Analytical and Bioanalytical Chemistry最新文献

{"title":"A novel electrochemical sensor based on Co,N co-doped porous carbon nanocage-carbon nanotube composites for real-time electrochemical monitoring of hydrogen peroxide release in cancer cells.","authors":"Zhiqi Liang, Li Cheng, Qichun Zha, Lin Chen, Wenting Yan, Dan Jiang, Zhouling Wu","doi":"10.1007/s00216-026-06840-4","DOIUrl":"https://doi.org/10.1007/s00216-026-06840-4","url":null,"abstract":"<p><p>Hydrogen peroxide (H₂O₂), a key reactive oxygen species (ROS) in vivo, plays an important role in host immune defense against pathogen invasion. Abnormal H₂O₂ levels are associated with various diseases; therefore, accurate and real-time detection of H₂O₂ is of considerable biomedical relevance. In this study, a composite material consisting of Co,N co-doped porous carbon nanocages (Co,N-PC) and carbon nanotubes (CNTs) was fabricated and exhibited superior electrocatalytic performance toward H₂O₂ reduction compared with Co,N-PC without CNTs. The synergistic interaction between CNTs and Co,N-PC enhanced the electrical conductivity and electrochemically active surface area of Co,N-PC@CNTs, thereby improving its electrocatalytic activity and non-enzymatic electrochemical sensing performance toward H₂O₂. Under optimal conditions, the Co,N-PC@CNTs-modified rotating disk electrode (RDE) exhibited a wide linear concentration range of 0.005-9.5 mM, a high sensitivity of 460.245 μA·mM^-1·cm^-2, and a low detection limit of 0.060 μM (S/N = 3). Its overall analytical performance surpassed that of the state-of-the-art ZIF-67-derived carbon sensors reported in the literature. Density functional theory (DFT) calculations demonstrated that the introduction of CNTs in conjunction with Co,N-PC effectively modulated the electronic structure and optimized the d-band center, thereby reducing the Gibbs free energy of the rate-determining step, lowering the reaction energy barrier, and accelerating the reaction kinetics. In addition, the sensor exhibited a measurable electrochemical response to H₂O₂ associated with PMA-stimulated HT29 cells. Therefore, the Co,N-PC@CNTs/RDE sensor provides a promising platform for sensitive H₂O₂ detection, with potential applications in biological, food, and environmental analysis.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: An excitation‑customized ICT‑type turn‑on probe for visualization of cysteine fluctuation in diverse drug‑induced ferroptosis pathways.","authors":"Yi-Jun Gong, Wenxue Shi, Aiying Ma, Qian Wang, Wenjuan Lou, Minglu Zhang","doi":"10.1007/s00216-026-06844-0","DOIUrl":"https://doi.org/10.1007/s00216-026-06844-0","url":null,"abstract":"","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of apparent amylose in rice using microfluidic paper-based analytical devices based on Ce-UiO66 with high oxidase-like activity.","authors":"Yinjun Li, Jing Wang, Pei Zhang, Zhenzhen Xia, Zewei Luo, Jie Yang","doi":"10.1007/s00216-026-06805-7","DOIUrl":"https://doi.org/10.1007/s00216-026-06805-7","url":null,"abstract":"<p><p>Apparent amylose content is a key determinant of rice flavor quality, yet traditional detection methods are often costly and time-consuming. To address this, we developed Ce-UiO66 nanozyme-based microfluidic paper-based analytical devices (μPADs) for the rapid quantification of apparent amylose. The sensing mechanism relies on the formation of a blue-purple apparent amylose-iodine complex via in situ catalytic oxidation, which is quantified through smartphone-based grayscale analysis. Mechanistic studies reveal that the catalytic activity of Ce-UiO66 is driven by O₂^- and H₂O₂ intermediates. The tri-zonal design of the μPADs (droplet, reaction, and detection zones) effectively minimizes in situ reaction interference. Under optimized conditions, the method achieved a linear range of 0-40% within 15 min, showing excellent agreement with flow injection analysis. This Ce-UiO66@μPAD platform demonstrates significant potential as a low-cost, portable tool for rapid monitoring of apparent amylose in rice.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated internal standard volumetric dried blood microsampling for the quantification of phosphatidylethanol 16:0/18:1.","authors":"Martina Galletto, Donald Chace, Manasi Kamat, Timothy J Garrett, Alberto Salomone","doi":"10.1007/s00216-026-06828-0","DOIUrl":"https://doi.org/10.1007/s00216-026-06828-0","url":null,"abstract":"<p><p>Phosphatidylethanol 16:0/18:1 (PEth) is a direct biomarker of alcohol consumption, formed in red blood cell membrane in the presence of ethanol. Concentrations of 20 ng/mL and 200 ng/mL have been proposed as cut-offs to distinguish abstinence or low alcohol consumption from moderate drinking and to indicate alcohol misuse, respectively. Dried blood spots (DBS) are considered a preferred matrix for PEth determination because of its limited stability in liquid whole blood. However, conventional DBS workflows may compromise quantitative accuracy, as the internal standard does not fully compensate for extraction-related variability. In this study, we investigated the incorporation of an isotopically labelled internal standard (d5-PEth) directly into the sample disc of the Capitainer®B10 Vanadate card. A rapid UHPLC-MS/MS method based on selected reaction monitoring (SRM), with a total run time of 5 min, was developed together with an extraction protocol suitable for routine application. The method was validated in terms of calibration performance, sensitivity, accuracy, precision, matrix effect, and extraction recovery. The limit of detection was 4.2 ng/mL, moderate ion suppression was observed, and extraction recovery was 57 ± 18%. The resulting internal quantification dried blood spot (iqDBS) workflow showed satisfactory repeatability across 48 spiked samples extracted over three analytical sessions, with CV% below 15%. The stability of the device was also confirmed for up to 2.5 months after preparation under accelerated aging conditions at elevated temperature. Overall, the proposed iqDBS strategy provides a robust and promising framework for PEth quantification, improving the reliability of alcohol biomarker assessment in clinical and forensic applications.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149029470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid serum-based differentiation of ovarian tumors and assessment of post-treatment disease-free status using portable Raman spectroscopy and machine learning.","authors":"Huan Wang, Jinhong Wang, Xingyue Gong, Yixuan Zhang, Siyuan Zhang, Yimin Han","doi":"10.1007/s00216-026-06823-5","DOIUrl":"https://doi.org/10.1007/s00216-026-06823-5","url":null,"abstract":"<p><p>Rapid, low-cost tools for ovarian tumor discrimination and post-treatment assessment remain limited because conventional serum biomarkers such as CA125 have suboptimal sensitivity and specificity. Serum contains proteins, lipids, carbohydrates, and nucleic acids that generate disease-related spectrochemical fingerprints, which portable Raman spectroscopy can capture with minimal sample preparation. We investigated serum Raman profiling for ovarian tumor discrimination and cross-sectional characterization of post-treatment disease-free status. Serum samples from 280 participants (85 benign, 82 malignant, and 113 independent follow-up patients) were analyzed over 400-1800 cm⁻¹. Candidate regions were re-selected in each training set and represented by local mean intensity or an 11-point local spectral profile (Raw11). Four classifiers were evaluated using fivefold cross-validation, repeated nested fivefold cross-validation, 100 stratified 70:30 holdout repetitions, and label-permutation testing. Raw11 consistently outperformed mean intensity. RBF-SVM showed balanced discrimination and stability (AUC = 0.969, accuracy = 0.940, sensitivity = 0.941, specificity = 0.939), with a repeated holdout AUC of 0.943 ± 0.054 and permutation P = 0.001996. Representative Raman regions were not significantly correlated with continuous CA125, indicating that Raman features were not simple surrogates for CA125. Follow-up samples were strongly distinguishable from active malignancy (RBF-SVM AUC = 0.996) but less separable from benign samples (random forest AUC = 0.840), and frozen benign-malignant models placed them closer to benign spectral space. These findings support portable serum Raman spectroscopy as a rapid, label-free adjunct for ovarian tumor discrimination and cross-sectional assessment of post-treatment disease-free serum phenotypes.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149023615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and certification of novel high-protein food certified reference materials.","authors":"Kelly L LeBlanc, Kenny Nadeau, Lu Yang, Christine Brophy, Indumathi Pihillagawa Gedara, Calvin Palmer, Juris Meija, Zoltán Mester, Patricia Grinberg","doi":"10.1007/s00216-026-06816-4","DOIUrl":"https://doi.org/10.1007/s00216-026-06816-4","url":null,"abstract":"<p><p>In response to the growing market for alternative high-protein foods, the National Research Council of Canada (NRC) has produced a series of five new certified reference materials (CRMs) including insect-based (black soldier fly larvae, cricket flour, and mealworm powder; BFLY-1, KRIK-1, and VORM-1) and plant-based proteins (canola meal and seaweed powder; CAME-1 and GSEA-1), in addition to the newest iteration of the fish protein CRM, DORM-5. Data for the mass fractions of up to 33 trace and essential elements were obtained for each material, along with speciation data for arsenic and selenium. Values for each analyte are presented on the materials' Certificates of Analysis, on a dry mass basis, and include estimates of their expanded uncertainties corresponding to 95% confidence (k = 2). Herein, we describe the methods involved in the characterization campaigns for these materials, including the steps taken to overcome associated analytical challenges. We also describe a new approach to consensus value determination, via bias correction of measured results based on the quality control measurement of several similar-matrix CRMs, followed by the use of the Laplace random effects model for data combination.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149023610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryoprotective strategies for frozen erythrocytes in POCT whole blood reference materials progress and challenges.","authors":"Kaixiang Gao, Haofeng Sun, JiaXin Yu, Qi Zhang, Jianyi Liu, Jie Li, Lixiong Wen, Dewei Song","doi":"10.1007/s00216-026-06812-8","DOIUrl":"https://doi.org/10.1007/s00216-026-06812-8","url":null,"abstract":"<p><p>Point-of-care testing (POCT) is widely applied in emergency and primary healthcare, but persistent detection bias compromises its reliability. The core causes are erythrocyte matrix effects and the global shortage of commutable whole blood matrix reference materials, while erythrocyte hemolysis during cryopreservation further exacerbates measurement errors and metrological traceability failure. This paper reviews key cryoprotective technologies for frozen erythrocytes over the past decade, including optimized glycerol protocols, novel carbohydrate and non-carbohydrate cryoprotective agents (CPAs), and erythrocyte silicification. Optimized glycerol methods control hemolysis below 0.8% but carry risks of residual interference. Carbohydrate CPAs exhibit excellent biocompatibility and potent ice recrystallization inhibition, yet are limited by low transmembrane efficiency. Non-carbohydrate CPAs are easily removable and free of detection interference, but require improvement in human erythrocyte adaptability. Erythrocyte silicification achieves a 96.9% recovery rate, yet faces high costs, scaling difficulties, and unvalidated clinical safety. Current research gaps include the lack of matrix effect verification, ambiguous residual interference mechanisms, and insufficient long-term stability data. Future efforts should develop low-residue, user-friendly, human-erythrocyte-adapted cryoprotective techniques, strengthen commutability validation, and industrialize freeze-dried whole blood reference materials to minimize hemolysis and matrix interference. This will advance the standardization of in vitro diagnostics and accurate POCT quantitation, ultimately enhancing diagnostic accuracy and patient safety in decentralized healthcare.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-guided SERS-active micromotor clusters for on-site detection of microorganisms.","authors":"Limei Liu, Taimin Sun, Yunyu Sun, Xuyang Feng, Enbo Xue, Binghao Wang","doi":"10.1007/s00216-026-06821-7","DOIUrl":"https://doi.org/10.1007/s00216-026-06821-7","url":null,"abstract":"<p><p>Surface-enhanced Raman spectroscopy (SERS) provides ultrasensitive molecular fingerprinting, but conventional static SERS substrates are often limited by inefficient analyte capture and poor spatial controllability. Herein, we report a light-driven micromotor SERS detector based on silver-coated polystyrene microspheres (Ag@PS) for active, localized, and rapid biochemical sensing. The Ag@PS micromotors were fabricated through an electroless silver-plating strategy, forming a dense nanoparticle-like silver shell that provides abundant plasmonic hotspots for SERS enhancement. Under light irradiation, the micromotors exhibited reversible \"on-off\" locomotion, positive phototaxis, and pronounced light-induced clustering. The micromotors reached an average velocity of approximately 6 μm s⁻¹ under 1.2 mW cm⁻² irradiation and could be remotely guided toward target regions by moving the light spot. Benefiting from light-directed accumulation and intimate probe-analyte contact, the Ag@PS micromotor clusters enabled sensitive SERS detection of rhodamine 6G down to 10⁻⁸ M with good signal reproducibility. Furthermore, the platform achieved label-free in situ Raman detection of diverse microorganisms, including Paramecium, Spirogyra, Euglena, and Marimo, using handheld Raman systems. This work demonstrates a simple and programmable light-responsive micromotor SERS probe for active target enrichment and on-site biological analysis, offering a promising strategy for portable, real-time, and spatially controlled SERS detection.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical applications of molecularly imprinted polymers: a personal view.","authors":"Aysu Yarman, Aysel Oktay, Emine Sezer, Sinan Akgol, Anne Ettner, Zeynep Sabahat Yunt Ghanbari, Cem Bulent Ustundag, Frieder W Scheller","doi":"10.1007/s00216-026-06800-y","DOIUrl":"https://doi.org/10.1007/s00216-026-06800-y","url":null,"abstract":"<p><p>Molecularly imprinted polymers (MIPs) are biomimetic counterparts of biomacromolecules that are synthesized by templating the target within a polymeric network. While the affinity of MIPs can compete with that of antibodies, the specific activity of natural enzymes remains superior to that of catalytically active MIPs. Although the mechanisms underlying the generation of antibodies and MIPs are fundamentally different, important principles have been transferred from biology to artificial binders. Templating a fragment of the target instead of the entire molecule or particle simplifies MIP synthesis and reduces costs (epitope imprinting), while hierarchical imprinting improves template orientation and generates more homogeneous binding sites than conventional random imprinting. The generation of anti-idiotypic MIPs by double imprinting follows the concept of anti-idiotypic antibodies, while post-imprinting modifications mimic post-translational modifications. Beyond classical terminal epitopes, recent approaches increasingly utilize internal epitopes, affinity tags, labels, carbohydrates, and surface motifs of viruses and cells as alternative imprinting targets. Recent advances in analytical MIP systems increasingly rely on the integration of nanomaterials to improve signal generation, mass transport, and target accessibility. In this context, nanoparticles, carbon-based nanomaterials, MXenes, and metal-organic frameworks (MOFs) have enabled the development of more efficient and functionally integrated sensing platforms. In parallel, computational rational design, artificial intelligence, and machine learning-assisted signal deconvolution are emerging as powerful tools for optimizing monomer selection, interpreting complex sensor outputs, and enabling multiplex analysis using cross-reactive MIP sensor arrays. Despite clear advantages such as higher stability, lower cost, and applicability to toxic or weakly immunogenic substances, the commercial success of MIPs is still restricted by challenges related to selectivity, reproducibility, and translation into robust real-world analytical platforms. This review discusses the evolution of analytical MIPs from classical molecular recognition materials toward increasingly intelligent biomimetic sensing systems.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From organs to single cells – frontiers in mass spectrometry imaging","authors":"Sven Heiles,&nbsp;Sabine Schulz,&nbsp;Andreas Römpp","doi":"10.1007/s00216-026-06809-3","DOIUrl":"10.1007/s00216-026-06809-3","url":null,"abstract":"","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"418 20","pages":"6589 - 6590"},"PeriodicalIF":4.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}