Tetracycline detection via carbon dots-Fe coordination from Chromolaena odorata using a portable device.

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Analytical and Bioanalytical Chemistry Pub Date : 2025-09-30 DOI:10.1007/s00216-025-06125-2

Nguyen Huynh Dang Khoa, Nguyen Ngoc Nghia, Bui The Huy, Dang Thanh Cong Minh, Tran Nguyen Cam Nhung, Tran Dang Khoa, Hoang Cao Nguyen, Huynh Anh Khoa, Nguyen Tran Nhat Trung, Nguyen Thi Kim Phuong, Nguyen Huu Hieu

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引用次数: 0

Abstract

Tetracycline residues in aquatic ecosystems, resulting from widespread antibiotic use, pose significant risks by fostering antibiotic resistance and threatening ecological and human health. Addressing the need for rapid, sensitive, and portable tetracycline detection, this study presents a novel device utilizing iron-coordinated carbon dots (CDs-Fe) derived from Chromolaena odorata as a peroxidase-mimic nanozyme. Synthesized via a one-step hydrothermal method, the CDs-Fe nanozyme exhibits enhanced catalytic activity, enabling a colorimetric assay with 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide (H₂O₂). Tetracycline detection relies on the nanozyme's peroxidase-like activity, where tetracycline competitively inhibits TMB oxidation, reducing the absorbance at 652 nm due to surface adhesion and substrate competition. The assay achieves a detection limit of 1.34 μM and a linear range of 17 to 67 μM. A portable Arduino-based device, fabricated via 3D printing with smartphone-enabled Bluetooth data reading, was developed for on-site tetracycline monitoring in real water samples, showing recoveries of 95 to 102% and high selectivity against other antibiotics. Validated against UV-vis spectrophotometry, this green-synthesized, cost-effective system offers a sustainable and field-deployable solution for environmental water quality assessment.

查看原文本刊更多论文

利用碳点-铁配位便携式装置检测臭草中的四环素。

由于抗生素的广泛使用，四环素残留在水生生态系统中，通过促进抗生素耐药性和威胁生态和人类健康，构成重大风险。为了满足快速、灵敏、便携的四环素检测需求，本研究提出了一种利用从臭草中提取的铁配位碳点（cd - fe）作为过氧化物酶模拟纳米酶的新装置。通过一步水热法合成的CDs-Fe纳米酶具有增强的催化活性，可以对3,3',5,5'-四甲基联苯胺（TMB）和过氧化氢（H₂O₂）进行比色测定。四环素检测依赖于纳米酶的过氧化物酶样活性，其中四环素竞争性地抑制TMB氧化，由于表面粘附和底物竞争而降低652nm处的吸光度。检测限为1.34 μM，线性范围为17 ~ 67 μM。一种基于arduino的便携式设备，通过3D打印制造，具有智能手机蓝牙数据读取功能，可用于实际水样中的四环素现场监测，显示回收率为95%至102%，对其他抗生素具有高选择性。通过紫外可见分光光度法验证，该绿色合成、经济高效的系统为环境水质评估提供了可持续的、可现场部署的解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.