Analytical and Bioanalytical Chemistry最新文献_第3页

Single compound data supplementation to enhance transferability of fermentation specific Raman spectroscopy models. 补充单一化合物数据以提高发酵特定拉曼光谱模型的可转移性。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-04-01 Epub Date: 2025-02-06 DOI: 10.1007/s00216-025-05768-5

Maarten Klaverdijk, Marcel Ottens, Marieke E Klijn

{"title":"Single compound data supplementation to enhance transferability of fermentation specific Raman spectroscopy models.","authors":"Maarten Klaverdijk, Marcel Ottens, Marieke E Klijn","doi":"10.1007/s00216-025-05768-5","DOIUrl":"10.1007/s00216-025-05768-5","url":null,"abstract":"Raman spectroscopy is a valuable analytical tool for real-time analyte quantification in fermentation processes. Quantification is performed with chemometric models that translate Raman spectra into concentration values, which are typically calibrated with process data from multiple comparable fermentations. However, process-specific models underperform for minor process variation(s) or different operation modes due to the integration of cross-correlations, resulting in low target analyte specificity. Thus, model transferability is poor and labor-intensive (re-)calibration of models is required for related processes. In this work, partial least-squares models for glucose, ethanol, and biomass were calibrated with Saccharomyces cerevisiae batch fermentation data and subsequently transferred to a fed-batch operation. To enhance model transferability without additional process runs, single compound data supplementation was performed. The supplemented models increased overall target analyte specificity and demonstrated sufficient prediction accuracy for the fed-batch process (root-mean-square errors of prediction (RMSEP) of 3.06 mM, 8.65 mM, and 0.99 g/L for glucose, ethanol, and biomass), while maintaining high prediction accuracy for the batch process (RMSEP of 1.71 mM, 4.20 mM, and 0.17 g/L for glucose, ethanol, and biomass). This work showcases that process data in combination with single compound spectra is a fast and efficient strategy to apply Raman spectroscopy for real-time process monitoring across related processes.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1873-1884"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cost-effective, user-friendly detection and preconcentration of thrombin on a sustainable paper-based electrochemical platform. 具有成本效益，用户友好的检测和凝血酶在可持续纸基电化学平台上的预浓缩。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-04-01 Epub Date: 2025-02-01 DOI: 10.1007/s00216-025-05764-9

Ada Raucci, Giuseppina Sorrentino, Sima Singh, Nicola Borbone, Giorgia Oliviero, Gennaro Piccialli, Monica Terracciano, Stefano Cinti

{"title":"Cost-effective, user-friendly detection and preconcentration of thrombin on a sustainable paper-based electrochemical platform.","authors":"Ada Raucci, Giuseppina Sorrentino, Sima Singh, Nicola Borbone, Giorgia Oliviero, Gennaro Piccialli, Monica Terracciano, Stefano Cinti","doi":"10.1007/s00216-025-05764-9","DOIUrl":"10.1007/s00216-025-05764-9","url":null,"abstract":"Thrombin overexpression in serum serves as a critical biomarker and is implicated in several diseases associated with significant morbidity and mortality. Existing techniques for thrombin detection are time-consuming and require sophisticated equipment and extensive sample preparation procedures, which further delay the detection and increase the cost of the procedure. Early and accessible diagnosis at the point of care, especially in limited-resource countries, represents the first step of clinical interventions. To overcome these limitations, we have proposed an innovative, sustainable paper-based electrochemical detection platform for thrombin. In this work, a sustainable paper-based aptasensor was rationally designed, characterized, evaluated against conventional gold standard plastic-based substrates, and applied to human serum, yielding a detection limit of ~ 60 pM. The present method provides an efficient and user-friendly way for the detection of thrombin and potentially leading to better management and treatment outcomes for patients.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1863-1872"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitation of antibiotics in fresh fermentation medium by hydrophilic interaction chromatography mass spectrometry. 用亲水性相互作用色谱-质谱法定量新鲜发酵培养基中的抗生素。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-04-01 Epub Date: 2025-02-21 DOI: 10.1007/s00216-025-05775-6

Nadia Marcon, Mathias Rüdt, Joachim Klein, Saša M Miladinović

引用次数: 0

Oxygen enhanced plasma discharge and its application as carrier gas for high-field asymmetric ion mobility spectroscopy. 氧增强等离子体放电及其作为载气在高场不对称离子迁移谱中的应用。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-04-01 Epub Date: 2025-03-11 DOI: 10.1007/s00216-025-05799-y

Hua Li, Yuqiao Zhang, Xiaoxia Du, Wenxiang Xiao

引用次数: 0

Temporal resolved multi-proteomic analysis enabled the systematic characterization of N-glycosylation pattern changes during Jurkat T cell activation. 时间分辨多蛋白质组学分析能够系统地表征Jurkat T细胞活化过程中n -糖基化模式的变化。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-04-01 Epub Date: 2025-02-25 DOI: 10.1007/s00216-025-05805-3

Mingming Dong, Xiaoyan Liu, Changrui Zhao, Zheng Fang, Zhongyu Wang, Xin Guo, Yan Wang, Yanan Li, Mingliang Ye, Lingyun Jia

{"title":"Temporal resolved multi-proteomic analysis enabled the systematic characterization of N-glycosylation pattern changes during Jurkat T cell activation.","authors":"Mingming Dong, Xiaoyan Liu, Changrui Zhao, Zheng Fang, Zhongyu Wang, Xin Guo, Yan Wang, Yanan Li, Mingliang Ye, Lingyun Jia","doi":"10.1007/s00216-025-05805-3","DOIUrl":"10.1007/s00216-025-05805-3","url":null,"abstract":"Protein glycosylation plays essential roles in regulating innate and adaptive immune response. Previous studies only focused on individual protein-glycan interactions or specific glycoform changes during T cell activation, yet the systematic characterization of protein glycosylation alterations remains insufficiently elucidated. To address these limitations, we conducted temporally resolved quantitative analysis of glycoforms, site-specific glycans, glycoproteins, and glycosylation enzymes in activated Jurkat T cells, and successfully portrayed the dynamic landscape of protein glycosylation during Jurkat T cell activation. We found the heterogeneity and number of significantly upregulated glycopeptides increased along with activation. For most glycopeptides, their alteration patterns did not correlate with the abundance of their glycoprotein substrates. However, functional molecules including CD69, CD28, and PTPRC demonstrated co-upregulation at both the protein and glycosylation levels. Correlation analysis between glycopeptides and glycotransferases indicated that sialylated or fucosylated peptides were well correlated with enzymes involved in glycan branching and capping. Comparative analysis of global peptides, glycopeptides, and phosphopeptides revealed their distinctive changing patterns along Jurkat T cell activation, and only glycosylation demonstrated a steady increase trend with a large proportion of upregulated glycopeptides. Collectively, this integrated multi-proteomics characterization of activated Jurkat T cells provided insights for the development of novel therapeutic strategy targeting glycosylation.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"2169-2183"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyrolysis-GC/MS differentiates polyesters and detects additives for improved monitoring of textile labeling accuracy and plastic pollution. 热解- gc /MS区分聚酯和检测添加剂，以提高纺织品标签准确性和塑料污染的监测。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-04-01 DOI: 10.1007/s00216-025-05851-x

Josh Forakis, Jennifer Lynch

{"title":"Pyrolysis-GC/MS differentiates polyesters and detects additives for improved monitoring of textile labeling accuracy and plastic pollution.","authors":"Josh Forakis, Jennifer Lynch","doi":"10.1007/s00216-025-05851-x","DOIUrl":"https://doi.org/10.1007/s00216-025-05851-x","url":null,"abstract":"Polyesters comprise the greatest proportion of textile fibers and are found in various everyday goods; hence, polyester fibers are a significant source of microplastic pollution and textile waste. The specific chemical composition of commercial polyester fibers is often proprietary and mostly assumed to be poly(ethylene terephthalate) (PET). Polyester is a class of polymers that include poly(butylene terephthalate) (PBT), poly(cyclohexylenedimethylene terephthalate) (PCT), and poly(ethylene naphthalate) (PEN), as well as biodegradable polymers. Our study aims to clarify whether household polyester products are primarily PET, are labeled accurately, or contain phthalate additives by applying double-shot pyrolysis-gas chromatography/mass spectroscopy (Py-GC/MS). We analyzed four scientific-grade polyester reference standards, 52 manufacturer-grade polyester fibers or pellets, and 229 samples from 193 consumer polyester products. From the pyrograms, samples were predominantly identified as PET (87.4%, 95% CI [93.5-81.3%]), but five samples were identified as a different polyester, nine as non-polyester polymers, and 23 as a blend of PET with another polymer. From the thermal desorption chromatograms, diethyl phthalate was the most frequently detected phthalate, found in 23.3% (95% CI [17.3-29.3%]) of the consumer products, including children's toys. Double-shot py-GC/MS advantageously results in these empirical data that (1) counter the assumption that products labeled polyester are always PET, (2) emphasize the importance of creating spectral libraries with well-characterized materials for accurate polymer identification of unknown plastic particles, and (3) demonstrate that phthalates are common additives in household products.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing the discovery bioanalysis strategy for macrocyclic peptides. 优化大环肽的发现生物分析策略。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-04-01 Epub Date: 2025-02-15 DOI: 10.1007/s00216-025-05781-8

Xing Zhang, Stephanie Dale, Yusi Cui, Joe Napoli, Huy Nguyen, Jingwei Cai, Brian Dean

{"title":"Optimizing the discovery bioanalysis strategy for macrocyclic peptides.","authors":"Xing Zhang, Stephanie Dale, Yusi Cui, Joe Napoli, Huy Nguyen, Jingwei Cai, Brian Dean","doi":"10.1007/s00216-025-05781-8","DOIUrl":"10.1007/s00216-025-05781-8","url":null,"abstract":"Macrocyclic peptides (MCPs) have remained a compelling modality in drug discovery and development, with many successful marketed drugs. Their unique molecular structure and ADME properties have posed bioanalytical challenges that cannot be fully addressed with conventional small molecule LC-MRM assays. In this work, we developed and optimized a high-throughput discovery bioanalytical strategy for MCPs with 16 marketed MCP drugs. By evaluating ten different sample extraction methods based on the recovery and matrix effect, we identified that the protein precipitation extraction with MeOH/ACN (1/1 v/v) with 0.5% FA outperformed the other sample extraction methods, achieving 80% recovery for 80% of the MCP drugs and 90% matrix effect for 90% of the MCP drugs. By assessing the sensitivity of the targeted-selected ion monitoring (t-SIM) and parallel reaction monitoring (PRM) on the Orbitrap HRMS and comparing with the conventional LC-MRM, we concluded that the t-SIM provided comparable sensitivity with MRM (LOQ at 1~3 ng/mL for the majority of the MCP drugs), with the extra benefits of minimal method development and high post-acquisition flexibility in data processing. The optimized bioanalytical strategy was applied to various biological matrices and displayed performance that met the quantitation requirements for discovery bioanalysis.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"2015-2027"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive MALDI mass spectrometry imaging of tumor regions post-neoadjuvant therapy. 新辅助治疗后肿瘤区域的综合MALDI质谱成像。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-04-01 Epub Date: 2025-02-20 DOI: 10.1007/s00216-025-05785-4

Hui Xu, Qian Zhang, Jie Yuan, Jianfeng Xu, Jian Sui, Jia Liu

引用次数: 0

Self-protective DNAzyme-based dual-responsive three-way Y-probe for simultaneous determination of multiple pathogenic bacteria. 基于dnazyme的自保护双响应三向y探针同时检测多种致病菌。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-04-01 Epub Date: 2025-02-17 DOI: 10.1007/s00216-025-05782-7

Bowen Li, Xinru Ren, Yijing Xiao, Weiqing Sun, Meili Yang, Tsing-Ao Pang, Rui Zhu, Zhiqiang Guo, Yu Wang, Su Liu, Jiadong Huang

{"title":"Self-protective DNAzyme-based dual-responsive three-way Y-probe for simultaneous determination of multiple pathogenic bacteria.","authors":"Bowen Li, Xinru Ren, Yijing Xiao, Weiqing Sun, Meili Yang, Tsing-Ao Pang, Rui Zhu, Zhiqiang Guo, Yu Wang, Su Liu, Jiadong Huang","doi":"10.1007/s00216-025-05782-7","DOIUrl":"10.1007/s00216-025-05782-7","url":null,"abstract":"Foodborne pathogens, a major cause of foodborne illness due to their high virulence, pose a serious threat to public health. Consequently, identification of foodborne pathogens is essential for the prevention and treatment of foodborne infections. Consequently, there is an immediate need to establish a highly specific and precise approach for the concurrent detection of several foodborne pathogens. Herein, we developed a DNAzyme-based self-protecting dual-response nanoprobe for the simultaneous detection of two foodborne pathogens. The technique utilizes nanostructures to achieve logical signal input and output. In the presence of the target pathogen, the pathogen binds to the arch probe and releases the activation chain, which in turn activates a strand-displacement reaction and DNAzyme for signal amplification, producing different output signals to complete the simultaneous detection of multiple pathogens. The limits of detection for E. coli O157:H7 and S. typhimurium were determined to be 3.7 cfu/mL and 3.2 cfu/mL, with a measurement response time of 2 h. This approach enables ultrasensitive, specific, and simultaneous detection of two foodborne pathogens and is applicable for identifying foodborne pathogens in actual biological samples. The fluorescence detection of foodborne pathogens with a three-way Y-probe and DNAzyme coupling represents a novel approach for the concurrent identification of several foodborne diseases.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1779-1790"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-analytic assessment of dried blood and dried plasma spots: integration in mass spectrometry-based metabolomics and lipidomics workflow. 干血和干血浆斑点的分析前评估：基于质谱的代谢组学和脂质组学工作流程的整合。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-04-01 Epub Date: 2025-02-05 DOI: 10.1007/s00216-025-05760-z

Eleonora Bossi, Simone Serrao, Pierluigi Reveglia, Antonietta Ferrara, Marta Nobile, Elena Limo, Gaetano Corso, Giuseppe Paglia

{"title":"Pre-analytic assessment of dried blood and dried plasma spots: integration in mass spectrometry-based metabolomics and lipidomics workflow.","authors":"Eleonora Bossi, Simone Serrao, Pierluigi Reveglia, Antonietta Ferrara, Marta Nobile, Elena Limo, Gaetano Corso, Giuseppe Paglia","doi":"10.1007/s00216-025-05760-z","DOIUrl":"10.1007/s00216-025-05760-z","url":null,"abstract":"Microsampling, especially dried blood spots (DBS), emerged in recent years as a viable alternative to conventional blood collection since it is rapid, simple, minimally invasive, and has user-friendly characteristics. Moreover, DBS are able to avoid analyte degradation thanks to their great stability. Due to their versatility, clinical applications with DBS have increased, including mass spectrometry-based metabolomics and lipidomics studies. In this work, we evaluated and optimized extraction protocols testing five different extraction solutions to perform metabolomics and lipidomics studies on the same spot considering three commercially available microsampling devices, Capitainer, Whatman, and Telimmune. Parallelly, we also evaluated the short-term stability of the three devices at room temperature for up to 5 days. Our results showed that pure methanol was the best compromise to simultaneously extract from the same spot both the lipidome and polar metabolome. However, we also propose a two-step protocol combining methanol and water extraction that improves polar metabolite extraction and shows improved reproducibility in Capitainer and Whatman. Short-term stability results highlighted that both polar metabolites and lipids were stable for up to 6 days using the Capitainer device, while with Whatman and Telimmune, some significant variations were observed after 3 days for some classes of metabolites/lipids, suggesting the need for cold-chain storage when working with these devices.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"1791-1805"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0