Development of smartphone-based AIE fluorescence-quenching immunochromatographic sensors for the detection of illicit drugs in various complex sample matrices.
Lijiao Liu, Guanbo He, Lifang Ye, Yong He, Tao Xu, Xiaoli Zhang, Chenxing Zeng, Qifang Song, Jiajie Liang, Yong Tang
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引用次数: 0
Abstract
Illicit drug abuse poses a significant global threat to public health and social security, highlighting the urgent need for rapid, sensitive, and versatile detection technologies. To address the limitations of traditional chromatographic techniques-such as high costs and slow response times-and the drawbacks of conventional immunochromatographic sensors (ICS), including low sensitivity and non-intuitive signal outputs, a fluorescence-quenching ICS (FQICS) was developed. This sensor leverages fluorescence resonance energy transfer (FRET) between aggregation-induced emission fluorescent microspheres (AIEFMs) and gold nanoparticles (AuNPs). The ICS operates on a positive signal-readout mechanism and is integrated with a smartphone-based portable reader, enabling rapid quantitative detection of methamphetamine (MET), morphine (MOR), and ketamine (KET). Detection limits of 0.041, 0.072, and 0.059 ng mL-1 were determined for MET, MOR, and KET, respectively. Recovery rates ranged from 73% to 134% across urine, hair, saliva, and sewage samples, with intra-assay precision consistently below 15%, indicating robust performance in complex matrices. Furthermore, we developed a multiplexed AIEFM-FQICS, enabling the simultaneous detection of three illicit drugs, thereby enhancing detection efficiency and reducing the cost. Generally, this work presents a highly sensitive, field-deployable platform for real-time monitoring in drug interdiction and public safety emergencies, offering substantial potential for practical anti-drug applications.
非法药物滥用对公共卫生和社会安全构成重大全球威胁,突出表明迫切需要快速、敏感和通用的检测技术。为了解决传统色谱技术的局限性(如高成本和慢响应时间)和传统免疫层析传感器(ICS)的缺点(包括低灵敏度和不直观的信号输出),开发了荧光猝灭ICS (FQICS)。该传感器利用荧光共振能量转移(FRET)之间的聚集诱导发射荧光微球(aiefm)和金纳米颗粒(AuNPs)。ICS采用正面信号读出机制,并与基于智能手机的便携式读取器集成,能够快速定量检测甲基苯丙胺(MET)、吗啡(MOR)和氯胺酮(KET)。MET、MOR和KET的检出限分别为0.041、0.072和0.059 ng mL-1。尿液、毛发、唾液和污水样品的回收率从73%到134%不等,测定内精度始终低于15%,表明在复杂基质中具有良好的性能。此外,我们开发了一个多路AIEFM-FQICS,可以同时检测三种非法药物,从而提高了检测效率并降低了成本。总的来说,这项工作提供了一个高度敏感的、可现场部署的平台,用于禁毒和公共安全紧急情况的实时监测,为实际禁毒应用提供了巨大的潜力。
期刊介绍:
Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.