结直肠癌的糖蛋白组学分析揭示了糖蛋白和聚糖的差异改变。

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Wenqi Zhang, Xiaotong Wang, Shengye Wen, Wei Ge, Jianbo Deng, Zeyang Zhou, Junhong Jiang, Xiaodong Yang, Yan Wang, Shuang Yang
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引用次数: 0

摘要

结直肠癌(CRC)仍然是一个全球性的健康问题,迫切需要更好的早期发现和靶向治疗。我们的糖蛋白组学分析比较了结直肠癌和癌旁组织(PCA)的差异。全球蛋白质组学分析显示,CRC中有更多独特的蛋白质,在不同阶段CRC中表达差异,特别是在蛋白质合成、翻译和降解途径中。尽管整体的蛋白质组学差异,完整的糖肽分析显示CRC和PCA之间的糖基化高度相似,有722个共享的糖位点,2102个共享的糖肽和435个共享的糖蛋白。然而,观察到CRC的n -聚糖组成发生了重大变化:高甘露糖n -聚糖上调,复合n -聚糖减少,包括唾液聚糖和聚焦唾液聚糖。虽然糖基化基序分析显示保守的肽序列,但附着的n -聚糖类型差异显著。KEGG通路分析提示细胞外基质和溶酶体糖蛋白如COL1A1和LAMP1可能通过tgf β1介导的机制调节PI3K/Akt信号通路,可能促进肿瘤进展。这些发现表明,虽然结直肠癌的整体蛋白表达发生了显著变化,但n -聚糖组成的微妙但关键的变化,特别是高甘露糖n -聚糖的增加和复杂n -聚糖的减少,可能通过调节关键信号通路,在肿瘤进展和转移中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Glycoproteomics analysis of colorectal cancer reveals differential alterations of glycoproteins and glycans.

Colorectal cancer (CRC) remains a global health concern, with an urgent need for better early detection and targeted therapies. Our glycoproteomics analysis comparing CRC to paracancerous (PCA) tissue revealed significant alterations. Global proteomic analysis showed more unique proteins in CRC, with differential expression across CRC stages, especially in protein synthesis, translation, and degradation pathways. Despite overall proteomic differences, intact glycopeptide analysis showed high glycosylation similarity between CRC and PCA, with 722 shared glycosites, 2102 shared glycopeptides, and 435 shared glycoproteins. However, a significant shift in CRC's N-glycan composition was observed: an upregulation of high-mannose N-glycans and a decrease in complex N-glycans, including sialoglycans and fucosylated sialoglycans. While glycosylation motif analysis showed conserved peptide sequences, the attached N-glycan types differed remarkably. KEGG pathway analysis suggested that extracellular matrix and lysosomal glycoproteins like COL1A1 and LAMP1 might modulate the PI3K/Akt signaling pathway via TGFβ1-mediated mechanisms, potentially contributing to tumor progression. These findings suggest that while global protein expression changes significantly in CRC, subtle but crucial changes in N-glycan composition, specifically the increase of high-mannose N-glycans and the decrease of complex N-glycans, may play a significant role in tumor progression and metastasis, potentially through modulation of key signaling pathways.

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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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