Analytical and Bioanalytical Chemistry最新文献

{"title":"Surface characterization of curcumin-visualized latent fingerprints on metal surfaces.","authors":"Kristýna Havelková, Jaroslav Otta, Miroslava Trchová, Tomáš Tobrman, Petr Vrablic, Gabriela Broncová","doi":"10.1007/s00216-026-06445-x","DOIUrl":"https://doi.org/10.1007/s00216-026-06445-x","url":null,"abstract":"<p><p>Visualization of latent fingerprints (LFPs) on metal surfaces using curcumin (CUR) remains a largely underexplored approach. Here, CUR was applied for LFP visualization on brass, steel, Al, and Cu plates and on real-life metal objects (keys, coins, and knives). Fingerprint quality was assessed by stereomicroscopy and characterized in detail using profilometry, SEM/EDS, and vibrational spectroscopy (IR and Raman). Profilometry and SEM/EDS revealed substrate-dependent differences in CUR layer morphology, roughness, and aggregation, indicating that papillary ridge contrast is governed by interactions between fingerprint residues and the underlying surface. Vibrational spectroscopy confirmed the preferential interaction of CUR with lipid-rich LFP components rather than non-specific adsorption on metal substrates and revealed compositional differences in fingerprints deposited on brass and stainless steel. The practical applicability of CUR on real-life metal objects demonstrated that fingerprint persistence and visibility met the legal requirement for forensic usability in the Czech Republic (> 10 minutiae). Overall, these results demonstrate that, under appropriate surface conditions, CUR provides a reliable and environmentally sustainable strategy for targeted LFP visualization on metal substrates.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Not one-size-fits-all: µ-FTIR and pyrolysis GC-MS for complementary analysis of microplastics in eutrophic surface water.","authors":"Timothy Omara, Barbora Benetková, Ivan Sumerskii, Patrick Ssebugere, Christine Kyarimpa, Solomon Omwoma Lugasi, Thomas Rosenau, Christine Betty Nagawa, Stefan Böhmdorfer","doi":"10.1007/s00216-026-06446-w","DOIUrl":"https://doi.org/10.1007/s00216-026-06446-w","url":null,"abstract":"<p><p>This study reports large microplastics found in Lake Victoria through an analytical workflow that combines the complementary methods stereomicroscopy, micro-Fourier transform infrared (µ-FTIR) spectroscopy and pyrolysis gas chromatography-mass spectrometry (Pyr-GC-MS) for the quantification of 11 environmentally relevant microplastic polymers. Algae-rich surface water samples (n = 18) were trawled using a 0.3 mm manta net from Lake Victoria, the world's largest tropical lake. Stereomicroscopy as a determinant analytical technique detected 191 particles, which were primarily blue fragments and fibres. Polyethylene and polypropylene were the dominant polymers identified by µ-FTIR. Pyr-GC-MS allowed the detection and quantification of microplastics (MPs) with LOD and LOQ of 0.01-14.7 µg and 0.03-49.1 µg. Polyethylene (0.058-0.34 µg/L), polypropylene (0.024 µg/L and 0.043 µg/L), nylon 6 (0.0051-0.064 µg/L), nylon 66 (0.0022-0.084 µg/L), poly(ethylene terephthalate) (0.0029-0.027 µg/L) and poly(methyl methacrylate) (0.0036 µg/L) were quantified. µ-FTIR was found to be suitable for the identification of the most abundant polymers in the 0.3-4.9 mm size range whereas Pyr-GC-MS afforded the quantification of seven polymers, most of which were not detected by µ-FTIR. This complementary workflow gave a wider perspective on MP loading, providing both polymer concentrations and physical characteristics (sizes, colours, forms and count) of the MPs.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ICP-MS/colorimetric dual-mode detection of circulating tumor cells with dual-aptamer recognition based on gold nanoparticles.","authors":"Yating Chen, Lina Zheng, Jinchao You, Jinfa Chen, Xiaohao Gan, Yujia He, Wenxiang Wang, Ye He","doi":"10.1007/s00216-026-06451-z","DOIUrl":"https://doi.org/10.1007/s00216-026-06451-z","url":null,"abstract":"<p><p>The efficient identification of circulating tumor cells (CTCs) is vital for early cancer diagnosis and treatment efficacy surveillance. Therefore, developing a CTC detection platform that combines high sensitivity with operational simplicity holds significant value. Dual-mode analytical strategies enhance detection reliability through signal cross-validation, offering a novel approach for constructing such platforms. To this end, this study developed an ICP-MS/colorimetric dual-mode sensing platform incorporating dual-aptamer recognition and gold nanoparticle signal labeling for the precise and sensitive detection of MCF-7 cells, which served as a CTC model. The platform employs magnetic nanoparticles for efficient separation, while gold nanoparticle labeling enables both quantitative ICP-MS analysis and colorimetric readout. Under optimized conditions, the assay achieved a linear range of 200-10,000 MCF-7 cells, with detection limits of 8 cells for ICP-MS and 76 cells for the colorimetric method. In real human blood samples, the spike recovery rate reached 96.90-109.33%, indicating good accuracy and anti-interference capability. The dual-mode strategy proposed in this study integrates high sensitivity with operational convenience. This combination provides a reliable and flexible analytical platform for CTC liquid biopsy and early tumor diagnosis in clinical settings.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of isotope-dilution mass spectrometry, internal standard, and external standard methods for PFAS quantification in tap water and soil.","authors":"Eriko Yamazaki, Nobuyasu Hanari, Keisuke Nakamura","doi":"10.1007/s00216-026-06447-9","DOIUrl":"https://doi.org/10.1007/s00216-026-06447-9","url":null,"abstract":"<p><p>Accurate quantification of per- and polyfluoroalkyl substances (PFAS) in environmental matrices is crucial for reliable exposure assessment but remains challenging owing to low concentrations and matrix-induced variability. In this study, three calibration approaches, external standard (ES), internal standard (IS), and isotope dilution mass spectrometry (IDMS), were systematically evaluated for the determination of perfluorohexanesulfonic acid (PFHxS), perfluorooctanesulfonic acid (PFOS), perfluorooctanoic acid (PFOA), and perfluorononanoic acid (PFNA) in fortified tap water and fortified soil. Linearity of calibration curves was assessed across five concentration levels for each analyte. IDMS consistently demonstrated the highest linearity (coefficient of determination R² of 0.999) for all target analytes, followed by IS. ES displayed acceptable linearity, although the performance was slightly lower in soil matrices, with R² ranging from 0.992 to 0.996. Accuracy was evaluated using measured-to-assigned concentration ratios in spiked tap water. IDMS yielded ratios of 0.96-1.07 with small standard deviations, demonstrating high trueness. When IDMS was used as the reference, IS and ES showed matrix- and analyte-dependent biases. In tap water, IS improved agreement with IDMS relative to ES but still overestimated PFOS, PFOA, and PFNA, whereas ES showed the largest positive bias. In soil, IS produced values close to those of IDMS for all analytes, while ES exhibited greater variability and occasional underestimation. Overall, IDMS enabled the most consistent and reliable quantification across matrices. These results highlight the importance of isotope dilution calibration using structurally matched mass-labeled standards and indicate that IS and ES methods require careful consideration of matrix effects and surrogate selection.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147490212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput microfluidic fluorescent aptasensor platform integrating dual carbon dots and Zn-TCPP for rapid detection of zearalenone.","authors":"Yanjun Yin, Hailin Shen, Jiayi Sun, Zhiguang Suo","doi":"10.1007/s00216-026-06439-9","DOIUrl":"https://doi.org/10.1007/s00216-026-06439-9","url":null,"abstract":"<p><p>Zearalenone (ZEN) is a mycotoxin widely present in crops, posing a serious threat to human health. Therefore, it is of great significance to establish a rapid and accurate method for zearalenone detection. In this work, a microfluidic device was produced using 3D printing technology and employed to construct a high-throughput fluorescence analysis platform. The blue fluorescent carbon quantum dots (B-CDs) and green fluorescent carbon quantum dots (G-CDs) were synthesized and incorporated into separate detection areas to achieve different signal responses. The B-CDs-cDNA/Apt1 and G-CDs-Apt2 complexes are pre-positioned in this area. The two-dimensional layered nanomaterial Zn-TCPP is used as an efficient quencher, achieving specific quenching by its adsorption on single-stranded DNA. Finally, high-throughput rapid detection was achieved by identifying RGB values via a smartphone. Under optimal conditions, the detection range of the fluorescent aptasensor is 0.5-500 ng/mL, with a limit of detection of 1.325 pg/mL. Moreover, this high-throughput fluorescent aptasensor demonstrates excellent analytical performance in the detection of real samples. This work provides a novel strategy for the rapid detection of zearalenone.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of trap column properties and loading conditions for proteome profiling of single-cell-level sample inputs.","authors":"Siqi Huang, Thy Truong, Chao Wang, Xiaofeng Xie, H-J Lavender Lin, Ryan T Kelly","doi":"10.1007/s00216-026-06440-2","DOIUrl":"10.1007/s00216-026-06440-2","url":null,"abstract":"<p><p>Nanoflow liquid chromatography-mass spectrometry has become indispensable for profiling limited and heterogeneous biological samples, yet overall analytical sensitivity, robustness, and throughput often remain constrained by the mechanisms used to transfer samples onto the analytical column. Although trap-and-elute workflows are widely deployed to improve loading efficiency, sample cleanup, and column longevity, a systematic evaluation of how trap column properties and loading parameters influence proteome coverage, particularly under low-input and high-throughput conditions, is needed. Here, we assess trap column inner diameter, particle size, packing material, loading flow rate, and sample concentration using both bulk-prepared digests and single cells. We demonstrate that incorporating a trap column markedly mitigates performance losses associated with large-volume loading with minimal impact on peak width, peak area, or identification depth. Notably, variations in trap column geometry and loading speed exert only minimal influence on chromatographic quality or proteome depth, indicating that trap-and-elute workflows afford considerable flexibility in the LC system design. These findings establish practical guidelines for optimizing trap column configurations and highlight the suitability of trap-and-elute strategies for high-sensitivity, high-throughput proteomics.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13118651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-signal and dual-function probe Ni-MOF@Fe-CDs for sensitive and visual detection of uric acid","authors":"Chunyan Yan,&nbsp;Bowen Yu,&nbsp;Zhengyue Xiao,&nbsp;Panrui He,&nbsp;Di Gao,&nbsp;Xiaomin Tang,&nbsp;Ping Qiu","doi":"10.1007/s00216-026-06422-4","DOIUrl":"10.1007/s00216-026-06422-4","url":null,"abstract":"<div><p>In recent years, the development of nanozyme technology has provided new solutions, but some problems such as insufficient catalytic activity and limited multitarget detection capabilities still need to be addressed. Herein, iron-carbon dots (Fe-CDs) were loaded onto the petals of typical nanoflowery Ni-MOFs, and Ni-MOF@Fe-CDs with excellent catalytic performance and fluorescence characteristics were successfully prepared. Electron paramagnetic resonance (EPR) indicates that •OH is the key intermediate formed during the highly efficient catalytic process of H₂O₂ by Ni-MOF@Fe-CDs. The steady-state kinetic results show the nanozymes exhibit excellent peroxidase-like activity with <i>K</i>_m of 1.18 mM and <i>V</i>_max of 9.82 × 10^–8 M•s⁻¹. The study also found that 2,3-diaminophenazine (DAP) could quench the fluorescence of Ni-MOF@Fe-CDs, and the quenching mechanism between them was further investigated in depth. The inner filter effect (IFE) is dominated in the quench process, which is supported by the IFE-induced inhibition efficiency accounting for 85.2% of the total inhibition efficiency. Combined with the nanozyme and <i>o</i>-phenylenediamine (OPD), a highly sensitive dual-mode (colorimetric-ratiometric fluorescence) platform has been successfully developed for the quantitative analysis of H₂O₂ and uric acid (UA), with a detection limit for UA as low as 0.072 μM. In the analysis of UA in actual urine samples, the recovery rate was in the range of 92.2–107.6% (RSD &lt; 2.9%), verifying the accuracy and reliability of the method. Compared with single-mode sensors for UA detection, the dual output signals endowed to the sensing platform constructed in this work provide complementary sensing performance and realize mutual verification of the detection signals. This approach offers a new strategy for rapid and sensitive detection of UA, with significant potential for translational applications.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"418 9","pages":"2939 - 2953"},"PeriodicalIF":3.8,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validated workflows for preparing and characterizing core-stained and surface-labeled fluorescent polymer particles with simple commercial automation tools.","authors":"Abdelouahad El Abbassi, Paul Fürstenwerth, Christian Würth, Isabella Tavernaro, Ute Resch-Genger","doi":"10.1007/s00216-026-06443-z","DOIUrl":"https://doi.org/10.1007/s00216-026-06443-z","url":null,"abstract":"<p><p>Preparation workflows of fluorescent nm- and µm-sized polymer particles used as reporters in fluorescent assays, bioimaging, and sensing studies or calibration tools for fluorescence methods in the life sciences can be time-consuming and labor-intensive. Also, the outcome can be operator dependent. Here, we present simple and cost-efficient automated workflows for dye loading and surface labeling of polystyrene particles (PSP), using a commercial self-programmable pipetting robot. For developing and fine-tuning automated staining workflows, hydrophobic Nile Red (NR) was incorporated into 100 nm, 200 nm, and 1000 nm PSP by an adapted swelling procedure of premanufactured particles in the presence of hydrophobic dyes, thereby confirming its reliability and versatility. Subsequent expansion of our automation concept to the labeling of carboxylated PSP with pH-sensitive 6-aminofluorescein (6-AMF) and aminated PSP with pH-responsive fluorescein isocyanate (FITC) demonstrated its broad applicability. All automated workflows were optimized and validated by gravimetry and spectroscopic measurements with a microtiter plate (MTP) reader in absorption and fluorescence mode to ensure particle recovery and reproducible fluorescence features and determine PSP dye loading and labeling efficiencies. Comparison with the manual fabrication of NR-stained PSP using an established swelling protocol showed that our automation approach utilizing a pipetting robot considerably reduced the variability in particle recovery and dye loading efficiency. Overall, our simple, labor- and time-efficient workflows with inexpensive and broadly available commercial automation tools present attractive alternatives to manual particle loading and labeling and provide the basis for fast parameter screening, parallel processing, and decreased hands-on time.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A stereo-chemically confusing challenge","authors":"Reinhard Meusinger","doi":"10.1007/s00216-026-06368-7","DOIUrl":"10.1007/s00216-026-06368-7","url":null,"abstract":"","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"418 7","pages":"1805 - 1808"},"PeriodicalIF":3.8,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solution to legislative chemistry challenge","authors":"Juris Meija","doi":"10.1007/s00216-026-06364-x","DOIUrl":"10.1007/s00216-026-06364-x","url":null,"abstract":"","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"418 7","pages":"1809 - 1810"},"PeriodicalIF":3.8,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}