A new wide-scope, multi-biomarker wastewater-based epidemiology analytical method to monitor the health and well-being of inhabitants at a metropolitan scale.

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Harry Elliss, Kit Proctor, Megan Robertson, John Bagnall, Barbara Kasprzyk-Hordern
{"title":"A new wide-scope, multi-biomarker wastewater-based epidemiology analytical method to monitor the health and well-being of inhabitants at a metropolitan scale.","authors":"Harry Elliss, Kit Proctor, Megan Robertson, John Bagnall, Barbara Kasprzyk-Hordern","doi":"10.1007/s00216-025-06097-3","DOIUrl":null,"url":null,"abstract":"<p><p>This manuscript establishes a new, comprehensive biomarker list and a multiresidue trace quantification method for community-wide health and well-being assessment at a metropolitan scale using wastewater-based epidemiology (WBE) and mass spectrometry pipelines. This method enables the quantification of 204 biochemical indicators (BCIs) across a range of biomarker classes within influent wastewater and includes illicit drug BCIs, pharmaceuticals as proxies for disease, health markers (hormones, oxidative stress, lipid peroxidation, etc.), Lifestyle chemicals, food BCIs, and hazardous chemicals in personal care products. This method facilitates the combined assessment of community exposure to chemicals and the effects of this exposure in the same framework. The method enables full quantification of 141 BCIs with method detection Limits varying from 0.01 ng/L for amlodipine to 23.8 ng/L for stachydrine. Total average method accuracies were 102.7% whereas precision was 10.4%. During an initial assessment of this method to test its suitability, 62% of all targets were detected and quantified during a week-long feasibility study of a large city with weekly average Daily BCI loads ranging from 40.0 ± 20.0 mg/day for salbutamol to 5836.5 ± 1697.1 g/day for creatinine. The inclusion of new endogenous markers such as advanced glycation end products, detected in wastewater for the first time, enables more accurate determination of community-level health and lifestyle habits. Alongside an unbiased and comprehensive health assessment through endogenous markers, health is further assessed via the use of pharmaceuticals, acting as a proxy for health and disease status whilst additionally providing insights into community lifestyle habits through the monitoring of licit/illicit drug use and food consumption. The analysis of all biomarker classes combined aims to provide insights to exposure and health effect outcomes at the community level.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Bioanalytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s00216-025-06097-3","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

This manuscript establishes a new, comprehensive biomarker list and a multiresidue trace quantification method for community-wide health and well-being assessment at a metropolitan scale using wastewater-based epidemiology (WBE) and mass spectrometry pipelines. This method enables the quantification of 204 biochemical indicators (BCIs) across a range of biomarker classes within influent wastewater and includes illicit drug BCIs, pharmaceuticals as proxies for disease, health markers (hormones, oxidative stress, lipid peroxidation, etc.), Lifestyle chemicals, food BCIs, and hazardous chemicals in personal care products. This method facilitates the combined assessment of community exposure to chemicals and the effects of this exposure in the same framework. The method enables full quantification of 141 BCIs with method detection Limits varying from 0.01 ng/L for amlodipine to 23.8 ng/L for stachydrine. Total average method accuracies were 102.7% whereas precision was 10.4%. During an initial assessment of this method to test its suitability, 62% of all targets were detected and quantified during a week-long feasibility study of a large city with weekly average Daily BCI loads ranging from 40.0 ± 20.0 mg/day for salbutamol to 5836.5 ± 1697.1 g/day for creatinine. The inclusion of new endogenous markers such as advanced glycation end products, detected in wastewater for the first time, enables more accurate determination of community-level health and lifestyle habits. Alongside an unbiased and comprehensive health assessment through endogenous markers, health is further assessed via the use of pharmaceuticals, acting as a proxy for health and disease status whilst additionally providing insights into community lifestyle habits through the monitoring of licit/illicit drug use and food consumption. The analysis of all biomarker classes combined aims to provide insights to exposure and health effect outcomes at the community level.

一种新的基于废水的大范围、多生物标志物流行病学分析方法,用于监测大都市规模居民的健康和福祉。
本文建立了一种新的、全面的生物标志物清单和多残留痕量定量方法,用于大都市规模的基于废水的流行病学(WBE)和质谱管道的社区健康和福祉评估。该方法可以量化进水废水中一系列生物标记物类别中的204个生化指标(bci),包括非法药物bci、代表疾病的药物、健康标记物(激素、氧化应激、脂质过氧化等)、生活方式化学品、食品bci和个人护理产品中的危险化学品。这种方法有助于在同一框架内对社区接触化学品及其影响进行综合评估。该方法可对141种bci进行充分定量,方法检出限从氨氯地平的0.01 ng/L到水水碱的23.8 ng/L不等。方法总平均准确度为102.7%,精密度为10.4%。在初步评估该方法的适用性时,对某大城市进行了为期一周的可行性研究,检测并量化了62%的目标,每周平均每日BCI负荷范围为沙丁胺醇40.0±20.0 mg/天至肌酐5836.5±1697.1 g/天。首次在废水中检测到的新的内源性标记物,如晚期糖基化终产物,能够更准确地确定社区水平的健康和生活习惯。除了通过内源性标志物进行公正和全面的健康评估外,还通过药物的使用进一步评估健康,作为健康和疾病状况的代理,同时通过监测合法/非法药物使用和食品消费,进一步了解社区生活习惯。对所有生物标志物类别的综合分析旨在提供对社区水平的暴露和健康影响结果的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信