Analytical and Bioanalytical Chemistry最新文献_第9页

Synthesis and characterization of molecularly imprinted polymer nanoparticles against porcine circovirus type 2 viral-like particles. 针对猪圆环病毒 2 型病毒样颗粒的分子印迹聚合物纳米粒子的合成与表征。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-11 DOI: 10.1007/s00216-024-05576-3

Jutapak Klangprapan, Wisnu Arfian A Sudjarwo, Peter A Lieberzeit, Kiattawee Choowongkomon

{"title":"Synthesis and characterization of molecularly imprinted polymer nanoparticles against porcine circovirus type 2 viral-like particles.","authors":"Jutapak Klangprapan, Wisnu Arfian A Sudjarwo, Peter A Lieberzeit, Kiattawee Choowongkomon","doi":"10.1007/s00216-024-05576-3","DOIUrl":"10.1007/s00216-024-05576-3","url":null,"abstract":"PCV2 is a significant epidemic agricultural pathogen that causes a variety of swine diseases. PCV2 infections have significant economic impact on the swine industry, making effective strategies for rapid detection of PCV2 in pigs essential. Herein, we report on the synthesis of the so-called nano-MIPs which can be utilized for molecular recognition of PCV2. The morphology and structure of nano-MIPs were characterized using scanning electron microscopy (SEM). Nano-MIPs are spherical with sizes around 120-150 nm. Binding experiments demonstrate that the fluorescence intensity of PCV2 samples decreases proportionally to increasing the concentration of nano-MIPs due to quenching, while non-imprinted polymer nanoparticles (nano-NIPs) do not affect the signal. The Stern-Volmer constant of nano-MIPs binding to PCV2 was 1.3 × 10-3 mL/µg, whereas nano-NIPs led to 7 × 10-5 mL/µg, i.e., 1.8 orders of magnitude lower. The detection limit for binding MIP particles to PCV2 by fluorescence measurements is 47 µg/mL. This affinity test allows for designing both direct and competitive quartz crystal microbalance (QCM) assays for PCV2 leading to QCM measurements. The QCM results show nano-MIPs binding to PCV2 immobilized on the sensor surface with appreciable reproducibility. QCM sensor characteristics reveal signal saturation above around 200 µg/mL at a response of - 354 Hz and an LOD of approximately 35 µg/mL. Nano-MIPs also show selectivity factors of 2-5 for CSFV and PRRSV probably because the three viruses have similar diameters around 50 nm.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7357-7368"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an optimized SEC method for characterization of genome DNA leakage from adeno-associated virus products. 开发用于鉴定腺相关病毒产品基因组 DNA 泄漏的优化 SEC 方法。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-11-01 DOI: 10.1007/s00216-024-05623-z

Shuai Li, Xiaoyan Wang, Kuan-Yu Nick Lai, Jonathan Wert, Li Zhi, Mohammed Shameem, Dingjiang Liu

{"title":"Development of an optimized SEC method for characterization of genome DNA leakage from adeno-associated virus products.","authors":"Shuai Li, Xiaoyan Wang, Kuan-Yu Nick Lai, Jonathan Wert, Li Zhi, Mohammed Shameem, Dingjiang Liu","doi":"10.1007/s00216-024-05623-z","DOIUrl":"10.1007/s00216-024-05623-z","url":null,"abstract":"Adeno-associated virus (AAV) vectors are widely used to deliver therapeutic transgenes due to their superior safety, relatively low immunogenicity, and ability to target diverse tissues. AAV gene therapy products are typically formulated as frozen liquid and stored below - 60 °C, and therefore are subjected to multiple freeze/thaw cycles during manufacturing and administration. Recent studies have shown that genome DNA leakage could be induced by freeze/thaw stress. DNA leakage from AAV capsids has been reported to potentially impact product stability, induce immune responses, and compromise product efficacy. Thus, further characterization to improve the understanding of genome DNA leakage is necessary for mitigating the risks associated with genome DNA leakage during AAV product development. In this work, we developed an optimized size-exclusion chromatography (SEC) method for quantifying the leakage of genome DNA across multiple different AAV serotypes and demonstrated satisfactory assay performance in sensitivity, precision, and linearity. Furthermore, we showed that this method could also be applied to quantifying additional quality attributes of AAV, including the percentage of full capsids and quantification of AAV dimers. By using this optimized SEC method, we demonstrated that significantly increased free DNA was observed with increasing freeze/thaw cycles or at a temperature approaching the onset temperature for genome DNA ejection, which was effectively mitigated by the addition of 1.5% w/v sucrose in the AAV formulation. Thus, this optimized SEC method can serve as an invaluable tool for AAV formulation, product, and process development in ensuring the quality and stability of AAV gene therapy products.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7173-7182"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid identification of antibiotic residues in bovine kidney using coated blade spray-mass spectrometry. 使用涂层刀片喷雾质谱法快速鉴定牛肾中的抗生素残留。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-22 DOI: 10.1007/s00216-024-05605-1

Josha Jager, Sjors Rasker, Ane Arrizabalaga-Larrañaga, Rita Boerrigter-Eenling, Michel Rapallini, Marco Blokland

{"title":"Rapid identification of antibiotic residues in bovine kidney using coated blade spray-mass spectrometry.","authors":"Josha Jager, Sjors Rasker, Ane Arrizabalaga-Larrañaga, Rita Boerrigter-Eenling, Michel Rapallini, Marco Blokland","doi":"10.1007/s00216-024-05605-1","DOIUrl":"10.1007/s00216-024-05605-1","url":null,"abstract":"The use of certain antibiotics in food-producing animals is allowed in Europe following Regulation (EU) 2017/625. However, use could result in antibiotic residues in foodstuffs of animal origin. Maximum residue limits (MRLs) are in place to protect consumers. For monitoring purposes, animal matrices are tested to verify their compliance with these MRLs. Initially, matrices of (slaughtered) food animals are screened, often using a microbiological assay. Faster screening tests for antibiotics would be an advantage for control laboratories. Therefore, the present study describes, for the first time, the use of coated blade spray (CBS) followed by direct mass spectrometry (MS) analysis for the screening of tetracyclines, sulfonamides, quinolones, and macrolides residues from the renal area of intact bovine kidneys. An optimized workflow using two different desorption/ionization solutions per blade allowed screening of target compounds within 1 min per sample. The proof-of-principle of the CBS-MS method is validated according to (EU) 2021/808, presenting CCβ screening values of 0.1 × MRL for 43 analytes, 0.5 × MRL for 4 analytes, and 2.5 µg kg-1 for the prohibited substance dapsone, respectively. The developed method was successfully applied to seven official control samples of bovine kidneys. One of these samples was found to be positive using the CBS-MS method, which was confirmed as a true positive by LC-MSMS analysis. The developed method demonstrates that CBS devices can directly extract and analyze kidney samples for food safety testing.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7031-7041"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel ionic liquid-based approach for DNA and RNA extraction simplifies sample preparation for bacterial diagnostics. 基于离子液体的 DNA 和 RNA 提取新方法简化了细菌诊断的样品制备过程。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-11-08 DOI: 10.1007/s00216-024-05615-z

Johanna Kreuter, Katharina Bica-Schröder, Ádám M Pálvölgyi, Rudolf Krska, Regina Sommer, Andreas H Farnleitner, Claudia Kolm, Georg H Reischer

{"title":"A novel ionic liquid-based approach for DNA and RNA extraction simplifies sample preparation for bacterial diagnostics.","authors":"Johanna Kreuter, Katharina Bica-Schröder, Ádám M Pálvölgyi, Rudolf Krska, Regina Sommer, Andreas H Farnleitner, Claudia Kolm, Georg H Reischer","doi":"10.1007/s00216-024-05615-z","DOIUrl":"10.1007/s00216-024-05615-z","url":null,"abstract":"DNA- and RNA-based diagnostics play a pivotal role in accurately detecting and characterizing health-relevant bacteria, offering insights into bacterial presence, viability and treatment efficacy. Herein, we present the development of a novel extraction protocol for both DNA and RNA, designed to enable simple and rapid molecular diagnostics. The extraction method is based on the hydrophilic ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate and silica-coated magnetic beads. First, we developed an IL-based cell lysis protocol for bacteria that operates at room temperature. Subsequently, we established a magnetic bead purification procedure to efficiently and reproducibly extract DNA and RNA from the IL-lysates. The IL not only lyses the cells, but also facilitates the adsorption of nucleic acids (NAs) onto the surface of the magnetic beads, eliminating the need for a chaotropic binding buffer and allowing for purification of NAs without significant effort and materials required. Lastly, we combined the cell lysis step and the purification step and evaluated the novel IL-based extraction method on periopathogenic bacterial cultures, comparing it to commercial DNA and RNA extraction kits via (RT)-qPCR. In comparison to the reference methods, the IL-based extraction protocol yielded similar or superior results. Furthermore, costs are lower, required materials and equipment are minimal and the process is fast (30 min), simple and automatable. These characteristics favour the developed method for use in routine and high-throughput testing as well as in point-of-care, on-site and low-resource settings, thereby advancing the field of molecular diagnostics.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7109-7120"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid immunochemical methods for the analysis of proquinazid in strawberry QuEChERS extracts. 分析草莓 QuEChERS 提取物中丙奎那嗪的快速免疫化学方法。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-07-09 DOI: 10.1007/s00216-024-05428-0

José V Gimeno-Alcañiz, Francesc A Esteve-Turrillas, Antonio Abad-Fuentes, Consuelo Agulló, Ismael Navarro-Fuertes, Antonio Abad-Somovilla, Josep V Mercader

{"title":"Rapid immunochemical methods for the analysis of proquinazid in strawberry QuEChERS extracts.","authors":"José V Gimeno-Alcañiz, Francesc A Esteve-Turrillas, Antonio Abad-Fuentes, Consuelo Agulló, Ismael Navarro-Fuertes, Antonio Abad-Somovilla, Josep V Mercader","doi":"10.1007/s00216-024-05428-0","DOIUrl":"10.1007/s00216-024-05428-0","url":null,"abstract":"Proquinazid is a new-generation fungicide authorized in the EU for combating powdery mildew infections in high-value crops. Due to the perishable nature of fruits, alternative analytical methods are necessary to protect consumer's health from pesticide residues. Currently, immunoassays are a well-established approach for rapidly monitoring chemical contaminants. However, the production of high-quality immunoreagents, such as antibodies and bioconjugates, is essential. This study presents a newly designed hapten that maintains the characteristic moieties of proquinazid unmodified. The linear aliphatic substituents of this molecule were used to introduce the spacer arm. A three-step synthesis strategy was optimized to prepare a hapten that displays the entire 6-iodoquinazolin-4(3H)-one moiety with excellent yields. The N-hydroxysuccimidyl ester of the hapten was activated and purified to prepare a protein conjugate with high hapten density, which was used as an immunogen. Antibodies were raised and competitive enzyme-linked immunosorbent assays were developed. To enhance the assay's sensitivity, two additional heterologous haptens were prepared by modifying the halogenated substituent at C-6. The optimized assays demonstrated low limits of detection in buffer, approximately 0.05 μg/L. When applied to the analysis of proquinazid in QuEChERS extracts of strawberry samples, the immunoassays produced precise and accurate results, particularly in the 10-1000 μg/kg range.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7395-7404"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141557715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mass spectrometry database for the identification of marine animal saponin-related metabolites. 用于鉴定海洋动物皂素相关代谢物的质谱数据库。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-10 DOI: 10.1007/s00216-024-05586-1

Stuart J Smith, Scott F Cummins, Cherie A Motti, Tianfang Wang

{"title":"A mass spectrometry database for the identification of marine animal saponin-related metabolites.","authors":"Stuart J Smith, Scott F Cummins, Cherie A Motti, Tianfang Wang","doi":"10.1007/s00216-024-05586-1","DOIUrl":"10.1007/s00216-024-05586-1","url":null,"abstract":"Saponins encompass a diverse group of naturally occurring glycoside molecules exhibiting amphiphilic properties and a broad range of biological activities. There is a resurgence of interest in those saponins produced by marine organisms based on their potential therapeutic benefits, application in food products and most recently their potential involvement in intra- and inter-species chemical communication. The continual advancements in liquid chromatography techniques and mass spectrometry technologies have allowed for greater detection rates, as well as improved isolation and elucidation of saponins. These factors have significantly contributed to the expansion in the catalogue of known saponin structures isolated from marine invertebrates; however, there currently exists no specific chemical library resource to accelerate the discovery process. In this study, a Marine Animal Saponin Database (MASD v1.0) has been developed to serve as a valuable chemical repository for known marine saponin-related data, including chemical formula, molecular mass and biological origin of nearly 1000 secondary metabolites associated with saponins produced by marine invertebrates. We demonstrate its application with an exemplar asteroid extract (Acanthaster cf. solaris, also known as crown-of-thorns starfish; COTS), identifying saponins from the MASD v1.0 that have been previously reported from COTS, as well as 21 saponins isolated from multiple other related asteroid species. This database will help facilitate future research endeavours, aiding researchers in exploring the vast chemical diversity of saponins produced by marine organisms and providing ecological insights, and the realisation of their potential for various applications, including as pharmaceuticals.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6893-6907"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving signal-to-noise ratio of Raman measurements based on ensemble learning approach.

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-11-30 DOI: 10.1007/s00216-024-05676-0

Yufei Jia, Yuning Gao, Wenbin Xu, Yunxin Wang, Zejun Yan, Keren Chen, Shuo Chen

{"title":"Improving signal-to-noise ratio of Raman measurements based on ensemble learning approach.","authors":"Yufei Jia, Yuning Gao, Wenbin Xu, Yunxin Wang, Zejun Yan, Keren Chen, Shuo Chen","doi":"10.1007/s00216-024-05676-0","DOIUrl":"https://doi.org/10.1007/s00216-024-05676-0","url":null,"abstract":"Raman spectroscopy is an extensively explored vibrational spectroscopic technique to analyze the biochemical composition and molecular structure of samples, which is often assumed to be non-destructive when carefully using proper laser power and exposure time. However, the inherently weak Raman signal and concurrent fluorescence interference often lead to Raman measurements with a low signal-to-noise ratio (SNR), especially for biological samples. Great efforts have been made to develop experimental approaches and/or numerical algorithms to improve the SNR. In this study, we proposed an ensemble learning approach to recover and denoise Raman measurements with a low SNR. The proposed ensemble learning approach was evaluated on 986 pairs of Raman measurements, each pair of which consists of a low SNR Raman spectrum and a high SNR reference Raman spectrum from the exact same fungal sample but uses 200 times the integration time. Compared with conventional methods, the Raman measurements recovered by the proposed ensemble learning approach are more identical to high SNR reference Raman measurements, with an average RMSE and MAE of only 1.337 × 10-2 and 1.066 × 10-2, respectively; thus, the proposed ensemble learning approach is expected to be a powerful tool for numerically improving the SNR of Raman measurements and further benefits rapid Raman acquisition from biological samples.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An OPRM1-SNAP-tag/CMC method to directly identify drug components in sewage.

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-11-30 DOI: 10.1007/s00216-024-05672-4

Chenjia Li, Qi Liao, Rui Wang, Xinping Zhang, Mengyang Ma, Yonghong Liu, Lei Xiao, Ying Jiao, Nan Wang

{"title":"An OPRM1-SNAP-tag/CMC method to directly identify drug components in sewage.","authors":"Chenjia Li, Qi Liao, Rui Wang, Xinping Zhang, Mengyang Ma, Yonghong Liu, Lei Xiao, Ying Jiao, Nan Wang","doi":"10.1007/s00216-024-05672-4","DOIUrl":"https://doi.org/10.1007/s00216-024-05672-4","url":null,"abstract":"The scourge of drug addiction and abuse poses a significant challenge to society. Opioid drugs acting on μ-opioid receptor (OPRM1) make it one of the pivotal targets for drug addiction. In the past decade, sewage analysis has become a prevalent method of drug monitoring. However, traditional methods of detecting drugs in sewage are cumbersome, and rapid detection methods are relatively lacking. To address this, an innovative OPRM1-SNAP-tag/CMC method to directly identify drug components in sewage was established. Cell membrane chromatography (CMC) is an affinity chromatography technique which effectively detects receptor affinity substances. Cells constructed with high expression of specific receptor could be used to screen for compounds acting on the receptor. CMC based on OPRM1 provides a potentially convenient and effective tool for the detection of targeted drug components in sewage. In this study, the selectivity, reproducibility, column lifetime, and carryover of the CMC column had been assessed. Initially, we eluted the collected domestic sewage with methanol and acetonitrile, and the retention peaks were observed on the CMC system. Subsequently, without any preliminary sample preparation, we directly injected filtered samples of suspicious sewage into the OPRM1-SNAP-tag/CMC system, where we observed retention peaks as well. The retained components were further identified as morphine by using UPLC-MS/MS. In conclusion, the OPRM1-SNAP-tag/CMC method stands out as a reliable and robust model for the detection of drug components in sewage. It provides a valuable analytical tool for frontline drug control efforts, enhancing our capacity to monitor and mitigate the impact of drug abuse on society.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel BODIPY-derived colorimetric and ratiometric dual-mode fluorescent probe for highly sensitive and visual detection of sulfite in food and living organisms.

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-11-29 DOI: 10.1007/s00216-024-05658-2

Chunjie Zhang, Yueyin Liang, Shuai Gong, Zhiyuan Meng, Zhonglong Wang, Shifa Wang

{"title":"A novel BODIPY-derived colorimetric and ratiometric dual-mode fluorescent probe for highly sensitive and visual detection of sulfite in food and living organisms.","authors":"Chunjie Zhang, Yueyin Liang, Shuai Gong, Zhiyuan Meng, Zhonglong Wang, Shifa Wang","doi":"10.1007/s00216-024-05658-2","DOIUrl":"https://doi.org/10.1007/s00216-024-05658-2","url":null,"abstract":"Sulfite, widely used as a food additive, performs indispensable functions in the field of food sterilization, bleaching, and antisepsis. However, the overuse of sulfite may destroy food nutrition and pose health risks to people. In this work, an innovative BODIPY-based fluorescent probe (BODIPY-DBC) was constructed for highly sensitive recognition of sulfite. The BODIPY-DBC probe possessed both colorimetric and ratiometric dual mode, a low detection limit (33.12 nM), high sensitivity, a wide pH usage range (5-12), a fast response time (2 min), and superior fluorescence imaging capability for detecting sulfite. The recognition mechanism was certificated by 1H NMR titration, HRMS analysis, and DFT calculation. The BODIPY-DBC probe was not only loaded on test strips for detecting sulfite conveniently with the naked eye, but also employed to detect sulfite content in real food samples to ensure food safety. Furthermore, it also achieved excellent performances for monitoring sulfite in dual-channel fluorescence imaging (HeLa cell and zebrafish).","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging advances in biosensor technologies for quorum sensing signal molecules.

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-11-29 DOI: 10.1007/s00216-024-05659-1

Xi Chen, Chen Wang, Qing Yin Zheng, Wen-Chao Hu, Xing-Hua Xia

引用次数: 0