Medium-entropy nanoenzyme with high oxidase-like activity for sensitive detection of interleukin-6 based on ELISA

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Analytical and Bioanalytical Chemistry Pub Date : 2025-08-26 DOI:10.1007/s00216-025-06054-0

Lifang Chen, Jiaqi Chen, Ying Lei, Pengcheng Lin, Donglin Cao, Wei Xiao, Liangshan Hu

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引用次数: 0

Abstract

Interleukin-6 (IL-6) is an acute phase response protein. Its level rapidly increases in infection, trauma, and acute inflammatory states. It usually appears earlier than other inflammatory markers such as C-reactive protein and procalcitonin. Therefore, detecting abnormal IL-6 levels (The clinical reference range is greater than 7 pg mL⁻¹) faster can help humans receive timely treatment and recover their health. This article successfully synthesized a medium-entropy oxide nanoenzyme. It does not require the involvement of hydrogen peroxide and has a low Michaelis constant of 0.101 mM. Based on the interaction between La-based medium-entropy oxide (MEO-La) nanoenzyme and secondary antibody, specific detection of IL-6 in enzyme-linked immunosorbent assay is achieved. The concentration of IL-6 in human serum can be qualitatively detected. The detection limit and linear range are 0.03 pg mL⁻¹ and 5–1000 pg mL⁻¹, respectively. This work not only is of great significance for better understanding the unique properties of medium-entropy nanoenzymes but also has enormous potential for biochemical sensing of human health.

Graphical Abstract

This work develops a medium entropy oxide nanoenzyme for ultrasensitive IL-6 detection, achieving a detection limit of 0.03 pg mL⁻¹. The hydrogen peroxide free system has shown great potential in early disease diagnosis and biochemical sensing applications.

查看原文本刊更多论文

基于ELISA的高氧化酶样中熵纳米酶灵敏检测白细胞介素-6。

白介素-6 （IL-6）是一种急性期反应蛋白。它的水平在感染、创伤和急性炎症状态下迅速增加。它通常比其他炎症标志物如c反应蛋白和降钙素原出现得更早。因此，更快地检测异常IL-6水平（临床参考范围大于7 pg mL-1）有助于人类及时得到治疗，恢复健康。本文成功合成了一种中熵氧化物纳米酶。它不需要过氧化氢的参与，具有0.101 mM的低米切里斯常数。基于la基中熵氧化物（MEO-La）纳米酶与二抗的相互作用，在酶联免疫吸附法中实现了IL-6的特异性检测。可定性检测人血清中IL-6的浓度。检出限为0.03 pg mL-1，线性范围为5 ~ 1000 pg mL-1。这项工作不仅对更好地了解中熵纳米酶的独特性质具有重要意义，而且对人类健康的生化传感具有巨大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.