Analytical and Bioanalytical Chemistry最新文献

{"title":"Indirect determination of environmental polylactic acid microplastic residues using a single-atom nickel composite electrode","authors":"Yuexin Pei,&nbsp;Xinlong Pei,&nbsp;Ruichao Shang,&nbsp;Zehui Wang,&nbsp;Gang Liang,&nbsp;Long Li,&nbsp;Ying Yue,&nbsp;Hong Zhu,&nbsp;Wenwen Gong","doi":"10.1007/s00216-025-06046-0","DOIUrl":"10.1007/s00216-025-06046-0","url":null,"abstract":"<div><p>The widely accepted application of polylactic acid (PLA) films as agricultural mulch films has led to the accumulation of microplastics (MPs) in soil ecosystems, causing severe white pollution. Developing a convenient method to detect PLA in the environment is essential for pollution control. In this study, single-atom nickel-doped nitrogen on carbon nanosheets (Ni–N-C) combined with gold–palladium nanoparticles (AuPdNPs) enabled highly sensitive lactic acid detection. Through oxidation catalyzed by the single-atom metal, the detection range for lactic acid was 50 μmol·L⁻¹ to 50 mmol·L⁻¹, with a detection limit of 10 μmol·L⁻¹. This approach indirectly quantified PLA in MPs via lactic acid detection on Ni–N-C + AuPdNPs. This is the first electrochemical method to indirectly determine depolymerized polylactic acid for environmental PLA quantification. The detection current showed minimal fluctuation over 7 days and remained unaffected by common soil interferents. It demonstrates strong applicability in real environments by indirectly assessing MP residues through their depolymerization products, avoiding complex separation processes. High PLA recovery rates in water and soil highlight its potential for environmental monitoring. A stable electrochemical detection of microplastic monomers was provided for such detection approaches.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5263 - 5274"},"PeriodicalIF":3.8,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limitations of boronate affinity chromatography for the specific enrichment of fructose-derived early glycation products in protein analytics","authors":"Sebastian Lux,&nbsp;Clara Vogt,&nbsp;Milena Voll,&nbsp;Ralf Hoffmann","doi":"10.1007/s00216-025-06044-2","DOIUrl":"10.1007/s00216-025-06044-2","url":null,"abstract":"<div><p>Protein glycation, or non-enzymatic glycosylation, refers to the reaction of reducing sugars with amino groups in proteins to form Amadori and Heyns products for aldoses (e.g., glucose) and ketoses (e.g., fructose), respectively. While Amadori peptides have been well studied after enrichment by boronate affinity chromatography (BAC), it is often assumed that BAC also enriches the isomeric Heyns peptides, although the binding of Heyns rearrangement products seems unlikely due to the very low content of 1,2- and 1,3-<i>cis</i>-diols in their dominant tautomeric forms. For seven different tryptic peptide sequences derived from human plasma digests, we showed that the synthetic glucose-modified Amadori peptides can be enriched by BAC with high recovery rates, while the corresponding fructose-modified Heyns peptides did not bind, independent of the buffers and pH used. Reduction of the carbonyl groups with borohydride, yielding the corresponding hexitol-modified peptides, allowed enrichment of both the former Amadori and, more importantly, Heyns peptides.</p><h3>Graphical Abstract</h3><p>Molecular interactions of boronic acids with unreduced and reduced Amadori (ARPs) and Heyns rearrangement products (HRPs)</p>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5239 - 5251"},"PeriodicalIF":3.8,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12431902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical dissection of minor glycoforms and glycoprotein associations in rAAV preparations by multimodal glycoproteomics","authors":"Atsushi Kuno,&nbsp;Hiroaki Sakaue,&nbsp;Sachiko Koizumi,&nbsp;Azusa Tomioka,&nbsp;Saho Mizukado,&nbsp;Yuki Yamaguchi,&nbsp;Mitsuko Fukuhara,&nbsp;Yasuo Tsunaka,&nbsp;Hiroyuki Kaji,&nbsp;Susumu Uchiyama","doi":"10.1007/s00216-025-06042-4","DOIUrl":"10.1007/s00216-025-06042-4","url":null,"abstract":"<div><p>Accurate glycan analysis of viral vectors is essential for evaluating pharmaceutical quality. Recent advances in mass spectrometry–based analytical technologies have achieved glycosylation detection in adeno-associated viruses (AAVs). However, because only a minor subpopulation (&lt; 1%) of recombinant AAV (rAAV) particles may carry glycans or associate with glycoproteins, distinguishing genuine AAV glycosylation from that of co-purified glycoproteins remains technically challenging, highlighting the need for analytical strategies that minimize glycan misassignment and reliably identify glycoprotein interactions. Here, we present a multimodal glycoproteomic approach to discriminate rare glycosylation events on rAAV capsids from glycosylated host-derived proteins associated with the particles. We employed an ultrasensitive lectin microarray coupled with a broadly reactive anti-AAV antibody to detect <i>O</i>-glycan-binding lectin signals in several rAAV preparations. Notably, a distinct signal was observed for <i>Urtica dioica</i> agglutinin (UDA). Subsequent liquid chromatography-tandem mass spectrometry, combined with UDA-based dual enrichment at both protein and peptide levels, identified a divalently high-mannose <i>N</i>-glycosylated peptide derived from the host AAV receptor (AAVR). Monovalent high-mannose <i>N</i>-glycopeptides of AAVR and Mac-2 binding protein were additionally detected using single-step protein-level enrichment, indicating an avidity-driven UDA binding mechanism. However, no <i>N</i>-glycosylation was detected on the rAAV capsids themselves. These findings underscore the value of integrated multimodal glycoproteomic workflows for resolving low-abundance glycosylated species and offer new insights into host-derived hitchhiker glycoproteins that may affect rAAV characterization and quality control.</p><h3>Graphical Abstract</h3><p>Estimated <i>Urtica dioica</i> lectin-binding detection. (<b>A</b>) Although recombinant adeno-associated virus (rAAV) does not contain <i>N</i>-glycans, a <i>Urtica dioica</i> agglutinin (UDA) signal is detected on the antibody-overlay lectin microarray, likely due to glycosylated hitchhiker proteins. (<b>B</b>) Peptide-level UDA capture enables efficient detection of a divalent high-mannose glycopeptide derived from the AAV receptor (AAVR) by selectively removing free peptides and low-affinity glycopeptides</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5155 - 5170"},"PeriodicalIF":3.8,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and application of a simple formate-buffered extraction method for the analysis of 104 organic pollutants in river suspended particulate matter by HPLC–ESI–MS/MS","authors":"Alexis P. Roodt,&nbsp;Ralf Schulz","doi":"10.1007/s00216-025-06049-x","DOIUrl":"10.1007/s00216-025-06049-x","url":null,"abstract":"<div><p>Globally, rivers are contaminated by a diverse range of organic micropollutants originating from waste water effluents or surface runoff in urban and agricultural areas. In rivers, suspended particulate matter (SPM), which can be constituted by a high proportion of organic material and fine mineral particles, sorbs many of these organic micropollutants and thus influences their distribution and residence times. Furthermore, benthic organisms, which live and feed in freshly deposited sediments, may be at increased risk of exposure to SPM-associated organic micropollutants. Efficient methods for the analysis of organic micropollutants in river SPM are therefore required for studying the distribution of these pollutants in space and time as well as the potential exposure of benthic organisms. In recent years, the “quick, easy, cheap, effective, rugged and safe” (QuEChERS) sample preparation methodology has gained increasing popularity for the analysis of organic micropollutants in environmental samples, including soils and sediments. In this study, we compared four extraction methods based on the QuEChERS methodology for their performance when combined with high-performance liquid chromatography tandem mass spectrometry analysis. As a result, a simple formate-buffered extraction method for the analysis of 104 selected organic pollutants, including pesticides and pharmaceuticals, in river SPM was developed and validated. The validated method was sensitive, with a median method limit of quantification of 1.0 ng/g. The method was applied to investigate the spatial and temporal distribution of the selected pollutants in SPM samples collected in the French-German border river, Lauter, which is an important regional refuge for biodiversity.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5287 - 5301"},"PeriodicalIF":3.8,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A validated LC–MS/MS multi-method for the determination of 110 mycotoxins and plant toxins in cow milk and application to samples from Germany","authors":"Ahmed H. El-Khatib,&nbsp;Arnold Bahlmann,&nbsp;Christoph Hutzler,&nbsp;Stefan Weigel","doi":"10.1007/s00216-025-06024-6","DOIUrl":"10.1007/s00216-025-06024-6","url":null,"abstract":"<div><p>Milk is widely consumed across all ages and is essential for dairy production. Milk quality and safety are largely influenced by the animal’s diet, which may contain feedborne contaminants. This can lead to multi-toxin exposure, potentially transferring toxins to milk and posing health risks to consumers. Currently, only aflatoxin M1 (AFM1) is regulated by the European Union in milk (maximum level (ML) = 0.05 µg/kg) but the (co-)occurrence of other toxins should also be investigated. In this work, a QuEChERS-based LC–MS/MS multi-method for the simultaneous determination of 72 mycotoxins and 38 plant toxins in raw cow milk was developed and validated according to the latest EU regulations. The method showed excellent recoveries (87% of the analytes have average recoveries within 70–120%) and precision (97% of the analytes have within-laboratory reproducibility ≤20%). Limit of quantification (LOQ) for AFM1 (0.0035 µg/kg) was ≤½ ML. The method was applied to 20 milk samples (15 conventional and 5 organic samples) from retail stores and local farms. The analysis of these milk samples showed the co-occurrence of the emerging mycotoxins beauvericin and enniatin B as well as the quinolizidine alkaloids (QAs) lupanine and 13a-hydroxylupanine in the majority of samples. The pyrrolizidine alkaloid senkirkine was also detected in almost half of the samples. The results revealed generally trace levels of mycotoxins and plant toxins. However, QAs (especially lupanine) were detected at much higher concentration levels than other toxin groups (especially in organic milk samples). The findings showed the co-occurrence of multiple toxins in the range of 2–12 toxins in every sample.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5047 - 5059"},"PeriodicalIF":3.8,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A mitochondrial-targeted fluorescent probe for imaging HOCl in ferroptosis and multiple liver injury models","authors":"Cailing Fan,&nbsp;Na Xiao,&nbsp;Yixing Shi,&nbsp;Yonghao Song,&nbsp;Lei Liu,&nbsp;Yuwei Wu,&nbsp;Keying Yang,&nbsp;Yongwei Limeng,&nbsp;Qinxi Dong,&nbsp;Yanan Gao,&nbsp;Wei Shu,&nbsp;Chaoyuan Zeng","doi":"10.1007/s00216-025-06028-2","DOIUrl":"10.1007/s00216-025-06028-2","url":null,"abstract":"<div><p>Ferroptosis exerts a significant influence on the pathogenesis, progression, and therapeutic outcomes of liver diseases. Mitochondria play pivotal roles in liver disease and are also an indispensable hub in the final implementation of ferroptosis. The overabundance of mitochondrial HOCl can result in the permeabilization of mitochondria, which can cause cell death and damage to liver tissue. Herein, we designed a fluorescent probe named <b>PTZ-PY</b> based on thiophenazine for HOCl rapid detection. <b>PTZ-PY</b> exhibits excellent sensitivity and high selectivity to HOCl, and the LOD of HOCl is 13 nM. <b>PTZ-PY</b> is capable of targeting mitochondria and effectively monitors fluctuations in endogenous HOCl concentrations within cellular and zebrafish models, and has been utilized to document the escalation of HOCl levels associated with ferroptosis. As an efficient tool for detecting HOCl, <b>PTZ-PY</b> has demonstrated remarkable potential in exploring functions of HOCl in physiological processes, suggesting that it holds a promising application prospect in related research fields.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5077 - 5086"},"PeriodicalIF":3.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144787995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic comparison of methods for offline breath sampling","authors":"Mark Woollam,&nbsp;Andrew Christensen,&nbsp;Eray Schulz,&nbsp;Serenidy Eckerle,&nbsp;Michael D. Davis,&nbsp;Don B. Sanders,&nbsp;Mangilal Agarwal","doi":"10.1007/s00216-025-06025-5","DOIUrl":"10.1007/s00216-025-06025-5","url":null,"abstract":"<div><p>Harnessing the potential of exhaled breath analysis is an emerging frontier in medical diagnostics, given breath is a rich source of volatile organic compound (VOC) biomarkers for different medical conditions. A current downfall in this field, however, is the lack of standardized and widely available methods for offline sampling of exhaled VOCs. Herein, strides are taken toward the standardization of breath sampling in Tedlar bags by exploring several factors that can impact VOC heterogeneity, including tubing material, chemical composition of collection bags, breath fractionation, exhalation volume, and transfer flow rate. After bag-based sampling standardization, performance was benchmarked using two offline breath sampling methods, Tedlar bags and the Respiration Collector for In Vitro Analysis (ReCIVA). Three volunteers from the laboratory with no known respiratory diseases donated ≥ <i>n</i> = 5 samples collected onto adsorption tubes via each method, which were analyzed through thermal desorption (TD) coupled with gas chromatography-mass spectrometry (GC–MS). Data processing revealed a set of 15 highly reliable on-breath VOCs detected across volunteers, and most analytes (except indole) demonstrated higher sensitivity using Tedlar bags. Calculating relative standard deviation (RSD) values showed Tedlar bags were also significantly more reproducible compared to the ReCIVA (<i>p</i> &lt; 0.03). Agreement between the two methods was demonstrated through correlating VOC signals with high statistical significance (<i>R</i>² = 0.70), indicating both devices are well situated for biomarker discovery applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5061 - 5076"},"PeriodicalIF":3.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12402010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of a scattered light sensor for monitoring the dispersed surface in multimodal system.","authors":"Lukas Schmitt, Stephan Scholl, Matthias Rädle","doi":"10.1007/s00216-025-06038-0","DOIUrl":"https://doi.org/10.1007/s00216-025-06038-0","url":null,"abstract":"<p><p>The article presented here deals with the quality parameter dispersed surface in material systems like suspensions or dispersions in which several phases are present simultaneously such as solids in continuous solution. Here, other quality parameters are relevant for the quality of the products than, for example, in pure solutions or gases. The dispersed surface is relevant for reaction rates, absorption capacity, and many other properties of the suspension. In contrast to the common method of measuring particle size distributions and solid concentrations in a complex manner, the article shows that the use of a simple scattered light measurement is also sufficient for disperse phase measurement. Based on previous investigations with monodisperse material systems \"Use of a scattered light sensor for monitoring the dispersed surface in crystallization\" (Schmitt et al., Chem. Ing. Tech., Bd. 94, Nr. 8, S. 1177-1184, 2022), the optical effect of mixtures of fine and coarse particles is discussed here. It is shown that the dispersed surface can also be determined directly in materials characterized by very broad particle size distributions using scattered light probes. In addition to the pure measurement results, the description of the measurement setup and derived findings, the article deals with several side aspects of disperse-phase systems. The specific dispersed surface of the dispersed phase in suspensions, emulsions, bubble columns, and aerosols plays a decisive role in increasing heat and mass transfer processes. This has a direct impact on the space-time yield in large-scale chemical process engineering production plants. The results obtained contribute to a comprehensive understanding of the dispersed surface and enable improved control and optimization strategies in the chemical engineering industry.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser-modulated MnBi2Te4 terahertz biosensor for high-sensitivity marine bacterial detection","authors":"Xin Hu,&nbsp;Wenhao Xu,&nbsp;Jing Peng,&nbsp;Baoxin Sun,&nbsp;Zhaoan Shao,&nbsp;Qi Song,&nbsp;Bingyuan Zhang,&nbsp;Hongzhuan Xuan","doi":"10.1007/s00216-025-05996-9","DOIUrl":"10.1007/s00216-025-05996-9","url":null,"abstract":"<div><p>The prompt and precise identification of marine bacteria is essential for assessing ecosystem health and mitigating microbial contamination in aquatic environments. In this study, we introduce a terahertz (THz) biosensor that utilizes magneto-topological MnBi₂Te₄ thin films, capitalizing on their quantum anomalous Hall effect and surface-dominated optoelectronic characteristics for label-free bacterial detection. Through the application of surface engineering and laser modulation techniques, the sensor achieves a detection limit of 9.4 CFU/mL for <i>Ruegeria pomeroyi</i> DSS-3, exhibiting adjustable THz photoresponses under ambient conditions. Notably, distinct THz absorption signatures were identified for the bacterial proteins sulfide-quinone oxidoreductase (SQR) and flavocytochrome <i>c</i> subunit B (FccB), with responsivity values ranging from 0.018 to 0.042 A/W at 0.1 THz. Field validation conducted in natural seawater across various latitudes (20–38°N) demonstrated minimal environmental interference (&lt; 1%), indicating the sensor's robustness in complex marine matrices. This research represents a pioneering effort in the integration of topological materials with THz photonics, providing a biocompatible, non-destructive, and cost-effective platform for real-time biomonitoring in marine environments.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 21","pages":"4791 - 4801"},"PeriodicalIF":3.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an immunoassay lollipop using syringe-autoinjected visual distance readout for point-of-care testing","authors":"Cuili Li,&nbsp;Maojie Xiang,&nbsp;Jie Liu,&nbsp;Shuting Zhang,&nbsp;Haojie Li,&nbsp;Guanglei Fu","doi":"10.1007/s00216-025-06039-z","DOIUrl":"10.1007/s00216-025-06039-z","url":null,"abstract":"<div><p>Pressure-based detection has drawn increasing interest for developing immunoassays but is still challenged in point-of-care testing (POCT). In this work, a pressure-based immunoassay “lollipop” was developed using syringe-autoinjected visual distance readout. The “lollipop” consisted of a disposable syringe “stick” as the immuno-recognition host and a chip “head” as the signal readout element. Using urinary microalbuminuria (mALB) as a model target, we constructed the immunoassay system on the interior cylinder surface of the syringe. In the presence of H₂O₂, the peroxidase-mimicking activity of platinum nanoparticles (Pt NPs) immuno-captured in the cylinder drove the automatic injection of dye liquids from the syringe into a spiral capillary catheter in the chip. The concentration of mALB in the “lollipop stick” was thereby visually displayed as the syringe-autoinjected distance signals in the “lollipop head”. The assay exhibited a limit of detection (LOD) of 5 ng/mL for mALB quantification and linear response in a range from 0.006 μg/mL to 6 μg/mL with reliable accuracy in clinical urine sample analysis. Taking advantage of the versatility of syringes, the immunoassay “lollipop” works without the aid of any laboratory liquid handlers (e.g., pipettes), specific detectors (e.g., manometers) and complex chip operations (e.g., reagent loading), showing great promise in POCT.</p></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5145 - 5154"},"PeriodicalIF":3.8,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}