Analytical and Bioanalytical Chemistry最新文献

{"title":"Analysis of time-of-flight secondary ion mass spectrometry data of human skin treated with diclofenac using sparse autoencoder.","authors":"Atsumi Shinozaki, Kazuhiro Matsuda, Satoka Aoyagi","doi":"10.1007/s00216-024-05711-0","DOIUrl":"10.1007/s00216-024-05711-0","url":null,"abstract":"<p><p>Methods that facilitate molecular identification and imaging are required to evaluate drug penetration into tissues. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), which has high spatial resolution and allows 3D distribution imaging of organic materials, is suitable for this purpose. However, the complexity of ToF-SIMS data, which includes nonlinear factors, makes interpretation challenging. Therefore, in this study, ToF-SIMS data of a stratum corneum treated with diclofenac were analyzed using machine learning to enable the evaluation of drug distribution. Diclofenac-related mass peaks were identified using autoencoder results, and the degree of penetration was evaluated across 2-20^th stripped tapes. In addition, the permeation pathway was clarified by comparing the secondary ion images of phosphatidylethanolamine (PhEA; a marker of the inside of the cell); cholesterol, which is abundant in cell membranes; and diclofenac. Based on the biomolecule-related ion images showing the penetration pathway of diclofenac applied to the skin, diclofenac penetrates both the extracellular space and inside cells.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1049-1054"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the interaction between alkaline phosphatase and biomacromolecule substrates.","authors":"Yanan Li, Rong Chen, Yidi Wang, Xin Guo, Xiaojing Lin, Li Tong, Dong Yang, Yanxia Yin, Jing Luo","doi":"10.1007/s00216-025-05740-3","DOIUrl":"10.1007/s00216-025-05740-3","url":null,"abstract":"<p><p>Alkaline phosphatase (ALP) is a nonspecific phosphatase, and its interaction with substrates mainly depends on the recognition of phosphate groups on the substrate. Previous enzymatic research has focused mainly on the enzymatic reaction kinetics of the inorganic small molecule p-nitrophenol phosphate (pNPP) as a substrate, but its interaction with biomacromolecule substrates has not been reported. In current scientific research, ALP is often used for molecular cloning, such as removing the 5' termini of nucleic acids. However, no detailed reports on the interactions between ALP and these biomolecules have been published. We used microscale thermophoresis (MST) and isothermal titration calorimetry (ITC) experiments to investigate the affinity of mutant ALP (S102L) from Escherichia coli for biomacromolecule substrates, including double-stranded DNA (dsDNA) and phosphoproteins. We found that S102L ALP has a strong affinity for dsDNA and β-casein. For the first time, the affinity of ALP for the substrate phosphate monoester has been proven to be significantly affected by the nature of its R group (ROP). In summary, we have explained the key factors involved in the interaction between ALP and biomacromolecule substrates from the perspective of affinity, which provides guidance in better understanding ALP.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1531-1541"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of colloidal carbon-based immunoassay for the determination of thiamethoxam in medicinal and edible Lonicerae Japonicae Flos.","authors":"Mengyue Guo, Tongwei Ke, Huiru Zhang, Jing Zhang, Jia'an Qin, Haonan Ruan, Jiao Tian, Chunjiao He, Yawen Luo, Xuhua Qin, Jiaoyang Luo, Meihua Yang","doi":"10.1007/s00216-025-05749-8","DOIUrl":"10.1007/s00216-025-05749-8","url":null,"abstract":"<p><p>Thiamethoxam is one of the top three neonicotinoids found in the environment and in food. The widespread use of thiamethoxam in medicinal and edible herbs threatens their safe use. Therefore, it is particularly important to develop a rapid and simple approach to detect thiamethoxam residues in herbal medicines. In this study, colloidal carbon-based immunochromatographic strip (CNP-ICS) and colloidal gold-based immunochromatographic test strip (GNP-ICS) methods were respectively developed for the determination of thiamethoxam. The cutoff value of CNP-ICS was 0.1 ng mL^-1, which is six times more sensitive than that of the GNP-ICS method (0.6 ng mL^-1). The CNP-ICS method was applied to detect thiamethoxam in medicinal and edible Lonicerae Japonicae Flos (LJF) for the first time, with a limit of detection of 10 ng g^-1. The detection results of thiamethoxam in 26 batches of LJF samples by the CNP-ICS were consistent with those of the LC-MS/MS method. High residue levels (10.16-530.40 ng g^-1) and high detection rates (69.23%) of thiamethoxam in LJF were observed. The CNP-ICS prepared in this study has the merits of low cost, simple preparation, non-toxicity, and high sensitivity, which can be utilized for rapid field screening of thiamethoxam residues in complex matrices such as herbal medicines.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1595-1606"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive quantification of C4 to C26 free fatty acids using a supercritical fluid chromatography-mass spectrometry method in pharmaceutical-grade egg yolk powders intended for total parenteral nutrition use.","authors":"Mark Dennis Chico Retrato, Anh Vu Nguyen, S J Kumari A Ubhayasekera, Jonas Bergquist","doi":"10.1007/s00216-025-05732-3","DOIUrl":"10.1007/s00216-025-05732-3","url":null,"abstract":"<p><p>Free fatty acids (FFAs) are important energy sources and significant for energy transport in the body. They also play a crucial role in cellular oxidative stress responses, following cell membrane depolarization, making accurate quantification of FFAs essential. This study presents a novel supercritical fluid chromatography-mass spectrometry (SFC-MS) method using selected ion recording in negative electrospray ionization mode, enabling rapid quantification of 31 FFAs within 6 min without derivatization. FFAs are identified and quantified using an HSS C18 SB column and a secondary mobile phase consisting of methanol with formic acid by detecting their [M - H]^- ions. Calibration curves showed strong linearity (R² ≥ 0.9910), spanning 1000-12,000 ng/mL for short-chain FFAs and 50-1200 ng/mL for medium- and long-chain FFAs. The method achieves detection limits as low as 1 ng/µL for short-chain FFAs and 0.05 pg/µL for other FFAs per on-column injection. The method demonstrated high accuracy and precision, with bias and coefficients of variation maintained below 15% across five quality control levels. Freeze-thaw and autosampler stability studies confirmed the behavior of matrix-matched standards under optimal storage conditions. The validated method was applied to the analysis of pharmaceutical-grade egg yolk powders, using 13 deuterated FFAs as internal standards (IS) in comparison with heptadecanoic acid (C17:0). Significant variations in FFA quantification using two different IS approaches underscore the importance of selecting an appropriate IS. In summary, this study introduces a reliable and validated SFC-MS method for analyzing FFAs ranging from C4 to C26, requiring minimal sample preparation.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1461-1478"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioreactor contamination monitoring using off-gassed volatile organic compounds (VOCs).","authors":"Angela L Linderholm, Manohar P Bhandari, Eva Borras, Allison Kwon, Flore M Herve, Mitchell M McCartney, Richart W Harper, Nicholas J Kenyon, Cristina E Davis","doi":"10.1007/s00216-024-05720-z","DOIUrl":"10.1007/s00216-024-05720-z","url":null,"abstract":"<p><p>Metabolically active cells emit volatile organic compounds (VOCs) that can be used in real time to non-invasively monitor the health of cell cultures. We utilized these naturally occurring VOCs in an adapted culture method to detect differences in culturing Chinese hamster ovary (CHO) cells with and without Staphylococcus epidermidis and Aspergillus fumigatus contaminations. The VOC emissions from the cell cultures were extracted and measured from the culture flask headspace using polydimethylsiloxane (PDMS)-coated Twisters, which were subjected to thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) analysis. In our initial time points of 1 and 2 h, we detected VOC signatures that differentiated the cultures earlier than traditional plating techniques or visualization methods. Partial least squares-discriminant analysis (PLS-DA) models were built to differentiate the analytes from the CHO cells and S. epidermidis- and A. fumigatus-inoculated CHO cultures. A total of 41 compounds with a variable importance in projection (VIP) score greater than 1 was obtained across the models. Similarly, based on the PLS regression analyses to predict the cell concentration of S. epidermidis (R² = 0.891) and A. fumigatus (R² = 0.375), 15 and 20 relevant compounds were putatively identified, respectively; two known compounds overlapped between the two microbes. Some of the compounds were unidentified and future studies will determine the relationship between the VOCs and the metabolic changes in contaminated cultures.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1165-1176"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A molecularly imprinted ratio fluorescence sensor based on metal-enhanced fluorescence of core-shell structure CaF₂-silver nanoparticle for visual detection of dicamba.","authors":"Jing Xu, Jiechun Wu, Songlian Li, Zhihong Yan, Qing Zhou, Kang Li","doi":"10.1007/s00216-024-05715-w","DOIUrl":"10.1007/s00216-024-05715-w","url":null,"abstract":"<p><p>Although fluorescence analysis methods are widely used in pesticide residue detection, improving their sensitivity and selectivity remains a challenge. This paper presents a novel ratio fluorescence sensor based on the molecular imprinting polymers (MIPs) and metal-enhanced fluorescence for visual detection of dicamba (DIC). Calcium fluoride (CaF₂) quantum dots (QDs) were immobilized on the surface of Ag@MIPs, resulting in a blue fluorescence response signal (Ag@MIPs-CaF₂). The MIPs shell, which possesses a specific recognition capability, serves as an isolation layer to adjust the distance between Ag nanoparticles and CaF₂ QDs for enhancing the fluorescence of CaF₂ QDs by up to 7.1 times under optimal conditions. In the presence of DIC, the blue fluorescence was selectively quenched, while the reference signal red fluorescence from cadmium telluride QDs coated with silicon dioxide (CdTe@SiO₂) remained relatively stable, resulting in a color change from blue to red. The sensor had a detection limit of 0.16 μM for DIC in the range of 1.0 to 50.0 μM, recovery rates of 85.4 to 103.5% for actual samples, and an imprinting factor of 3.28. The 3D finite-difference time-domain simulation revealed that the fluorescence enhancement was due to the local electric field amplification. Therefore, the developed sensing system in this work offers a broad application prospect for visual detection of DIC in food samples.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1139-1152"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient adsorption and detection of steroid hormones in foods through the combination of novel magnetic TAPB-COF materials with click isotope probes.","authors":"Jiaqi Xu, Qianyu Li, Wenrui Li, Di Wu, Yongning Wu, Guoliang Li","doi":"10.1007/s00216-024-05727-6","DOIUrl":"10.1007/s00216-024-05727-6","url":null,"abstract":"<p><p>Matrix effects pose a significant challenge in food analysis for the quantitative analysis of complex food samples. Herein, a novel magnetic covalent organic framework nanocomposite and the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction-based stable isotope labeling (SIL) method were presented for highly selective and sensitive detection of steroid hormones in food samples using HPLC-MS/MS. The nanocomposite, Fe₃O₄@TAPB-COF, with a core-shell structure exhibited high adsorption capacities for steroid hormones. Combined with a SIL method based on the CuAAC click reaction, steroid hormones were accurately quantified in food samples with high sensitivity and selectivity. A pair of SIL agents, N-(2-azidoethyl)aniline (d₀-NAEA) and d₅-N-(2-azidoethyl)aniline (d₅-NAEA), was synthesized to label steroid hormones in the samples and standard solutions, respectively. The labeling reaction is highly specific, and the formation of the derivatives is easily ionized by MS, thus overcoming matrix effects. More surprisingly, the ionization efficiency of steroid hormones increased by a factor of 4 to 56, with matrix effects ranging from 87.3 to 99.3%. Under optimal conditions, this method exhibited a low limit of detection (LOD) ranging from 0.1 to 2.6 μg L^-1 and overcame the interference of matrix effects for trace-level steroid hormone analysis in foodstuffs.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1199-1212"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of an indirect competitive lateral flow immunoassay for the detection of acetaminophen (paracetamol) in bovine urine.","authors":"Samantha Sasse, Ariadni Geballa-Koukoula, Toine F H Bovee","doi":"10.1007/s00216-024-05721-y","DOIUrl":"10.1007/s00216-024-05721-y","url":null,"abstract":"<p><p>Paracetamol (PCM) is a commonly used analgesic and antipyretic agent for humans worldwide. However, PCM overdoses or overuse can cause health issues, such as hepatoxicity. As PCM is also used for the treatment of farm animals, it is essential to monitor these residues in animal-derived matrices at risk-based sites in order to minimize the intake of PCM through the food chain. In the present study, we have developed a novel carbon nanoparticle (CNP)-based indirect competitive lateral flow immunoassay (icLFIA) for the rapid detection of PCM in bovine urine. The developed icLFIA can detect PCM residues within 10 min, and its performance was validated according to Commission Implementing Regulation (EU) 2021/808, i.e., determination of the detection capability (CCβ), specificity, robustness, and stability. The CCβ of the icLFIA for PCM in bovine urine is 5 mg/L and the icLFIA is proven to be selective and specific towards PCM in bovine urine, as no matrix interference and cross-reactivity were observed, except for high concentrations of orthocetamol. The icLFIA for PCM in bovine urine is robust to (small) variations in reading time, but it remains necessary to strictly use a dilution ratio of running buffer/bovine urine of 80/20. Moreover, the produced icLFIAs are stable for at least 56 days under the stored conditions. In conclusion, the developed and validated icLFIA provides a rapid and cost-effective method for on-site monitoring of PCM abuse in cattle.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1177-1186"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11802664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of membrane porosity in passive sampling of aquatic contaminants for stable isotope analysis: enhancement of analyte accumulation rates and selectivity.","authors":"Armela Tafa, Anat Bernstein, Martin Elsner, Rani Bakkour","doi":"10.1007/s00216-025-05756-9","DOIUrl":"10.1007/s00216-025-05756-9","url":null,"abstract":"<p><p>Compound-specific isotope analysis (CSIA) is a potent method for illustrating the in situ degradation of aquatic contaminants. However, its application to surface and groundwater is hindered by low contaminant concentrations, typically in the nanogram-per-litre range, requiring the processing of large water volumes. Polar organic chemical integrative samplers (POCIS) have shown promising results when combined with CSIA, yet their extended deployment time to accumulate sufficient analyte mass remains a major limitation. In our study, we addressed this issue by increasing the pore size of the polyethersulfone membrane (PES) from 0.1 to 8 <math><mi>μ</mi></math> m. This resulted in significant increases in the mass accumulation rates of atrazine (3.5-fold), S-metolachlor (3.4-fold), and boscalid (3.0-fold). Importantly, the larger pore sizes did not compromise isotopic integrity, with <math><mrow><mi>Δ</mi> <msup><mi>δ</mi> <mn>13</mn></msup> </mrow> </math> C <math><mrow><mo>≤</mo> <mo>+</mo> <mn>0.4</mn> <mo>±</mo> <mn>0.1</mn></mrow> </math> ‰ and <math><mrow><mi>Δ</mi> <msup><mi>δ</mi> <mn>15</mn></msup> </mrow> </math> N <math><mrow><mo>≤</mo> <mo>-</mo> <mn>0.6</mn> <mo>±</mo> <mn>0.4</mn></mrow> </math> ‰, both within accepted uncertainties. Additionally, we observed an enhanced selectivity of the larger pores towards the target analytes over humic acids, whereas no significant increase in (bio)fouling potential was detected for the 8 <math><mi>μ</mi></math> m membrane, as demonstrated by gravimetric analysis, SEM measurements, mass accumulation rates, and isotope ratios of fouled and unfouled POCIS. Our findings show that increasing the membrane pore size from 0.1 to 8 <math><mi>μ</mi></math> m reduces deployment time and expedites the accumulation of analyte mass required for gas chromatography isotope ratio mass spectrometry, offering a promising method to expand CSIA for low-concentration pesticide analysis in the field.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1663-1675"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of predictive modeling tools for the identification of Ocimum spp. herbal products.","authors":"Evelyn J Abraham, Sarah J Chamberlain, Wilmer H Perera, R Teal Jordan, Joshua J Kellogg","doi":"10.1007/s00216-025-05735-0","DOIUrl":"10.1007/s00216-025-05735-0","url":null,"abstract":"<p><p>Species identification of botanical products is a crucial aspect of research and regulatory compliance; however, botanical classification can be difficult, especially for morphologically similar species with overlapping genetic and metabolomic markers, like those in the genus Ocimum. Untargeted LC-MS metabolomics coupled with multivariate predictive modeling provides a potential avenue for improving herbal identity investigations, but the current dearth of reference materials for many botanicals limits the applicability of these approaches. This study investigated the potential of using greenhouse-grown authentic Ocimum to build predictive models for classifying commercially available Ocimum products. We found that three species, O. tenuiflorum, O. gratissimum, and O. basilicum, were chemically distinct based on their untargeted UPLC-MS/MS profiles when grown in controlled settings; combined with an orthogonal high-performance thin-layer chromatography (HPTLC) approach, O. tenuiflorum materials revealed two distinct chemotypes which could confound analysis. Three predictive models (partial least squares, LASSO regression, and random forest) were employed to extrapolate these findings to commercially available products; however, the controlled materials were significantly different from external samples, and all three chemometric models were unreliable in classifying external materials. LASSO was the most successful when classifying new greenhouse samples. Overall, this study highlights how growing and processing conditions can influence the complexity of botanical metabolome profiles; further studies are needed to characterize the factors driving herbal products' phytochemistry in conjunction with chemometric predictive modeling.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1479-1495"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}