Analytical and Bioanalytical Chemistry最新文献

{"title":"Covalent organic framework derived single-atom copper nanozymes for the detection of amyloid-β peptide and study of amyloidogenesis.","authors":"Yuxin Wei, Qingqing Bai, Xinlu Ning, Xiaofan Bai, Jie Lv, Meng Li","doi":"10.1007/s00216-024-05683-1","DOIUrl":"10.1007/s00216-024-05683-1","url":null,"abstract":"<p><p>Sensitive and accurate detection of the amyloid-β (Aβ) monomer is of fundamental significance for early diagnosis of Alzheimer's disease (AD). Herein, inspired by the specific Cu-Aβ monomer coordination, a cutting-edge colorimetric assay based on single-atom Cu anchored N-doped carbon nanospheres (Cu-NCNSs) was developed for Aβ monomer detection and an amyloidogenesis study. By directly pyrolyzing Cu²⁺-incorporated covalent organic frameworks (COFs), the resulting Cu-NCNSs with a high loading of Cu (8.04 wt %) exhibited outstanding peroxidase-like activity. The strong binding affinity of Aβ monomer to Cu-NCNSs effectively inhibited their catalytic activity, providing the basis for the colorimetric assay. The Cu-NCNSs-based sensor showed a detection limit of 1.182 nM for Aβ monomer, surpassing traditional techniques in terms of efficiency, accuracy and simplicity. Moreover, the system was successfully utilized for Aβ monomer detection in rat cerebrospinal fluid (CSF). Notably, the distinct inhibitory effects of monomeric and aggregated Aβ species on the catalytic activity of Cu-NCNSs were allowed for monitoring of the dynamic aggregation process of Aβ. Compared to thioflavin T (ThT), the most widely used amyloid dye, the detection system exhibited greater sensitivity towards toxic Aβ oligomers, which was crucial for early AD diagnosis and treatment. Our work not only sheds light on the rational design of highly active single-atom nanozymes from COFs but also expands the potential applications of nanozymes in early disease diagnosis.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1081-1092"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced solid-liquid synergistic microextraction of nine bisphenols in serum using polyaniline functionalized metal-organic framework nanocomposites/methyl tert-butyl ether.","authors":"Zirong Lan, Linlin Ma, Yanjun Yu, Jiang Qing, Taoyu Meng, Wenli Zhou, Zhou Xu, Mao Long Chen, Li Wen, Yunhui Cheng, Libing Wang, Li Ding","doi":"10.1007/s00216-025-05752-z","DOIUrl":"10.1007/s00216-025-05752-z","url":null,"abstract":"<p><p>Bisphenols, as a new class of environmental endocrine disruptors (EED), can interfere with the endocrine system of the human body and lead to various diseases. In this study, a novel polyaniline functionalized metal-organic framework (PANI@MIL-101@HF) was synthesized by utilizing hollow fibers (HF) as the the immobilization carrier, and combined with methyl tert-butyl ether (MTBE) for solid-liquid cooperative adsorption to determine bisphenols (BPs) in serum samples. The immobilized adsorbent exhibited excellent high stability and hydrophobicity. Furthermore, the inclusion of amino and benzene rings in PANI enhanced the adsorption efficiency of BPs through π-π and hydrogen bond interactions. Surprisingly, owing to the synergies of size exclusion effect of the MIL-101 and HF, the exclusion rate of protein reached as high as 99.2-99.9%. Based on its excellent adsorption properties and protein exclusion effect, the immobilized adsorbent PANI@MIL-101@HF was successfully used as a new restricted material for the high extraction performance with solid-liquid synergy of nine bisphenols (BPs) in serum samples. The operation process has also become more convenient without centrifuging. Integrated with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), the nine BPs in serum samples have a wide linear range (2-200 ng mL^-1) with low quantitative limits of 0.02 ng mL^-1, and the recoveries ranged from 84.65 to 112.56%. The proposed method could be widely applied in convenient, green, and sensitive detection of endocrine disruptors from serum samples.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1619-1634"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A process monitoring microreactor assembly for real-time reaction analysis using inline near-infrared spectroscopy and chemometrics.","authors":"Lukas Mahler, Pascal Desel, Marcel Sladkov, Andreas Roppertz, Christian Mayer, Martin Jaeger","doi":"10.1007/s00216-025-05779-2","DOIUrl":"10.1007/s00216-025-05779-2","url":null,"abstract":"<p><p>The occurrence of highly exothermic and potential runaway reactions represents a significant hazard to workers, facilities, and the surrounding ecosystem. Due to their high reaction velocity, these reactions often prove difficult to monitor. In many instances, the recorded data result from several single-quantity sensors, such as temperature and pressure sensors. Yet, deviations from the anticipated process, and thus potentially hazardous reaction parameters, may only be identified by the combination of the sensor data, leaving the cause of the deviations unexplained. In this study, a custom-built microreactor in combination with in-line near-infrared spectroscopy and off-line gas chromatography was employed to allow monitoring of the acid-catalyzed esterification of acetic acid and methanol. Using a near-infrared spectrometer in conjunction with partial least squares regression models, reactant and product concentrations could be determined at various residence times and temperatures. This approach enabled the safe and efficient investigation of previously unexamined parameter ranges and the expansion of the design space of experiments. The near-infrared spectra were subjected to principal component analysis classification in order to obtain additional qualitative information. The conversions and space-time yields of the microreactor could be calculated from the obtained data. The values indicated that the microreactor design delivered yields up to twice as high as those of commonly used batch reactors. This study hence demonstrates the efficacy of spectroscopic methods for reaction monitoring in microreactors and their potential for application in micro process analytical technologies.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1431-1439"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of mycophenolic acid in plasma by isotope dilution liquid chromatography-tandem mass spectrometry candidate reference method.","authors":"Jing Lin, Min Shen, Ting Yu, Huimin Wang, Jingjue Pan, Gaipeng Huang, Quanle Li","doi":"10.1007/s00216-025-05750-1","DOIUrl":"10.1007/s00216-025-05750-1","url":null,"abstract":"<p><p>Accurate measurements of plasma mycophenolic acid (MPA) are essential for therapeutic drug monitoring in transplant recipients and autoimmune diseases. The performance of plasma mycophenolic acid routine methods remains highly variable that calls for a candidate reference measurement procedure (cRMP) to improve the standardization of plasma mycophenolic acid measurements. In this study, sample preparation was based on protein precipitation with methanol followed by further dilution. The mycophenolic acid was quantified by the isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) with electrospray ionization in positive ion mode. According to the Clinical and Laboratory Standards Institute (CLSI) documents C62-A and C50-A, the basic analytical performance of the candidate reference method was verified, such as linearity, limit of quantification, matrix effect, precision, accuracy, and uncertainty. Moreover, the candidate reference measurement procedure was compared with the routine liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in a clinical laboratory. Based on the data, the mycophenolic acid in human plasma was well detected by ID-LC-MS/MS. No apparent interferences were found with the mycophenolic acid measurement. The calibration curve for the mycophenolic acid was linear in the concentration range of 0.1-50 μg/mL with a correlation coefficient of 0.9999 under the optimum experimental conditions. This method was sensitive because the low limit of quantitation (LOQ) was 0.05 μg/mL. The recoveries of MPA were 98.11-98.95%. The intra-day and inter-day coefficients of variations (CV) of our method were ≤ 1.53% and ≤ 0.51%, respectively. No obvious matrix effect was observed. There was a good correlation between this method and the clinical routine LC-MS/MS method. To sum up, we established and validated a reliable plasma MPA method using ID-LC/MS/MS. The desirable accuracy and precision of this method enable it to serve as a promising cRMP to improve the standardization for plasma MPA routine measurements.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1607-1617"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of Δ⁸- and Δ⁹-tetrahydrocannabinol metabolites and isomers: a rapid assay in urine by LC-MS/MS.","authors":"Marco Ballotari, Michael T Truver, Nayana A Sojin, Lauren A Agliano, Rhea Parimoo, Jennifer L Hoyer, Amie J Goodin, Deepthi S Varma, Chris W Chronister, Kay Roussos-Ross, Bruce A Goldberger","doi":"10.1007/s00216-025-05738-x","DOIUrl":"10.1007/s00216-025-05738-x","url":null,"abstract":"<p><p>An increasing number of cannabis-related products have become available and entered the market, particularly those containing cannabidiol (CBD) and Δ⁸-tetrahydrocannabinol (Δ⁸-THC). Analytical methods for cannabinoids in urine have been described extensively in the literature. However, methods providing good resolution for distinguishing interferences from THC positional isomers are needed. The aim of this project was to develop and validate a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method to quantitate a broad panel of cannabinoids in authentic urine specimens. The method was optimized to quantitate Δ⁸-THC and Δ⁹-THC, 11-OH-Δ⁸-THC and 11-OH-Δ⁹-THC, Δ⁸-THC-COOH and Δ⁹-THC-COOH, CBD, 7-COOH-CBD, CBG, and CBN, and validated with the guidance of the American Academy of Forensic Sciences Standards Board (ASB) Standard 036. The validated assay was then used to evaluate urine samples collected over various time points from female patients (N = 69) enrolled in a study assessing prevalence of marijuana/CBD use during pregnancy from November 2022 to May 2024. Δ⁸- and Δ⁹- isomers were chromatographically resolved and successfully separated. For all analytes, the lower limit of quantitation (LLOQ) was determined to be 10 ng/mL, and the upper limit of quantitation (ULOQ) was 1000 ng/mL. In the authentic samples, the most frequently detected analyte was Δ⁹-THC-COOH, with a median concentration of 278 ng/mL (n = 38). Δ⁹-THC and 11-OH-Δ⁹-THC were detected with a median concentration of 42.4 ng/mL (n = 5) and 65.7 ng/mL (n = 34), respectively. Δ⁸-THC-COOH was detected in n = 3 specimens, with a median concentration of 25.5 ng/mL. The study provided a rapid assay for the analysis of cannabinoids in urine.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1507-1518"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AuNPs/GO/Pt microneedle electrochemical sensor for in situ monitoring of hydrogen peroxide in tomato stems in response to wounding stimulation.","authors":"Xiaolong Yang, Doudou Huo, Yiran Tian, Xingliu Geng, Liang Xu, Daji Zhong, Rong Zhou, Songzhi Xu, Yali Zhang, Lijun Sun","doi":"10.1007/s00216-024-05728-5","DOIUrl":"10.1007/s00216-024-05728-5","url":null,"abstract":"<p><p>Hydrogen peroxide (H₂O₂) is a critical signaling molecule with significant roles in various physiological processes in plants. Understanding its regulation through in situ monitoring could offer deeper insights into plant responses and stress mechanisms. In this study, we developed a microneedle electrochemical sensor to monitor H₂O₂ in situ, offering deeper insights into plant stress responses. The sensor features a platinum wire (100 µm diameter) modified with graphene oxide (GO) and gold nanoparticles (AuNPs) as the working electrode, an Ag/AgCl wire (100 µm diameter) as the reference electrode, and an untreated platinum wire (100 µm diameter) as the counter electrode. This innovative design enhances sensitivity and selectivity through the high catalytic activity of AuNPs, increased surface area from GO, and the superior conductivity of platinum. Operating at a low potential of -0.2 V to minimize interference, the sensor detects H₂O₂ concentrations from 10 to 1000 µM with high accuracy. In situ monitoring of H₂O₂ dynamics in tomato stems under the wounding stimulation reveals that H₂O₂ concentration increases as the sensor approaches the wound site, indicating localized production and transport of H₂O₂. This approach not only improves H₂O₂ monitoring in plant systems but also paves the way for exploring its generation, transport, and elimination mechanisms.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1067-1079"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of time-of-flight secondary ion mass spectrometry data of human skin treated with diclofenac using sparse autoencoder.","authors":"Atsumi Shinozaki, Kazuhiro Matsuda, Satoka Aoyagi","doi":"10.1007/s00216-024-05711-0","DOIUrl":"10.1007/s00216-024-05711-0","url":null,"abstract":"<p><p>Methods that facilitate molecular identification and imaging are required to evaluate drug penetration into tissues. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), which has high spatial resolution and allows 3D distribution imaging of organic materials, is suitable for this purpose. However, the complexity of ToF-SIMS data, which includes nonlinear factors, makes interpretation challenging. Therefore, in this study, ToF-SIMS data of a stratum corneum treated with diclofenac were analyzed using machine learning to enable the evaluation of drug distribution. Diclofenac-related mass peaks were identified using autoencoder results, and the degree of penetration was evaluated across 2-20^th stripped tapes. In addition, the permeation pathway was clarified by comparing the secondary ion images of phosphatidylethanolamine (PhEA; a marker of the inside of the cell); cholesterol, which is abundant in cell membranes; and diclofenac. Based on the biomolecule-related ion images showing the penetration pathway of diclofenac applied to the skin, diclofenac penetrates both the extracellular space and inside cells.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1049-1054"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of colloidal carbon-based immunoassay for the determination of thiamethoxam in medicinal and edible Lonicerae Japonicae Flos.","authors":"Mengyue Guo, Tongwei Ke, Huiru Zhang, Jing Zhang, Jia'an Qin, Haonan Ruan, Jiao Tian, Chunjiao He, Yawen Luo, Xuhua Qin, Jiaoyang Luo, Meihua Yang","doi":"10.1007/s00216-025-05749-8","DOIUrl":"10.1007/s00216-025-05749-8","url":null,"abstract":"<p><p>Thiamethoxam is one of the top three neonicotinoids found in the environment and in food. The widespread use of thiamethoxam in medicinal and edible herbs threatens their safe use. Therefore, it is particularly important to develop a rapid and simple approach to detect thiamethoxam residues in herbal medicines. In this study, colloidal carbon-based immunochromatographic strip (CNP-ICS) and colloidal gold-based immunochromatographic test strip (GNP-ICS) methods were respectively developed for the determination of thiamethoxam. The cutoff value of CNP-ICS was 0.1 ng mL^-1, which is six times more sensitive than that of the GNP-ICS method (0.6 ng mL^-1). The CNP-ICS method was applied to detect thiamethoxam in medicinal and edible Lonicerae Japonicae Flos (LJF) for the first time, with a limit of detection of 10 ng g^-1. The detection results of thiamethoxam in 26 batches of LJF samples by the CNP-ICS were consistent with those of the LC-MS/MS method. High residue levels (10.16-530.40 ng g^-1) and high detection rates (69.23%) of thiamethoxam in LJF were observed. The CNP-ICS prepared in this study has the merits of low cost, simple preparation, non-toxicity, and high sensitivity, which can be utilized for rapid field screening of thiamethoxam residues in complex matrices such as herbal medicines.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1595-1606"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive quantification of C4 to C26 free fatty acids using a supercritical fluid chromatography-mass spectrometry method in pharmaceutical-grade egg yolk powders intended for total parenteral nutrition use.","authors":"Mark Dennis Chico Retrato, Anh Vu Nguyen, S J Kumari A Ubhayasekera, Jonas Bergquist","doi":"10.1007/s00216-025-05732-3","DOIUrl":"10.1007/s00216-025-05732-3","url":null,"abstract":"<p><p>Free fatty acids (FFAs) are important energy sources and significant for energy transport in the body. They also play a crucial role in cellular oxidative stress responses, following cell membrane depolarization, making accurate quantification of FFAs essential. This study presents a novel supercritical fluid chromatography-mass spectrometry (SFC-MS) method using selected ion recording in negative electrospray ionization mode, enabling rapid quantification of 31 FFAs within 6 min without derivatization. FFAs are identified and quantified using an HSS C18 SB column and a secondary mobile phase consisting of methanol with formic acid by detecting their [M - H]^- ions. Calibration curves showed strong linearity (R² ≥ 0.9910), spanning 1000-12,000 ng/mL for short-chain FFAs and 50-1200 ng/mL for medium- and long-chain FFAs. The method achieves detection limits as low as 1 ng/µL for short-chain FFAs and 0.05 pg/µL for other FFAs per on-column injection. The method demonstrated high accuracy and precision, with bias and coefficients of variation maintained below 15% across five quality control levels. Freeze-thaw and autosampler stability studies confirmed the behavior of matrix-matched standards under optimal storage conditions. The validated method was applied to the analysis of pharmaceutical-grade egg yolk powders, using 13 deuterated FFAs as internal standards (IS) in comparison with heptadecanoic acid (C17:0). Significant variations in FFA quantification using two different IS approaches underscore the importance of selecting an appropriate IS. In summary, this study introduces a reliable and validated SFC-MS method for analyzing FFAs ranging from C4 to C26, requiring minimal sample preparation.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1461-1478"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the interaction between alkaline phosphatase and biomacromolecule substrates.","authors":"Yanan Li, Rong Chen, Yidi Wang, Xin Guo, Xiaojing Lin, Li Tong, Dong Yang, Yanxia Yin, Jing Luo","doi":"10.1007/s00216-025-05740-3","DOIUrl":"10.1007/s00216-025-05740-3","url":null,"abstract":"<p><p>Alkaline phosphatase (ALP) is a nonspecific phosphatase, and its interaction with substrates mainly depends on the recognition of phosphate groups on the substrate. Previous enzymatic research has focused mainly on the enzymatic reaction kinetics of the inorganic small molecule p-nitrophenol phosphate (pNPP) as a substrate, but its interaction with biomacromolecule substrates has not been reported. In current scientific research, ALP is often used for molecular cloning, such as removing the 5' termini of nucleic acids. However, no detailed reports on the interactions between ALP and these biomolecules have been published. We used microscale thermophoresis (MST) and isothermal titration calorimetry (ITC) experiments to investigate the affinity of mutant ALP (S102L) from Escherichia coli for biomacromolecule substrates, including double-stranded DNA (dsDNA) and phosphoproteins. We found that S102L ALP has a strong affinity for dsDNA and β-casein. For the first time, the affinity of ALP for the substrate phosphate monoester has been proven to be significantly affected by the nature of its R group (ROP). In summary, we have explained the key factors involved in the interaction between ALP and biomacromolecule substrates from the perspective of affinity, which provides guidance in better understanding ALP.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1531-1541"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}