{"title":"Analysis of metabolites and metabolic pathways of troponin activator in rats using UHPLC-MS","authors":"Mingxue Yang, Xu Cao, Zhaobo Wu, Xiaochen Huang, Aoxin Ma, Yunda Li, Guojun Li, Kaoqi Lian","doi":"10.1007/s00216-025-06031-7","DOIUrl":"10.1007/s00216-025-06031-7","url":null,"abstract":"<div><p>Troponin activators, including Reldesemtiv and Tirasemtiv, represent a class of drugs that enhance the contractility of cardiac muscle and delay the onset of muscle fatigue. Consequently, they were classified as prohibited substances by the World Anti-Doping Agency (WADA) in 2024. This study aimed to develop an ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS)–based method to quantify Reldesemtiv and Tirasemtiv levels in rat urine, blood, and feces over a 72-h period following transgastric administration. Additionally, Compound Discoverer 3.3 was utilized to investigate the metabolites and metabolic pathways of these two drugs in vivo. Twelve metabolites of Reldesemtiv and seven metabolites of Tirasemtiv were identified. Notably, ten of the twelve Reldesemtiv metabolites had not been previously reported in the literature. Using UHPLC-MS, the concentration of each metabolite in urine and blood was measured at various time points, enabling the generation of a metabolic profile. Six metabolites of Reldesemtiv are detectable 72 h after administration, while Tirasemtiv and its metabolite T-M1a were also detectable at the same time point. This analytical method can characterize the metabolic profiles of the drugs and their metabolites, as well as identify unknown and long-lived metabolites in drug testing. These capabilities are critical for the quantitative analysis of troponin activators within complex biological matrices, supporting their study in physiological and doping-control contexts.</p></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5087 - 5104"},"PeriodicalIF":3.8,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junli Shi, Hui Chong, Xiaofei Yang, Guangjie Zhong, Shengnan Wu, Zehao Gu, Qi Tao, Dong-An Wang, Huaiguo Xue, Yi Yang, Hang Yao
{"title":"Real-time monitoring of cell membrane viscosity in a 3D hydrogel cultivation microenvironment using BODIPY-based fluorescent probe","authors":"Junli Shi, Hui Chong, Xiaofei Yang, Guangjie Zhong, Shengnan Wu, Zehao Gu, Qi Tao, Dong-An Wang, Huaiguo Xue, Yi Yang, Hang Yao","doi":"10.1007/s00216-025-06033-5","DOIUrl":"10.1007/s00216-025-06033-5","url":null,"abstract":"<div><p>This study reports a viscosity-responsive fluorescent probe, BODIPY-C, designed to measure viscosity in a 3D live-cell hydrogel microenvironment using a fluorescence lifetime-based method. This method enables real-time monitoring of cell membrane viscosity changes through the fluorescent lifetime signals of the probe. Functionally, the BODIPY-C exhibits microviscosity sensitivity; the non-toxic probe enables long-term tracking with high sensitivity (<i>R</i><sup>2</sup> = 0.99). Structurally, the BODIPY-C features polymerizable vinyl groups that facilitate covalent conjugation to hydrogel networks via UV-initiated polymerization. This design synergistically integrates the molecular specificity of BODIPY-C-based viscosity sensing with the mechanobiology of covalent hydrogel networks. Notably, softer 3D hydrogel microenvironments extend the probe’s fluorescence lifetime due to restricted molecular motion from differential elastic collisions between polymer chains. The covalent anchoring of BODIPY-C within hydrogel networks enables in situ monitoring of viscosity dynamics in encapsulated cells, establishing a promising platform for investigating mechanobiological processes.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"4959 - 4973"},"PeriodicalIF":3.8,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hongying Wang, Zhiyan Li, Wei Yang, Le Chang, Cunling Yan
{"title":"Development of commutable stabilizing control materials to improve external quality assessment of hemoglobin A1c assay","authors":"Hongying Wang, Zhiyan Li, Wei Yang, Le Chang, Cunling Yan","doi":"10.1007/s00216-025-06018-4","DOIUrl":"10.1007/s00216-025-06018-4","url":null,"abstract":"<div><p>Conformational and colloidal stabilities are responsible for the stability of hemoglobin. However, most hemoglobin <sub>A1c</sub> (HbA<sub>1c</sub>) control materials (e.g., reference materials, calibrators, and quality control materials) are unstable and noncommutable. This may be closely related to the breakdown of the balance between the conformational and colloidal stabilities of hemoglobin. Therefore, we developed a new combined formulation buffer to address the causes of conformational and colloidal instabilities and to maintain hemoglobin or HbA<sub>1c</sub> stability. For the commutability of HbA<sub>1c</sub> control materials, we purified hemoglobin to remove plasma proteins, phospholipids, and fats. On the basis of the above design, we prepared two medical concentrations of HbA<sub>1c</sub> control materials for the external quality assessment (EQA) program and then determined the certified values via the reference measurement method. Stability, homogeneity, value transfer, and commutability were evaluated. Moreover, the control material was distributed to 56 laboratories to assess proficiency testing. The HbA<sub>1c</sub> control materials showed good homogeneity and stability and reliable value transfer, and were commutable for routine clinical analyzers. In the EQA program, only one analyzer exceeded the acceptable bias range (± 6.0%), and the mean interlaboratory <i>CV</i>s of the three analyzers exceeded the acceptable range (3.0%), which can be used to assess true values to improve proficiency testing in the EQA program. Therefore, newly developed control materials can be used to standardize the determination of HbA<sub>1c</sub>.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5001 - 5017"},"PeriodicalIF":3.8,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dominik Kau-Wacht, Nelson G C Astrath, Gustavo V B Lukasievicz, Leopold Lindenbauer, Alicja Dabrowska, Karin Wieland, Bernhard Lendl
{"title":"Laser-based mid-IR photothermal spectroscopy of liquids: a new avenue for in-line sensing in process analytical technology.","authors":"Dominik Kau-Wacht, Nelson G C Astrath, Gustavo V B Lukasievicz, Leopold Lindenbauer, Alicja Dabrowska, Karin Wieland, Bernhard Lendl","doi":"10.1007/s00216-025-06000-0","DOIUrl":"https://doi.org/10.1007/s00216-025-06000-0","url":null,"abstract":"<p><p>Access to real-time chemical and physical information is of fundamental importance in modern producing industries, as it is needed for process monitoring and process control. It also enables process optimization, meeting regulatory requirements. This need motivates new developments in process analytical technologies. Optical in-line probes have emerged as powerful tools for non-invasive monitoring using a range of different spectroscopic techniques. In this regard, mid-infrared spectroscopy is of special interest as it can be used to retrieve both qualitative and quantitative information in a non-destructive and label-free manner. Recently, photothermal methods were also developed in the mid-infrared range, providing a high sensitivity and minimal sample preparation, making them ideal for detecting molecular and structural properties of gases, liquids, and in imaging applications. This study explores the application of reflection-based photothermal beam deflection (PTD) and photothermal mirror (PTM) spectroscopy in comparison with established fiber-optic-based attenuated total reflection spectroscopy (ATR) for real-time analysis of solutes in the mid-infrared range. Both techniques use the same ZnS window, incorporated in a flow cell for experimental simplicity and acting as the sensing interface. Furthermore, the presented PTM and PTD techniques also use the same excitation and probe lasers for ease of comparison. The results demonstrate the effectiveness of these techniques in detecting different concentrations of caffeine in chloroform with similar detection limits to previously presented approaches as well as a state-of-the-art commercial fiber-optic-based ATR process spectrometer. The investigated photothermal techniques hold promise for incorporation in a compact probe design void of any mid-IR fibers. This will pave the way for a new generation of rugged, sensitive, and long-term stable mid-IR in-line probes for use in demanding process analytical technology (PAT) applications.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Annika Fechner, Carolin Drees, Wolfgang Vautz, Stefanie Sielemann, Ursula Telgheder, Joachim Franzke, Sebastian Brandt
{"title":"Seamless analysis of liquid samples by coupling a thermal desorption chip with ion mobility spectrometry","authors":"Annika Fechner, Carolin Drees, Wolfgang Vautz, Stefanie Sielemann, Ursula Telgheder, Joachim Franzke, Sebastian Brandt","doi":"10.1007/s00216-025-06023-7","DOIUrl":"10.1007/s00216-025-06023-7","url":null,"abstract":"<div><p>A novel coupling of a miniaturized thermal desorption chip (TDC) with a flexible microtube plasma (FµTP) ionization source and ion mobility spectrometry (IMS) enables direct, solvent-free analysis of a liquid sample. The integrated TDC-FµTP-IMS system combines sample introduction, preconcentration, and time-resolved desorption via adjustable temperature gradients. Quantitative analysis of a ketone mixture demonstrated excellent linearity and detection limits between 34 µg L<sup>−1</sup> and 79 µg L<sup>−1</sup>. In addition, <i>N</i>-hexanoyl homoserine lactone was successfully quantified from a liquid sample without manual preparation. The method provides a fast, sensitive, and selective approach for direct liquid-phase IMS applications.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5037 - 5046"},"PeriodicalIF":3.8,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12402021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Au-doped MoS2 nanozyme with self-cascade catalytic performance for one-step glucose detection application","authors":"Liping Peng, Yumeng Liu, Qingqing Wu, Lu Zhou, Mengmeng Dong, Lijuan Zhong","doi":"10.1007/s00216-025-06013-9","DOIUrl":"10.1007/s00216-025-06013-9","url":null,"abstract":"<div><p>Glucose oxidase (GOx), which specifically catalyzes glucose oxidation, has been widely employed in constructing various biosensors for clinical screening of diseases such as diabetes and tumors. However, current GOx-based glucose detection methods suffer from limitations including high costs and tedious multi-step procedures. In this work, we developed a facile green aqueous-phase synthesis of Au-doped MoS<sub>2</sub> nanomaterials with dual-enzyme activities. The planar structure of MoS<sub>2</sub> serves as an excellent support for Au deposition, ensuring good dispersion stability. Moreover, Au doping endows the material with GOx-like activity, while the hybrid nanostructure exhibits enhanced peroxidase-like activity due to interfacial interactions in the bimetallic system. The as-prepared Au-MoS<sub>2</sub> nanozyme can trigger a self-cascading reaction, enabling one-step colorimetric glucose detection with a wide linear range and an ultralow detection limit of 0.09 mM. More importantly, practical tests using biological samples (including hepatocellular carcinoma cells and diabetic urine samples) demonstrate the excellent potential for real-world applications. This newly developed material significantly reduces both the time and cost of glucose detection while maintaining good stability and reproducibility, showing promising prospects for clinical diagnostics.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 21","pages":"4945 - 4955"},"PeriodicalIF":3.8,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elena A. Zvereva, Olga D. Hendrickson, Boris B. Dzantiev, Anatoly V. Zherdev
{"title":"Enhanced immunochromatographic assay of tetracycline using intrinsic peroxidase-like activity of gold nanoparticles","authors":"Elena A. Zvereva, Olga D. Hendrickson, Boris B. Dzantiev, Anatoly V. Zherdev","doi":"10.1007/s00216-025-06032-6","DOIUrl":"10.1007/s00216-025-06032-6","url":null,"abstract":"<div><p>Tetracycline (TC) is a broad-spectrum antibiotic widely used in animal husbandry that may contaminate foodstuffs. In this study, a highly sensitive immunochromatographic assay (ICA) for TC was developed and applied to test meat samples. The assay utilized an indirect competitive format, with conjugates of anti-species antibodies and gold nanoparticles (GNPs) used to label specific immune complexes. The intrinsic peroxidase-like activity of GNPs was leveraged to enhance assay sensitivity. GNPs catalyzed substrate oxidation, resulting in a colored product that increased the brightness of the analytical zones on the test strips. An optimal substrate solution based on 3,3′,5,5′-tetramethylbenzidine was selected. The limit of TC detection reached was 0.03 ng/mL. The assay duration was 18 min, including the catalytic reaction. Two meat sample preparation procedures were compared. The developed ICA demonstrated TC recoveries in beef ranging from 92 to 113%. The simple, rapid, and sensitive TC detection, combined with the use of GNP labels well-suited to manufacturing technologies, makes the developed ICA an efficient tool for mass screening tests of food products.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5105 - 5114"},"PeriodicalIF":3.8,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xi Lin, Shiyun Hu, Yanxia Li, Lishuang Yu, Lu Huang
{"title":"Enantioseparation by cLC-UV using chiral packed capillary columns with photonic crystal fibers as frits","authors":"Xi Lin, Shiyun Hu, Yanxia Li, Lishuang Yu, Lu Huang","doi":"10.1007/s00216-025-06020-w","DOIUrl":"10.1007/s00216-025-06020-w","url":null,"abstract":"<div><p>The development of novel enantioseparation materials requires an effective testing method that not only is user-friendly, rapid, and reproducible but also minimizes the amount of enantioseparation material needed. In this work, commercially available silica particles coated with cellulose tris(3,5-dimethylphenylcarbamate) were employed as the chiral stationary phase to develop a simple and rapid method for preparing chiral packed capillary columns compatible with conventional CEC-UV or cLC-UV systems. The column fabrication process took only 2 h. These chiral capillary packed columns, utilizing photonic crystal fibers as frits, successfully separated nine chiral compounds. Among these, six compounds achieved complete resolution within 10 min while demonstrating satisfactory reproducibility and stability. Furthermore, molecular docking simulations were performed using AutoDock and Discovery Studio to investigate the intermolecular interactions between cellulose tris(3,5-dimethylphenylcarbamate) and the nine pairs of enantiomers, providing insights into the enantioseparation mechanism of the chiral stationary phase. This study lays the foundation for the future utilization of the photonic crystal fiber integrated capillary packed column as an advanced platform to explore novel enantioseparation materials.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5027 - 5036"},"PeriodicalIF":3.8,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Davide Barboni, Desiree Bozza, Damiana Natasha Spadafora, Nicoletta Bianchi, Brunilda Myftari, Paola Tedeschi, Chiara De Luca, Simona Felletti, Matteo Spedicato, Alberto Cavazzini, Martina Catani
{"title":"Comparative evaluation of reversed-phase and hydrophilic interaction liquid chromatography columns for untargeted profiling of bioactive compounds in Hypericum perforatum.","authors":"Davide Barboni, Desiree Bozza, Damiana Natasha Spadafora, Nicoletta Bianchi, Brunilda Myftari, Paola Tedeschi, Chiara De Luca, Simona Felletti, Matteo Spedicato, Alberto Cavazzini, Martina Catani","doi":"10.1007/s00216-025-06030-8","DOIUrl":"https://doi.org/10.1007/s00216-025-06030-8","url":null,"abstract":"<p><p>The achievement of a comprehensive profiling of plant metabolites has long represented a challenge, not only due to their wide-ranging abundances but also as a result of their considerable chemical diversity. Recent advances in highly sensitive liquid chromatographic (LC) techniques, particularly when coupled with high-resolution mass spectrometry (HRMS), have established metabolomics as a key approach for the analysis of thousands of non-volatile metabolites in crude natural extracts. Nevertheless, the different polarities of primary and secondary metabolites often limit the efficacy of conventional reversed-phase liquid chromatography (RPLC) in providing exhaustive compound coverage. To address this limitation, orthogonal separation techniques such as hydrophilic interaction liquid chromatography (HILIC) should be employed as a complement to RPLC. In this work, four columns with identical geometrical specifications but with different stationary phase chemistry (one reversed-phase C18 and three different HILIC adsorbents) were employed for the untargeted analysis of bioactive compounds contained in Hypericum perforatum. The columns were evaluated not only in terms of chromatographic performance but also based on their ability to resolve challenging isobaric compound pairs of isobaric compounds. Finally, the integration of RPLC and HILIC data enabled a more comprehensive characterization of the metabolites associated with the plant.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sjors Rasker, Joris Schipperheijn, Stefan Kooij, Marco H. Blokland, Cees J. M. van Rijn, Ane Arrizabalaga-Larrañaga
{"title":"Combining electroless ionisation mass spectrometry with solid-phase extraction for the direct analysis of beta-agonists in bovine urine","authors":"Sjors Rasker, Joris Schipperheijn, Stefan Kooij, Marco H. Blokland, Cees J. M. van Rijn, Ane Arrizabalaga-Larrañaga","doi":"10.1007/s00216-025-06019-3","DOIUrl":"10.1007/s00216-025-06019-3","url":null,"abstract":"<div><p>A novel method combining electroless ionisation mass spectrometry (ELI-MS) with solid-phase extraction (SPE) cartridges enables direct analysis of beta-agonist residues in bovine urine (SPE-ELI-MS). Using a syringe plunger and a battery-powered DIY syringe pump mounted on an XYZ-linear stage, rapid sample clean-up and precise positioning were achieved. Optimisation of spray solvent and nozzle placement improved performance, yielding high sensitivity, repeatability, linearity, and trueness with internal standard correction. This approach offers a simple, efficient solution for analysing organic residues in biological samples, with strong potential for future on-site applications.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5019 - 5025"},"PeriodicalIF":3.8,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12402018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}