Analytical and Bioanalytical Chemistry最新文献_第4页

An integrated pH "Pen" for visual nucleic acid detection based on colorimetrical LAMP at point-of-care testing. 一个集成的pH“笔”的视觉核酸检测基于比色灯在护理点检测。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-10-08 DOI: 10.1007/s00216-025-06147-w

Lei Wang, Zhifeng Wu, Jing Niu, Shangzhi Ji, Lulu Zhang, Michael G Mauk, Fang Li, Xianbo Qiu

{"title":"An integrated pH \"Pen\" for visual nucleic acid detection based on colorimetrical LAMP at point-of-care testing.","authors":"Lei Wang, Zhifeng Wu, Jing Niu, Shangzhi Ji, Lulu Zhang, Michael G Mauk, Fang Li, Xianbo Qiu","doi":"10.1007/s00216-025-06147-w","DOIUrl":"https://doi.org/10.1007/s00216-025-06147-w","url":null,"abstract":"<p><p>Nucleic acid detection serves as a critical tool employed for controlling the spread of infectious diseases. However, the majority of current nucleic acid detection methods still rely on large and complex equipment, rendering them unsuitable for point-of-care testing, particularly in remote or resource-limited areas. Therefore, the development of a simple, portable, and visual nucleic acid amplification and detection device is imperative. Based on the pH change during amplification in a non-buffered loop-mediated isothermal amplification (LAMP) system, we proposed an integrated pH \"Pen\" loaded with a pH test strip for endpoint detection of nucleic acid by naked eyes. The pH \"Pen\" was characterized by minimal instrumentation requirements, necessitating only a portable metal block for nucleic acid amplification. The device features a sealed chamber that ensures an accurate colorimetric response by preventing the pH test strip from contacting mineral oil, which is used to minimize reagent evaporation. Additionally, the image analysis method for assisted visual detection mitigates the impact of individual visual differences on color interpretation. By calculating the G/R value of the pH test strip image, this method enables the detection threshold to accommodate different imaging devices and lighting conditions. The non-viral OTU2m gene (a conserved gene derived from the Chlamydomonas reinhardtii strain) was chosen for proof of concept in our study. The \"Pen\" enabled the amplification and detection of the OTU2m gene within 28 min, with a limit of detection as low as 2.0 × 10<sup>2</sup> copies/mL, comparable to the sensitivity of the real-time fluorescence method. Given its simplicity, affordability, portability, and capacity for visual result analysis, the integrated pH \"Pen\" provided a promising method for nucleic acid detection at point-of-care testing, particularly in the condition with limited access to advanced laboratory infrastructure.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From comprehensive profiling to targeted authentication: an FBMN-guided metabolomics strategy for authentication of Pseudostellaria heterophylla Dao-di and non-Dao-di herbs. 从综合分析到目标鉴定：fbmn引导下的杂太子参刀底与非刀底药材代谢组学鉴定策略

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-10-08 DOI: 10.1007/s00216-025-06140-3

Yuxin Ding, Hanbin He, Ye Wang, Chenying Yuan, Xinxin Zheng, Zhen Cao, Yuepiao Cai, Yanchao Xing

{"title":"From comprehensive profiling to targeted authentication: an FBMN-guided metabolomics strategy for authentication of Pseudostellaria heterophylla Dao-di and non-Dao-di herbs.","authors":"Yuxin Ding, Hanbin He, Ye Wang, Chenying Yuan, Xinxin Zheng, Zhen Cao, Yuepiao Cai, Yanchao Xing","doi":"10.1007/s00216-025-06140-3","DOIUrl":"https://doi.org/10.1007/s00216-025-06140-3","url":null,"abstract":"<p><p>Pseudostellaria heterophylla (PH), a high-value medicinal herb with significant economic and clinical importance, faces quality standardization challenges due to wild resource depletion and inconsistent cultivated materials. To lay the foundation for establishing reliable quality standards, we developed an integrated metabolomics platform combining ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) with feature-based molecular networking (FBMN) for phytochemical profiling. FBMN enabled relatively comprehensive metabolite annotation, identifying cohesive clusters of alkaloids/organic acids/purines via conserved fragmentation pathways, while cyclopeptides formed isolated nodes due to structural heterogeneity. Multivariate statistical analysis (PCA, OPLS-DA) and machine learning (K-means clustering) revealed significant chemometric differences between geo-authentic (Fujian) and other major commercial (Anhui, Guizhou, Jiangsu) samples, which were further validated through reference crude drug-controlled intergroup comparative analysis. Notably, six signature metabolites (pseudostellarins A/F, heterophyllin J, ferulic acid, dihydro ferulic acid, and azelaic acid) exhibited markedly higher concentrations in Fujian PH, serving as robust geographical marker candidates. These results lay the foundation supporting Fujian's status as a Dao-di region and establish a systematic framework for geographical traceability. This FBMN-integrated metabolomics approach not only offers a viable strategy for quality control of Dao-di herbs but also proposes a transferable analytical paradigm for geo-authenticity assessment of other herbs.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bimetallic nanotubes enhanced MALDI-TOF MS for rapid detection of carbapenemase activity in Klebsiella pneumoniae. 双金属纳米管增强MALDI-TOF质谱快速检测肺炎克雷伯菌碳青霉烯酶活性。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-10-07 DOI: 10.1007/s00216-025-06135-0

Chenyao Lin, Menglu Mao, Qiaoping Wu, Xuedan Qiu, Dumei Ma, Chuanfan Ding, Yinghua Yan, Qingcao Li

{"title":"Bimetallic nanotubes enhanced MALDI-TOF MS for rapid detection of carbapenemase activity in Klebsiella pneumoniae.","authors":"Chenyao Lin, Menglu Mao, Qiaoping Wu, Xuedan Qiu, Dumei Ma, Chuanfan Ding, Yinghua Yan, Qingcao Li","doi":"10.1007/s00216-025-06135-0","DOIUrl":"https://doi.org/10.1007/s00216-025-06135-0","url":null,"abstract":"<p><p>The global spread of carbapenemase-producing Klebsiella pneumoniae represents a critical threat to public health, necessitating the development of rapid, sensitive, and reliable diagnostic methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has gained attention as a high-throughput, rapid diagnostic tool for detecting carbapenemase-mediated hydrolysis. However, the effectiveness of conventional organic matrices is limited by inadequate stability, low detection sensitivity, and substantial background interference. In this study, we developed novel bimetallic nanotubes (N-CNT@Zn-Ga/MOF@Au) as a MALDI matrix by integrating nitrogen-doped carbon nanotubes (N-CNT) with a Zn-Ga-based metal-organic framework (MOF) and gold nanoparticles (AuNPs). This nanocomposite exhibited a high surface area, enhanced light absorption, and reduced background noise, contributing to improved signal intensity, reproducibility, and analytical sensitivity. Leveraging this matrix, we analyzed 204 K. pneumoniae clinical isolates for carbapenemase activity through imipenem hydrolysis assays. The method demonstrated exceptional performance, achieving 97.2% sensitivity and 100% specificity within 15 min, with KPC-2-producing strains detected with 100% accuracy. Extending incubation to 1 h allowed for reliable detection of all carbapenemase types, including weaker enzymes such as OXA-48. These findings demonstrate the potential of N-CNT@Zn-Ga/MOF@Au as an advanced MALDI matrix for rapid and accurate detection of carbapenemase activity, with important applications in resistance monitoring and clinical decision support.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A critical review on neurodegenerative biomarker diagnostics: where is the field heading to? 神经退行性生物标志物诊断的重要回顾：该领域将走向何方？

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-10-07 DOI: 10.1007/s00216-025-06061-1

Mandana Amiri, Hosein Afshary, Abolfazl Bezaatpour, Sepideh Hatamikia, James Wei, Rabah Boukherroub, Sabine Szunerits

{"title":"A critical review on neurodegenerative biomarker diagnostics: where is the field heading to?","authors":"Mandana Amiri, Hosein Afshary, Abolfazl Bezaatpour, Sepideh Hatamikia, James Wei, Rabah Boukherroub, Sabine Szunerits","doi":"10.1007/s00216-025-06061-1","DOIUrl":"10.1007/s00216-025-06061-1","url":null,"abstract":"<div><p>Neurodegenerative diseases (NDD), a collection of disorders with different underlying causes and clinical presentations, are recognized as a major area of concern of our society today. The most common NDD are Parkinson’s disease (PD), Alzheimer’s disease (AD), amyotrophic lateral sclerosis (ALS), and Huntington’s disease, each one of them being characterized by the progressive degradation of nerve cells and accumulation of misfolded and aggregated proteins in the affected brain region. Diagnosing NDD is challenging, due to the heterogeneity of the disease and the overlap of symptoms. Yet, early detection and accurate diagnosis are crucial for effective NDD management. With the emergence of disease-modifying therapies for AD, monitoring disease progression and treatment success is becoming essential. The future of NND diagnostics is focusing on developing less invasive, cost-effective strategies that enable early NDD identification and detection with improved patient outcomes. The integration of biotechnology and nanotechnology is seen as crucial for advancing the analytical science aspect of NDD. The creation of these innovative tools and methodologies is on the verge of enabling new possibilities for clinical diagnostics, but is also faced with several hurdles that will be critically evaluated.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 24","pages":"5435 - 5448"},"PeriodicalIF":3.8,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benzoyl chloride derivatization improves selectivity and sensitivity of lipidomic quantitation in human serum of pancreatic cancer patients using RP-UHPLC/MS/MS. 苯甲酰氯衍生化提高了RP-UHPLC/MS/MS法测定胰腺癌患者血清脂质组学定量的选择性和灵敏度。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-10-06 DOI: 10.1007/s00216-025-06151-0

Ondřej Peterka, Zuzana Lásko, Robert Jirásko, Petra Peroutková, Anna Taylor, Beatrice Mohelníková-Duchoňová, Irena Kozubíková, Martin Loveček, Bohuslav Melichar, Michal Holčapek

{"title":"Benzoyl chloride derivatization improves selectivity and sensitivity of lipidomic quantitation in human serum of pancreatic cancer patients using RP-UHPLC/MS/MS.","authors":"Ondřej Peterka, Zuzana Lásko, Robert Jirásko, Petra Peroutková, Anna Taylor, Beatrice Mohelníková-Duchoňová, Irena Kozubíková, Martin Loveček, Bohuslav Melichar, Michal Holčapek","doi":"10.1007/s00216-025-06151-0","DOIUrl":"https://doi.org/10.1007/s00216-025-06151-0","url":null,"abstract":"<p><p>Chemical derivatization is a powerful strategy for enhancing the chromatographic behavior and mass spectrometric sensitivity of lipids, which play an essential role in cellular processes and show high potential in cancer biomarker research. In this study, we describe a targeted and validated method that combines benzoyl chloride derivatization with reversed-phase ultrahigh-performance liquid chromatography tandem mass spectrometry (RP-UHPLC/MS/MS) for the quantitative analysis of the human serum lipidome. In total, 450 lipid species from 19 lipid subclasses were identified based on a combination of multiple reaction monitoring transitions, retention dependencies, dilution series, and derivatization tags. The developed methodology increases the sensitivity for most investigated lipid classes in comparison to conventional methods, but the highest improvement was observed for monoacylglycerols, diacylglycerols, sphingoid bases, and free sterols. The method's accuracy was confirmed using NIST SRM 1950, as the determined concentrations were in agreement with the consensus values from ring trials. Lipidomic profiling of clinical samples revealed a significant dysregulation of lipid metabolism in pancreatic cancer patients compared to healthy controls. Key findings included the upregulation of most monoacylglycerols and sphingosine, and a pronounced downregulation of sphingolipids with very long saturated N-acyl chains and phospholipids containing fatty acyl compositions 18:2 and 20:4. This targeted approach is consistent with the trends previously seen with other methods and also provides new findings and more detailed structural insights into metabolic alterations in pancreatic cancer.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly sensitive detection of miRNA based on a quencher-free fluorescence strategy with dual-signal amplification. 基于双信号放大的无猝灭荧光策略的高灵敏度miRNA检测。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-10-06 DOI: 10.1007/s00216-025-06119-0

Dan Wang, Shu-Ling Yan, Xiao-Tong Yang, Chun-Guang Yang, Zhang-Run Xu

{"title":"Highly sensitive detection of miRNA based on a quencher-free fluorescence strategy with dual-signal amplification.","authors":"Dan Wang, Shu-Ling Yan, Xiao-Tong Yang, Chun-Guang Yang, Zhang-Run Xu","doi":"10.1007/s00216-025-06119-0","DOIUrl":"https://doi.org/10.1007/s00216-025-06119-0","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) have emerged as crucial post-transcriptional regulators of gene expression and promising biomarkers for disease diagnosis and prognosis. Their aberrant expression levels are closely associated with the pathogenesis and progression of various human diseases. In this study, we developed a novel quencher-free dual-signal amplification strategy for highly sensitive miRNA detection. The approach involves the use of a target-specific padlock probe that undergoes miRNA-triggered circularization to initiate rolling circle amplification (RCA). The resulting RCA products then hybridize with 2-aminopurine-labeled hairpin probes, maintaining fluorescence quenching in the hybridized state. Upon introduction of exonuclease III, the enzyme-mediated cyclic cleavage enables duplex structures to release numerous 2-aminopurine molecules into the solution, generating a strong fluorescence signal through dual amplification (RCA and exonuclease III digestion). This innovative design eliminates the requirement for conventional quencher molecules while achieving high sensitivity, and the method has a detection limit of 63 aM for miRNA-21 and possesses single-base mismatch discrimination capability. The clinical applicability of the method was demonstrated through direct quantification of miRNA-21 in both human serum samples and tumor cell lysates without the need for total RNA extraction. These results validate the potential of the strategy for early cancer diagnosis.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adduct formation by coeluting endogenous biomolecules using ESI-MS : A comprehensive study on ESI efficiency. 利用ESI- ms对内源生物分子外溶形成加合物的研究

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-10-04 DOI: 10.1007/s00216-025-06130-5

Louis J Triebel, Daniela Wissenbach, Frank T Peters, Dirk K Wissenbach

{"title":"Adduct formation by coeluting endogenous biomolecules using ESI-MS : A comprehensive study on ESI efficiency.","authors":"Louis J Triebel, Daniela Wissenbach, Frank T Peters, Dirk K Wissenbach","doi":"10.1007/s00216-025-06130-5","DOIUrl":"https://doi.org/10.1007/s00216-025-06130-5","url":null,"abstract":"<p><p>Mass spectrometry plays a crucial role for qualitative and quantitative analysis in various scientific fields. For LC-MS, ESI is the most frequently used ionization technique. However, ionization processes are still not fully understood, and ion enhancement or ion suppression effects can usually be found during ESI. In the current work, adduct formation by coeluting (endogenous) compounds was observed for the diuretic hydrochlorothiazide during negative mode ionization, decreasing the corresponding deprotonated hydrochlorothiazide signal. After identification of the corresponding adduct partners, being hippuric acid and indoxyl sulfate, systematic investigations on this formation were conducted using different concentrations of both compounds. Additionally, APCI and different ESI sources, as well as different mobile phase compositions, were tested. It was shown that the adduct formation was only found by ESI and was independent of vendor-specific source design. The adduct formation was also independent of the composition of the mobile phases and was found to be dependent on the concentration of the coeluting compounds. An over-additive effect on hydrochlorothiazide signal loss was found if hippuric acid and indoxyl sulfate adducts were formed. Finally, adduct formation was studied for other structural related and non-structural related compounds. For compounds with a high(er) structural similarity to hydrochlorothiazide (namely: bendroflumethiazide, buthiazide, cyclopenthiazide, and dorzolamide), adduct formation was observed. This study provided new insights into the ESI process.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards sustainable lipidomics: computational screening and experimental validation of chloroform-free alternatives for lipid extraction. 迈向可持续脂质组学：计算筛选和实验验证的无氯仿替代脂质提取。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-10-04 DOI: 10.1007/s00216-025-06136-z

Andrea Venturi, Michele Wölk, Sider Penkov, Gabriele Cruciani, Maria Fedorova, Laura Goracci

{"title":"Towards sustainable lipidomics: computational screening and experimental validation of chloroform-free alternatives for lipid extraction.","authors":"Andrea Venturi, Michele Wölk, Sider Penkov, Gabriele Cruciani, Maria Fedorova, Laura Goracci","doi":"10.1007/s00216-025-06136-z","DOIUrl":"https://doi.org/10.1007/s00216-025-06136-z","url":null,"abstract":"<p><p>Sample preparation, particularly lipid extraction, plays a critical role in lipidomics workflows and strongly influences analytical outcomes. In biomedical research, the most commonly used lipid extraction protocols rely on chloroform due to its favorable physicochemical properties, including the ability to dissolve both polar and apolar lipids, as well as its high volatility. Although well-established chloroform-based methods enable high recovery of a broad range of lipids, concerns about the toxicity and environmental impact of chloroform necessitate the development of more sustainable alternatives. In this study, a combined computational and experimental strategy was employed to identify and validate greener solvents suitable for lipid extraction in lipidomics applications. Solvent selection was guided by Hansen solubility parameters, Abraham solvation descriptors, and principal component analysis, yielding five candidate solvents as potential chloroform alternatives. Evaluation of solvent sustainability and human health risk was conducted using CHEM21 criteria and supplemented by a comprehensive literature review. Initial validation of extraction efficiency for identified candidates was performed using synthetic lipid standards in the absence of a biological matrix, followed by testing lipid extraction from human plasma using both monophasic and biphasic extraction protocols. The highest extraction efficiency was achieved using a single-phase extraction method based on cyclopentyl methyl ether, which exhibited comparable and even superior performance to the conventional chloroform-based Folch protocol. Overall, this study highlights the feasibility of replacing chloroform with less hazardous solvents in lipidomics workflows without compromising analytical performance, and provides a comparative assessment of computational solvent prediction approaches for green chemistry applications.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A two-signal aptasensor for sensitive exosome detection via rolling circle amplification and G-quadruplex structure. 一种基于滚动圆放大和g -四重结构的灵敏外泌体检测双信号适体传感器。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-10-03 DOI: 10.1007/s00216-025-06115-4

Xu Sun, Qing Dong, Xuemei Zhang, Fan Feng, Junliang Chang, Dan Li, Jin Wang, Erkang Wang

{"title":"A two-signal aptasensor for sensitive exosome detection via rolling circle amplification and G-quadruplex structure.","authors":"Xu Sun, Qing Dong, Xuemei Zhang, Fan Feng, Junliang Chang, Dan Li, Jin Wang, Erkang Wang","doi":"10.1007/s00216-025-06115-4","DOIUrl":"https://doi.org/10.1007/s00216-025-06115-4","url":null,"abstract":"<p><p>Exosomes play a pivotal role in cancer diagnosis and therapy, yet their clinical application faces significant technical challenges. To address this, we developed a sensitive, aptamer-based platform that integrates nucleic acid amplification and a G-quadruplex (G4) with two-signal output for exosome detection. Our approach employs CD63 antibody-conjugated magnetic beads (MBs) for efficient exosome capture and purification. The captured exosomes are then bound by CD63-specific aptamers embedded in rolling circle amplification (RCA) products, forming a sandwich complex with the MBs. The RCA products also contain G4 repeats, enabling two-signal output through interaction with Fe(III)-protoporphyrin IX (Hemin) and N-methylporphyrin-dipropionic acid IX (NMM). The G4-Hemin complex catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), producing a colorimetric signal, while the G4-NMM enhances fluorescence emission. This detection system achieves high sensitivity, with detection ranges of 4.0 × 10<sup>3</sup> to 4.0 × 10<sup>7</sup> particles/mL (colorimetric) and 4.0 × 10<sup>2</sup> to 4.0 × 10<sup>6</sup> particles/mL (fluorescence), and low detection limits of 1.78 × 10<sup>3</sup> particles/mL (colorimetric) and 96 particles/mL (fluorescence). Demonstrating high performance in complex media (10% exosome-depleted fetal bovine serum, FBS), this biosensor achieves sensitivity comparable to current methods at a low cost ($5.19 per test). This combination of robustness, sensitivity, and affordability makes it a highly promising platform for clinical exosome-based diagnostics.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sterol analysis in cancer cells using atmospheric pressure ionization techniques. 用常压电离技术分析癌细胞中的甾醇。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-10-02 DOI: 10.1007/s00216-025-06126-1

Pia Wittenhofer, Juan F Ayala-Cabrera, Laila Orell, Florian Uteschil, Sven W Meckelmann, Oliver J Schmitz

{"title":"Sterol analysis in cancer cells using atmospheric pressure ionization techniques.","authors":"Pia Wittenhofer, Juan F Ayala-Cabrera, Laila Orell, Florian Uteschil, Sven W Meckelmann, Oliver J Schmitz","doi":"10.1007/s00216-025-06126-1","DOIUrl":"https://doi.org/10.1007/s00216-025-06126-1","url":null,"abstract":"<p><p>The analysis of sterol biosynthesis poses analytical challenges such as vast concentration differences between precursor molecules and cholesterol as well as the substantial interferences between the sterols and the matrix, resulting in strong ion suppression. LC-MS and 2D-LC-MS may address some of these challenges, but for sensitive and robust detection, ionization plays a key role. Electrospray ionization (ESI) stands out for its capability to generate ions from polar and nonpolar compounds, rendering it suitable for a broad spectrum of analytes, including sterols. Atmospheric pressure chemical ionization (APCI) demonstrates efficacy for compounds exhibiting moderate to low polarity, thereby facilitating efficient ionization of sterols. Tube plasma ionization (TPI) is a relatively new technique that promises sensitive ionization across a wide polarity range, indicating its potential utility for sterol analysis. Here, a new tube plasma ion source for LC-MS was developed and compared with established ion sources (ESI and APCI) for sterol analysis. The TPI source showed comparable LOQs to APCI and clearly outperformed ESI in terms of sensitivity. Both TPI and APCI provided stable signals during extended measurement series, while ESI suffered from pronounced ion suppression. Application to human plasma, HepG2 cells, and liver tissue demonstrated that TPI provided results in close agreement with APCI, highlighting its robustness for sterol quantification in complex biological matrices.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0