木质素磺酸盐的分子水平表征:高分辨率质谱法。

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Ilya I. Pikovskoi, Anton V. Ilyin, Dmitry S. Kosyakov
{"title":"木质素磺酸盐的分子水平表征:高分辨率质谱法。","authors":"Ilya I. Pikovskoi,&nbsp;Anton V. Ilyin,&nbsp;Dmitry S. Kosyakov","doi":"10.1007/s00216-025-06037-1","DOIUrl":null,"url":null,"abstract":"<div><p>Lignosulfonates (LSs), a large-scale by-product of sulfite pulping, are currently the most widely produced and utilized lignin derivative. Like other lignins, LSs are characterized by an extremely complex irregular structure, and their distinctive feature is the presence of sulfo groups in the side chains of the phenylpropane structural units. The present study, for the first time, proposes a mass spectrometric (Orbitrap) methodology for characterizing the chemical composition of LSs based on the use of negative ion mode atmospheric pressure ionization techniques and chemometric approaches to the data treatment. The LS ionization efficiency increases in the series ESI&lt;APCI&lt;APPI with a substantial change in the selectivity toward sulfonated or nonsulfonated species which can be detected mainly in ESI and APCI ionization modes, respectively. 1,4-Dioxane or ammonia-doped APPI-HRMS enables the reliable detection of &gt;1000 LS oligomers of both CHO- and CHOS-classes in the widest molecular weight range (up to 1.2 kDa). For complex MS and MS/MS (broadband collision induced dissociation) data processing, the filtering methods based on a modified Kendrick mass defect analysis and van Krevelen elemental ratios visualization were introduced. These allow rapid distinguishing between typical lignin structures, sulfonated oligomers with different sulfonation and unsaturation degree, and impurity components. The developed approach was successfully tested on the both reagent-grade and real technical LS samples and can be further used to trace the processes of chemical modification of lignin in sulfite pulping and rapid characterization of LS preparations for various industrial applications.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5131 - 5143"},"PeriodicalIF":3.8000,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular-level characterization of lignosulfonates: high-resolution mass spectrometry approach\",\"authors\":\"Ilya I. Pikovskoi,&nbsp;Anton V. Ilyin,&nbsp;Dmitry S. Kosyakov\",\"doi\":\"10.1007/s00216-025-06037-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Lignosulfonates (LSs), a large-scale by-product of sulfite pulping, are currently the most widely produced and utilized lignin derivative. Like other lignins, LSs are characterized by an extremely complex irregular structure, and their distinctive feature is the presence of sulfo groups in the side chains of the phenylpropane structural units. The present study, for the first time, proposes a mass spectrometric (Orbitrap) methodology for characterizing the chemical composition of LSs based on the use of negative ion mode atmospheric pressure ionization techniques and chemometric approaches to the data treatment. The LS ionization efficiency increases in the series ESI&lt;APCI&lt;APPI with a substantial change in the selectivity toward sulfonated or nonsulfonated species which can be detected mainly in ESI and APCI ionization modes, respectively. 1,4-Dioxane or ammonia-doped APPI-HRMS enables the reliable detection of &gt;1000 LS oligomers of both CHO- and CHOS-classes in the widest molecular weight range (up to 1.2 kDa). For complex MS and MS/MS (broadband collision induced dissociation) data processing, the filtering methods based on a modified Kendrick mass defect analysis and van Krevelen elemental ratios visualization were introduced. These allow rapid distinguishing between typical lignin structures, sulfonated oligomers with different sulfonation and unsaturation degree, and impurity components. The developed approach was successfully tested on the both reagent-grade and real technical LS samples and can be further used to trace the processes of chemical modification of lignin in sulfite pulping and rapid characterization of LS preparations for various industrial applications.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":462,\"journal\":{\"name\":\"Analytical and Bioanalytical Chemistry\",\"volume\":\"417 22\",\"pages\":\"5131 - 5143\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-08-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical and Bioanalytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00216-025-06037-1\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Bioanalytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00216-025-06037-1","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

摘要

木质素磺酸盐(LSs)是目前生产和利用最广泛的木质素衍生物,是亚硫酸盐制浆的一种大规模副产物。与其他木质素一样,LSs具有极其复杂的不规则结构,其显著特征是在苯基丙烷结构单元的侧链上存在亚砜基团。本研究首次提出了一种基于负离子模式大气压电离技术和化学计量学数据处理方法的质谱(Orbitrap)方法来表征LSs的化学成分。在最宽的分子量范围(高达1.2 kDa)内,ESI1000系列CHO-和chos类LS低聚物的LS电离效率都有所提高。针对复杂质谱和MS/MS(宽带碰撞诱导解离)数据处理,提出了基于改进Kendrick质量缺陷分析和van Krevelen元素比可视化的滤波方法。这些方法可以快速区分典型木质素结构、不同磺化程度和不饱和程度的磺化低聚物以及杂质成分。所开发的方法已成功地在试剂级和实际技术LS样品上进行了测试,并可进一步用于跟踪亚硫酸盐制浆中木质素的化学改性过程,以及用于各种工业应用的LS制剂的快速表征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular-level characterization of lignosulfonates: high-resolution mass spectrometry approach

Lignosulfonates (LSs), a large-scale by-product of sulfite pulping, are currently the most widely produced and utilized lignin derivative. Like other lignins, LSs are characterized by an extremely complex irregular structure, and their distinctive feature is the presence of sulfo groups in the side chains of the phenylpropane structural units. The present study, for the first time, proposes a mass spectrometric (Orbitrap) methodology for characterizing the chemical composition of LSs based on the use of negative ion mode atmospheric pressure ionization techniques and chemometric approaches to the data treatment. The LS ionization efficiency increases in the series ESI<APCI<APPI with a substantial change in the selectivity toward sulfonated or nonsulfonated species which can be detected mainly in ESI and APCI ionization modes, respectively. 1,4-Dioxane or ammonia-doped APPI-HRMS enables the reliable detection of >1000 LS oligomers of both CHO- and CHOS-classes in the widest molecular weight range (up to 1.2 kDa). For complex MS and MS/MS (broadband collision induced dissociation) data processing, the filtering methods based on a modified Kendrick mass defect analysis and van Krevelen elemental ratios visualization were introduced. These allow rapid distinguishing between typical lignin structures, sulfonated oligomers with different sulfonation and unsaturation degree, and impurity components. The developed approach was successfully tested on the both reagent-grade and real technical LS samples and can be further used to trace the processes of chemical modification of lignin in sulfite pulping and rapid characterization of LS preparations for various industrial applications.

Graphical Abstract

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信