Analytical and Bioanalytical Chemistry最新文献

{"title":"Direct transfer of multicellular tumor spheroids grown in agarose microarrays for high-throughput mass spectrometry imaging analysis.","authors":"Yuan Liu, Jillian Johnson, Hua Zhang, Penghsuan Huang, Lingjun Li","doi":"10.1007/s00216-025-05843-x","DOIUrl":"https://doi.org/10.1007/s00216-025-05843-x","url":null,"abstract":"<p><p>Multicellular tumor spheroids (MCTSs) play an important role in biological studies and cancer research. There is an emerging research interest in molecular profiling and drug distribution of MCTSs by leveraging the superior sensitivity and molecular specificity of mass spectrometry imaging (MSI). Current methods for sample preparation of MCTSs can suffer from low throughput, as MCTSs are typically individually transferred from cell culture into an MSI embedding media and sectioned individually, or sometimes, a few spheroids are placed in a small block of embedding media in preparation for MSI. Here, we developed a method to minimize the sample preparation steps needed to create high-throughput MCTS frozen sections for MSI. Agarose-based microarrays created from Microtissues^® molds were used during MCTS culturing, after which the entire MCTS agarose microarray was taken out of the cell culture well and then directly embedded in 5% gelatin, without the need for a transfer step for each individual MCTS into the embedding media. This method enables rapid profiling of up to 81 MCTSs for larger MCTSs (500-800 µm) or up to 256 MCTSs for smaller MCTSs (200-300 µm) in a single section, remarkably improving the throughput possible for MSI MCTS workflows. Notably, sectioning MCTSs together in the agarose microarray also improves MCTS visualization during sectioning, such that staining each MCTS section to ensure the presence of the MCTSs within the embedding media is not necessary during the sectioning process. The method described here provides a more direct, convenient strategy to achieve high-throughput sections. MSI MCTS sectioning throughput is an important advancement for both pharmaceutical testing of MCTS; the direct transfer 3D cell cultures grown within cell culture-compatible polymer scaffolding are also critical for expanding MSI for the characterization of microfluidic and complex in vitro models, where agarose is readily utilized as a non-adhesive 3D cell culture scaffold.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of microprefilter for chromatography column in HbA1c assays.","authors":"Zhiyan Li, Hongying Wang, Le Chang, Cunling Yan","doi":"10.1007/s00216-025-05844-w","DOIUrl":"https://doi.org/10.1007/s00216-025-05844-w","url":null,"abstract":"<p><p>The complexity of blood and its matrix in high-performance liquid chromatography (HPLC) glycated hemoglobin (HbA1c) assays is closely related to column performance. However, the available prefilters with a single structure or surface membrane materials are not ideal for column protection, and coupled with the complexity of blood samples, leads to rapid degradation of column performance. Therefore, we have developed a new microprefilter with a three-stage filtration design and depth filter material to protect the column. All filter materials used in the preparation of microprefilters were characterized, screened, and optimized, and then manufactured on the basis of optimized filter materials, which are depth filter material microprefilters. Based on the material and structural design, microprefilters were capable of filtering particulate matter from test samples on a step-by-step basis to avoid the plugging effect that occurs when all sizes of substances are gathered together. Moreover, all newly developed microprefilters can be tested more times, up to 600 times. Microprefilters with small-pore-size final filtration membranes of polyethersulfone, hydrophilic polytetrafluoroethylene, and mixed cellulose showed excellent column protection in terms of column efficiency, HbA1c retention time, number of column tests, and column backpressure, and prolonged column lifetime by as much as 20-30% compared with microprefilters with large-pore-size final membranes. Our study provides valuable depth filter material microprefilters with multistage filtration for chromatography columns, and showed excellent column protection and prolonged column lifetime. Meanwhile, microprefilters can be tested more times. The newly developed microprefilters with a small-pore-size final membrane are the optimal choice for column protection of the HbA1c assay.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pressurized fluid extraction of bioactive compounds from peanut by-products to promote waste recovery and circular economy.","authors":"Clara Schumann, Beatriz Martín-Gómez, Ana Jano, Ana M Ares, José Bernal","doi":"10.1007/s00216-025-05839-7","DOIUrl":"https://doi.org/10.1007/s00216-025-05839-7","url":null,"abstract":"<p><p>This work is based on the development and optimization of a pressurized liquid extraction method to obtain extracts from peanut shells with the highest possible amount/number of bioactive compounds, mainly flavonoids, with senolytic activity and antioxidant capacity. To achieve optimal extraction conditions, a design of experiments approach was employed to perform a limited and relatively reduced number of experiments. The extracts were consecutively analyzed by methods adapted to the peanut shell matrix to determine antioxidant capacity, total flavonoids, and total phenolic compounds. Additionally, a high-performance liquid chromatography coupled with diode array detection method was developed and validated to quantify individual phenolic compounds, with confirmation provided by mass spectrometry. Moreover, amino acid profiling was performed using gas chromatography coupled with mass spectrometry. Finally, the optimized extraction conditions and analytical methods were applied to analyze six commercial peanut shell samples. The results indicate that the optimized pressurized liquid extraction method using ethanol effectively extracts substantial amounts of bioactive compounds, especially flavonoids, which have broad applications across different industries. This contributes to a strategic valorization approach that promotes a Circular Economy.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a multiclass LC-MS/MS method for the analysis of cyanotoxins.","authors":"Lydia Zamlynny, Hannah M Morris, Sabrina D Giddings, Johannes Kollatz, Timo H J Niedermeyer, Rob C Jamieson, Daniel G Beach","doi":"10.1007/s00216-025-05829-9","DOIUrl":"https://doi.org/10.1007/s00216-025-05829-9","url":null,"abstract":"<p><p>Cyanobacteria are prokaryotic organisms that can form large monospecific blooms, which pose a risk to human and animal health as some species produce toxic secondary metabolites called cyanotoxins. Multiclass cyanotoxin analysis is challenging due to varying chemical and physical properties between classes, as well as potentially large numbers of analogues within each class. Incorporating anatoxins (ATXs) into multiclass methods can be particularly challenging due to their small molecular size, potential interferences, polarity, and a lack of chemical standards for most analogues. Here, we present the development of a multiclass LC-MS/MS method and a quantitative calibration solution for aetokthonotoxin (AETX), an emerging cyanotoxin linked to mass mortalities of bald eagles in the Eastern United States. The developed method is capable of detecting 17 microcystins (MCs), nodularin-R, three cylindrospermopsins (CYNs), AETX, and 17 ATXs, including recently tentatively identified 10-hydroxy analogues. Analytes were identified by retention time and product ion ratio matching with available standards. The method was evaluated with respect to limits of detection (LODs), linear range, accuracy, and precision using neat and matrix matched standards. LODs in wet cyanobacterial biofilms ranged from 0.14 ng/g for CYN to 2.8 ng/g for [Dha⁷]MC-LR with accuracies ranging from 65% for [Leu¹]MC-LY to 116% for CYN. Finally, the method's application was demonstrated through analysis of cyanobacterial field samples, a dietary supplement matrix reference material, and passive sampler extracts to assess versatility within different matrices.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry assay for quantification of methoxy-polyethylene glycol propionic acid polymers.","authors":"Chunpeng Feng, Jiye Tian, Qisheng Fang, Yajie Cheng, Yue Deng, JiaRui Zhang, Shuang Feng, Qingbin Wang, Hecheng Wang, Xuan Zhao, Lei Yin","doi":"10.1007/s00216-025-05849-5","DOIUrl":"https://doi.org/10.1007/s00216-025-05849-5","url":null,"abstract":"<p><p>As a synthetic polymer, methoxy-polyethylene glycol propionic acid (M-PEG-PA) is widely used in the biomedicine field. Unraveling the pharmacokinetic behavior of M-PEG₆-PA in vivo is crucial for evaluating the safety and efficiency of M-PEG-PA-related polymers or drug delivery systems. A high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry (UPC²-MS/MS) assay was firstly developed and validated for the determination of M-PEG₆-PA polymers in a biological matrix. The MRM transition (mass to charge ratio, precursor ions→fragment ions) of m/z 367.2→118.9 was used to quantify M-PEG₆-PA in this study. The throughput of the assay is high and the total running time for each sample was only 2 min. The linear range of the developed UPC²-MS/MS assay for quantification of M-PEG₆-PA in a biological matrix is 0.05 to 30 μg/mL (R≥0.995). Intra-day and inter-day precisions for the determination of M-PEG₆-PA by this analytical assay were <6.99%. The absolute recoveries and matrix effects of M-PEG₆-PA ranged from 79.50 to 92.47% and 68.72 to 81.73%, respectively. The UPC²-MS/MS assay was successfully applied to quantify the concentrations of M-PEG₆-PA polymers in rat plasma samples.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of MALDI TOF and DART mass spectrometry as novel tools for classification of anaerobic gut fungi strains.","authors":"Markus Neurauter, Julia M Vinzelj, Sophia F A Strobl, Christoph Kappacher, Tobias Schlappack, Jovan Badzoka, Sabine M Podmirseg, Christian W Huck, Matthias Rainer","doi":"10.1007/s00216-025-05846-8","DOIUrl":"https://doi.org/10.1007/s00216-025-05846-8","url":null,"abstract":"<p><p>Anaerobic gut fungi (AGF) have emerged as promising candidates for optimized biogas and biofuel production due to their unique repertoire of potent lignocellulose-degrading enzymes. However, identifying AGF strains through standard fungal DNA barcodes still poses challenges due to their distinct genomic features. This study explored the applicability of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI) and direct analysis in real-time (DART) mass spectrometry (MS) as alternative methods for AGF identification. Further, the capability of the methods to differentiate strains from different growth phases was investigated. The study found that both MALDI and DART were viable methods for AGF strain identification. MALDI proved to be a precise and robust technique for strain discrimination with prediction accuracies of 94% for unknown standard samples. Even at longer growth times (>3 weeks) MALDI achieved good prediction accuracies with 84%; however, younger cultures (72 h) were only predicted with 63% accuracy. The fast on-target lysis with minimal chemical demand yielded suitable spectra for strain differentiation. DART MS, while effective with prediction accuracies of samples with the same age of up to 93%, exhibited lower prediction accuracies for cultures of different ages, with 14% for young (72 h) and 71% for old (>3 weeks) samples. Further research could enhance the capabilities of these mass spectrometry methods for AGF identification and broaden their application to species-level discrimination and a wider range of AGF genera.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous enantioselective determination of 2-, 3-, and 4-methylmethcathinones; their isomers; and major phase-1 metabolites in oral fluid of drug abusers using enantioselective high-performance liquid chromatography-tandem mass spectrometry.","authors":"Giorgi Kobidze, Alfredo Fabrizio Lo Faro, Aurora Balloni, Giorgia Sprega, Marta Massano, Sarah M R Wille, Giuseppe Basile, Tivadar Farkas, Anastasio Tini, Francesco Paolo Busardò, Bezhan Chankvetadze","doi":"10.1007/s00216-025-05838-8","DOIUrl":"https://doi.org/10.1007/s00216-025-05838-8","url":null,"abstract":"<p><p>The most powerful and widely used detector for clinical and toxicological analyses is undoubtedly the mass spectrometer (MS) due to its specificity and high sensitivity. However, it cannot differentiate enantiomers from each other, as well as cannot easily distinguish between positional isomers. Therefore, the components of interest to the analysis at hand need to be separated prior to their detection by MS in order to reliably identify and quantify their enantiomers and positional isomers. In the present study, the simultaneous chemo- and enantioseparation of the drugs of abuse, 2-, 3-, and 4-methylmethcathinones, is described. The developed method was applied to 15 oral fluid (OF) samples collected by police in Belgium and found positive for mephedrone (4-methylmethcathinone, 4-MMC) based on a nonselective method for enantiomers and positional isomers. However, re-analyzing these samples with the analytical method proposed in this report indicated that mephedrone was present in only 1 of them, while 6 samples contained 3-methylmethcathinone (3-MMC) and 12 samples contained 2-methylmethcathinone (2-MMC). At the same time, four OF samples contained both 2- and 3-MMC. The developed method enabled the enantioselective analysis of major metabolites of methylmethcathinones, such as their N-demethyl derivatives (nor-MMCs), as well as, at least partially, of their dihydrometabolites. In addition to their positional isomers, other structural isomers of MMCs, such as buphedrone, metamfepramone, and ethcathinone, could also be detected enantioselectively.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid screening of α-amylase inhibitors from Aloe vera based on polydopamine/L-cysteine bifunctionalized magnetic mesoporous silica immobilized α-amylase.","authors":"Lei Wang, Yue Li, Sikai Wang, Lin Lv, Hongmei Liu, Guoqi Zhang, Yan Zhao","doi":"10.1007/s00216-025-05841-z","DOIUrl":"https://doi.org/10.1007/s00216-025-05841-z","url":null,"abstract":"<p><p>Diabetes mellitus is a metabolic disorder that impacts millions of individuals globally. In the treatment of this condition, it is imperative to explore natural resources for therapeutic agents that exhibit fewer adverse effects and enhanced efficacy. Currently, the methods employed for isolating anti-diabetic lead compounds from natural sources are often intricate and time-consuming. Therefore, there is an urgent need to develop efficient and rapid screening techniques. In this study, α-amylase was immobilized using a novel polydopamine/L-cysteine bifunctionalized magnetic mesoporous silica composite material (Fe₃O₄@nSiO₂@mSiO₂@PDA@L-Cys) for the first time. A ligand fishing approach utilizing the immobilized α-amylase was developed to rapidly screen for α-amylase inhibitors from Aloe vera. Characterization and property analysis of the immobilized enzyme showed that the immobilized α-amylase exhibited exceptional stability and reusability. Two ligands were successfully screened from Aloe vera and then characterized as aloin B and aloin A using ultra-high performance liquid chromatography tandem mass spectrometry. Their respective IC₅₀ values were 0.99 ± 0.09 mM and 1.14 ± 0.05 mM. Molecular docking studies confirmed the interaction of both ligands with specific amino acid residues within the active site of α-amylase. The study presents a fast and efficient approach for screening α-amylase inhibitors from intricate natural sources, thereby offering significant potential for the development of anti-diabetic agents.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface plasmon resonance biosensor for environmental detection of tramadol.","authors":"Magdalena Čapková, Erika Hemmerová, Jiří Homola","doi":"10.1007/s00216-025-05832-0","DOIUrl":"https://doi.org/10.1007/s00216-025-05832-0","url":null,"abstract":"<p><p>Contamination of surface water and drinking water with pharmaceuticals presents an environmental concern. It has been shown to affect aquatic organisms and have adverse health effects on humans. One of the most common pharmaceutical contaminants is the opioid analgesic tramadol. In this communication, we report on the first surface plasmon resonance biosensor-based detection of tramadol in water. The biosensor utilizes a binding inhibition format and enables detection of tramadol at a wide range of concentrations (5 orders of magnitude) with a limit of detection of 0.52 µg/L. The results of a small-scale environmental study are reported in which the biosensor was used to analyze river water samples. The results were found to agree well with those obtained using the liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering DNA circuit powered by entropy integrated into robust and elegant photoelectrochemical and photothermal dual-mode biosensing.","authors":"Jing Li, Bingtao Hu, Yanxin Zhang, Qin Xu, Hongbo Li","doi":"10.1007/s00216-025-05848-6","DOIUrl":"https://doi.org/10.1007/s00216-025-05848-6","url":null,"abstract":"<p><p>Dual-signal mode sensors that can self-validate detection results have attracted considerable interest; however, creating those with superior overall performance still presents significant challenges. Herein, we develop a unique photoelectrochemical (PEC) and photothermal (PT) dual-mode biosensor targeting microRNA-221 (miRNA-221), built on an innovative entropy-driven DNA circuit (EDC). The zinc oxide nanorods (ZnO NRs) serve as PEC beacons, while copper sulfide nanoparticles (CuS NPs) function as photocurrent inhibitors and PT beacons, both biofunctionalized with DNAs before being assembled through partial base pairing. When target miRNA-221 is present, the EDC activates and releases output DNAs that open partially hybridized strands anchored to ZnO NRs via competitive assembly. This process liberates CuS-DNA1 and restores the suppressed photocurrent. The results demonstrate linear relationships between photocurrent/temperature increment and the logarithm of target concentration across ranges of 1.0 fmol L^-1-50.0 pmol L^-1 (limit of detection (LOD): 0.35 fmol L^-1) and 5.0×10² fmol L^-1-5.0 nmol L^-1 (LOD: 1.22×10² fmol L^-1), respectively. Compared to conventional EDCs, our optimally designed EDC not only doubles the output DNA yield but also significantly enhances sensor sensitivity. Additionally, the target-triggered EDC amplification strategy effectively minimizes reversibility in each reaction step, preserves base sequence integrity, boosts efficiency, and demonstrates strong thermal stability and selectivity, thereby increasing the specificity of the dual-mode biosensor. Furthermore, ZnO NR photoelectric beacons fabricated via electrodeposition greatly improve the stability and controllability of the photoelectrode while avoiding lengthy modification processes. Overall, this thoughtfully engineered dual-mode biosensor offers numerous advantages, including a wide linear range, excellent stability, high reproducibility, and user-friendly operation. Specifically, this signal-on type dual-signal output biosensor enables self-confirmation of detection results, significantly enhancing both accuracy and reliability.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}