Analytical and Bioanalytical Chemistry最新文献

{"title":"Characterization of a PET reference material with exact-matching double isotope dilution MS and qNMR.","authors":"Andreas Breidbach","doi":"10.1007/s00216-026-06750-5","DOIUrl":"https://doi.org/10.1007/s00216-026-06750-5","url":null,"abstract":"<p><p>Polyethylene terephthalate (PET) microplastics are pervasive pollutants, yet reliable quantification is hampered by the scarcity of standardized protocols and lack of certified reference materials. To address this, the Joint Research Centre of the European Commission developed a liquid chromatography-exact-matching double isotope dilution mass spectrometry (LC-EMD-IDMS) method for characterizing a mass value of the PET reference material EURM-060. PET particles (< 300 µm) embedded in a NaCl carrier were hydrolyzed (110  <math><mmultiscripts><mrow></mrow> <mrow></mrow> <mo>∘</mo></mmultiscripts> </math> C, 120 min) to terephthalic acid (TPA). A perdeuterated TPA isotopologue (d <math><mmultiscripts><mrow></mrow> <mn>4</mn> <mrow></mrow></mmultiscripts> </math> -TPA) served as a spike, and calibration blends were prepared to meet the exact-matching criterion. MS acquisition in negative SRM mode provided isotope ratios, which, together with a rigorously derived measurement equation and a full uncertainty budget, yielded the PET mass in each unit. Ten randomly selected test units of EURM-060 gave masses between 451 and 586  <math><mi>μ</mi></math> g with relative standard uncertainties of 1.3-1.6 %. The average value is <math><msub><mi>X</mi> <mi>a</mi></msub> </math> =516  <math><mi>μ</mi></math> g±15  <math><mi>μ</mi></math> g (k=2), with a relative expanded uncertainty of 2.9 %. Independent verification by quantitative <math><mmultiscripts><mrow></mrow> <mrow></mrow> <mn>1</mn></mmultiscripts> </math> H-NMR on three additional test units (526  <math><mi>μ</mi></math> g±56  <math><mi>μ</mi></math> g) matched the LC-EMD-IDMS result within the expanded uncertainty range. The characterization meets the requirements of ISO 33405, providing a SI-traceable value of PET mass and a reference material that can reduce inter-laboratory variability in PET microplastic analysis.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of H₁R-SNAP-tag/CMC and screening for anti-allergic active ingredients targeting H₁R.","authors":"Quan Zhang, Yuhan Jiang, Wenhao Chen, Jinjin Wang, Xinchi Zhang, Yuan Wang, Zhuoyan Xu, Hengtao Dong, Shengli Han","doi":"10.1007/s00216-026-06799-2","DOIUrl":"https://doi.org/10.1007/s00216-026-06799-2","url":null,"abstract":"<p><p>In this study, a novel cell membrane chromatography (CMC) model was developed to discover Histamine H1 receptors (H₁R) targeted anti-allergic drugs for specific screening and ligand-receptor interaction analysis by SNAP-tagged H₁R fusion protein conjugation and BG-modified silica gel. Allergic diseases (ADs) are prevalent pathological conditions arising from immune dysregulation, which significantly compromise patient quality of life across multiple domains, while traditional Chinese medicines (TCMs) are widely used to treat ADs. H₁R plays a pivotal role in the pathogenesis of allergic diseases and acts as an attractive target for therapeutic intervention. Although antihistamines are the first-line therapy for ADs, their clinical utility is limited by side effects. Consequently, a novel screening method for active components targeting H₁R in TCMs is crucial for the research and discovery of novel anti-allergic lead compounds. In the current study, the H₁R-SNAP-tag cell membrane chromatography (H₁R-SNAP-tag/CMC) column was prepared with outstanding specificity, selectivity, and reproducibility by chemically immobilizing H₁R overexpression cell membranes to the BG-silica gel (BG-SiO₂) using the SNAP-tag technology. The H₁R-SNAP-tag/CMC-HPLC-MS system was investigated using the H₁R antagonist Clemastine fumarate and the results showed that the system was a reliable model for screening potential anti-allergic compounds from TCMs. From an Iris tectorum Maxim. (I. tectorum) extract, six H₁R-binding compounds were identified: tectoridin, iristectorin A, iridin, tectorigenin, irigenin, and irisflorentin. Frontal analysis and molecular docking confirmed their affinity for H₁R, while subsequent in vivo and in vitro pharmacological assays preliminarily validated their anti-allergic efficacy.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of the designer cathinone N-isopropylbutylone by a multi-technique approach and its metabolic profiling and stability assessment using dried matrix spots.","authors":"Sara Odoardi, Serena Mestria, Giulia Biosa, Valeria Valentini, Marta Bassi, Sabrine Bilel, Nicole Cocita, Matteo Marti, Giancarlo Fabrizi, Sabina Strano Rossi","doi":"10.1007/s00216-026-06793-8","DOIUrl":"https://doi.org/10.1007/s00216-026-06793-8","url":null,"abstract":"<p><p>Synthetic cathinones are one of the most prevalent new psychoactive substances on the drug market over the last 20 years. Their structural diversity, rapid turnover and variable stability in biological matrices pose significant challenges for laboratories. This study applied a comprehensive, multi-analytical workflow to characterise, profile the metabolism, and assess the stability of the synthetic cathinone N-isopropylbutylone, with a particular focus on dried matrix spot approaches. Structural elucidation was achieved through the combined use of GC-MS, LC-HRMS and NMR spectroscopy. This provided complementary information, enabling the compound to be identified with confidence. Investigating the metabolic profile in an animal model led to the identification of multiple phase I and phase II metabolites, including products of N-dealkylation, demethylation followed by O-methylation and glucuronidation. Differences in metabolite detection windows were noted between blood and urine. The applicability of dried blood spots (DBS) and dried urine spots (DUS) for metabolite detection and analyte stability was evaluated over a 90-day period. Dried matrix spots proved to be a valuable technique for analysing cathinones and their metabolites, and their enhanced stability in this medium stored at room temperature was confirmed, with most of the analytes being detectable throughout the entire study period. This study highlights the potential of dried matrix spot approaches as practical and effective tools for analysing synthetic cathinones and their metabolites. It also provides new analytical and metabolic data on N-isopropylbutylone. The proposed workflow could be used in future applications in forensic and clinical toxicology.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC-MS-based volatolomics and machine learning for predicting bloodstain time since deposition.","authors":"Wen-Ji Zhang, Qi-Rui Han, Ji-Long Zheng","doi":"10.1007/s00216-026-06783-w","DOIUrl":"https://doi.org/10.1007/s00216-026-06783-w","url":null,"abstract":"<p><p>Estimating the time since deposition (TSD) of bloodstains at crime scenes is critical in forensic science. Although various approaches have demonstrated feasibility and accuracy for TSD estimation, analysis of volatile organic compounds (VOCs) offers a promising alternative because it is noninvasive, rapid, and potentially cost-effective. In this study, human blood was chemically profiled using solid-phase microextraction coupled with gas chromatography-mass spectrometry (SPME-GC-MS) to investigate changes in VOC profiles over time and under different storage conditions. A total of 39 metabolites were associated with TSD. This study compared the types, abundances, and temporal patterns of VOCs among three substrates: gauze, wood, and glass. Based on 13 characteristic VOCs commonly detected in dried bloodstains across these three substrates within a 7-day period, a machine learning regression model was developed and evaluated. The optimal model was a random forest (RF) model, achieving root mean square error (RMSE), coefficient of determination (R²), and mean absolute error (MAE) values of 0.8217, 0.9401, and 0.5103, respectively, for the validation set, and 0.7742, 0.8533, and 0.5437, respectively, for the external validation set. These findings demonstrated that volatolomics analysis has strong potential for estimating bloodstain TSD, offering reliable biomarkers, high model accuracy, and robustness to substrate effects. This study provides a new methodological framework for TSD estimation and lays the foundation for further research in this field.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148904725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanded evaluation of a robust on-line sample cleanup-LC-MS platform suited for new approach methodologies (NAM).","authors":"Marcus Kamande, Julie Bekkhus, Stian Kogler, Aysan Safavi, Mads T S Gisle Johnsen, Patrik Appelblad, Steven Ray Wilson, Hanne Røberg-Larsen","doi":"10.1007/s00216-026-06775-w","DOIUrl":"https://doi.org/10.1007/s00216-026-06775-w","url":null,"abstract":"<p><p>Sample preparation in bioanalysis can require significant numbers of (manual) steps and consumables. Such procedures can be bottlenecks regarding cost, throughput, and greenness. This is also the case for analysis of new approach methodologies (NAMs) such as organoids and organ-on-a-chip systems. In this context, approaches tailored for measuring drugs in NAM-related cell culture media (CCM) are being developed. For mass spectrometry-based analysis, automated filtration/filter backflush solid-phase extraction liquid chromatography AFFL-SPE-LC is utilized. Case studies have shown that the \"AFFL\" platform allows for vast reductions in sample preparation efforts, as the \"self-cleaning\" filtration features and SPE conditions remove potentially clogging/contaminating materials from samples of biological origin, e.g., the salt and protein content in CCM. Here, broader demonstrations of the AFFL platform's traits for CCM analysis are provided, employing an extended panel of hydrophobic small-molecule drugs. The investigated AFFL platform shows reproducible chromatographic performance, as well as fit-for-purpose inter-matrix and inter-column robustness. Hundred-scale injections can be performed with satisfactory repeatability (e.g., retention time RSDs < 1%), with limited interference from the various CCM matrices investigated. Practical NAM applications are also demonstrated. The approach has clear advantages in greenness, obtaining an AGREE prep score of 0.71, contrasting the < 0.55 scores of more conventional/commercial approaches. The plastic consumable usage of our approach is 28 g/100 samples, a nearly 20-fold improvement over a previously established benchmark (500 g/100 samples). Taken together, these results demonstrate that AFFL provides a robust, low-waste, and scalable LC-MS-based workflow for chemical analysis of NAM-derived samples.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen-doped carbon nanodots-based \"off-on\" fluorescent sensor for the detection of breast cancer-derived exosomes and early diagnosis.","authors":"Hongchao Tang, Yiling Chen, Jiangfeng Shang, Liquan Zhu, Jingyuan Xu, Chaoshen Wu, Yibing Zhou, Wenqian Xu, Xiaozhen Liu, Da Qian, Xuli Meng, Chaoqi He","doi":"10.1007/s00216-026-06789-4","DOIUrl":"https://doi.org/10.1007/s00216-026-06789-4","url":null,"abstract":"<p><p>Breast cancer is a leading cause of cancer death in women, requiring non-invasive early diagnostic tools. Exosomes are promising liquid biopsy biomarkers that reflect tumor-derived molecular information. Here, we developed an \"off-on\" fluorescent sensor using nitrogen-doped carbon nanodots (N-CNDs) for sensitive detection of breast cancer-derived exosomes. The sensor uses a magnetic bead-based aptamer complex (MBs-Apt-P1-P2) for target capture. P1 and P2 probes contain both EpCAM-recognition and Poly-T sequences. In the absence of targets, Ag⁺ quenches N-CNDs fluorescence. Upon target introduction, aptamers bind exosomal EpCAM, competitively releasing P1/P2 probes. After magnetic separation, the released Poly-T sequences capture Ag⁺ via T-Ag⁺-T coordination, restoring N-CNDs fluorescence. Each exosome can release two probes, providing dual signal amplification. Under optimal conditions, the sensor responded linearly to 1 × 10⁹-4 × 10¹¹ particles/mL with a detection limit of 35 particles/mL, showing excellent sensitivity for EpCAM-positive breast cancer exosomes. Clinical testing on 11 serum samples (6 patients, 5 healthy donors) distinguished patients with an AUC of 0.94, compared with ELISA (AUC = 0.88). This sensor offers a promising platform for breast cancer detection with high sensitivity and accuracy, warranting further validation in large-scale clinical studies.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative method comparison in non-targeted analysis using cascade classification.","authors":"Nimesh P R Ranasinghe Arachchige, Kanti Sapkota, Omar Rivera, Krishna Patel, H Jamie Kim, Milani Patabandige, Thilini Perera, Joshua A Young","doi":"10.1007/s00216-026-06787-6","DOIUrl":"10.1007/s00216-026-06787-6","url":null,"abstract":"<p><p>Non-targeted analysis (NTA) lacks consistent processes for comparing method performance across different analytical conditions. As a result, method comparison between NTA methods is often dependent on the chemicals detected in a particular study, making it difficult to objectively evaluate how instrumental performance influences the overall detectable space. Here, we describe a two-stage cascade classification approach that treats detectability as a binary outcome defined by experimental retention behavior and signal-to-noise criteria and characterizes the boundary between detectable and non-detectable chemical space using receiver operating characteristic (ROC) analysis. In Stage 1, compounds are filtered by estimated retention index to establish chromatographic accessibility. In Stage 2, detectability is classified using the electron impact cross-section (Q), with the classification threshold selected to satisfy a minimum recall constraint (recall ≥ 0.80). Using this treatment for the detectable space, the area under the ROC curve (AUC) emerges as a method-level figure of merit. Applied to two GC-MS systems operating under identical chromatographic conditions but with different mass selective detectors (Agilent 6890/5975B and 8890/5977C), the approach yielded Stage 2 AUC values of 0.659 (95% CI: 0.536-0.788) for Method A and 0.842 (95% CI: 0.702-0.933) for Method B at 10 μg/mL, quantitatively capturing the performance difference between instruments. The minimum-recall criterion selected near-identical classification thresholds for both methods (τ = 19.18 and 19.30, respectively), indicating that Q estimates essentially the same detectable chemical space for both instruments, and that the observed difference in detectable space reflects instrument performance. This approach provides a reproducible and transparent basis for GC-MS method comparison in NTA and refines the statistical basis needed for a generalized analytical evaluation of NTA methods.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148904821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antigen-binding-enhanced divalent nanobody-based immunomagnetic separation coupled with ATP bioluminescence for viable Salmonella detection.","authors":"Yu Ma, Weiying Kong, Jiale Xu, Haibo Wang, Zimei Mo, Jia Wang","doi":"10.1007/s00216-026-06808-4","DOIUrl":"https://doi.org/10.1007/s00216-026-06808-4","url":null,"abstract":"<p><p>Current Salmonella detection methods are time-consuming or incapable of effectively discriminating viable bacteria from dead cells, restricting their practical applications in food safety monitoring. Thus, in this study, an antigen-binding-enhanced Salmonella divalent nanobody (diVHH) was optimally expressed and chemically conjugated with magnetic beads (MBs). Combined with ATP bioluminescence, a novel strategy was developed for the rapid detection of viable Salmonella. Optimization of prokaryotic expression significantly improved the diVHH yield, with an approximately 2.96-fold enhancement. Then, the diVHH-functionalized MBs achieved capture efficiencies exceeding 90% for multiple tested Salmonella serotypes. By integrating immunomagnetic separation with ATP bioluminescence, the established assay exhibited a linear detection range from 10² to 10⁶ CFU/mL, with a limit of detection of 10 CFU/mL for S. Enteritidis and 12 CFU/mL for S. Typhimurium. The entire detection procedure could be completed within 2 h. In spiked food samples, the method achieved favorable recovery rates of 96.00-106.89% with low coefficients of variation (0.78-7.54%). Notably, this method could be successfully applied to the semi-quantitative analysis of multiple Salmonella serotypes in environmental and food samples, and the obtained results were highly consistent with plate counting results. This study demonstrates that engineered diVHHs exhibit enhanced antigen‑binding capacity and can serve as excellent recognition reagents for selective Salmonella detection to enable a reliable and rapid assay for viable Salmonella with promising application potential.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intercalation chemistry activates Co(OH)₂ for high-performance non-enzymatic glucose sensing via synergistic phase and microenvironment engineering.","authors":"Tao Li, Luyao Wang, Ziyin Yang","doi":"10.1007/s00216-026-06796-5","DOIUrl":"https://doi.org/10.1007/s00216-026-06796-5","url":null,"abstract":"<p><p>The electrochemical inertness of conventional β-Co(OH)₂ limits its application in non-enzymatic glucose sensing. Here, we demonstrate that MoO₄^2-intercalation substantially activates Co(OH)₂ by simultaneously triggering a crystallographic phase transformation from β to α phase and modulating the local electronic microenvironment. The intercalated anions expand the interlayer galleries, yielding a 1.7-fold enlargement of the electrochemically active surface area, withdraw electrons from Co centers to facilitate the generation of high-valent Co(Ⅲ)/Co(Ⅳ) active species at lowered potentials, and markedly strengthen glucose adsorption. Consequently, the MoO₄^2--intercalated Co(OH)₂ achieves an ultrahigh sensitivity of 2743.9 µA mM^-1 cm^-2, which is 7.3 times that of pristine Co(OH)₂, together with a wide linear range of 4.0 µM to 0.2 mM and a low detection limit of 0.36 µM. This work establishes an intercalation-driven dual-regulation strategy that integrates phase engineering and microenvironment modulation, offering a generalizable approach for the design of advanced sensing materials.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-sensitive determination of midazolam in urine samples using hydrogen peroxide-free enhanced electrochemiluminescence sensor based on modified polypyrrole/carbon paste electrode.","authors":"Farzane Ahmadi, Naader Alizadeh","doi":"10.1007/s00216-026-06791-w","DOIUrl":"https://doi.org/10.1007/s00216-026-06791-w","url":null,"abstract":"<p><p>This paper proposes an enhanced electrochemiluminescence (ECL) nanostructure sensor for midazolam (Mdz) determination via one-step electropolymerization of the conductive molecularly imprinted polymer (CMIP) based on polypyrrole (PPy) on a carbon paste electrode (CPE) by the cyclic voltammetric (CV) technique. The FESEM technique showed that the surface of the sensor is made of nano-sized particles. In the absence of hydrogen peroxide, ECL signals of luminol were investigated and an enhanced response was observed by adding Mdz to the solution. Optimal experimental conditions were obtained for the proposed sensor. Its ECL behavior was investigated and the reaction mechanism was discussed. The calibration curve of the CMIP sensor was obtained in the dynamic linear range from 10 to 400 nM with a limit of detection (LOD) of 6 nM for the Mdz determination in solution. The good selectivity of the proposed CMIP sensor was shown by investigating the effect of some interferences on the CMIP and non-imprinted polymer electrode. This sensor was able to determine the amount of Mdz in urine samples with acceptable sensitivity.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}