Characterization of rare trifucosylated human milk oligosaccharides by cryogenic infrared ion spectroscopy (CIRIS).

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Ali H Abikhodr, Stephan Warnke, Ahmed Ben Faleh, Thomas R Rizzo, Sibel Goeraler, John Gonsalves, Bernd Stahl, Marko Mank
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引用次数: 0

Abstract

While approximately 200 human milk oligosaccharide structures (HMOs) have been completely characterized, the precise isomeric structures of even more HMOs remain ambiguous. To address this analytical challenge, we employ tandem mass spectrometry (i.e., MS/MS) coupled with cryogenic infrared ion spectroscopy (CIRIS) to characterize and identify novel fucosylated HMOs isolated from pooled human milk. In this approach, the precise structure of isomeric precursor molecules purified by liquid chromatography (LC) is identified by IR fingerprinting their fragments. Using this technique, we have identified four novel isomers of trifucosylated oligosaccharides (i.e., trifucosyl-lacto-N-heptaoses and trifucosyl-lacto-N-neoheptaoses), some of which could not have been produced by previously known biosynthetic pathways for HMO synthesis in the mammary gland.

低温红外离子光谱(CIRIS)表征罕见的三聚焦人乳低聚糖。
虽然大约200种人乳寡糖结构(HMOs)已经被完全表征,但更多HMOs的精确异构体结构仍然不明确。为了解决这一分析挑战,我们采用串联质谱(即MS/MS)结合低温红外离子光谱(CIRIS)来表征和鉴定从汇集的人乳中分离的新型聚焦HMOs。该方法利用红外指纹图谱对液相色谱纯化的异构体前体分子进行精确结构鉴定。利用这种技术,我们已经鉴定了四种新的三聚焦寡糖异构体(即三聚焦基-乳酸-n - heptases和三聚焦基-乳酸-n - neoheptases),其中一些不可能通过以前已知的乳腺中HMO合成的生物合成途径产生。
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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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