miR-329-3p. KGN cells were transfected with both miR-329-3p mimic and inhibitor to assess the differential expression of these target genes. In accordance with the Bologna criteria, we enrolled 16 control patients and 16 patients with POR. We collected patient samples, including serum from day 2 and the human chorionic gonadotropin (hCG) day, as well as granulosa and cumulus cells, to validate the expression of miR-329-3p using quantitative real-time polymerase chain reaction.

Results: KEGG pathway analysis revealed that miR-329-3p targeted adenylyl cyclase 9 (ADCY9) and protein kinase A subunit beta (PRKACB), both of which are involved in ovarian steroidogenesis. In KGN cells treated with a miR-329-3p mimic, ADCY9 and PRKACB expression levels were significantly reduced (p<0.05). Elevated levels of miR-329-3p suppressed aromatase expression and 17β-estradiol production by modulating ADCY9 and PRKACB in KGN cells. These effects were also observed in POR patients. Follicle-stimulating hormone receptor (FSHR) expression was diminished in the granulosa cells of POR patients. On day 2, on hCG day, and in granulosa cells, miR-329-3p exhibited high expression levels in the serum of POR patients.

Conclusion: miR-329-3p exhibited increased expression in granulosa cells and in the sera of POR patients. Consequently, we propose that miR-329-3p may be a potential biomarker for the diagnosis of POR.

血清miR-329-3p作为体外受精卵巢不良反应的潜在生物标志物。
目的:几种mirna在卵巢反应不良(POR)患者中与正常反应患者相比存在差异表达。本研究旨在评估血清miR-329-3p作为诊断POR的生物标志物的潜力。方法:我们通过京都基因与基因组百科全书(KEGG)通路分析来确认miR-329-3p的靶基因。转染了miR-329-3p模拟物和抑制剂的KGN细胞,以评估这些靶基因的差异表达。按照博洛尼亚标准,我们招募了16名对照患者和16名POR患者。我们收集了患者样本,包括第2天的血清和人绒毛膜促性腺激素(hCG)天的血清,以及颗粒和积云细胞,使用定量实时聚合酶链反应验证miR-329-3p的表达。结果:KEGG通路分析显示,miR-329-3p靶向腺苷酸环化酶9 (ADCY9)和蛋白激酶A亚单位β (PRKACB),两者都参与卵巢类固醇生成。在用miR-329-3p模拟物处理的KGN细胞中,ADCY9和PRKACB的表达水平显著降低(p结论:miR-329-3p在颗粒细胞和POR患者血清中的表达增加。因此,我们提出miR-329-3p可能是诊断POR的潜在生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
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