Analytical and Bioanalytical Chemistry最新文献

{"title":"Analysis of multi-disulfide bridges for insulin aspart by stepwise reduction and differentiated alkylation.","authors":"Xinling Cui, Xiaoguang Meng, Zhishang Hu, Peize Wu, Huan Yao, Ming Li, Ling Lin","doi":"10.1007/s00216-024-05597-y","DOIUrl":"10.1007/s00216-024-05597-y","url":null,"abstract":"<p><p>Disulfide bridge, an important post-translation modification in protein, plays a key role in stabilizing three-dimensional structure of proteins, maintaining correct folded conformation, and thus regulating the biological activities. Disulfide bridge assignment is essential to understand the essence of life process and to develop protein pharmaceutical. In this study, a novel method termed as stepwise reduction and differentiated alkylation (SRDA) was developed analyzing disulfide connectivity for proteins. As a demonstration, three disulfide bridges in insulin aspart were successfully characterized using this SRDA method combined with LC-HRMSⁿ. Firstly, tris (2-carboxyethyl) phosphine (TCEP) was used to partially reduce disulfide bridges with N-ethylmaleimide (NEM) used to block the generated free thiol. Then, dithiothreitol (DTT) was used to reduce the rest disulfide bonds with iodoacetamide (IAM) used to block the newly generated free thiol. After that, an LC-HRMSⁿ method was established to assign disulfide connectivity for an insulin aspart study material, based on the different mass shifts arising from differentiated alkylation. Moreover, this approach allows for the quantitative analysis of various disulfide bond pairings, which can be applied to studies on the consistency and stability among different batches of samples. The results show that SRDA is a valuable tool for reliable quality control and quality assessment of disulfide-rich proteins such as insulin analogues.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6725-6733"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel bioassays based on 3D-printed device for sensing of hypoxia and p53 pathway in 3D cell models.","authors":"Maria Maddalena Calabretta, Maura Ferri, Annalisa Tassoni, Stefania Maiello, Elisa Michelini","doi":"10.1007/s00216-024-05606-0","DOIUrl":"10.1007/s00216-024-05606-0","url":null,"abstract":"<p><p>Cell-based assays are widely exploited for drug screening and biosensing, providing useful information about bioactivity of target analytes and complex biological samples. It is well recognized that 3D cell models are required to achieve highly valuable information, also from the perspective of replacing animal models. However, bioassays relying on 3D cell models are generally highly demanding in terms of facilities, equipment, and skilled personnel requirements. To reduce cost, increase sustainability, and provide a flexible 3D cell-based platform for bioassays, we here report a novel approach based on a 3D-printed microtissue device. To assess the suitability of this strategy for reporter gene technology, we selected to monitor two molecular pathways which were of interest in several applications, hypoxia signaling and the p53 pathway. The investigation of such pathways is highly relevant in fields spanning from drug screening to bioactivity monitoring for industrial by-product valorization. Microtissues of human hepatocarcinoma (HepG2) and human embryonic kidney (Hek293T) cell lines were obtained with a low-cost and sustainable chip platform and bioassays were developed to monitor the hypoxia-inducible factors (HIFs) and the p53 tumor suppressor pathway. HepG2 and Hek293T 3D cell models were genetically engineered to express the Luc2P from Photinus pyralis firefly either under the regulation of p53 or HIF response elements. The bioassays allowed quantitative assessment of hypoxia and tumoral activity with 1,10-phenanthroline for HIF and with doxorubicin for p53 pathway activation, respectively, showing good potential for applications of this sustainable and low-cost 3D-printed microfluidic platform for bioactivity analyses, drug screening, and precision medicine.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6819-6826"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of a smartphone-compatible MIP-based sensor for bisphenol A determination in wastewater samples.","authors":"Daria Yarynka, Anton Honcharenko, Larysa Gorbach, Elena Piletska, Sergey Piletsky, Oleksandr Brovko, Tetyana Sergeyeva","doi":"10.1007/s00216-024-05616-y","DOIUrl":"10.1007/s00216-024-05616-y","url":null,"abstract":"<p><p>A handheld smartphone-compatible molecularly imprinted polymer (MIP)-based sensor was developed for the analysis of bisphenol A (BPA) in wastewater samples. Sensing elements based on ethylene glycol methacrylate phosphate (EGMP)-containing MIP films were designed and optimized using molecular dynamics simulations. The highly porous MIP films were synthesized via in situ polymerization, employing a fragment-based approach. The colorimetric response was based on the 4-aminoantipyrine method, while the MIP films were further utilized to detect BPA with a smartphone. The proposed sensor exhibited a wide linear range from 5 to 250 μM, with a limit of detection (LOD) of 5 μM (S/N = 3). Furthermore, the designed analytical system demonstrated excellent analytical performance in terms of selectivity, stability, and reproducibility. During sensor validation, real wastewater samples were successfully tested for BPA, showcasing the feasibility of the smartphone-compatible MIP-based sensor. Recovery values of 87.1-114.6% underscored the efficacy and reliability of the developed sensor system.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7121-7129"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel biomimetic nanoplasmonic sensor for rapid and accurate evaluation of checkpoint inhibitor immunotherapy.","authors":"Razia Batool, Maria Soler, Rukmani Singh, Laura M Lechuga","doi":"10.1007/s00216-024-05398-3","DOIUrl":"10.1007/s00216-024-05398-3","url":null,"abstract":"<p><p>Immune checkpoint inhibitors (ICIs) emerged as promising immunotherapies for cancer treatment, harnessing the patient's immune system to fight and eliminate tumor cells. However, despite their potential and proven efficacies, checkpoint inhibitors still face important challenges such as the tumor heterogeneity and resistance mechanisms, and the complex in vitro testing, which limits their widespread applicability and implementation to treat cancer. To address these challenges, we propose a novel analytical technique utilizing biomimetic label-free nanoplasmonic biosensors for rapid and reliable screening and evaluation of checkpoint inhibitors. We have designed and fabricated a low-density nanostructured plasmonic sensor based on gold nanodisks that enables the direct formation of a functional supported lipid bilayer, which acts as an artificial cell membrane for tumor ligand immobilization. With this biomimetic scaffold, our biosensing approach provides real-time, highly sensitive analysis of immune checkpoint pathways and direct assessment of the blocking effects of monoclonal antibodies in less than 20 min/test. We demonstrate the accuracy of our biomimetic sensor for the study of the programmed cell death protein 1 (PD1) checkpoint pathway, achieving a limit of detection of 6.7 ng/mL for direct PD1/PD-L1 interaction monitoring. Besides, we have performed dose-response inhibition curves for an anti-PD1 monoclonal antibody, obtaining a half maximal inhibitory concentration (IC₅₀) of 0.43 nM, within the same range than those obtained with conventional techniques. Our biomimetic sensor platform combines the potential of plasmonic technologies for rapid label-free analysis with the reliability of cell-based assay in terms of ligand mobility. The biosensor is integrated in a compact user-friendly device for the straightforward implementation in biomedical and pharmaceutical laboratories.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7295-7304"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141430997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-supported electrochemical sensor based on uniform palladium nanoparticles functionalized porous graphene film for monitoring H₂O₂ released from living cells.","authors":"Jiangbo Xi, Yue Zhang, Ting Ye, Jian Xiao, Jun Fang, Minghui Han, Anshun Zhao, Yan Zhang","doi":"10.1007/s00216-024-05600-6","DOIUrl":"10.1007/s00216-024-05600-6","url":null,"abstract":"<p><p>Graphene film has been considered a promising material for the construction of self-supported electrodes due to its favorable flexibility and high conductivity. However, the film fabricated from pristine graphene or conventional graphene sheet reduced graphene oxide processes limited electrocatalytic performance. Decorating active metal species or incorporating heteroatoms into the graphene framework have been proved to be effective methods to enhance the electrocatalytic efficiency of graphene film-based self-supported electrodes. Herein, we present a freestanding electrode composed of uniform Pd nanoparticles decorating N,S co-doped porous graphene film (Pd/NSPGF) and explore its practical application in differentiating various human colon cell types by in situ tracking the amount of H₂O₂ secreted from live cells. Our findings reveal that, on the one hand, the NSPGF has abundant surface and inner pores, which promote active site exposure, and mass diffusion during electrochemical reactions; on the other hand, the substitutional doping of the graphene framework with heteroatoms (e.g., N or S) can tailor its electronic and chemical properties, and facilitate the uniform loading of high-density Pd nanoparticles. Moreover, the intrinsic activity of Pd/NSPGF is regulated by the interaction of Pd nanoparticles with the NSPGF support. Taking the advantages of morphology and composition, the self-supported Pd/NSPGF electrode displays remarkable electrochemical performance with a wide linear range up to 2.0 mM, low detection limit of 0.1 μM (S/N = 3), high sensitivity of 665 µA cm^-2 mM^-1, and good selectivity. When applied in real-time tracking of the H₂O₂ released from normal human colon epithelial cells and human colorectal cancer cells, the Pd/NSPGF-based electrochemical sensing system can distinguish the cell types by testing the number of extracellular H₂O₂ molecules released per cell, which holds considerable potential for early detection and monitoring of disease-related clinical specimens.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6995-7006"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semi-supervised comparative learning compensation method for chemical gas sensor drift.","authors":"Lijian Xiong, Meng Wang, Zhaoshuai Zhu, Meng He, Yuxin Hou, Xiuying Tang","doi":"10.1007/s00216-024-05577-2","DOIUrl":"10.1007/s00216-024-05577-2","url":null,"abstract":"<p><p>The gradual and unpredictable variation in chemo-sensory signal responses when exposed to the same analyte under identical conditions, commonly referred to as sensor drift, has long been recognized as one of the most serious challenges faced by chemical sensors. The traditional drift compensation method is both labor-intensive and expensive, as it requires frequent collection and labeling of gas samples for recalibration. Introducing a small number of meaningful drift calibration samples can be an attractive strategy to reduce the computational load and improve the performance of the updated classifier. However, under the influence of drift, new challenges arise due to the difference in the distribution of source and target domain data. This paper proposes a novel algorithm framework called semi-supervised contrastive learning drift compensation (SSCLDC). The framework automatically extracts high-level abstract features based on a multilayer perceptron to better represent the structure of the source data. In addition, to address the issue of data distribution differences caused by drift between the source and target domains. We add a small number of reference sample pairs into the training for semi-supervised learning. Combining a contrastive loss function that can represent the matching degree of paired samples effectively overcomes the problem of sensor drift. The Kennard-Stone sequential algorithm is used to select the representative reference sample from the set of candidate reference samples. Experiments conducted on a widely used long-term chemical gas sensor drift dataset demonstrate that the proposed method outperforms several classic drift compensation techniques, highlighting its effectiveness and practical applicability.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6827-6838"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple solution and paper-based fluorescent aptasensors for toxic metal ions, thallium(l) and lead(ll).","authors":"Sathya Srinivasan, Velu Ranganathan, Erin M McConnell, Maria C DeRosa","doi":"10.1007/s00216-024-05614-0","DOIUrl":"10.1007/s00216-024-05614-0","url":null,"abstract":"<p><p>Heavy metal ions, such as thallium(I) and lead(II) are environmental toxicants known to cause a severe threat to human and ecosystem health. This work investigates aptamers and intercalating chromophore-based complexes for the detection of these toxic species. In one method, a selective label-free \"turn-on\" biosensor was developed using a G-quadruplex-intercalating agent, berberine. Fluorescence, melting temperature (T_m), and circular dichroism analysis confirmed the affinity and selectivity results, illustrating the potential of these aptasensor methods for improving detection limits. These fluorescence assays were found to perform with a detection limit of 3.4 μM for Tl(I) and 0.84 nM for Pb(II). Furthermore, the assays were challenged successfully with Tl(I) and Pb(II) spiked into river water samples. We next developed paper-based fluorescent assays for Tl(I) and Pb(II), where the aptamer/berberine complex was spotted onto the paper test zone. When Tl(I) or Pb(II) ions solutions were spotted onto the top of the test zone and the spot was illuminated with a portable UV light (365 nm), a strong green fluorescence could be easily visualized with the naked eye. The lowest detection limits achieved with these fluorescent paper-based assays for Tl(I) and Pb(II) were 1.1 nM and 1.6 nM, respectively. The two fluorescent approaches presented here have the potential to be the basis of rapid, fast, and cost-efficient screening assays for these toxic species.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7099-7108"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative assessment of poly- and perfluoroalkyl substances (PFASs) in aqueous film forming foam (AFFF)-impacted soils: a comparison of analytical protocols.","authors":"Nicholas Gonda, Chuhui Zhang, Dylan Tepedelen, Adam Smith, Charles Schaefer, Christopher P Higgins","doi":"10.1007/s00216-024-05585-2","DOIUrl":"10.1007/s00216-024-05585-2","url":null,"abstract":"<p><p>Quantitatively assessing all per- and poly fluoroalkyl substances (PFASs) in an environmental sample, particularly soils impacted by aqueous film forming foams (AFFFs), has proven to be a challenge. To make such an assessment, a comprehensive sample processing procedure and analytical tool must be used. However, doubts remain whether current analytical tools such as high-resolution mass spectrometry (HRMS) with targeted quantitation and semi-quantitative analysis of suspects (Semi-Q HRMS) or total organic fluorine (TOF) are capable of accurately quantifying all non-polymeric PFASs in a sample. Further, current comprehensive soil PFAS HRMS methods are incompatible with TOF, preventing direct comparisons of the approaches. To enable direct comparisons, a soil sample processing procedure that is comprehensive as well as compatible with multiple analytical tools is needed. In this study, we assessed the performance of a previously developed soil PFAS method, EPA Method 1633, and a hybrid solid phase extraction (SPE)-based method for characterizing AFFF-impacted soil composites while maintaining compatibility with multiple analytical tools (i.e., Semi-Q HRMS and TOF). Comparative results for AFFF-impacted soil composites indicate analysis via EPA Method 1633 (as compared to the novel hybrid method) results in maybe up to 75% of the PFAS mass being missed by only analyzing for compounds listed in EPA Method 1633. Simply expanding the EPA Method 1633 analyte list was insufficient to account for the missing mass: up to 69% of the PFAS mass was still missed because of EPA Method 1633's extraction and cleanup bias. Additionally, the novel method developed offers a more comprehensive analysis with minimal reductions to sensitivity when compared to those reported in EPA Method 1633, with limits of quantification ranging from 0.12 to 2.4 ng/g as compared to 0.16-4.0 ng/g, respectively. For these reasons, an alternative hybrid SPE-based method is proposed for comprehensive evaluation of PFASs in AFFF-impacted soils.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6879-6892"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemiluminescent imaging of a NADH-based enzymatic reaction confined within giant liposomes.","authors":"Fatma Ben Trad, Bixente Carré, Jérôme Delacotte, Frédéric Lemaître, Manon Guille-Collignon, Stéphane Arbault, Neso Sojic, Eric Labbé, Olivier Buriez","doi":"10.1007/s00216-024-05133-y","DOIUrl":"10.1007/s00216-024-05133-y","url":null,"abstract":"<p><p>Herein, transient releases either from NADH-loaded liposomes or enzymatic reactions confined in giant liposomes were imaged by electrochemiluminescence (ECL). NADH was first encapsulated with the [Ru(bpy)₃]²⁺ luminophore inside giant liposomes (around 100 µm in diameter) made of DOPC/DOPG phospholipids (i.e., 1,2-dioleolyl-sn-glycero-3-phosphocholine/1,2-dioleoyl-sn-glycerol-3-phospho-(1'-rac-glycerol) sodium salt) on their inner- and outer-leaflet, respectively. Then, membrane permeabilization triggered upon contact between the liposome and a polarized ITO electrode surface and ECL was locally generated. Combination of amperometry, photoluminescence, and ECL provided a comprehensive monitoring of a single liposome opening and content release. In a second part, the work is focused on the ECL characterization of NADH produced by glucose dehydrogenase (GDH)-catalyzed oxidation of glucose in the confined environment delimited by the liposome membrane. This was achieved by encapsulating both the ECL and catalytic reagents (i.e., the GDH, glucose, NAD⁺, and [Ru(bpy)₃]²⁺) in the liposome. In accordance with the results obtained, NADH can be used as a biologically compatible ECL co-reactant to image membrane permeabilization events of giant liposomes. Under these conditions, the ECL signal duration was rather long (around 10 s). Since many enzymatic reactions involve the NADH/NAD⁺ redox couple, this work opens up interesting prospects for the characterization of enzymatic reactions taking place notably in artificial cells and in confined environments.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7385-7394"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139471596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An easy and sensitive assay for acetohydroxyacid synthases based on the simultaneous detection of substrates and products in a single step.","authors":"Annika Engelhardt, Marco Ebeling, Elisabeth Kaltenegger, Dorothee Langel, Dietrich Ober","doi":"10.1007/s00216-024-05613-1","DOIUrl":"10.1007/s00216-024-05613-1","url":null,"abstract":"<p><p>Acetohydroxyacid synthase (AHAS, EC 2.2.1.6) catalyzes the first step in the synthesis of the branched-chain amino acids valine, leucine, and isoleucine, pathways being present in plants and microorganisms, but not in animals. Thus, AHAS is an important target for numerous herbicides and, more recently, for the development of antimicrobial agents. The need to develop new and optimized herbicides and pharmaceuticals requires a detailed understanding of the biochemistry of AHAS. AHAS transfers an activated two-carbon moiety derived from pyruvate to either pyruvate or 2-oxobutyrate as acceptor substrates, forming 2-acetolactate or 2-acetohydroxy-2-butyrate, respectively. Various methods have been described in the literature to biochemically characterize AHAS with respect to substrate preferences, substrate specificity, or kinetic parameters. However, the simultaneous detection and quantification of substrates and unstable products of the AHAS-catalyzed reaction have always been a challenge. Using AHAS isoform II from Escherichia coli, we have developed a sensitive assay for AHAS-catalyzed reactions that uses derivatization with ethyl chloroformate to stabilize and volatilize all reactants in the aqueous solution and detect them by gas chromatography coupled to flame ionization detection or mass spectrometry. This assay allows us to characterize the product formation in reactions in single and dual substrate reactions and the substrate specificity of AHAS, and to reinterpret previous biochemical observations. This assay is not limited to the AHAS-catalyzed reactions, but should be applicable to studies of many metabolic pathways.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7085-7098"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}