Analytical and Bioanalytical Chemistry最新文献_第8页

miRNA203 detection via single-strand DNA hybridization and signal amplification by copper-doped cerium oxide nanoparticles. 单链DNA杂交和铜掺杂氧化铈纳米颗粒信号放大检测miRNA203。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-07-01 Epub Date: 2025-05-30 DOI: 10.1007/s00216-025-05926-9

Chaimae El Ghzaoui, Craig J Neal, Bijay Dhungana, Shanza Nusrat, Yifei Fu, Elayaraja Kolanthai, Sudipta Seal

{"title":"miRNA203 detection via single-strand DNA hybridization and signal amplification by copper-doped cerium oxide nanoparticles.","authors":"Chaimae El Ghzaoui, Craig J Neal, Bijay Dhungana, Shanza Nusrat, Yifei Fu, Elayaraja Kolanthai, Sudipta Seal","doi":"10.1007/s00216-025-05926-9","DOIUrl":"10.1007/s00216-025-05926-9","url":null,"abstract":"Diabetes mellitus is a debilitating disease that can result in the formation of foot ulcers. Healing of these ulcers is additionally impeded, as a consequence of the condition, leading to the persistence of the wounds which can lead to additional ulceration and infection. miRNA203 expression has been correlated with the severity of diabetic wounds and can therefore function as a biomarker for wound health. In the presented study, an electrochemical platform sensor device was produced which allowed the detection of the miRNA203 analyte at concentrations from 1 μM down to 10 fM. The sensor was built using an electrode-bound single-strand DNA species, designed to promote selective hybridization to miRNA203, which was chemically functionalized to a redox-active copper-doped cerium oxide (CuCNP) particle formulation, mediating charge transfer to the electrode substrate. CuCNP synthesis was performed at varying copper contents (0, 5, 8, and 12 mol%) and each formulation was tested to identify an optimal composition for charge transfer in the sensor architecture.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"4083-4095"},"PeriodicalIF":3.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep learning-based analysis and identification of single-particle mass spectra of bacteria. 基于深度学习的细菌单粒子质谱分析与鉴定。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-07-01 Epub Date: 2025-06-21 DOI: 10.1007/s00216-025-05942-9

Hong Chen, Ning Zhang, Yao-Hua Du, Xiao-Bo Zhan, Lei Li, Zhi Cheng

{"title":"Deep learning-based analysis and identification of single-particle mass spectra of bacteria.","authors":"Hong Chen, Ning Zhang, Yao-Hua Du, Xiao-Bo Zhan, Lei Li, Zhi Cheng","doi":"10.1007/s00216-025-05942-9","DOIUrl":"10.1007/s00216-025-05942-9","url":null,"abstract":"Single-particle mass spectrometry (SPMS) has the potential to identify bacterial species. However, this crucial topic has received limited attention in research. This investigation aims to fill this gap by combining SPMS with supervised learning algorithms to distinguish six bacterial species. The study begins by collecting particle size and mass spectra data for six bacteria and four biomass combustion products (BCPs) using SPMS. These data are used to compare particle sizes and create a comprehensive dataset containing mass spectra for all ten subjects. The mass spectra peak ratio method is then employed to differentiate between bacteria and BCPs, highlighting their distinct distributions of PO₃-/PO₂- and CNO-/CN- in scatter plots. In addition to this, the study compares the mass spectrometry ion features of bacteria and BCPs and evaluates the classification performance of support vector machines (SVM), multi-layer perceptrons (MLP), and convolutional neural networks (CNN) using five criteria. The Score-Weighted Class Activation Mapping (Score-CAM) method is used to visualize and analyze the CNN models, extracting and analyzing the key ionic features that the CNN models relied on for classification. The results demonstrate that the mass spectra peak ratio method effectively distinguishes bacteria from BCPs. The CNN and MLP algorithms can not only accurately distinguish between bacteria and BCPs but also precisely identify different types of bacteria. The overall classification accuracy of the CNN and MLP models exceeds 96%. The key ions obtained using the Score-CAM method exhibit varying degrees of signal intensity differences among different bacteria, which helps to understand the compositional differences between various bacterial species. This study provides an effective methodology for the in-depth analysis of SPMS data.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"4265-4277"},"PeriodicalIF":3.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous determination of hydroquinone, catechol, and resorcinol with an electrochemical sensor based on poly-L-valine, multi-walled carbon nanotubes, and Co₃O₄ nanoparticles. 基于聚l -缬氨酸、多壁碳纳米管和纳米Co3O4的电化学传感器同时测定对苯二酚、儿茶酚和间苯二酚。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-07-01 Epub Date: 2025-05-26 DOI: 10.1007/s00216-025-05923-y

Noor Raad Abdulrasool, İrem Okman Koçoğlu

{"title":"Simultaneous determination of hydroquinone, catechol, and resorcinol with an electrochemical sensor based on poly-L-valine, multi-walled carbon nanotubes, and Co3O4 nanoparticles.","authors":"Noor Raad Abdulrasool, İrem Okman Koçoğlu","doi":"10.1007/s00216-025-05923-y","DOIUrl":"10.1007/s00216-025-05923-y","url":null,"abstract":"An electrochemical sensor based on carboxylated multi-walled carbon nanotubes (c-MWCNT), Co3O4 nanoparticles (Co3O4NP), and poly-L-valine (PolyVal) was fabricated for the simultaneous determination of hydroquinone (Hyd), catechol (Cat), and resorcinol (Res). The optimum amounts of modification materials on the electrode surface and the optimum pH of the working solution were determined to achieve the best electrode performance. Under optimum conditions, individual determination of each analyte was performed in the presence of other analytes. Simultaneous determination of Hyd, Cat, and Res was also carried out, and the performance characteristics of the electrode, such as working range, LOD, reusability, reproducibility, and selectivity, were evaluated for each analyte. In the simultaneous determination for Hyd, Cat, and Res, the linear working ranges were 5-260 µM, 5-280 µM, and 31-550 µM, and the LOD values were 3.62 µM, 2.57 µM, and 7.97 µM, respectively. The presented electrochemical sensor was successfully applied to the simultaneous determination of Hyd, Cat, and Res in tap water and river water, and satisfactory recovery values were obtained.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"4053-4067"},"PeriodicalIF":3.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of polymer monolith-MOF hybrid via surface functionalization for bioanalytical sciences. 基于表面功能化的聚合物单体- mof杂化物在生物分析科学中的应用。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-07-01 Epub Date: 2025-03-12 DOI: 10.1007/s00216-025-05822-2

Kinza Ali, Iqra Munawar, Sara Manan, Fatima Nawazish, Batool Fatima, Fahmida Jabeen, Adeela Saeed

{"title":"Development of polymer monolith-MOF hybrid via surface functionalization for bioanalytical sciences.","authors":"Kinza Ali, Iqra Munawar, Sara Manan, Fatima Nawazish, Batool Fatima, Fahmida Jabeen, Adeela Saeed","doi":"10.1007/s00216-025-05822-2","DOIUrl":"10.1007/s00216-025-05822-2","url":null,"abstract":"Monoliths are versatile materials with diverse applications, and their performance can be enhanced through modifications, including the use of metal-organic frameworks (MOFs). Modified monoliths improve separation and analytical processes in various fields, with different modification methods offering distinct benefits and challenges. Directly adding MOF crystals to the polymerization mixture is straightforward and time effective, but it often results in poor dispersion and compositional heterogeneity, which compromises consistency and reproducibility, particularly in bioanalytical applications. Although layer-by-layer (LbL) development or post-synthesis functionalization provides greater control over surface coverage and layer thickness, improving selectivity, it is challenging and complicated, making it less appropriate for scalable or high-throughput applications. Despite these challenges, MOFs' capabilities are enhanced by their incorporation into monolithic structures, which provide better performance, efficiency, and selectivity. These hybrid materials have a lot of potential for use in pharmaceutical development, environmental monitoring, and biomolecule enrichment. However, concerns like material heterogeneity, reproducibility, and scalability limit their practical application in bioanalysis.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3503-3511"},"PeriodicalIF":3.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aptamer-based microfluidics for the detection of cancer biomarkers. 基于适配体的微流体检测癌症生物标志物。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-07-01 Epub Date: 2025-04-14 DOI: 10.1007/s00216-025-05863-7

Yi-Xin-Yue Gong, Xiao-Feng Huang, Jian Liang, Jing Xie, Lin-Sen Qing

引用次数: 0

A novel method for the determination of synthetic cathinones and related substances in postmortem blood samples using cork-based dispersive solid-phase microextraction prior to LC-MS/MS analysis. 建立了一种在LC-MS/MS分析前采用软木基分散固相微萃取法测定死后血液样品中合成卡西酮及其相关物质的新方法。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-07-01 Epub Date: 2025-05-14 DOI: 10.1007/s00216-025-05907-y

Letícia Birk, Bruno Pereira Dos Santos, Sarah Eller, Tiago Franco de Oliveira

{"title":"A novel method for the determination of synthetic cathinones and related substances in postmortem blood samples using cork-based dispersive solid-phase microextraction prior to LC-MS/MS analysis.","authors":"Letícia Birk, Bruno Pereira Dos Santos, Sarah Eller, Tiago Franco de Oliveira","doi":"10.1007/s00216-025-05907-y","DOIUrl":"10.1007/s00216-025-05907-y","url":null,"abstract":"The detection of new psychoactive substances in postmortem blood is of primary importance for the investigation of related deaths. A method using cork as a biosorbent in a solid-phase microextraction with LC-MS/MS analysis was developed and validated for the determination of synthetic cathinones and related substances in blood. The sample preparation method employed 200 µL of sample and 30 mg of cork powder, and the complete procedure was optimized using multivariate strategies. LC-MS/MS analysis was performed in gradient mode, with a total run time of 7.5 min for the determination of cathinone, ethylone, fentanyl, MDA, MDMA, mephedrone, N-ethylpentylone, and pentylone. Comprehensive validation was performed through the ASB/ANSI Standard 036. Lower limit of quantification (LLOQ) values ranged between 0.5 and 1 ng/mL, with linear ranges between LLOQ and 85 ng/mL. Accuracy and precision results were satisfactory, and significant ion suppression was found (2.9-63.4%). Applicability of the method was evaluated by the analysis of three postmortem blood samples from forensic casework, suspected to have the presence of synthetic cathinones. All samples were positive for MDMA and MDA, with concentrations between 79.2-198.1 ng/mL and 23.1-26.6 ng/mL, respectively. The greenness of the method was also evaluated by AGREEprep, with an overall score of 0.53. This study is the first to apply a cork-based extraction to blood analysis. The method has proven to be reliable and a valuable greener alternative for the determination of synthetic cathinones and related substances in postmortem blood.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3845-3855"},"PeriodicalIF":3.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SERS and colorimetric dual-mode detection of cholesterol during drug treatment of atherosclerosis. 动脉粥样硬化药物治疗中胆固醇的SERS和比色双模检测。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-07-01 Epub Date: 2025-05-22 DOI: 10.1007/s00216-025-05910-3

Yujuan Ji, Yi Ji, Yupeng Wu, Jiaqi Wang, Yu Xue, Haiqin Huang, Yanyan Yu, Dan Sun

{"title":"SERS and colorimetric dual-mode detection of cholesterol during drug treatment of atherosclerosis.","authors":"Yujuan Ji, Yi Ji, Yupeng Wu, Jiaqi Wang, Yu Xue, Haiqin Huang, Yanyan Yu, Dan Sun","doi":"10.1007/s00216-025-05910-3","DOIUrl":"10.1007/s00216-025-05910-3","url":null,"abstract":"Foam cell formation caused by cholesterol accumulation in macrophages is an early warning of atherosclerosis. Therefore, in situ and accurate determination of intracellular and extracellular cholesterol in macrophages is of great significance for the early diagnosis of atherosclerosis and the evaluation of its therapeutic effect. Herein, a surface-enhanced Raman spectroscopy (SERS) and colorimetric dual-response plasmonic nano-sensing system was established based on a double-enzyme cascade catalytic reaction to accurately monitor the changes in cholesterol levels during the occurrence and development of atherosclerosis. Cholesterol oxidase modified on gold nanoparticles can catalyze the oxidation of cholesterol to hydrogen peroxide, which oxidizes 3,3,5,5-tetramethylbenzidine (TMB) to oxidation state TMB (oxTMB) under the catalysis of horseradish peroxidase (HRP). The oxTMB showed a very strong SERS signal under the effect of plasmonic enhancement of gold nanoparticles, and the solution of oxTMB is bright blue compared to colorless TMB. Therefore, the dual-response sensing of cholesterol was realized by monitoring SERS and UV absorption signals of the final product oxTMB. Our results suggest that the free cholesterol in macrophages increases after the treatment of avasimibe, which ultimately leads to apoptosis of macrophages. Another drug, high-density lipoprotein, promoted the efflux of intracellular cholesterol and inhibited the foaming of macrophages, thus delaying the development of atherosclerosis. The colorimetric and SERS double-response plasmonic nanosensor realized highly sensitive, specific, and accurate monitoring of intracellular and extracellular cholesterol levels during drug treatment of atherosclerosis. It is expected to provide an effective tool and methodological reference for the prevention and diagnosis of atherosclerosis and a series of cardiovascular diseases related to cholesterol.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3869-3878"},"PeriodicalIF":3.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating metabolite-based molecular networking with database matching and LC-MS-guided targeted isolation for the discovery of novel chemical constituents: application to Euphorbia helioscopia L. 整合基于代谢产物的分子网络与数据库匹配和lc - ms引导的靶向分离，发现新的化学成分：在大戟上的应用。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-07-01 Epub Date: 2025-05-10 DOI: 10.1007/s00216-025-05893-1

Tianren Wu, Yaling Deng, Weijia Hu, Caihong Bai, Han Yu, Kaifeng Hu

{"title":"Integrating metabolite-based molecular networking with database matching and LC-MS-guided targeted isolation for the discovery of novel chemical constituents: application to Euphorbia helioscopia L.","authors":"Tianren Wu, Yaling Deng, Weijia Hu, Caihong Bai, Han Yu, Kaifeng Hu","doi":"10.1007/s00216-025-05893-1","DOIUrl":"10.1007/s00216-025-05893-1","url":null,"abstract":"Molecular networking (MN) analysis facilitates the targeted discovery of novel constituents and enhances the understanding of natural products. While various molecular networks could reduce the effects of redundant nodes, current research is still limited by the interference from the same and coeluted metabolites, including isotopic peaks, a variety of adduct ions, in-source fragmentations, and dehydration. This research proposes a novel strategy: stratified precursor lists (SPLs)-guided Metabolite-Based Molecular Networking (MBMN), which ensures a high-quality MS2 spectrum for each metabolite precursor due to the absence of retention time overlap with other coeluted metabolites, and each node represents a unique metabolite. By collecting over 40 MS2 databases from multiple online platforms and public databases, an integrated MS2 database (IM2DB) containing more than two million MS2 fragmentation data was constructed. In addition, a customized MS1 database (M1DB) of reported compounds was also created. Nodes representing known compounds were annotated compared to the IM2DB and M1DB. Combining with MBMN analysis significantly enhances compound identification and characterization, thereby facilitating the discerning of potential novel constituents. To demonstrate the applicability of this strategy, we selected Euphorbia helioscopia L. as an example. 135 nodes were annotated, and three reference nodes were obtained. From the selected 35 target nodes, 10 purified compounds were isolated and elucidated. Among these, three were identified as novel compounds, while the remaining nine were discovered for the first time in Euphorbia helioscopia L. By using this strategy, we can effectively minimize the interference from redundant nodes and discover potentially new compounds.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3685-3702"},"PeriodicalIF":3.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dispersive solid-phase extraction as sample pretreatment for determination of chemotherapeutic agents revumenib and venetoclax by HPLC-DAD. 分散固相萃取法测定化疗药物revumenib和venetoclax的前处理方法。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-07-01 Epub Date: 2025-05-05 DOI: 10.1007/s00216-025-05895-z

Sergio Fernández-Trujillo, Gregorio Castañeda-Peñalvo, Juana Rodríguez-Flores, Rosa Del Carmen Rodríguez Martín-Doimeadios

{"title":"Dispersive solid-phase extraction as sample pretreatment for determination of chemotherapeutic agents revumenib and venetoclax by HPLC-DAD.","authors":"Sergio Fernández-Trujillo, Gregorio Castañeda-Peñalvo, Juana Rodríguez-Flores, Rosa Del Carmen Rodríguez Martín-Doimeadios","doi":"10.1007/s00216-025-05895-z","DOIUrl":"10.1007/s00216-025-05895-z","url":null,"abstract":"The first nanotechnology-based innovative analytical strategy by means of dispersive solid-phase extraction (DSPE) as a necessary sample treatment approach for the simultaneous determination of revumenib and venetoclax in complex biological matrices such as human blood serum prior to high-performance liquid chromatography hyphenated to diode-array detector (HPLC-DAD) is reported. Several carbon nanotubes were rigorously evaluated as DSPE sorbents to obtain an adequate extraction and preconcentration of these chemotherapeutic agents. The best findings were 8 mg of pristine multi-walled carbon nanotubes (MWCNTs) in borate buffer (pH 10; 10 mM). Under optimized chromatographic conditions, the detection, identification, and determination of both anticancer agents and trazodone (internal standard) were done in less than 8 min of analysis. The HPLC-DAD separation was carried out in a C18 reversed-phase column with a mobile phase including ammonium acetate (pH 7; 10 mM) and methanol throughout a gradient elution mode with a sample flow rate of 0.9 mL min-1 and a column temperature of 26°C. The preconcentration factor achieved was 1.7 so the limits of detection and quantification were 0.8 μg L-1 and 2.6 μg L-1 for revumenib and 0.7 μg L-1 and 2.3 μg L-1 for venetoclax in serum samples. The proposed strategy stands up as an interesting approach for therapeutic drug monitoring in AML patients moving away from a \"one-size-fits-all\" approach.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3703-3714"},"PeriodicalIF":3.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating inkjet dispenser/liquid vortex capture-mass spectrometry for single-cell metabolomics in Hep G2 steatosis caused by tamoxifen. 评价喷墨点胶/液体涡流捕获-质谱法在他莫昔芬引起的hepg2脂肪变性中的单细胞代谢组学。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-07-01 Epub Date: 2025-05-05 DOI: 10.1007/s00216-025-05885-1

Stephen C Zambrzycki, Vilmos Kertesz, John F Cahill

{"title":"Evaluating inkjet dispenser/liquid vortex capture-mass spectrometry for single-cell metabolomics in Hep G2 steatosis caused by tamoxifen.","authors":"Stephen C Zambrzycki, Vilmos Kertesz, John F Cahill","doi":"10.1007/s00216-025-05885-1","DOIUrl":"10.1007/s00216-025-05885-1","url":null,"abstract":"Single-cell mass spectrometry (MS) is advancing our understanding of metabolic pathways in heterogeneous cell populations; however, many techniques are slow or require disruptive sample preparations. This study evaluated coupling a modified HP D100 single-cell inkjet dispenser with liquid vortex capture-mass spectrometry (D100/LVC-MS). The D100 is a single-cell inkjet dispenser capable of titrating solutions and isolating single cells via disposable cassettes equipped with microfluidic channels and an impedance sensor. The LVC-MS enables high-throughput capture, lysis, and ionization of analytes for mass spectrometric analysis. The D100/LVC-MS system was characterized through titration and single-cell experiments. Propranolol titration demonstrated linearity across a broad concentration range using the D100/LVC-MS system. Additionally, Hep G2 hepatocarcinoma cells and Chlamydomonas reinhardtii algae were used to showcase the D100's high-throughput or low-buffer-volume single-cell dispensing strategies. The D100/LVC-MS system's performance was validated by evaluating tamoxifen-induced steatosis in Hep G2 cells. Tamoxifen, associated with nonalcoholic fatty liver disease in breast cancer patients following long-term use, was tested in Hep G2 cells at 20 µM for 72 h against DMSO-treated controls. High-throughput analysis of 500 cells per condition, completed in 25 min per run, demonstrated the system's efficiency. The D100/LVC-MS system simultaneously quantified tamoxifen and measured triglycerides and phosphatidylcholines. Triglycerides were upregulated in the tamoxifen-treated cells and the results indicated two distinct cell populations, differing in tamoxifen and phosphatidylcholines levels, suggesting heterogeneity within the treated population. These findings highlight the D100/LVC-MS system as a cost-effective, high-throughput platform for single-cell metabolomics and lipidomics, with significant potential for evaluating metabolic alterations.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"3597-3609"},"PeriodicalIF":3.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0