Analytical and Bioanalytical Chemistry最新文献

{"title":"Aerobic oxidation catalyst vanadyl acetylacetonate boosts luminol chemiluminescence for sensitive detection of alkaline phosphatase and ascorbic acid.","authors":"Wei Zhang, Yequan Chen, Kaixiang Ji, Zhiyong Dong, Abubakar Abdussalam, Yuriy T Zholudov, Wenyue Gao, Yu Tian, Guobao Xu","doi":"10.1007/s00216-025-05827-x","DOIUrl":"https://doi.org/10.1007/s00216-025-05827-x","url":null,"abstract":"<p><p>Vanadyl acetylacetonate, VO(acac)₂, is a well-known environmentally friendly catalyst for aerobic oxidations. It has been exploited as an efficient co-reactant of luminol chemiluminescence (CL) in aqueous media for the first time. The luminol-VO(acac)₂ system generates an intense CL and ascorbic acid can quench its CL remarkably. Based on the strong quenching effect of ascorbic acid, the luminol-VO(acac)₂ CL was used in the quantification of clinically important ascorbic acid and alkaline phosphatase (ALP) by enzymatic conversion of L-ascorbic acid 2-phosphate into ascorbic acid. The linear ranges for detecting ascorbic acid and alkaline phosphatase are 1 to 100 μM and 0.005 to 200 U/L, respectively. The limits of detection of ascorbic acid and alkaline phosphatase are 0.5 μM and 0.005 U/L, respectively. The proposed CL method has shown high selectivity for the ALP among the tested biomolecules and metal ions. It also shows good recoveries in human serum samples.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite biomarkers of screening neonatal congenital hypothyroidism based on dried blood spot metabolomics.","authors":"Xingyu Guo, Feng Suo, Yuting Wang, Di Yu, Yi Wang, Bulian Dong, Lingshan Gou, Xinhui Gan, Benjing Wang, Chaowen Yu, Xiaoxiang Xie, Dandan Linghu, Xinyu Liu, Maosheng Gu, Guowang Xu","doi":"10.1007/s00216-025-05828-w","DOIUrl":"https://doi.org/10.1007/s00216-025-05828-w","url":null,"abstract":"<p><p>Congenital hypothyroidism (CH) can be detected early during thyroid-stimulating hormone (TSH) screening based on dried blood spot (DBS) samples. However, it falls short in differentiating between neonates with elevated TSH levels and those with CH, which leads to many neonates undergoing secondary diagnosis through venous blood sampling. Metabolomics was used to analyze metabolic alterations in neonates with CH, and identify DBS-based metabolite biomarkers for CH screening to reduce secondary diagnosis. Based on non-targeted metabolomics, the metabolic alterations in neonates with CH were analyzed in a discovery set and novel biomarkers were identified. The results of the discovery set revealed that the metabolic alterations in neonates with CH were primarily in amino acid and lipid metabolism, and identified novel metabolite biomarkers were thyroxine, 2-piperidinone, and PC (14:0/20:4). Then, these biomarkers were validated in a validation set, and the screening performance was still satisfactory. A rapid 3.5-min targeted method for three potential biomarkers was further developed and used to analyze a confirmation set. Analysis of the confirmation set re-validated the reliability of the biomarkers, and a biomarker combinational model equation and an appropriate cutoff value were defined. Each set of DBS samples included neonates with health, hyperthyrotropinemia, and CH. The novel metabolite biomarkers from DBS samples demonstrate significant potential for CH screening in neonates, effectively reducing the requirement associated with secondary diagnosis of mis-screened neonates.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of reference materials for dietary supplements-analytical challenges, use, limitations, and future needs.","authors":"Stephen A Wise, Sanem Hosbas Coskun, Hugh V Hayes, Walter B Wilson, Jacolin A Murray, J Andreas Lippert, Carolyn Q Burdette, Michele M Schantz, Karen E Murphy, Steven J Christopher, Lee L Yu, Catherine A Rimmer, Stefan M Pasiakos, Adam J Kuszak","doi":"10.1007/s00216-025-05787-2","DOIUrl":"https://doi.org/10.1007/s00216-025-05787-2","url":null,"abstract":"<p><p>For two decades, the National Institute of Standards and Technology (NIST) and the National Institutes of Health Office of Dietary Supplements have collaborated to develop dietary supplement-matrix reference materials. During the first decade, NIST developed over 20 botanical and non-botanical dietary supplement Standard Reference Materials (SRMs^®) using multiple analytical techniques to assign values for selected marker compounds and toxic elements. In the past decade, NIST has expanded the scope of materials available, and other producers of certified reference materials (CRMs) have joined to provide a limited number of additional materials. This review describes briefly the first decade in the development of CRMs for dietary supplements, primarily botanical dietary supplement ingredients (e.g., ginkgo, green tea, saw palmetto, St. Johns' wort, botanical oils, berries, and soy) and a popular multivitamin/multimineral (MVM) SRM. We discuss the analytical challenges in producing these materials and how these materials established a model for the next generation of CRMs. The second generation of dietary supplement CRMs/RMs, consisting primarily of botanical matrices, calibration solutions, and new and replacement MVM CRMs, is discussed in greater detail including improvements based on experiences from the first decade and potential future needs and developments in this emerging reference material research sector.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topographic imaging with automatic z-axis correction of Brassica oleracea var. viridis leaves by IR-MALDESI mass spectrometry imaging.","authors":"Quinn Mills, Sarah M Ashbacher, Alexandria L Sohn, David C Muddiman","doi":"10.1007/s00216-025-05820-4","DOIUrl":"https://doi.org/10.1007/s00216-025-05820-4","url":null,"abstract":"<p><p>Mass spectrometry (MS) is a versatile technique for elucidating the chemical composition of biological samples. Beyond analysis of crude extracts, MS can be further applied to spatially resolve compounds across the area of a sample with a technique called mass spectrometry imaging (MSI). The infrared matrix-assisted laser desorption ionization (IR-MALDESI) platform combines elements of matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) to enable MSI of mammalian tissue using endogenous water in the sample as a matrix. For laser-based techniques such as IR-MALDESI, changes in topography across the sample surface cause inconsistent ablation as the sample surface moves above and below the focal plane of the laser. The localization of chemical species in plants reveals crucial information about metabolic processes as reported by Nemes and Vertes (Anal. Chem. 79 (21), 8098-8106, 2007) and biosynthetic pathways by Zou et al. (Trends in Plant Science, 2024) and can even inform selective breeding of crops as discussed by Sakurai (Breed Sci 72 (1), 56-65, 2022); however, leaf topography raises a unique challenge. Features such as veins and trichomes exhibit unique topography, but flattening risks delocalization of analytes and activation of unwanted signaling pathways, and transferring metabolites to a membrane for indirect analysis may incur delocalization and limit metabolomic coverage. To overcome these challenges, a chromatic confocal sensor probe (CA probe) was incorporated for IR-MALDESI-MSI of sections of a collard (Brassica oleracea var. viridis) leaf. The CA probe measures the height at all points of the sample, and automatic z-axis corrections (AzC) are generated from height differences to continuously raise and lower the stage. These stage height corrections keep the sample surface in focus of the laser for the duration of analysis. This method has been applied to relatively homogenous samples, but has not yet been characterized on heterogeneous leaf tissue with considerable topography. Herein, data quality is compared between MSI analyses with and without AzC applied, focusing on the localization of analytes known to be concentrated in different layers of collard leaves.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UPLC-Q-TOF-MS/MS and NMR studies for the structural characterization of degradation impurities of rimegepant.","authors":"Bhuvaneshwari Vuyyala, Tarzan Mohanta, Sai Ram Prasad Kollu, Jithender G Reddy, Debasish Swain","doi":"10.1007/s00216-025-05811-5","DOIUrl":"https://doi.org/10.1007/s00216-025-05811-5","url":null,"abstract":"<p><p>Rimegepant is a type of medication classified as a calcitonin gene-related peptide (CGRP) receptor antagonist, used to treat migraines. The present research focused to identify and characterize its degradation products using the liquid chromatography-high-resolution mass spectrometry (Q-TOF-HRMS) and nuclear magnetic resonance spectroscopy (NMR). The degradation profile of rimegepant was evaluated using forced degradation studies, which included exposure to hydrolysis, oxidation, photolysis, and thermal conditions. The drug degraded under hydrolytic and oxidative conditions whereas it was found to be stable under photolytic and thermal conditions. An Acquity CSH C18 column (2.1 × 100 mm; 1.7 µm) was used to separate the analytes using a mobile phase of 0.1% formic acid in water and 0.1% formic acid in acetonitrile eluting in gradient mode at a flow rate of 0.3 mL/min. The degradation products were separated and identified using the mass fragmentation and accurate mass information obtained from LC-MS. A preparative HPLC was used to isolate the major degradation products for structural identification through 1D and 2D NMR studies. According to ICH Q2 (R1) guidelines, the developed UHPLC-PDA method was validated for its accuracy, precision, linearity, and specificity.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-gradient microstructured hybrid microfluidic chip for rare tumor cell capture.","authors":"Wen Ding, Wu Ye, Huayan Liu, Jianbo Yang, Chengxing Chu, Huancheng Zhu, Jiakang Wang, Luping Zhou, Ming Zhao, Ming Liu","doi":"10.1007/s00216-025-05825-z","DOIUrl":"https://doi.org/10.1007/s00216-025-05825-z","url":null,"abstract":"<p><p>Cancer remains the leading cause of death worldwide, and early detection can significantly reduce patient mortality. Circulating tumor cells (CTCs), which are tumor cells shed from the primary tumor and transported to distant sites through the bloodstream, are key biomarkers for cancer diagnosis and contain critical information reflecting the primary tumor, making them important for monitoring cancer progression. Microfluidic chips utilizing a purely physical capture technique based on the size and deformability differences between CTCs and other blood cells have proven to be effective in capturing CTCs. This study investigates three high-gradient microstructured hybrid microfluidic chips (HGMH-Chips), each incorporating a microarray structure and a distinct geometric gradient design: linear, sawtooth, and waveform. Multiphysics simulations revealed significant differences in pressure distribution among the chip configurations. Notably, the sawtooth design exhibited a more uniform pressure drop, with only 25% of the particles in the high-pressure region. We employed two cancer cell lines (MDA-MB-231 and A549) to evaluate the chip's capture capability. Additionally, we compared the capture efficiency and cell viability across the three designs in a single cancer cell system. Experimental results demonstrated that the sawtooth chip achieved a capture efficiency of up to 70%. When applied to mixed samples containing leukocytes, the high-gradient design exhibited a capture purity of up to 98%, effectively isolating a small number of cancer cells from complex samples. This model holds promise for the capture of CTCs in complex systems. Furthermore, the microarray structure aids in stabilizing the captured cancer cells, enhancing separation efficiency. This study presents a novel chip structure design for tumor cell capture, which holds promise for improving the capture of tumor cells in complex biological samples.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of bovine milk-derived extracellular vesicles via a capillary-channeled polymer (C-CP) fiber stationary phase.","authors":"Carolina Mata, Jerisa M Pimentel, Kun Huang, Alexis Stamatikos, R Kenneth Marcus","doi":"10.1007/s00216-025-05824-0","DOIUrl":"https://doi.org/10.1007/s00216-025-05824-0","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are released by all cell types into the extracellular environment. A subset of EVs, known as exosomes, range in size from 30 to 200 nm and are of biochemical interest due to their function as vehicles of intercellular communication. Their ability to transport proteinaceous species and genetic material at the cellular level makes them prime candidates as vectors in gene therapies. Focusing on biotherapeutics, bovine milk-derived extracellular vesicles (MDEVs) hold particular promise as an alternative to other exosome sources for therapeutics delivery. Bovine milk poses unique challenges due to the complex colloidal matrix, composed predominantly of fats and proteins like casein, which form micelles that exhibit exosome-like characteristics, specifically size and density. When faced with complex matrices like milk, conventional size/density-based isolation methods including ultracentrifugation and size exclusion chromatography struggle to provide high purity yields on practical time and cost scales. When paired with a stepwise hydrophobic interaction chromatography (HIC) gradient, polyester (PET) capillary-channeled polymer (C-CP) fibers in column and spin-down tips formats have been used effectively to isolate exosomes from highly diverse sources. Here, PET C-CP fiber columns are demonstrated to isolate MDEVs from pre-treated raw milk, yielding concentrations of 1.5 × 10¹⁰ particles mL⁻¹ with purities of ~2 × 10¹⁰ EVs µg^-1 protein in less than 20 min. The efficacy of the isolation process is verified by a suite of characterization methods. Implementing PET C-CP fiber columns for MDEV isolation addresses the challenges associated with conventional isolation methods, holding promise for scale-up towards therapeutic applications.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of a certified reference material of toxic elements in cannabis leaves.","authors":"Adriana Rodriguez, Cristhian Paredes, Elianna Castillo","doi":"10.1007/s00216-025-05809-z","DOIUrl":"https://doi.org/10.1007/s00216-025-05809-z","url":null,"abstract":"<p><p>The quantification of harmful elements in cannabis is a relevant analytical task that requires metrological tools to ensure the reliability of the measurement results. This work reports the preparation and characterization of a certified reference material (CRM) for toxic elements-arsenic (As), cadmium (Cd), and lead (Pb)-in pulverized, lyophilized cannabis leaf tissue. To prepare the CRM INM-040-1, a portion of dried ground cannabis vegetal material was spiked with the elements at mass fraction values close to the levels of interest in relevant regulations for this kind of matrix: 0.34 mg/kg for arsenic, 0.34 mg/kg for cadmium, and 0.66 mg/kg for lead. The elements were quantified by inductively coupled plasma mass spectrometry (ICP-MS) in combination with graphite furnace atomic absorption spectroscopy (GF-AAS, for cadmium and lead) or hydride generation atomic absorption spectroscopy (HG-AAS, for arsenic). The analytical calibration was done by gravimetric standard addition for ICP-MS and GF-AAS, while bracketing calibration was used for HG-AAS. Furthermore, internal standard correction was used during ICP-MS measurements. The analytical methods were validated to demonstrate their fitness for purpose. The preparation variables of the CRM (particle size, drying treatment, and spiking conditions) were studied to improve the homogeneity of the CRM. The mass fraction of the toxic elements was certified with relative standard uncertainties ranging from 4.2 to 6.9%. The uncertainty contributions considered were the elements' mass fraction measurements, the between-methods bias, the (in)homogeneity of the production batch, and the (in)stability under transport and storage conditions. This new CRM constitutes a useful tool for the laboratories assessing the harmlessness of cannabis materials, promoting humans' safety and regulatory compliance within the medicinal cannabis industry.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing solvent extraction methods for activated carbon-based passive samplers in atmospheric volatile organic compound analysis: minimizing analytical interferences from pretreatment solvents and ensuring quantitative reliability.","authors":"Se-Eun Jang, Yong-Hyun Kim","doi":"10.1007/s00216-025-05814-2","DOIUrl":"https://doi.org/10.1007/s00216-025-05814-2","url":null,"abstract":"<p><p>A passive sampler was used to effectively monitor trace volatile organic compound (VOC) concentrations in the atmosphere. VOCs are typically extracted from passive samplers using CS₂, which is a volatile and hazardous chemical that can leave residues and damage the mass spectrometry (MS) system during gas chromatography (GC)-MS. This study aims to develop and validate alternative solvent extraction methods using acetone, ethanol, n-hexane, and a solution of 99% acetone and 1% CS₂ (ATCS) for VOCs from passive samplers using a standard GC-MS system. ATCS had the highest VOC extraction efficiency with the average value of 42.4 ± 21.4%, followed by acetone at 29.9 ± 17.6%. Ethanol and n-hexane exhibited extraction efficiencies of less than 9%. Despite the ATCS extraction efficiency of less than 50%, it demonstrated excellent analytical reproducibility (relative standard deviation of 1.62 ± 0.64%) and detection limit of 20.5 ± 12.9 ppt, which was significantly lower than 1 ppb. When used to extract and analyze VOCs from ambient air samples, ATCS yielded VOC concentrations of 0.57 ± 0.33 ppb, consistent with urban air levels. The variance in the outdoor VOC concentrations was less than 0.1 ppb, confirming its high reproducibility. Thus, the ATCS solvent extraction method developed in this study enables the accurate quantification of trace VOCs below 1 ppb, reduces MS damage, and mitigates health risks to analysts using GC-MS.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of polymer monolith-MOF hybrid via surface functionalization for bioanalytical sciences.","authors":"Kinza Ali, Iqra Munawar, Sara Manan, Fatima Nawazish, Batool Fatima, Fahmida Jabeen, Adeela Saeed","doi":"10.1007/s00216-025-05822-2","DOIUrl":"https://doi.org/10.1007/s00216-025-05822-2","url":null,"abstract":"<p><p>Monoliths are versatile materials with diverse applications, and their performance can be enhanced through modifications, including the use of metal-organic frameworks (MOFs). Modified monoliths improve separation and analytical processes in various fields, with different modification methods offering distinct benefits and challenges. Directly adding MOF crystals to the polymerization mixture is straightforward and time effective, but it often results in poor dispersion and compositional heterogeneity, which compromises consistency and reproducibility, particularly in bioanalytical applications. Although layer-by-layer (LbL) development or post-synthesis functionalization provides greater control over surface coverage and layer thickness, improving selectivity, it is challenging and complicated, making it less appropriate for scalable or high-throughput applications. Despite these challenges, MOFs' capabilities are enhanced by their incorporation into monolithic structures, which provide better performance, efficiency, and selectivity. These hybrid materials have a lot of potential for use in pharmaceutical development, environmental monitoring, and biomolecule enrichment. However, concerns like material heterogeneity, reproducibility, and scalability limit their practical application in bioanalysis.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}