{"title":"基于ELISA的高氧化酶样中熵纳米酶灵敏检测白细胞介素-6。","authors":"Lifang Chen, Jiaqi Chen, Ying Lei, Pengcheng Lin, Donglin Cao, Wei Xiao, Liangshan Hu","doi":"10.1007/s00216-025-06054-0","DOIUrl":null,"url":null,"abstract":"<div><p>Interleukin-6 (IL-6) is an acute phase response protein. Its level rapidly increases in infection, trauma, and acute inflammatory states. It usually appears earlier than other inflammatory markers such as C-reactive protein and procalcitonin. Therefore, detecting abnormal IL-6 levels (The clinical reference range is greater than 7 pg mL<sup>−1</sup>) faster can help humans receive timely treatment and recover their health. This article successfully synthesized a medium-entropy oxide nanoenzyme. It does not require the involvement of hydrogen peroxide and has a low Michaelis constant of 0.101 mM. Based on the interaction between La-based medium-entropy oxide (MEO-La) nanoenzyme and secondary antibody, specific detection of IL-6 in enzyme-linked immunosorbent assay is achieved. The concentration of IL-6 in human serum can be qualitatively detected. The detection limit and linear range are 0.03 pg mL<sup>−1</sup> and 5–1000 pg mL<sup>−1</sup>, respectively. This work not only is of great significance for better understanding the unique properties of medium-entropy nanoenzymes but also has enormous potential for biochemical sensing of human health.</p><h3>Graphical Abstract</h3><p>This work develops a medium entropy oxide nanoenzyme for ultrasensitive IL-6 detection, achieving a detection limit of 0.03 pg mL<sup>−1</sup>. The hydrogen peroxide free system has shown great potential in early disease diagnosis and biochemical sensing applications.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5171 - 5186"},"PeriodicalIF":3.8000,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Medium-entropy nanoenzyme with high oxidase-like activity for sensitive detection of interleukin-6 based on ELISA\",\"authors\":\"Lifang Chen, Jiaqi Chen, Ying Lei, Pengcheng Lin, Donglin Cao, Wei Xiao, Liangshan Hu\",\"doi\":\"10.1007/s00216-025-06054-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Interleukin-6 (IL-6) is an acute phase response protein. Its level rapidly increases in infection, trauma, and acute inflammatory states. It usually appears earlier than other inflammatory markers such as C-reactive protein and procalcitonin. Therefore, detecting abnormal IL-6 levels (The clinical reference range is greater than 7 pg mL<sup>−1</sup>) faster can help humans receive timely treatment and recover their health. This article successfully synthesized a medium-entropy oxide nanoenzyme. It does not require the involvement of hydrogen peroxide and has a low Michaelis constant of 0.101 mM. Based on the interaction between La-based medium-entropy oxide (MEO-La) nanoenzyme and secondary antibody, specific detection of IL-6 in enzyme-linked immunosorbent assay is achieved. The concentration of IL-6 in human serum can be qualitatively detected. The detection limit and linear range are 0.03 pg mL<sup>−1</sup> and 5–1000 pg mL<sup>−1</sup>, respectively. This work not only is of great significance for better understanding the unique properties of medium-entropy nanoenzymes but also has enormous potential for biochemical sensing of human health.</p><h3>Graphical Abstract</h3><p>This work develops a medium entropy oxide nanoenzyme for ultrasensitive IL-6 detection, achieving a detection limit of 0.03 pg mL<sup>−1</sup>. The hydrogen peroxide free system has shown great potential in early disease diagnosis and biochemical sensing applications.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":462,\"journal\":{\"name\":\"Analytical and Bioanalytical Chemistry\",\"volume\":\"417 23\",\"pages\":\"5171 - 5186\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical and Bioanalytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00216-025-06054-0\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Bioanalytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00216-025-06054-0","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Medium-entropy nanoenzyme with high oxidase-like activity for sensitive detection of interleukin-6 based on ELISA
Interleukin-6 (IL-6) is an acute phase response protein. Its level rapidly increases in infection, trauma, and acute inflammatory states. It usually appears earlier than other inflammatory markers such as C-reactive protein and procalcitonin. Therefore, detecting abnormal IL-6 levels (The clinical reference range is greater than 7 pg mL−1) faster can help humans receive timely treatment and recover their health. This article successfully synthesized a medium-entropy oxide nanoenzyme. It does not require the involvement of hydrogen peroxide and has a low Michaelis constant of 0.101 mM. Based on the interaction between La-based medium-entropy oxide (MEO-La) nanoenzyme and secondary antibody, specific detection of IL-6 in enzyme-linked immunosorbent assay is achieved. The concentration of IL-6 in human serum can be qualitatively detected. The detection limit and linear range are 0.03 pg mL−1 and 5–1000 pg mL−1, respectively. This work not only is of great significance for better understanding the unique properties of medium-entropy nanoenzymes but also has enormous potential for biochemical sensing of human health.
Graphical Abstract
This work develops a medium entropy oxide nanoenzyme for ultrasensitive IL-6 detection, achieving a detection limit of 0.03 pg mL−1. The hydrogen peroxide free system has shown great potential in early disease diagnosis and biochemical sensing applications.
期刊介绍：
Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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