Analytical and Bioanalytical Chemistry最新文献_第10页

Quantification of multi-pathway metabolites related to folate metabolism and application in natural population with MTHFR C677T polymorphism. 与叶酸代谢有关的多途径代谢物的定量分析及其在 MTHFR C677T 多态性自然人群中的应用。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-18 DOI: 10.1007/s00216-024-05688-w

Mengdie Wang, Qiwen Zheng, Lei You, Huihui Wang, Peilin Jia, Xinyu Liu, Changqing Zeng, Guowang Xu

{"title":"Quantification of multi-pathway metabolites related to folate metabolism and application in natural population with MTHFR C677T polymorphism.","authors":"Mengdie Wang, Qiwen Zheng, Lei You, Huihui Wang, Peilin Jia, Xinyu Liu, Changqing Zeng, Guowang Xu","doi":"10.1007/s00216-024-05688-w","DOIUrl":"10.1007/s00216-024-05688-w","url":null,"abstract":"Folate, serving as a crucial micronutrient, plays an important role in promoting human growth and supporting transformations to a variety of metabolic pathways including one-carbon, pyrimidine, purine, and homocysteine metabolism. The 5,10-methylenetetrahydrofolate reductase (MTHFR) enzyme is pivotal in the folate metabolic pathway. Polymorphism in the MTHFR gene, especially C677T, was associated with decreased enzyme activity and disturbance of folate metabolism, which is linked to various diseases including birth defects in newborns and neural tube abnormalities. However, the detailed metabolic disturbance induced by MTHFR C677T polymorphism is still elusive. In this study, a novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the precise quantification of 93 metabolites from six important metabolic pathways related to folate metabolism. The method characteristics demonstrated high accuracy and precision, with r2 values ranging from 0.981 to 1.000 for all metabolites. Then the impact of the MTHFR C677T polymorphism on folate metabolism was further investigated, revealing a significant reduction in the level of 5-methyltetrahydrofolate and abnormal levels of metabolites associated with DNA synthesis pathways in individuals carrying the mutation. These data highlight the pivotal role of folic acid supplementation for individuals with the MTHFR C677T polymorphism to mitigate health risks and show the value of precision measurement of folate-related metabolites.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Covalent organic framework derived single-atom copper nanozymes for the detection of amyloid-β peptide and study of amyloidogenesis. 用于检测淀粉样蛋白-β肽和研究淀粉样蛋白生成的共价有机框架衍生单原子铜纳米酶。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-17 DOI: 10.1007/s00216-024-05683-1

Yuxin Wei, Qingqing Bai, Xinlu Ning, Xiaofan Bai, Jie Lv, Meng Li

{"title":"Covalent organic framework derived single-atom copper nanozymes for the detection of amyloid-β peptide and study of amyloidogenesis.","authors":"Yuxin Wei, Qingqing Bai, Xinlu Ning, Xiaofan Bai, Jie Lv, Meng Li","doi":"10.1007/s00216-024-05683-1","DOIUrl":"https://doi.org/10.1007/s00216-024-05683-1","url":null,"abstract":"Sensitive and accurate detection of the amyloid-β (Aβ) monomer is of fundamental significance for early diagnosis of Alzheimer's disease (AD). Herein, inspired by the specific Cu-Aβ monomer coordination, a cutting-edge colorimetric assay based on single-atom Cu anchored N-doped carbon nanospheres (Cu-NCNSs) was developed for Aβ monomer detection and an amyloidogenesis study. By directly pyrolyzing Cu2+-incorporated covalent organic frameworks (COFs), the resulting Cu-NCNSs with a high loading of Cu (8.04 wt %) exhibited outstanding peroxidase-like activity. The strong binding affinity of Aβ monomer to Cu-NCNSs effectively inhibited their catalytic activity, providing the basis for the colorimetric assay. The Cu-NCNSs-based sensor showed a detection limit of 1.182 nM for Aβ monomer, surpassing traditional techniques in terms of efficiency, accuracy and simplicity. Moreover, the system was successfully utilized for Aβ monomer detection in rat cerebrospinal fluid (CSF). Notably, the distinct inhibitory effects of monomeric and aggregated Aβ species on the catalytic activity of Cu-NCNSs were allowed for monitoring of the dynamic aggregation process of Aβ. Compared to thioflavin T (ThT), the most widely used amyloid dye, the detection system exhibited greater sensitivity towards toxic Aβ oligomers, which was crucial for early AD diagnosis and treatment. Our work not only sheds light on the rational design of highly active single-atom nanozymes from COFs but also expands the potential applications of nanozymes in early disease diagnosis.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of the decapping efficiency of THIOMAB™ antibodies with the engineered cysteine in the Fc region for making antibody-drug conjugates by specific hinge fragmentation-liquid chromatography.

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-17 DOI: 10.1007/s00216-024-05707-w

Yun Yang, Jaymin M Patel, Rong-Sheng Yang, Fengfei Ma, Xiangfeng Niu, Yixiao Zhang, Thomas Niedringhaus, Mohammad Al-Sayah, Xiaoyu Yang

{"title":"Determination of the decapping efficiency of THIOMAB™ antibodies with the engineered cysteine in the Fc region for making antibody-drug conjugates by specific hinge fragmentation-liquid chromatography.","authors":"Yun Yang, Jaymin M Patel, Rong-Sheng Yang, Fengfei Ma, Xiangfeng Niu, Yixiao Zhang, Thomas Niedringhaus, Mohammad Al-Sayah, Xiaoyu Yang","doi":"10.1007/s00216-024-05707-w","DOIUrl":"https://doi.org/10.1007/s00216-024-05707-w","url":null,"abstract":"The site-specific antibody-drug conjugates (ADCs), particularly those utilizing the engineered cysteine in Fc fragments of mAbs (THIOMAB™ antibodies), have emerged as a novel class of biotherapeutics for cancer treatment. The engineered cysteine residues in these antibodies are capped by cysteine or glutathione through a disulfide bond. Prior to conjugation with linker-payloads, these caps need to be removed through a reduction process. However, monitoring the efficiency of the decapping process has been challenging due to the lack of effective analytical methods. Intact reversed-phase liquid chromatography-mass spectrometry and hydrophobic interaction chromatography methods failed to separate decapped and capped intact THIOMAB™ mAbs in our study. Instead the fragmentation of mAbs provided a novel strategy to analyze the decapping effiency. After cleavage using a hinge specific enzyme, the generated Fc fragments with and without cysteine and/or glutathione caps displayed different hydrophobicity and were well separated by RPLC, allowing quantitative determination of the decapping efficiency. Enzymes that cleave both above and below the hinge disulfide bonds were tested. The use of FabALATICA can determine percentages of molecules with 0, 1, and 2 cysteine and/or glutathione caps, respectively, regardless of whether the antibody contains the hinge LALA mutations. On the other hand, FabRICATOR enzyme can only be utilized for antibodies without LALA mutations for the overall decapping percentage and cannot be used to estimate intact antibody each with 0, 1, and 2 caps. Therefore, FabALACTICA cleavage followed by RPLC provides a wider application of monitoring the decapping efficiency of all antibodies with the engineered cysteine in Fc.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development, optimization and comparison of solid-liquid and liquid-liquid microextraction for the determination of four flavonols in Schinus molle L. using high-performance liquid chromatography coupled with second-order data modeling. 利用高效液相色谱法和二阶数据模型开发、优化和比较固液微萃取和液液微萃取方法，用于测定 Schinus molle L. 中的四种黄酮醇。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-17 DOI: 10.1007/s00216-024-05700-3

Nicolás A Aschemacher, Álvaro S Siano, Carla M Teglia, Héctor C Goicoechea

{"title":"Development, optimization and comparison of solid-liquid and liquid-liquid microextraction for the determination of four flavonols in Schinus molle L. using high-performance liquid chromatography coupled with second-order data modeling.","authors":"Nicolás A Aschemacher, Álvaro S Siano, Carla M Teglia, Héctor C Goicoechea","doi":"10.1007/s00216-024-05700-3","DOIUrl":"https://doi.org/10.1007/s00216-024-05700-3","url":null,"abstract":"Flavonoids are particularly interesting because they have a broad spectrum of biological effects, including antioxidant and free radical scavenging activities. In this work, solid-liquid microextraction and dispersive liquid-liquid microextraction enhanced by ultrasound were developed and compared with the conventional method (Soxhlet extraction) to optimize the extraction of four flavonoids: rutin, quercitrin, quercetin, and myricetin in samples of Schinus molle (Aguaribay). During the development of the analytical method, different chemometric tools were used to optimize the microextraction procedure. In addition, an analytical method based on high-performance liquid chromatography with diode array detector (HPLC-DAD) and second order calibration using multivariate curve resolution-alternating least square (MCR-ALS) is presented to quantify the flavonoids with limits of quantification between 0.011 and 0.082 µg mL-1. Finally, solid-liquid microextraction using 4.00 mL water/ethanol (54.3:45.7%), 14 s vortex, and 45 min was selected as the most suitable method due to its high recovery rate and environmental friendliness (with a greenness score of 0.78). After the optimization step, the concentrations found in the plant samples were 1825.3, 632.6, 110.2, and 18.9 µg g-1 for rutin, quercitrin, quercetin, and myricetin, respectively. The present work is the first achievement of simultaneously determining these four analytes with exceptional sensitivity, demonstrating lower LOQs compared to previous reports.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Updates implemented in version 4 of the GlyCosmos Glycoscience Portal.

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-17 DOI: 10.1007/s00216-024-05692-0

Sunmyoung Lee, Tamiko Ono, Shiota Masaaki, Akihiro Fujita, Masaaki Matsubara, Achille Zappa, Issaku Yamada, Kiyoko F Aoki-Kinoshita

{"title":"Updates implemented in version 4 of the GlyCosmos Glycoscience Portal.","authors":"Sunmyoung Lee, Tamiko Ono, Shiota Masaaki, Akihiro Fujita, Masaaki Matsubara, Achille Zappa, Issaku Yamada, Kiyoko F Aoki-Kinoshita","doi":"10.1007/s00216-024-05692-0","DOIUrl":"https://doi.org/10.1007/s00216-024-05692-0","url":null,"abstract":"Glycosylation, characterized by its complexity and diversity, is a common system across all domains of life. The glycosylation of proteins or lipids imparts them with structural and functional roles, ranging from development to infectious or Mendelian disease. The high-throughput-based omics data has revealed that glycans are involved in important cellular processes. Comprehensive knowledge of glycosylation has contributed not only to the fundamental concepts in glycoscience but also to its applications, including the development of molecular markers for diagnosis and therapeutic tools for treating diseases. The GlyCosmos Glycoscience Portal (GlyCosmos) has undergone significant updates to better support the scientific community in studying glycosylation-related phenomena. Key enhancements include the integration of expanded datasets linking glycans to other omics fields, improved tools for glycan structure prediction and analysis, and upgraded visualization capabilities to streamline data interpretation. A strengthened focus on data standardization has also been introduced, fostering interoperability between glycoscience resources and external databases. Since its release in 2019, the portal has seen a fivefold increase in user engagement, reflecting its growing relevance. These recent advancements aim to provide researchers with a more comprehensive and user-friendly platform, enabling deeper insights into glycan roles in cellular processes and disease mechanisms. GlyCosmos will continue to evolve, prioritizing community needs and advancing the integration of glycoscience with broader biological and biomedical research.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of RT-dPCR method and reference material for rotavirus G3P8 and G9P8. 开发轮状病毒 G3P8 和 G9P8 的 RT-dPCR 方法和参考材料。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-16 DOI: 10.1007/s00216-024-05690-2

Jiayi Yang, Mingwei Liu, Huijie Li, Yunhua Gao, Lianhua Dong

引用次数: 0

Inter-tissue glycan heterogeneity: site-specific glycoform analysis of mouse tissue N-glycoproteomes using MS1-based glycopeptide detection method assisted by lectin microarray.

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-16 DOI: 10.1007/s00216-024-05686-y

Chiaki Nagai-Okatani, Azusa Tomioka, Daisuke Tominaga, Hiroaki Sakaue, Atsushi Kuno, Hiroyuki Kaji

{"title":"Inter-tissue glycan heterogeneity: site-specific glycoform analysis of mouse tissue N-glycoproteomes using MS1-based glycopeptide detection method assisted by lectin microarray.","authors":"Chiaki Nagai-Okatani, Azusa Tomioka, Daisuke Tominaga, Hiroaki Sakaue, Atsushi Kuno, Hiroyuki Kaji","doi":"10.1007/s00216-024-05686-y","DOIUrl":"https://doi.org/10.1007/s00216-024-05686-y","url":null,"abstract":"To understand the biological and pathological functions of protein glycosylation, the glycan heterogeneities for each glycosite in a single glycoprotein need to be elucidated depending on the type and status of cells. For this aim, a reliable strategy is needed to compare site-specific glycoforms of multiple glycoprotein samples in a comprehensive manner. To analyze this \"inter-heterogeneity\" of samples, we previously introduced an MS1-based glycopeptide detection method, \"Glyco-RIDGE.\" Here, to elucidate inter-tissue glycan heterogeneities, this estimation method was applied to site-specific N-glycoforms of glycoproteins from six normal mouse tissues (liver, kidney, spleen, pancreas, stomach, and testis). The analyses of desialylated glycopeptides estimated 11,325 site-specific N-glycoforms with 239 glycan compositions at 1260 sites (1122 non-redundant core peptides) in 800 glycoproteins, revealing inter-tissue differences in macro-, micro-, and meta-glycan heterogeneities. To obtain detailed information on their glycan features and tissue distribution, the Glyco-RIDGE results were compared with laser microdissection-assisted lectin microarray (LMD-LMA)-based mouse tissue glycome mapping data deposited on LM-GlycomeAtlas, as well as MS-based mouse tissue glycome data deposited on GlycomeAtlas. This integrated approach supported the certainty of Glyco-RIDGE results and suggested that inter-tissue differences exist in glycan motifs, such as alpha-galactose and bisecting N-acetylglucosamine, in both whole tissue glycomes and specific glycoproteins, Anpep and Lamc1. In addition, the comparison with LMD-LMA-based tissue glycome mapping data suggested the possibility of estimating the tissue distribution of the assigned glycans and glycopeptides. Taken together, these findings demonstrate the utility of an integrated approach using MS assisted by LMA for large-scale analyses.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of a colorimetric biosensor based on Fe₃O₄ nanozymes for food safety detection.

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-13 DOI: 10.1007/s00216-024-05679-x

Ningning Guo, Jia Yang, Yixuan Li, Weiing Wang, Xiwen Liang, Qi Xu, Linna Du, Jing Qin

{"title":"A review of a colorimetric biosensor based on Fe3O4 nanozymes for food safety detection.","authors":"Ningning Guo, Jia Yang, Yixuan Li, Weiing Wang, Xiwen Liang, Qi Xu, Linna Du, Jing Qin","doi":"10.1007/s00216-024-05679-x","DOIUrl":"https://doi.org/10.1007/s00216-024-05679-x","url":null,"abstract":"The issue of food safety poses a significant threat to human health. The colorimetric sensing method offers a highly sensitive response, visualization, and easy operation, making it highly promising for applications in the field of bioanalysis. Fe3O4 nanomaterials not only possess the advantages of a straightforward preparation method, customizable functionalities, and facile surface modification, but also exhibit excellent peroxidase activity. The colorimetric biosensor based on a Fe3O4 nanozyme is highly sensitive and has a low detection limit, making it widely recognized in the field of food safety detection. The review provides a summary of synthesis methods for Fe3O4 nanozymes and discusses the effects of different synthesis methods on their structures. Additionally, the catalytic mechanism of the Fe3O4 nanozyme and the influence of particle size, structure, pH, metal doping, and surface modifications on the peroxide activity are analyzed. Finally, we introduce the application of colorimetric sensors based on Fe3O4 nanozymes in detecting antioxidants, heavy metal ions, pesticides, antibiotics, foodborne pathogen toxins, and other food additives and contaminants. This review is expected to provide reference and inspiration for future research on food safety detection through colorimetric sensors based on Fe3O4 nanozymes.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and optimisation of the biosensor for aspartate aminotransferase blood level determination.

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-12 DOI: 10.1007/s00216-024-05682-2

Daryna Mruga, Sergei Dzyadevych, Oleksandr Soldatkin

{"title":"Development and optimisation of the biosensor for aspartate aminotransferase blood level determination.","authors":"Daryna Mruga, Sergei Dzyadevych, Oleksandr Soldatkin","doi":"10.1007/s00216-024-05682-2","DOIUrl":"https://doi.org/10.1007/s00216-024-05682-2","url":null,"abstract":"This work presents the development and optimisation of an amperometric biosensor for determining aspartate aminotransferase (AST) activity in blood serum, using glutamate oxidase and platinum disc electrodes. AST is a key biomarker for diagnosing cardiovascular and liver diseases. The biosensor's bioselective membrane composition and formation protocol and the working solution (aspartate 8 mM, α-ketoglutarate 2 mM, pyridoxal-5-phosphate 100 µM) were optimised. The sensor demonstrated high selectivity, stability (70% retention over 2 months at - 18 °C), and sensitivity (2.37 nA min⁻1 per 10 U L⁻1), with a dynamic range of 0-500 U L⁻1 and a limit of detection of 1 U L⁻1. Comparative analysis showed the calibration curve method outperforms the standard addition method for AST measurement in serum samples. Additionally, a reference spectrophotometric technique was adapted for AST level determination, showing a strong correlation (r = 0.989) with the biosensor results. This research offers a fast, affordable, and accurate tool for early check-ups of liver and heart conditions. The biosensor's flexibility and ease of use make it suitable for further development into point-of-care testing and personalised healthcare techniques.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amphiphilic self-assembling peptides: formulation and elucidation of functional nanostructures for imaging and smart drug delivery.

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-10 DOI: 10.1007/s00216-024-05650-w

Alice Am, Laura Trapiella-Alfonso, Charlotte Izabelle, Bruno Saubamea, Bich-Thuy Doan, Fanny d'Orlyé, Anne Varenne

{"title":"Amphiphilic self-assembling peptides: formulation and elucidation of functional nanostructures for imaging and smart drug delivery.","authors":"Alice Am, Laura Trapiella-Alfonso, Charlotte Izabelle, Bruno Saubamea, Bich-Thuy Doan, Fanny d'Orlyé, Anne Varenne","doi":"10.1007/s00216-024-05650-w","DOIUrl":"https://doi.org/10.1007/s00216-024-05650-w","url":null,"abstract":"The rational design of self-assembled peptide-based nanostructures for theranostics applications requires in-depth physicochemical characterization of the peptide nanostructures, to understand the mechanism and the interactions involved in the self-assembly, allowing a better control of the objects' physicochemical and functional properties for theranostic applications. In this work, several complementary characterization methods, such as dynamic light scattering, transmission electron microscopy, circular dichroism, Taylor dispersion analysis, and capillary electrophoresis, were used to study and optimize the self-assembly of pH-sensitive short synthetic amphiphilic peptides containing an RGD motif for active targeting of tumor cells and smart drug delivery. The combined methods evidenced the spontaneous formation of nanorods (L = 50 nm, d = 10 nm) at pH 11, stabilized by β-sheets. To complement with imaging properties for diagnosis, a new strategy was developed by designing an optimized peptide sequence to allow for efficient functionalization with a contrast agent, while preserving the self-assembling properties. Co-assemblies of the peptide and its derivatives, after peptide modification with a gadolinium complex, exhibited similar nanorod structures and required properties for drug delivery and imaging applications in vivo.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0