Analytical and Bioanalytical Chemistry最新文献_第7页

Biosensing strategies using recombinant luminescent proteins and their use for food and environmental analysis. 使用重组发光蛋白的生物传感策略及其在食品和环境分析中的应用。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-09-26 DOI: 10.1007/s00216-024-05552-x

Fernando Pradanas-González, Marta García Cortés, Bettina Glahn-Martínez, Melisa Del Barrio, Pablo Purohit, Elena Benito-Peña, Guillermo Orellana

{"title":"Biosensing strategies using recombinant luminescent proteins and their use for food and environmental analysis.","authors":"Fernando Pradanas-González, Marta García Cortés, Bettina Glahn-Martínez, Melisa Del Barrio, Pablo Purohit, Elena Benito-Peña, Guillermo Orellana","doi":"10.1007/s00216-024-05552-x","DOIUrl":"10.1007/s00216-024-05552-x","url":null,"abstract":"Progress in synthetic biology and nanotechnology plays at present a major role in the fabrication of sophisticated and miniaturized analytical devices that provide the means to tackle the need for new tools and methods for environmental and food safety. Significant research efforts have led to biosensing experiments experiencing a remarkable growth with the development and application of recombinant luminescent proteins (RLPs) being at the core of this boost. Integrating RLPs into biosensors has resulted in highly versatile detection platforms. These platforms include luminescent enzyme-linked immunosorbent assays (ELISAs), bioluminescence resonance energy transfer (BRET)-based sensors, and genetically encoded luminescent biosensors. Increased signal-to-noise ratios, rapid response times, and the ability to monitor dynamic biological processes in live cells are advantages inherent to the approaches mentioned above. Furthermore, novel fusion proteins and optimized expression systems to improve their stability, brightness, and spectral properties have enhanced the performance and pertinence of luminescent biosensors in diverse fields. This review highlights recent progress in RLP-based biosensing, showcasing their implementation for monitoring different contaminants commonly found in food and environmental samples. Future perspectives and potential challenges in these two areas of interest are also addressed, providing a comprehensive overview of the current state and a forecast of the biosensing strategies using recombinant luminescent proteins to come.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7205-7224"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The timeless value of quality peer review. 高质量同行评审的永恒价值

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-09-27 DOI: 10.1007/s00216-024-05564-7

Adam T Woolley

引用次数: 0

Simultaneous analysis of several plasticizer classes in different matrices by on-line turbulent flow chromatography-LC-MS/MS. 利用在线湍流色谱-LC-MS/MS 同时分析不同基质中的几类增塑剂。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-19 DOI: 10.1007/s00216-024-05593-2

Julio Fernández-Arribas, Sandra Callejas-Martos, Aleix Balasch, Teresa Moreno, Ethel Eljarrat

{"title":"Simultaneous analysis of several plasticizer classes in different matrices by on-line turbulent flow chromatography-LC-MS/MS.","authors":"Julio Fernández-Arribas, Sandra Callejas-Martos, Aleix Balasch, Teresa Moreno, Ethel Eljarrat","doi":"10.1007/s00216-024-05593-2","DOIUrl":"10.1007/s00216-024-05593-2","url":null,"abstract":"The development of methodologies for the determination of plasticizers is essential for assessing the environmental and human impact resulting from the use of plastics. A fast analytical method with on-line purification based on turbulent flow chromatography (TFC) coupled to tandem mass spectrometry (MS-MS) has been developed for the analysis of ten phthalates, four alternative plasticizers (including adipates and citrates), and 20 organophosphate esters (OPEs). The method has been validated for the determination of plasticizers across different matrices. Analytical parameters showed acceptable recoveries ranging between 50 and 125%, RSDs lower than 20%, and mLODs of 0.001-2.08 ng g-1 wet weight (ww), 0.002-0.30 ng g-1, and 0.001-0.93 ng m-3 for foodstuffs, face masks, and ambient air, respectively. These methodologies were applied to foodstuff samples purchased in grocery stores, reusable and self-filtering masks, and indoor air measured in different locations. Plasticizers were detected in all the analyzed samples, with values up to 22.0 μg g-1 ww, 6.78 μg g-1, and 572 ng m-3 for foodstuffs, face masks, and indoor air, respectively. The contribution of each family to the total plasticizer content varied between 1.3 and 87%, 0.5 and 98%, and 0.5 and 65% for phthalates, alternative plasticizers, and OPEs, respectively. These findings highlighted the need for analytical methodologies capable of simultaneously assessing a wide number of plasticizers with minimal extraction steps. This capability is crucial in order to obtain more conclusive insights into the impact of these pollutants on both the environment and human health, arising from different sources of exposure such as foodstuffs, plastic materials, and atmospheric air.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6957-6972"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and immobilization of novel N-glycan-binding protein for highly efficient purification and enrichment of N-glycans, N-glycopeptides, and N-glycoproteins. 新型 N-糖结合蛋白的表达和固定，用于高效纯化和富集 N-糖、N-糖肽和 N-糖蛋白。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-16 DOI: 10.1007/s00216-024-05583-4

Liang Zhang, Wenhui Wang, Yueqin Yang, Pengjie Li, Xiang Liu, Wenjie Zhu, Wei Yang, Song Wang, Yawei Lin, Xin Liu

{"title":"Expression and immobilization of novel N-glycan-binding protein for highly efficient purification and enrichment of N-glycans, N-glycopeptides, and N-glycoproteins.","authors":"Liang Zhang, Wenhui Wang, Yueqin Yang, Pengjie Li, Xiang Liu, Wenjie Zhu, Wei Yang, Song Wang, Yawei Lin, Xin Liu","doi":"10.1007/s00216-024-05583-4","DOIUrl":"10.1007/s00216-024-05583-4","url":null,"abstract":"Comprehensive and selective enrichment of N-glycans, N-glycopeptides, and N-glycoproteins prior to analysis is of great significance in N-glycomics research, reducing sample complexity, removing impurity interference, increasing sample abundance and enhancing signal intensity. However, only an Fbs1 (F-box protein that recognizes sugar chain 1) GYR variant (Fg) can enrich these N-glycomolecules solely due to its substantial binding affinity for the core pentasaccharide motif of N-glycans. Stationary phase separation is commonly used to enrich N-glycomolecules efficiently. Herein, DNA encoding the Fg was cloned into pGEX-4T-1, and the protein was expressed with a GST tag, which facilitates the convenient and efficient immobilization of recombinant GST-tagged Fg to GSH agarose resin. The yield of the GST-tagged Fg reached to 0.05 g/L after optimization of the induction condition, and the purified protein exhibited good identification ability and excellent stability for months. In particular, the immobilized GST-tagged Fg can enrich N-glycans released by PNGase F and capture derivatized N-glycans possessing an intact terminal N-acetyl glucosamine (GlcNAc). Validation of immobilized GST-tagged Fg with standard N-glycopeptides and N-glycoproteins revealed its high loading capacity, sensitivity, and selectivity. The novel immobilized GST-tagged Fg is a convenient and efficient enrichment material specific for N-glycans, N-glycopeptides, and N-glycoproteins, suggesting excellent performance and prospects for industrial application.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6859-6868"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced plasmonic scattering imaging via deep learning-based super-resolution reconstruction for exosome imaging. 通过基于深度学习的超分辨率重构增强等离子体散射成像，用于外泌体成像。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-09-24 DOI: 10.1007/s00216-024-05550-z

Zhaochen Huo, Bing Chen, Zhan Wang, Yu Li, Lei He, Boheng Hu, Haoliang Li, Pengfei Wang, Jianning Yao, Feng Xu, Ya Li, Xiaonan Yang

{"title":"Enhanced plasmonic scattering imaging via deep learning-based super-resolution reconstruction for exosome imaging.","authors":"Zhaochen Huo, Bing Chen, Zhan Wang, Yu Li, Lei He, Boheng Hu, Haoliang Li, Pengfei Wang, Jianning Yao, Feng Xu, Ya Li, Xiaonan Yang","doi":"10.1007/s00216-024-05550-z","DOIUrl":"10.1007/s00216-024-05550-z","url":null,"abstract":"Exosome analysis plays pivotal roles in various physiological and pathological processes. Plasmonic scattering microscopy (PSM) has proven to be an excellent label-free imaging platform for exosome detection. However, accurately detecting images scattered from exosomes remains a challenging task due to noise interference. Herein, we proposed an image processing strategy based on a new blind super-resolution deep learning neural network, named ESRGAN-SE, to improve the resolution of exosome PSI images. This model can obtain super-resolution reconstructed images without increasing experimental complexity. The trained model can directly generate high-resolution plasma scattering images from low-resolution images collected in experiments. The results of experiments involving the detection of light scattered by exosomes showed that the proposed super-resolution detection method has strong generalizability and robustness. Moreover, ESRGAN-SE achieved excellent results of 35.52036, 0.09081, and 8.13176 in terms of three reference-free image quality assessment metrics, respectively. These results show that the proposed network can effectively reduce image information loss, enhance mutual information between pixels, and decrease feature differentiation. And, the single-image SNR evaluation score of 3.93078 also showed that the distinction between the target and the background was significant. The suggested model lays the foundation for a potentially successful approach to imaging analysis. This approach has the potential to greatly improve the accuracy and efficiency of exosome analysis, leading to more accurate cancer diagnosis and potentially improving patient outcomes.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6773-6787"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142306872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Raman fiber-optic probe for rapid diagnosis of gastric and esophageal tumors with machine learning analysis or similarity assessments: a comparative study. 利用机器学习分析或相似性评估快速诊断胃和食管肿瘤的拉曼光纤探针：一项比较研究。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-09-25 DOI: 10.1007/s00216-024-05545-w

Shiyan Fang, Pei Xu, Siyi Wu, Zhou Chen, Junqing Yang, Haibo Xiao, Fangbao Ding, Shuchun Li, Jin Sun, Zirui He, Jian Ye, Linley Li Lin

{"title":"Raman fiber-optic probe for rapid diagnosis of gastric and esophageal tumors with machine learning analysis or similarity assessments: a comparative study.","authors":"Shiyan Fang, Pei Xu, Siyi Wu, Zhou Chen, Junqing Yang, Haibo Xiao, Fangbao Ding, Shuchun Li, Jin Sun, Zirui He, Jian Ye, Linley Li Lin","doi":"10.1007/s00216-024-05545-w","DOIUrl":"10.1007/s00216-024-05545-w","url":null,"abstract":"Gastric and esophageal cancers, the predominant forms of upper gastrointestinal malignancies, contribute significantly to global cancer mortality. Routine detection methods, including medical imaging, endoscopic examination, and pathological biopsy, often suffer from drawbacks such as low sensitivity and laborious and complex procedures. Raman spectroscopy is a non-invasive and label-free optical technique that provides highly sensitive biomolecular information to facilitate effective tumor identification. In this work, we report the use of fiber-optic Raman spectroscopy for the accurate and rapid diagnosis of gastric and esophageal cancers. Using a database of 14,000 spectra from 140 ex vivo tissue pieces of both tumor and normal tissue samples, we compare the random forest (RF) and our established Euclidean distance Raman spectroscopy (EDRS) model. The RF analysis achieves a sensitivity of 85.23% and an accuracy of 83.05% in diagnosing gastric tumors. The EDRS algorithm with improved diagnostic transparency further increases the sensitivity to 92.86% and accuracy to 89.29%. When these diagnostic protocols are extended to esophageal tumors, the RF and EDRS models achieve accuracies of 71.27% and 93.18%, respectively. Finally, we demonstrate that fewer than 20 spectra are sufficient to achieve good Raman diagnostic accuracy for both tumor tissues. This optimizes the balance between acquisition time and diagnostic performance. Our work, although conducted on ex vivo tissue models, offers valuable insights for in vivo in situ endoscopic Raman diagnosis of gastric and esophageal cancer lesions in the future. Our study provides a robust, rapid, and convenient method as a new paradigm in in vivo endoscopic medical diagnostics that integrates spectroscopic techniques and a Raman probe for detecting upper gastrointestinal malignancies.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6759-6772"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

REverse transcriptase ACTivity (REACT) assay for point-of-care measurement of established and emerging antiretrovirals for HIV treatment and prevention. 逆转录酶活性（REACT）测定法，用于对用于艾滋病治疗和预防的成熟和新兴抗逆转录病毒药物进行床旁测量。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-28 DOI: 10.1007/s00216-024-05602-4

Cara Brainerd, Maya A Singh, John Tatka, Cosette Craig, Shane Gilligan-Steinberg, Nuttada Panpradist, Megan M Chang, Barry Lutz, Ayokunle O Olanrewaju

{"title":"REverse transcriptase ACTivity (REACT) assay for point-of-care measurement of established and emerging antiretrovirals for HIV treatment and prevention.","authors":"Cara Brainerd, Maya A Singh, John Tatka, Cosette Craig, Shane Gilligan-Steinberg, Nuttada Panpradist, Megan M Chang, Barry Lutz, Ayokunle O Olanrewaju","doi":"10.1007/s00216-024-05602-4","DOIUrl":"10.1007/s00216-024-05602-4","url":null,"abstract":"Maintaining adequate levels of antiretroviral (ARV) medications is crucial for the efficacy of HIV treatment and prevention regimens. Monitoring ARV levels can predict or prevent adverse health outcomes like treatment failure or drug resistance. However, conventional ARV measurement using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is slow, expensive, and centralized delaying clinical and behavioral interventions. We previously developed a rapid enzymatic assay for measuring nucleotide reverse transcriptase inhibitors (NRTIs) - the backbone of HIV treatment and prevention regimens - based on the drugs' termination of DNA synthesis by HIV reverse transcriptase (RT) enzyme. Here, we expand our work to include non-nucleoside reverse transcriptase inhibitors (NNRTIs) - an ARV class used in established and emerging HIV treatment and prevention regimens. We demonstrate that the REverse Transcriptase ACTivity (REACT) assay can detect NNRTIs including medications used in oral and long-acting/extended-release HIV treatment and prevention. We demonstrate that REACT can measure NNRTIs spiked in either buffer or diluted plasma and that fluorescence can be measured on both a traditional plate reader and an inexpensive portable reader that can be deployed in point-of-care (POC) settings. REACT measured clinically relevant concentrations of five NNRTIs spiked in aqueous buffer. REACT measurements showed excellent agreement between the plate reader and the portable reader, with a high correlation in both aqueous buffer (Pearson's r = 0.9807, P < 0.0001) and diluted plasma (Pearson's r = 0.9681, P < 0.0001). REACT has the potential to provide rapid measurement of NNRTIs in POC settings and may help to improve HIV treatment and prevention outcomes.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"6809-6818"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of secreted signaling molecule sensing on cell monitoring platforms: a critical review. 细胞监测平台上分泌信号分子传感的整合：重要综述。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-07-24 DOI: 10.1007/s00216-024-05435-1

Enrique Azuaje-Hualde, Juncal A Alonso-Cabrera, Marian M de Pancorbo, Fernando Benito-Lopez, Lourdes Basabe-Desmonts

{"title":"Integration of secreted signaling molecule sensing on cell monitoring platforms: a critical review.","authors":"Enrique Azuaje-Hualde, Juncal A Alonso-Cabrera, Marian M de Pancorbo, Fernando Benito-Lopez, Lourdes Basabe-Desmonts","doi":"10.1007/s00216-024-05435-1","DOIUrl":"10.1007/s00216-024-05435-1","url":null,"abstract":"Monitoring cell secretion in complex microenvironments is crucial for understanding cellular behavior and advancing physiological and pathological research. While traditional cell culture methods, including organoids and spheroids, provide valuable models, real-time monitoring of cell secretion of signaling molecules remains challenging. Integrating advanced monitoring technologies into these systems often disrupts the delicate balance of the microenvironment, making it difficult to achieve sensitivity and specificity. This review explored recent strategies for integrating the monitoring of cell secretion of signaling molecules, crucial for understanding and replicating cell microenvironments, within cell culture platforms, addressing challenges such as non-adherent cell models and the focus on single-cell methodologies. We highlight advancements in biosensors, microfluidics, and three-dimensional culture methods, and discuss their potential to enhance real-time, multiplexed cell monitoring. By examining the advantages, limitations, and future prospects of these technologies, we aim to contribute to the development of integrated systems that facilitate comprehensive cell monitoring, ultimately advancing biological research and pharmaceutical development.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7249-7266"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of divalent camelid single-domain antibody and its application in immunoassays for Salmonella detection in food. 二价骆驼单域抗体的制备及其在食品中沙门氏菌检测免疫测定中的应用

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-26 DOI: 10.1007/s00216-024-05610-4

Yanhong Chen, Yingjie Huang, Ruo Yang, Yongliang Cui, Yanru Wang, Xiaohong Wang, Jia Wang

{"title":"Preparation of divalent camelid single-domain antibody and its application in immunoassays for Salmonella detection in food.","authors":"Yanhong Chen, Yingjie Huang, Ruo Yang, Yongliang Cui, Yanru Wang, Xiaohong Wang, Jia Wang","doi":"10.1007/s00216-024-05610-4","DOIUrl":"10.1007/s00216-024-05610-4","url":null,"abstract":"Salmonella-related foodborne infections are commonly caused by the serovars of S. Typhimurium, which can be detected using antibody-based immunoassays. The monovalent variable domain of the camelid heavy chain antibody (VHH) performs excellently in constructing multivalent VHH variants, which generally exhibit higher affinities with antigens and consequently enhance the assay sensitivity. In this study, the divalent variants of VHHs (diVHHs) targeting S. Typhimurium were generated by encoding the monovalent VHH genes assembled in tandem with a flexible linker peptide (G4S)2. Soluble diVHHs were produced in a prokaryotic expression system and purified with a yield of 4.22 mg/L. Benefiting from their stability and antigen-binding abilities towards tested Salmonella serovars, diVHH-based immunoassays were developed. The diVHH-based sandwich immunoassay, using diVHH as capture antibody, exhibited a detection limit of 1.04×102 CFU/mL and enabled as low as 10 CFU/mL S. Typhimurium to be detected after 6 h of enrichment in lettuce. Furthermore, this assay can be applied to spiked lettuce, chicken, and pork samples, showing acceptable recoveries ranging from 83 to 106%. This study presented feasible strategies for VHH multivalence and established a superior sensitivity VHH-based immunoassay for monitoring and analyzing Salmonella contamination in food.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7063-7072"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Membrane-based preparation for mass spectrometry imaging of cultures of bacteria. 基于膜的细菌培养物质谱成像制备方法。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1007/s00216-024-05622-0

Farès Slimani, Laurence Hotel, Aurélie Deveau, Bertrand Aigle, Patrick Chaimbault, Vincent Carré

{"title":"Membrane-based preparation for mass spectrometry imaging of cultures of bacteria.","authors":"Farès Slimani, Laurence Hotel, Aurélie Deveau, Bertrand Aigle, Patrick Chaimbault, Vincent Carré","doi":"10.1007/s00216-024-05622-0","DOIUrl":"10.1007/s00216-024-05622-0","url":null,"abstract":"The study of the dialogue between microorganisms at the molecular level is becoming essential to understand their relationship (antagonist, neutral, or beneficial interactions) and its impact on the organization of the microbial community. Mass spectrometry imaging (MSI) with matrix-assisted laser desorption/ionization (MALDI) is a technique that reveals the spatial distribution of molecules on a sample surface that may be involved in interactions between organisms. An experimental limitation to perform MALDI MSI is a flat sample surface, which in many cases could not be achieved for bacterial colonies such as filamentous bacteria (e.g., Streptomyces). In addition, sample heterogeneity affects sample dryness and MALDI matrix deposition prior to MSI. To avoid such problems, we introduce an additional step in the sample preparation. A polymeric membrane is interposed between the microorganisms and the agar-based culture medium, allowing the removal of bacterial colonies prior to MSI of the homogeneous culture medium. A proof of concept was evaluated on Streptomyces ambofaciens (a soil bacterium) cultures on solid media. As the mycelium was removed at the same time as the polymeric membrane, the metabolites released into the medium were spatially resolved by MALDI MSI. In addition, extraction of the recovered mycelium from the membrane confirmed the identification of the metabolites by ESI MS/MS analysis. This approach allows both the spatial distribution of metabolites produced by microorganisms in an agar medium to be studied under well-controlled sample preparation and their structure to be elucidated. This capability is illustrated using desferrioxamine E, a siderophore produced by S. ambofaciens.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"7161-7172"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0