Analytical and Bioanalytical Chemistry最新文献_第6页

Automated and online LC-MS/MS for multiplexed monitoring of hormone secretion from murine islets. 用于小鼠胰岛激素分泌的自动在线LC-MS/MS多路监测。

IF 4.1 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2026-09-21 DOI: 10.1007/s00216-026-06818-2

Joshua J Davis, James Thornham, Michael G Roper

{"title":"Automated and online LC-MS/MS for multiplexed monitoring of hormone secretion from murine islets.","authors":"Joshua J Davis, James Thornham, Michael G Roper","doi":"10.1007/s00216-026-06818-2","DOIUrl":"10.1007/s00216-026-06818-2","url":null,"abstract":"Islets of Langerhans are the endocrine microorgans responsible for secretion of hormone peptides to regulate blood glucose. Five endocrine cell types make up islets, each releasing specific peptide hormone(s) in response to extra- and intra-islet signals. Secretory dysfunction is associated with several metabolic diseases; development of analytical technologies that can temporally resolve multiple secreted factors would therefore aid in understanding factors that govern healthy and disrupted secretion from these cells. To this end, an online liquid chromatography with tandem mass spectrometry (LC-MS/MS) approach that had been developed for analysis of hormone secretion from human islets of Langerhans was modified to be used with murine islets. Rodent islets provide easily accessible samples for many researchers and mimic the biology of human islets; however, there have been no published reports on the development of analytical approaches for measuring multi-hormone samples from these species. Online perfusion of ~ 30 murine islets was performed in a microfluidic system with the perfusate coupled to a 1.5 min LC separation via a six-port valve. The valve was automated to achieve a ~ 2 min sampling period from the perfusate, providing temporally resolved measurements of somatostatin, glucagon, insulin I, and insulin II. The triple quadrupole MS provided < 0.5 nM limits of quantitation for each peptide. Secretion from murine islets in response to elevated glucose was performed, as well as more complex sinusoidal glucose levels. The automation, quantitation, and ability to identify multiple hormone species simultaneously is expected to provide new insight into the factors that govern hormone secretion from islets.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymer-stabilized zinc sulfide colloids as a candidate calibration material for sulfide determination in water. 聚合物稳定的硫化锌胶体作为水中硫化物测定的候选校准材料。

IF 4.1 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2026-09-21 DOI: 10.1007/s00216-026-06820-8

Peng Li, Hongkun Yu, Kunde Lin

{"title":"Polymer-stabilized zinc sulfide colloids as a candidate calibration material for sulfide determination in water.","authors":"Peng Li, Hongkun Yu, Kunde Lin","doi":"10.1007/s00216-026-06820-8","DOIUrl":"https://doi.org/10.1007/s00216-026-06820-8","url":null,"abstract":"Accurate quantification of sulfide in water depends critically on reliable calibration materials. However, existing calibration materials pose significant challenges for routine sulfide analysis, whether they are in the form of Na2S solutions susceptible to atmospheric oxidation or ZnS suspensions lacking physical homogeneity. To address these deficiencies, we developed a series of highly stable ZnS colloids by using zinc acetate, sodium sulfide, and a polymeric stabilizer. Four polymers were demonstrated to be effective, namely, chitosan, hydroxyethyl starch, hydroxyethyl cellulose, and hydroxypropylmethyl cellulose. Under optimized experimental conditions, each ZnS colloid formulation maintained satisfactory homogeneity and stability for over 6 months. Moreover, the colloids exhibit no spectral interference with the widely adopted methylene blue spectrophotometry at around 660 nm and do not cause clogging of online analyzer tubing, suggesting their suitability for instrument self-calibration. Particle size and zeta potential analyses revealed that adding a stabilizer effectively prevented particle aggregation, consistent with predominantly steric hindrance imparted by the adsorbed polymer layers. Collectively, the proposed ZnS colloids represent a promising alternative to conventional sulfide calibration materials for method development, instrument calibration, and data quality control.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Programmable hairpin assembly circuit-cascaded DNAzyme amplification for ultrasensitive miRNA-146a detection via high-stable perovskite nanocomposite biosensor. 可编程发夹组装电路级联DNAzyme扩增用于高稳定钙钛矿纳米复合生物传感器的超灵敏miRNA-146a检测。

IF 4.1 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2026-09-20 DOI: 10.1007/s00216-026-06792-9

Xiaohong Liu, Dandan Xu, Luna Guo, Weiling Song, Hong Zhou, Deshan Liu

{"title":"Programmable hairpin assembly circuit-cascaded DNAzyme amplification for ultrasensitive miRNA-146a detection via high-stable perovskite nanocomposite biosensor.","authors":"Xiaohong Liu, Dandan Xu, Luna Guo, Weiling Song, Hong Zhou, Deshan Liu","doi":"10.1007/s00216-026-06792-9","DOIUrl":"https://doi.org/10.1007/s00216-026-06792-9","url":null,"abstract":"The development of accurate and sensitive analytical methods for tumor-associated microRNAs (miRNAs) is of great practical significance for cancer monitoring. Herein, a novel fluorescent biosensor platform for miR-146a was constructed by combining a CsPbBr3@MSN fluorescent probe with strand triggered hairpin assembly amplification (SHA)-driven catalysis-DNAzyme cascade amplification technology. The fluorescent probe was prepared by embedding the CsPbBr3 quantum dots into mesoporous silica nanomaterials (MSN), which provided the stable and strong fluorescence properties, as well as good water solubility. In the presence of miR-146a, SHA was initiated, subsequently activating the DNAzyme to produce fluorescence. The amplification process in this assay was enzyme-free and only requires magnetic separation, thereby reducing the experimental cost. As a result, the detection limit of miR-146a reached 3.78 fM, with a linear range of 5 fM-50 nM. In addition, the developed fluoresence sensor demonstrated outstanding selectivity, reproducibility, stability, and recoveries (96.93-112%) in real sample analysis, making it promising for biological research and early disease diagnosis.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A portable multimodal electrochemical platform integrating β-cyclodextrin and Pd-Au@ZnO nanostructures for the chiral recognition of valine enantiomers in complex biological matrices. 结合β-环糊精和Pd-Au@ZnO纳米结构的便携式多模态电化学平台，用于手性识别复杂生物基质中的缬氨酸对映体。

IF 4.1 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2026-09-19 DOI: 10.1007/s00216-026-06803-9

Ramona Georgescu-State, Ionela Raluca Comnea-Stancu, Razvan Nicolae State, Florica Papa, Jacobus Koos Frederick van Staden, Raluca-Ioana Stefan-van Staden

{"title":"A portable multimodal electrochemical platform integrating β-cyclodextrin and Pd-Au@ZnO nanostructures for the chiral recognition of valine enantiomers in complex biological matrices.","authors":"Ramona Georgescu-State, Ionela Raluca Comnea-Stancu, Razvan Nicolae State, Florica Papa, Jacobus Koos Frederick van Staden, Raluca-Ioana Stefan-van Staden","doi":"10.1007/s00216-026-06803-9","DOIUrl":"https://doi.org/10.1007/s00216-026-06803-9","url":null,"abstract":"The development of a straightforward and efficient multimodal electrochemical platform for enantiomeric discrimination is of considerable importance in clinical diagnostics and life sciences. In this study, a compact chiral electrochemical platform was fabricated by modifying a screen-printed carbon nanofiber electrode with β-cyclodextrin and zinc oxide nanostructures decorated with bimetallic Pd-Au nanoparticles, designed for the selective recognition and quantification of valine enantiomers in whole blood samples. The platform integrates two operational modes, linear sweep voltammetry and stochastic sensing, allowing both qualitative and quantitative analysis of the target analyte. A comprehensive characterization of the modified electrode was conducted using scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The platform exhibited high enantioselectivity. A well-defined linear relationship was observed between the anodic peak current and the logarithm of valine enantiomer concentrations in the range of 1.0 × 10-8 to 1.0 × 10-4 M. In stochastic mode, a broader linear dynamic range of 1.0 × 10-14-1.0 × 10-6 M was achieved for L-valine and 1.0 × 10-14-1.0 × 10-5 M for D-valine, with quantification limits as low as 1.0 × 10-14 M. The platform demonstrated excellent reproducibility and stable performance over time. High recovery values (99.3-100.9%) were obtained when applied to real blood samples.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149007570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural lipid modifications associated with the persister state in Acinetobacter baumannii: LC-TIMS-TOF for collision cross section determination and focus on uncommon wax esters. 与鲍曼不动杆菌持续状态相关的结构脂质修饰：LC-TIMS-TOF碰撞截面测定和关注罕见蜡酯。

IF 4.1 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2026-09-18 DOI: 10.1007/s00216-026-06824-4

Estelle Deschamps, Delphine Vergoz, Emmanuelle Dé, Carlos Afonso, Stéphane Alexandre, Corinne Loutelier-Bourhis

{"title":"Structural lipid modifications associated with the persister state in Acinetobacter baumannii: LC-TIMS-TOF for collision cross section determination and focus on uncommon wax esters.","authors":"Estelle Deschamps, Delphine Vergoz, Emmanuelle Dé, Carlos Afonso, Stéphane Alexandre, Corinne Loutelier-Bourhis","doi":"10.1007/s00216-026-06824-4","DOIUrl":"https://doi.org/10.1007/s00216-026-06824-4","url":null,"abstract":"The bacterial membrane is the first barrier against environmental variations and is mainly composed of lipids which have a key role in bacterial growth. Studying them is important for a better understanding of bacterial cell physiology. Notably for persister cells, which constitute a bacterial subpopulation able to survive high concentrations of antibiotics. This work complements a preliminary study of Acinetobacter baumannii persister cell lipids. Here, the coupling of liquid chromatography, ion mobility spectrometry, and tandem mass spectrometry (LC-IMS-MS/MS) allowed a more complete and comprehensive characterization of all lipids in A. baumannii antibiotic persister cells. Moreover, experimental collision cross section (CCS) values for all these lipid species were determined, which should help future annotations, in particular for uncommon lipids such as wax esters (WEs) and 1-phosphatidyl-2-acyl-glycerol-3-phosphoethanolamine (PAGPE), which are reported for the first time in this work. We showed that hydroxylation of fatty acyl chains significantly increased CCS values. Finally, we showed that ammonium and ethylamine adducts can be formed for WEs and that precautions must be taken in WE identification. To this end, this work proposes an analytical methodology and associated precautions for lipid annotation in persister cells using three identification criteria: m/z, retention time, and CCS.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electron-buffering effect of Ce³⁺ enhances copper nanocluster nanozyme activity for cascade-amplified biosensing and portable detection of α-glucosidase. Ce3+的电子缓冲效应增强了铜纳米簇纳米酶的活性，用于级联扩增生物传感和α-葡萄糖苷酶的便携式检测。

IF 4.1 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2026-09-18 DOI: 10.1007/s00216-026-06827-1

Jianjun Kang, JiangRui Guo, Fang Ke, Gengneng Lai, Zongcheng Shu, Xiuzhi Xu, Wei Li

{"title":"Electron-buffering effect of Ce3+ enhances copper nanocluster nanozyme activity for cascade-amplified biosensing and portable detection of α-glucosidase.","authors":"Jianjun Kang, JiangRui Guo, Fang Ke, Gengneng Lai, Zongcheng Shu, Xiuzhi Xu, Wei Li","doi":"10.1007/s00216-026-06827-1","DOIUrl":"https://doi.org/10.1007/s00216-026-06827-1","url":null,"abstract":"Accurate quantification of enzyme activity is essential for early metabolic disease diagnosis and point-of-care bioanalysis. Abnormal α-glucosidase (α-GAA) activity is closely related to metabolic disorders, making its sensitive detection increasingly important. However, most current assays rely on one-to-one signal transduction, where each enzymatic event produces only a single detectable signal, inherently limiting sensitivity and compromising reliable detection under complex physiological conditions. Here, we establish a hierarchical catalytic amplification strategy for ultrasensitive α-GAA detection by integrating enzymatic hydrolysis, glucose oxidase-mediated H2O2 generation, and Ce3+-protected copper nanozyme catalysis. Crucially, a matrix-matched blank subtraction strategy is introduced to effectively eliminate interference from endogenous glucose and other non-specific reductants, ensuring that the final amplified signal exclusively originates from α-GAA activity. Benefiting from this three-stage amplification architecture and the blank correction, the platform enabled highly sensitive α-GAA quantification. Benefiting from this three-stage amplification architecture, the platform enabled highly sensitive α-GAA quantification over a linear range of 0.1-40 U/L with a detection limit of 0.026 U/L. The biosensor further exhibited excellent selectivity, reliable analytical performance in human serum, and portable quantitative analysis through smartphone-assisted colorimetric detection. This work advances nanozyme-based biosensing from conventional signal reporting toward amplification-guided analytical design, providing a generalizable strategy for developing next-generation decentralized diagnostic platforms.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149000998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solution to a stereo-chemically confusing challenge 立体化学难题的解决方案

IF 4.1 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2026-09-17 DOI: 10.1007/s00216-026-06731-8

Reinhard Meusinger

引用次数: 0

A pomander challenge 一个挑战

IF 4.1 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2026-09-17 DOI: 10.1007/s00216-026-06730-9

Reinhard Meusinger

引用次数: 0

Development and characterization of tetrodotoxin reference materials. 河豚毒素标准物质的研制与表征。

IF 4.1 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2026-09-17 DOI: 10.1007/s00216-026-06773-y

Krista M Thomas, Daniel G Beach, Kelley L Reeves, Ruth A Perez-Calderon, Sheila Crain, Elliott S Kerrin, Ryan Gibbs, Michael Boundy, Paul McNabb, D Tim Harwood, Pearse McCarron

{"title":"Development and characterization of tetrodotoxin reference materials.","authors":"Krista M Thomas, Daniel G Beach, Kelley L Reeves, Ruth A Perez-Calderon, Sheila Crain, Elliott S Kerrin, Ryan Gibbs, Michael Boundy, Paul McNabb, D Tim Harwood, Pearse McCarron","doi":"10.1007/s00216-026-06773-y","DOIUrl":"https://doi.org/10.1007/s00216-026-06773-y","url":null,"abstract":"Tetrodotoxin (TTX) is a potent natural toxin commonly associated with puffer fish, originally discovered in Tetraodontidae spp., which primarily inhabit tropical and sub-tropical waters. The more recent detection of TTX in shellfish from temperate waters has increased the demand for analytical capabilities to test for TTX in a variety of sample types. Here, we describe the production of TTX reference materials (RMs) essential for the development, validation, and quality control for TTX analyses. Research to develop a TTX calibration solution considered the impact of solvent, pH, and temperature on stability. The certified reference material (CRM) was prepared in 1 mM acetic acid, value assigned (6.75 ± 0.24 µg/g TTX) using quantitative nuclear magnetic resonance, and was stable at temperatures up to 4 °C over a 2-year stability study. Feasibility studies were performed to produce shellfish matrix RMs by blending mussels (Mytilus edulis) with TTX-contaminated sea slug (Pleurobranchaea maculata). Separate wet tissue and lyophilized materials were evaluated, and good homogeneity was demonstrated for both. In the lyophilized material, TTX was stable at temperatures up to 40 °C over 28 days; however, in the wet homogenate, TTX degraded at temperatures of 4 °C and above over the same time period. Liquid chromatography and capillary electrophoresis methods with tandem mass spectrometry detection were developed for characterization of the materials and showed good agreement. The availability of a TTX calibration solution CRM, established processes for matrix RM preparation, and improved characterization methods provide the tools necessary for supporting TTX analyses in shellfish considering its increased occurrence globally.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging technologies in the spatial proteomics landscape. 空间蛋白质组学领域的新兴技术。

IF 4.1 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2026-09-16 DOI: 10.1007/s00216-026-06822-6

Matthew Heck, Jia Guo

{"title":"Emerging technologies in the spatial proteomics landscape.","authors":"Matthew Heck, Jia Guo","doi":"10.1007/s00216-026-06822-6","DOIUrl":"https://doi.org/10.1007/s00216-026-06822-6","url":null,"abstract":"Spatial proteomics has rapidly advanced the ability to measure protein expression, localization, and tissue organization directly within intact biological specimens. Unlike bulk proteomics or transcript-centered approaches, spatial protein imaging can capture cell-to-cell heterogeneity, local signaling states, and multicellular tissue structure while preserving histologic context. This review examines the major technology classes shaping the field, with primary emphasis on multiplexed in situ protein imaging. We discuss region-of-interest digital spatial profiling, mass spectrometry-based spatial proteomics, iterative immunofluorescence, and signal amplification strategies, and compare their major strengths and limitations in the context of sensitivity, multiplexing capacity, spatial resolution, sample throughput, and tissue compatibility. Particular attention is given to how different signal-reset and amplification designs have expanded fluorescence-based imaging beyond the limits of conventional low-plex immunostaining. We also summarize major biological applications in cancer, immunology, and neurology, where spatial proteomics has enabled the identification of tissue architecture, cellular neighborhoods, and disease-associated protein states that are difficult to resolve by nonspatial methods. Finally, we outline the principal limitations and future directions shaping the field, including incomplete proteome coverage, multimodal spatial integration, image-analysis challenges, assay reproducibility, and translation into standardized biological and clinical workflows. Overall, multiplexed in situ protein imaging is becoming an increasingly important framework for linking molecular state to tissue organization in both biological research and translational pathology.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148981880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0