Analytical and Bioanalytical Chemistry最新文献

{"title":"Food and environment: advances in separation and sensing methodologies","authors":"Giorgia Purcaro, Chiara Emilia Cordero, Emanuela Gionfriddo","doi":"10.1007/s00216-026-06462-w","DOIUrl":"10.1007/s00216-026-06462-w","url":null,"abstract":"","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"418 10","pages":"2955 - 2957"},"PeriodicalIF":3.8,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a highly sensitive electrochemical immunosensor and lateral flow immunoassay for enrofloxacin using optimized haptens.","authors":"Zhenxian Lin, Jingbo Liu, Hui Liu, Jie Zhang, Jianmei Zhang, Guiliang Zheng","doi":"10.1007/s00216-026-06455-9","DOIUrl":"https://doi.org/10.1007/s00216-026-06455-9","url":null,"abstract":"<p><p>Owing to the rapid expansion of the aquaculture industry, the veterinary antibiotic enrofloxacin (ENR) has become a widespread contaminant of concern in environmental and food safety fields. To enable effective monitoring and control of ENR residues, there is an urgent need to develop accurate and user-friendly immunoassays. The preparation of high-quality antibodies relies critically on rationally designed haptens. In this study, we optimized the hapten structure of ENR using computer-aided molecular modeling and the rigid spacer arm strategy. It was found that the antibody generated from the ENR-2 hapten, which features a benzene ring as the spacer, displayed superior affinity (titer 1:248,000) and specificity. Through systematic screening of coating antigens, ENR-3 was identified as the optimal heterologous antigen, leading to significantly improved assay performance. Based on this selected antibody-antigen pair, an electrochemical immunosensor was constructed by integrating gold nanocages (Au NCs) and manganese-zinc ferrite nanoparticles (Mn-Zn-Fe NPs), which achieved a broad detection range from 0.0003 to 300 ng/mL, outperforming most existing ENR immunosensors. In addition, a lateral flow immunoassay (LFIA) was developed for rapid on-site analysis, delivering results within 15 min with a visual detection limit of 9.37 ng/mL. This study not only enriches hapten design theory but also establishes a robust dual-format detection system for ENR, offering reliable tools for residue monitoring.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted approaches based on GC-Orbitrap-HRMS and two-dimensional GC–MS for the identification of intentionally and non-intentionally added substances from bio-based food contact materials","authors":"Maurizio Piergiovanni,&nbsp;Simone Squara,&nbsp;Marco Fontanarosa,&nbsp;Cristian Maffezzoni,&nbsp;Nicolò Riboni,&nbsp;Antonella Cavazza,&nbsp;Monica Mattarozzi,&nbsp;Federica Bianchi,&nbsp;Michele Suman,&nbsp;Maria Careri","doi":"10.1007/s00216-026-06444-y","DOIUrl":"10.1007/s00216-026-06444-y","url":null,"abstract":"<div><p>The increasing adoption of bioplastics is rapidly expanding in food contact materials (FCMs) to minimise environmental impact and improve consumers’ acceptance. However, several aspects must be considered throughout their lifecycle, from bioplastic processing to final product application. This study presents an untargeted analytical workflow to characterise volatile compounds of bioplastic FCMs based on GC–Orbitrap HRMS coupled with chemometrics and complemented by GC × GC-TOF–MS. The bio-based FCMs included polylactic acid (PLA), its heat-resistant variant crystallised PLA (cPLA) and no-PLA materials. The approach based on GC-Orbitrap HRMS coupled to supervised PLS-DA and variable importance in projection (VIP) method allowed the identification of differential compounds able to discriminate samples according to material composition and maximum end-use temperature. Chemometric analysis applied to samples classified according to maximum end-use temperature identified cyclic oligomers and hydrocarbons as markers for PLA samples suitable for use up to 40°C. Considering classification by the type of material resistant to moderate temperature (cPLA <i>vs.</i> no-PLA FCM), analysis of the dataset showed almost completely different VIP selection, with some PLA-related molecules (lactic acid, lactate esters, and linear oligomers) characterising cPLA, some hydrocarbons as key variables of the non-PLA class, and two succinate esters present in both the materials suggesting the presence of PLA/polybutylene succinate blends. In addition to polymer-related molecules, plasticisers emerged as chemical additives incorporated into the investigated materials during manufacturing. In addition, GC × GC-TOF–MS successfully identified distinct series of PLA oligomers, citric acid esters, and mineral oil saturated hydrocarbons, with regular chromatographic patterns, supporting structural hypotheses and integrating GC–Orbitrap HRMS findings.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"418 10","pages":"3099 - 3117"},"PeriodicalIF":3.8,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00216-026-06444-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147519526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strand displacement-driven dual-nuclease cascade strategy for preamplification-free and site-specific RNA m⁶A detection.","authors":"Weifeng Zhang, Xiangming Li, Huihui Shi, Jing Liang, Xiao Ma, Xiaoyan Xu, Chuqi Zhang, Mingyue Xiong","doi":"10.1007/s00216-026-06456-8","DOIUrl":"https://doi.org/10.1007/s00216-026-06456-8","url":null,"abstract":"<p><p>Site-specific detection of N⁶-methyladenosine (m⁶A) in RNA remains challenging due to its lack of distinctive chemical reactivity and preservation of canonical base pairing. Here, we report a strand displacement-driven dual-nuclease cascade (SDD) for preamplification-free, site-specific m⁶A detection. The strategy exploits m⁶A-dependent resistance to MazF cleavage at ACA sites, enabling intact target RNA to trigger toehold-mediated strand displacement and form a DSN-cleavable DNA-RNA hybrid. This hybrid activates DSN-mediated target recycling, leading to iterative release of a Cas13a activator strand and amplified fluorescence readout. The SDD assay achieves a limit of detection of 4.24 fM over a linear dynamic range from 1 fM to 10 nM, with high selectivity against unmodified and non-complementary RNAs. Applied to human serum, the method quantifies site-specific m⁶A on the lncRNA MALAT1 and successfully distinguishes osteosarcoma patients from healthy controls. This work presents a practical approach for site-resolved m⁶A analysis without nucleic acid preamplification.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvements in high-yield detection and localisation of cholesterol in murine brains using water cluster beam secondary ion mass spectrometry.","authors":"Matija Lagator, Irma Berrueta Razo, Sadia Sheraz, Nicholas P Lockyer","doi":"10.1007/s00216-026-06448-8","DOIUrl":"https://doi.org/10.1007/s00216-026-06448-8","url":null,"abstract":"<p><p>Cholesterol is a neutral lipid widely implicated in neurological and other physiological processes. However, it is difficult to detect and precisely localise in situ using mass spectrometry imaging techniques due to low sensitivity and matrix suppression by co-localised lipids. Here, we present the application of large water gas cluster ion beams (GCIBs) for cholesterol analysis and localisation in murine brains using secondary ion mass spectrometry (SIMS). Optimal GCIB parameters were established through analysis of cholesterol standards. These clusters were then used to analyse whole brain sections with minimal sample preparation. Molecular images obtained with water clusters show that cholesterol is distributed across the whole brain, with variations in intensity. In addition, large water clusters were shown to simultaneously maximise secondary ion yields while reducing matrix effects for in situ cholesterol analysis. Depth profiling further revealed that different brain regions produced varying sputter yields. This study demonstrates that large water clusters are very well suited for cholesterol analysis in murine brains. Because this approach requires no derivatisation or matrix addition, it is likely to make analysis faster, simpler, and less prone to matrix effects.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encapsulation efficiency determination methods for the analysis of different antigenic peptides within phosphatidylserine-liposomes.","authors":"Irene Latorre, Montserrat Mancera-Arteu, Oumaima El Ouahabi, Míriam Salvadó, Sílvia Rodríguez-Vidal, Victoria Sanz-Nebot","doi":"10.1007/s00216-026-06453-x","DOIUrl":"https://doi.org/10.1007/s00216-026-06453-x","url":null,"abstract":"<p><p>The prevalence of autoimmune diseases (AIDs) is increasing and persists in individuals of all ages as reported by Pisetsky (Nat Rev Nephrol, 2023). Only palliative treatments are currently available as the development of a treatment that selectively eliminates the immune system autoreaction, without affecting its normal functioning, is not an easy task. In this regard, an innovative strategy based on phosphatidylserine-liposomes (PS-Liposomes) loaded with the autoantigenic peptide that triggers the autoimmune attack has demonstrated efficacy in different AIDs as described by Almenara-Fuentes et al. (Nanomedicine 48, 2023) and Pujol-Autonell et al. (PLoS One 10, 2015). Encapsulation efficiency (EE) determination in liposomal therapeutic formulations is considered one of the most important attributes, as the encapsulated drug concentration is usually related to the treatment's effectiveness. In this regard, several methods have been described in the literature for the determination of specific peptides within liposomes. However, the described methodologies cannot usually be applied to other peptides with different properties. In this study, peptides with different lengths and physicochemical properties encapsulated in PS-Liposome formulations were used as models to evaluate several methods for both free and total antigen quantification. Different approaches for liposome lysis and subsequent total or encapsulated antigen quantification were studied. On the other hand, a comprehensive comparison of different centrifugal filters and an ultracentrifugation method was performed for free antigen determination. Identification and quantification of the autoantigenic peptides were performed by reversed-phase high-performance liquid chromatography with ultraviolet detection (RP-HPLC-UV). Results obtained for direct and indirect EE determinations were compared for PS-Liposome formulations, and the best conditions for each peptide were selected.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New comprehensive two-dimensional gas chromatography with sulfur and mass selective detection setup- expanding analytical capabilities for detection of odor active sulfur compounds in food","authors":"Lara Skef,&nbsp;David Asen,&nbsp;Sebastiano Pantò,&nbsp;Erich Leitner","doi":"10.1007/s00216-026-06449-7","DOIUrl":"10.1007/s00216-026-06449-7","url":null,"abstract":"<div><p>Volatile sulfur compounds belong to the group of odor active compounds with very low sensory threshold levels and can influence the aroma of various products either in a positive or a negative way. Due to their high odor potency and their low sensory thresholds, it is critical to identify these substances in complex food matrices. A new instrumental setup based on the combination of a two-dimensional comprehensive gas chromatographic system with simultaneous dual detection based on a sulfur chemiluminescence detector and a time-of-flight mass spectrometer separation and identification was tested. Additionally, two different column configurations were tested and compared for further optimization of separation and selectivity. Three different roasted coffee samples were used to demonstrate the potential of this new instrumental configuration, keeping in mind the volatile and odor active fraction of roasted coffee being among one of the most complex volatile composition when comparing to other food and beverage products.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"418 10","pages":"3087 - 3097"},"PeriodicalIF":3.8,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00216-026-06449-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the performance of weak and strong ion exchange solid-phase extraction and data mining tools to identify congeners and transformation products in municipal wastewaters by non-targeted analysis.","authors":"Emmanuel Eysseric, Christian Gagnon, L Mark Hewitt, Shirley Anne Smyth","doi":"10.1007/s00216-026-06423-3","DOIUrl":"https://doi.org/10.1007/s00216-026-06423-3","url":null,"abstract":"<p><p>Identifying contaminants in complex chemical mixtures requires powerful non-targeted analysis methods and tools, especially when it comes to detecting novel transformation products (TPs) and congeners from mixtures. In this study, we provided a comparison of weak versus strong ion exchange solid-phase extraction (SPE) for non-targeted accurate mass profiling of contaminants from wastewater samples and evaluated two data mining tools-Molecular networking and Compound Class Scoring (CCS)-for identification and annotation of transformation products and congeners across multiple municipal wastewater treatment plants. Weak ion exchange resins paired with hydrophilic-lipophilic balance resins showed superior performance in terms of higher average recoveries of spiked internal standards and provided enhanced sensitivity for key contaminant classes including surfactants, pharmaceuticals, and recreational drugs. This was reflected by both greater peak areas and the number of compound identifications. Using these techniques, >800 contaminants were identified at various levels of confidence. Consumer product additives and pharmaceuticals accounted for most of the contaminants in terms of number of identified compounds and abundance. Over 50 TPs of pharmaceuticals and multiple subfamilies of consumer product additive congeners were identified with molecular networking. Polyoxyethylene congeners-namely alkyphenol ethoxylates and linear alkyl ethoxylates-were found in high quantities using CCS, which uses both data-dependent and data-independent acquisition tandem mass spectrometry (MS²) data. The tool showed strong proficiency for large-scale identification of polyoxyethylene congeners, as over 2000 unique compounds were detected. Despite the abundant MS² information, only 9% of these compounds were identified, which underscores the challenge posed by complex industrial mixtures.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classification and quantification of full vs. empty adeno-associated virus reference capsids via infrared attenuated total reflection spectroscopy.","authors":"Silke Lehner, Robert Stach, Robin Nilson, Stefan Kochanek, Astrid Kritzinger, Harald Sobek, Vjekoslav Kokoric, Boris Mizaikoff","doi":"10.1007/s00216-026-06429-x","DOIUrl":"https://doi.org/10.1007/s00216-026-06429-x","url":null,"abstract":"<p><p>Gene therapy is based on the introduction of nucleic acids into cells using vectors to enable therapeutic benefits. Hereby, adeno-associated virus (AAV) vectors are preferred for in vivo gene delivery to target cells due to their stability, their ability to transduce a broad range of cell types, and the long-term expression of vector-encoded therapeutic genes in slowly or non-replicating cells. However, large-scale production and quality control during and after manufacturing represent significant challenges. Currently, methods for the analysis of AAVs are rather complex, laborious, expensive, and poorly standardized. Most analyses have to be performed off-line and are not suitable for adaptation toward real-time monitoring during the manufacturing process. Optical analytical methods such as infrared attenuated total reflection (IR-ATR) spectroscopy offer a promising alternative serving as a rapid, cost-efficient, and non-destructive analytical technique with potential on-line monitoring capabilities. The present study fundamentally demonstrates the feasibility of IR-ATR spectroscopy to classify and quantify reference standard materials of full vs. empty AAV type 2 virus capsids. Chemometric methods enable rapid and automated processing of the obtained spectra using specifically partial least squares regression (PLS-R) models. Excellent classification results were achieved, unambiguously identifying samples with predominantly empty capsids vs. those containing predominantly full capsids. The obtained results confirm that IR-ATR spectroscopy in combination with multivariate data evaluation strategies is indeed capable of providing information on the presence of genome-containing virus particles along with the corresponding genomic titer. This analytical strategy may potentially translate into a real-world on-line bioreactor process analytical monitoring technology (PAT) augmenting viral vector production scenarios.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ion chromatography high-resolution mass spectrometry-quality assurance by co-injection of internal standards.","authors":"Joana Flottmann, Torsten C Schmidt, Tobias Schips, Ronja Schmidt, Tobias Bader, Rudi Winzenbacher, Wolfram Seitz","doi":"10.1007/s00216-026-06450-0","DOIUrl":"https://doi.org/10.1007/s00216-026-06450-0","url":null,"abstract":"<p><p>Highly polar and mobile organic substances challenge water analysis due to their persistence, high solubility, and incomplete removal during conventional drinking-water treatment. Their ionic or ionisable nature makes them suitable for ion chromatography coupled to high-resolution mass spectrometry (IC-HRMS), enabling direct injection of aqueous samples and retention of small anions. In this study, an IC-HRMS method was developed and validated for 23 highly polar contaminants, including haloacetic acids, pesticide transformation products, and other mobile and persistent compounds. Limits of quantification ranged from 0.03 to 0.37 µg/L in a linear range of 0.01-100 µg/L. Autosampler-based co-injection of stable isotope-labelled internal standards enabled continuous quality control of retention time, signal intensity, and mass accuracy. Retention time deviations over 4 months of measuring remained below 12 s and mass deviations below 2 ppm, demonstrating robust performance. Application to samples along the drinking-water treatment train confirmed the presence of persistent and mobile substances in the low µg/L range, including trifluoroacetic acid, sulfamic acid, cyanuric acid, and chlorothalonil transformation products. These results demonstrate that IC-HRMS with automated co-injection is a reliable tool for routine monitoring of highly polar anionic contaminants in water.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}