Analytical and Bioanalytical Chemistry最新文献

{"title":"Flow cytometry for rapid analysis of bacteriostatic versus bactericidal effects in Legionella pneumophila disinfection.","authors":"Yiao Liang, Lena Heining, Martin Elsner, Michael Seidel","doi":"10.1007/s00216-025-06055-z","DOIUrl":"https://doi.org/10.1007/s00216-025-06055-z","url":null,"abstract":"<p><p>Biocides, encompassing both oxidizing agents such as chlorine and non-oxidizing agents like quaternary ammonium compounds, are extensively employed to minimize the risk of Legionella pneumophila (L. pneumophila) outbreaks in evaporative cooling systems. Currently, biocide effectiveness to combat L. pneumophila is determined by cultivation test methods, which have the disadvantage of long testing times and fail to detect a viable but non-culturable status of bacteria. Cultivation-independent bacterial counting methods would, therefore, be superior for future analysis of biocide effects. Herein, we employed total cell counting and intact cell counting by flow cytometry (FCM) as rapid, cultivation-independent, and untargeted methods for effect-based analysis of biocide effectiveness. We used total cell counting and intact cell counting to detect DNA damage and membrane integrity changes in response to oxidizing sodium hypochlorite (NaClO) and non-oxidizing benzalkonium chloride (BAC). Biocide effect analysis over time was conducted on a dedicated cartridge-based flow cytometer for bacteria analysis, rqmicro.COUNT. The effects of NaClO and BAC on cell membranes and DNA could be well determined and distinguished. Process water contaminated by L. pneumophila was treated with different concentrations of NaClO and BAC, followed by regrowth experiments to determine bacteriostatic versus bactericidal effects. The results showed that a considerable reduction in intact cell count was related to the bacteriostatic effects of the treatment and the success of the disinfection of L. pneumophila according to the standard cultivation method. A significant decrease in total cell count indicating DNA damage was associated with bactericidal effects. Hence, we could show the potential of total cell counting and intact cell counting by FCM to be used as a rapid and effect-based analysis method for minimizing both health-related risks and biocide usage in process water.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell Raman and mass spectrometry analysis to probe cellular heterogeneity in tamoxifen uptake and metabolism","authors":"Congrou Zhang,&nbsp;Yasmine Abouleila,&nbsp;Sylvia Le Dévédec,&nbsp;Thomas Hankemeier,&nbsp;Arno Germond,&nbsp;Ahmed Ali","doi":"10.1007/s00216-025-06058-w","DOIUrl":"10.1007/s00216-025-06058-w","url":null,"abstract":"<div><p>Current drug discovery is limited by the lack of single-cell data on drug uptake, metabolism, and effects, as population-level methods obscure cellular heterogeneity. While single-cell RNA sequencing has revealed drug resistance mechanisms, it cannot simultaneously measure drug concentrations and cellular responses. Raman spectroscopy probes single-cell drug effects but lacks sensitivity for drug or its metabolite quantification, whereas single-cell mass spectrometry (MS) offers high sensitivity but consumes samples, preventing repeated measurements. Integrating Raman spectroscopy with MS enables simultaneous assessment of cellular states and drug metabolism. However, existing studies are limited by small sample sizes and single drug concentrations. We employ a combined single-cell Raman and mass spectrometry (Raman-MS) approach to investigate variability in drug uptake, metabolism, and effects in HepG2 liver cancer cells. The cells were exposed to three concentrations of tamoxifen, after which we quantified the heterogeneity in tamoxifen and its hepatotoxic metabolites. This validates the potential of single-cell analysis for advancing drug discovery and cancer research. Our results indicated that tamoxifen induces concentration-dependent metabolic changes in single liver cancer cells, as revealed by Raman spectroscopy and mass spectrometry. The findings highlight a potential threshold concentration beyond which cellular integrity is compromised, underscoring the importance of single-cell approaches for understanding drug uptake, metabolism, and therapeutic heterogeneity.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5349 - 5358"},"PeriodicalIF":3.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12431888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raman imaging of molecular groups in the wavenumber silent region.","authors":"Constanze Schultz, Jürgen Popp","doi":"10.1007/s00216-025-06029-1","DOIUrl":"https://doi.org/10.1007/s00216-025-06029-1","url":null,"abstract":"<p><p>Raman imaging in the wavenumber silent region emerged around 15 years ago as a powerful tool for visualizing biomolecules and synthetic compounds in complex environments with minimal spectral and biological interference. Since then, the field has advanced from simple proof-of-concept studies using available tags to the rational design of highly efficient Raman labels with sharp silent region signatures, now applied to real biological and biomedical questions. This review traces the evolution from the versatility of label-free Raman to the increasing relevance of labeled strategies, emphasizing how tag design influences functionality, application, and impact. We highlight recent progress in both the synthesis and deployment of tags tailored for specific cellular targets and processes and discuss the emerging need for labeled strategies to meet the demands of sensitivity, multiplexing, and biocompatibility in complex systems. Through this design-to-application perspective, the review provides a comprehensive overview of the current capabilities and significant applications, and identifies key future directions to fully exploit the potential of silent region Raman imaging.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface plasmon resonance sensor for direct oxytetracycline detection with a newly selected aptamer","authors":"Meiting Kang,&nbsp;Liqing Wu,&nbsp;Qiang Zhao","doi":"10.1007/s00216-025-06052-2","DOIUrl":"10.1007/s00216-025-06052-2","url":null,"abstract":"<div><p>The overuse of tetracycline antibiotics can lead to its residue in the aquatic ecological environment and subsequently cause risks to human health through the food chain. Therefore, effective determination of oxytetracycline (OTC) residues is urgently needed. In this study, a simple and direct aptamer-based surface plasmon resonance (SPR) sensor has been fabricated and applied for selective and sensitive detection of the antibiotic OTC by using the newly reported short aptamer for OTC. Biotinylated aptamers were immobilized on the surface of a SPR chip functionalized with streptavidin. When OTC bound to its aptamer, the refractive index of the SPR chip changed, and the SPR signal response increased. The SPR chip could be regenerated by simply flowing the running buffer without the need for an additional regeneration solution. We found that a high density of aptamer immobilized on the SPR chip allowed for an enhanced SPR response to OTC. This aptamer SPR sensor had a wide dynamic range of 1.56 nM to 3.2 µM for OTC detection with a detection limit of 3.6 nM. This SPR sensor can be reused and shows high stability. This method demonstrated good specificity towards OTC and enabled us to detect OTC in diluted lake water, showing applicability for OTC detection in practical applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5315 - 5322"},"PeriodicalIF":3.8,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biopharmaceutical analysis - current analytical challenges, limitations, and perspectives.","authors":"Diana R Cunha, M Beatriz Quinaz, Marcela A Segundo","doi":"10.1007/s00216-025-06036-2","DOIUrl":"https://doi.org/10.1007/s00216-025-06036-2","url":null,"abstract":"<p><p>Biopharmaceutical analysis is essential for ensuring the quality, safety, and efficacy of these medicines. Nonetheless, it faces significant challenges due to the complexity of these molecules and the strict regulatory standards. This work explores the strengths, limitations, opportunities, and threats in biopharmaceutical analysis, focusing on the challenges and emerging analytical technologies and their impact on biopharmaceutical development and quality control. Key challenges identified include the complexity and heterogeneity of biopharmaceuticals leading to the requirement for a broad spectrum of analytical methods, the high cost of the required analytical instrumentation, and the need for skilled professionals to implement advanced analytical techniques. Integrating artificial intelligence-driven data analysis, automation, and multi-omics approaches can significantly improve analytical precision and efficiency. However, regulatory barriers and financial constraints remain critical obstacles to widespread adoption. This work highlights the need for industry-wide collaboration to standardize methodologies and regulations, improve data integrity, and facilitate regulatory acceptance by reviewing the last 5 years of biopharmaceutical-related works. Ultimately, the continued development of biopharmaceutical analysis, driven by technological advancements and strategic investments, is crucial for biopharmaceutical development, enhancing manufacturing efficiency, and ensuring broader access to high-quality biopharmaceuticals.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic quality control of Shi Jue Ming San by two-dimensional liquid chromatography/mass spectrometry and adjusted multiple reaction monitoring of quality markers","authors":"Wenfan Nie,&nbsp;Ying Wang,&nbsp;Jinhui Du,&nbsp;Wei Mi,&nbsp;Kexin Qi,&nbsp;Zhonglian Zhang,&nbsp;Zhifei Fu,&nbsp;Lifeng Han","doi":"10.1007/s00216-025-06041-5","DOIUrl":"10.1007/s00216-025-06041-5","url":null,"abstract":"<div><p>Shi Jue Ming San (SJMS) is a traditional Chinese medicine formula, which is primarily used in clinical practice for treating hypertension and eye diseases. However, due to the complex composition of SJMS, studies on its quality control and chemical constituents remain limited. This study aims to establish a systematic quality control method on SJMS through qualitative and quantitative analyses using chromatography-mass spectrometry. Firstly, an offline two-dimensional liquid chromatography/quadrupole-Orbitrap-MS method (offline 2D-LC/Q-Orbitrap-MS) was established for multi-component characterization. The offline 2D-LC system, consisting of an XAmide column and an HSS T₃ column, exhibited an orthogonality of 0.61 and a theoretical peak capacity of 7038. As a result, a total of 153 compounds were identified. Subsequently, an online two-dimensional liquid chromatography/quadrupole-time-of-flight MS (online 2D-LC/Q-TOF–MS) method was developed. The online system, integrating a Poroshell 120 HILIC-Z column (first dimension) and a Poroshell 120 Aq-C18 column (second dimension), exhibited an orthogonality of 0.58 and a better peak capacity of 8675. The component screening capability was preliminarily evaluated and compared with the offline 2D-LC–MS method. A total of 177 compounds were detected, and after removing duplicates identified in the offline system, 263 compounds were ultimately characterized. Finally, a co-condition fingerprint analysis for four single herbs of SJMS was performed, identifying seven bioactive compounds (chlorogenic acid, liquiritin, cynaroside, nodakenin, linarin, glycyrrhizic acid, and aurantio-obtusin) as quality markers of SJMS. An adjusted multiple reaction monitoring (MRM^ad) method based on UPLC-QQQ-MS was established for the quantitative analysis of these markers in 15 batches of SJMS. This study represents comprehensive multi-component characterization, identification, and quality control of SJMS for the first time, providing a scientific basis for its quality control.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5221 - 5237"},"PeriodicalIF":3.8,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multiple-cross self-powered sensor based on photo-assisted zinc-air battery for spatio-temporal reconciliated accurate assay","authors":"Xiaojiao Du,&nbsp;Yanguang Yang,&nbsp;Ding Jiang,&nbsp;Xueling Shan,&nbsp;Wenchang Wang,&nbsp;Hiroshi Shiigi,&nbsp;Zhidong Chen","doi":"10.1007/s00216-025-06047-z","DOIUrl":"10.1007/s00216-025-06047-z","url":null,"abstract":"<div><p>The development of a spatio-temporal coordination and multiple-cross detection platform is crucial for the highly accurate assay in complex sample or varied environments. In this study, a sensitive and accurate dual-mode self-powered electrochemical sensor (SPES) was constructed based on the Z-scheme heterojunction-promoted photo-assisted zinc-air battery (ZAB) and electrochromic (EC) technology for the multiple-cross quantitative analysis of gallic acid (GA). With AgBr/BiFeO₃ Z-scheme heterojunction as the photocathode, the photo-assisted ZAB functions as an energy collection and conversion device to realize the SPES with enhanced energy conversion efficiency. Furthermore, this platform enables the sensitive and selective detection of GA with the use of molecular imprinting technology. In detail, the presence of GA initiates its binding to the molecularly imprinted cavity, subsequently hindering electron transfer on the electrode surface. This not only reduces output power density but also hinders electron participation in the electrochromic reaction, leading to a color change. By combining the advantages of SPES and EC technology, this platform enables simultaneous collection and multiple cross-validation of electrochemical signals and visual signals, thereby enhancing detection accuracy. The limit of detection of SPES and EC was found to be 1.2 × 10^–10 M and 1.9 × 10^–10 M (S/N = 3), respectively. This research offers a new idea for the construction of a highly accurate dual-mode sensing platform with multiple-cross signals and convenient operation.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5275 - 5285"},"PeriodicalIF":3.8,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00216-025-06047-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploiting metal-free atom transfer radical polymerization for fabrication of styrene-maleic acid polymer-brush grafting chromatographic stationary phase and its separation performances for phospholipids","authors":"Ruilin Yang,&nbsp;Ping Wang,&nbsp;Xinhui Guan,&nbsp;Xiaofei Gu,&nbsp;Xiaoqiang Qiao","doi":"10.1007/s00216-025-06053-1","DOIUrl":"10.1007/s00216-025-06053-1","url":null,"abstract":"<div><p>Phospholipids have been considered biomarkers for diagnosing many diseases in recent years. However, the isolation and analysis of phospholipids still face many difficulties. Styrene-maleic acid (SMA) copolymer is currently considered suitable for the separation and analysis of phospholipids due to its ability to enhance and improve the solubility of phospholipid bilayers on cell membranes. In this work, metal-free atom transfer radical polymerization was first exploited to graft SMA copolymer onto the surface of silica gel. With glycerol monolaurate (GML) as the derivatization reagent, Sil-SMA-GML polymer-brush silica-based stationary phase was first developed. The chromatographic retention mechanism revealed that the Sil-SMA-GML column is of hydrophilic/reversed-phase mixed-mode retention modes. The chromatographic separation performance evaluation indicated that the Sil-SMA-GML column had excellent separation capabilities for both hydrophilic and hydrophobic compounds. The maximum column efficiency was up to 78,600 N/m. The Sil-SMA-GML column could also achieve simultaneous separation and analysis of different phospholipid classes and species as well as complex phospholipid extracts from human serum and exosomal phospholipid extracts, demonstrating the good potential of the developed stationary phase.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5323 - 5333"},"PeriodicalIF":3.8,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fluorescent biosensor based on glucose oxidase-DNAzyme/substrate complex synergy for salivary glucose monitoring","authors":"Longping Yang,&nbsp;Huiyu Tian,&nbsp;Chengcheng Liu,&nbsp;Fangning Jiang,&nbsp;Yan Dong,&nbsp;Yongjie Sheng,&nbsp;Yanhong Sun,&nbsp;Yanqun Fei,&nbsp;Jiacui Xu,&nbsp;Dazhi Jiang","doi":"10.1007/s00216-025-06051-3","DOIUrl":"10.1007/s00216-025-06051-3","url":null,"abstract":"<div><p>Non-invasive glucose sensing technology exhibits significant clinical potential for continuous diabetes monitoring and early screening in high-risk populations. Herein, we developed a modular fluorescent biosensor for salivary glucose monitoring through synergistic integration of a detection module (glucose oxidase) and a signal transduction module (PL DNAzyme-substrate complex). The sensing system employs a fluorophore/quencher pair-modified PL DNAzyme-substrate complex as a fluorescence switching element. Experimental results showed a linear detection range from 40 nM to 1.0 mM with a detection limit of 4.3 nM, demonstrating a 1163-fold improvement in sensitivity compared with the previous version. The biosensor exhibited excellent specificity and stability. It was used to detect 2-h postprandial saliva samples from 15 participants (mean glucose concentration: 53.7 ± 19.5 μM), and its extended functionality was demonstrated through continuous daily monitoring of three participants and 14-day consecutive tracking tests, which successfully captured temporal glucose fluctuations. This work presents a fluorescence-based platform for non-invasive salivary glucose monitoring, while its modular architecture provides a versatile framework for future performance optimization through component substitution.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 23","pages":"5303 - 5313"},"PeriodicalIF":3.8,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging open cheminformatics tools for non-targeted metabolomics analysis of C. elegans: a workflow comparison and application to strains related to xenobiotic metabolism and neurodegeneration.","authors":"Gianfranco Frigerio, Yunjia Lai, Emma L Schymanski, Gary W Miller","doi":"10.1007/s00216-025-06048-y","DOIUrl":"https://doi.org/10.1007/s00216-025-06048-y","url":null,"abstract":"<p><p>Caenorhabditis elegans (C. elegans) is a well-established nematode model for studying metabolism and neurodegenerative disorders, such as Alzheimer's (AD) and Parkinson's disease (PD). Non-targeted metabolomics via liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has proven useful for uncovering metabolic changes in biological systems. Here, we present workflows for C. elegans metabolomics, leveraging advanced open science tools. We compared two metabolite extraction methods: a monophasic extraction, which provided broader metabolite coverage in analyses conducted in hydrophilic interaction with positive polarity (HILIC POS), and a biphasic extraction, which yielded more features in reverse-phase C18 chromatography with negative polarity (RPLC NEG) analyses. Data were processed using patRoon, integrating IPO, XCMS, CAMERA, and MetFrag, which incorporated PubChemLite compounds and C. elegans-specific metabolites from an expanded WormJam database enhanced with PubChem and literature sources. MS-DIAL was also employed for data processing, allowing for expanded annotations with predicted spectra for the expanded WormJam metabolites calculated using CFM-ID. Significant metabolite differences were identified when comparing the Bristol (N2) wild-type strain with two knockout strains of xenobiotic-metabolizing enzymes and two transgenic strains related to neurodegenerative pathways. Pooled quality control (QC) samples for each strain ensured robust data quality and the detection of strain-related metabolites. Our study demonstrates the potential of non-targeted metabolomics for metabolite discovery employing open science tools in model organisms.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}