Analytical and Bioanalytical Chemistry最新文献

{"title":"Polymeric deep eutectic solvents-functionalized carbon nanotubes: a synergistic buffer additive for enhanced enantioseparation in capillary electrophoresis.","authors":"Chengchen Zhang, Yanfeng Guo, Shunli Ji, Ying Lu, Xiaofei Ma, Yan Gu","doi":"10.1007/s00216-026-06852-0","DOIUrl":"https://doi.org/10.1007/s00216-026-06852-0","url":null,"abstract":"<p><p>The application of carbon nanotubes (CNTs) in capillary electrophoresis (CE) for chiral separation is often limited by their poor dispersibility, tendency to aggregate, and lack of enantioselectivity. This study reports the preparation of a class of polymeric deep eutectic solvents (PDES) modified hydroxylated carbon nanotube composites (PDES@CNT) via an aqueous solution copolymerization method and their application as pseudostationary phase for chiral separation in CE. The proposed materials were characterized by nuclear magnetic resonance, infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, transmission electron microscopy, and the Tyndall effect. The results demonstrate that the modification of PDES significantly enhances the dispersibility and electrophoretic stability of CNTs in aqueous buffers, effectively improving the issues of CNTs aggregation and separation repeatability. Compared with the single methyl-β-cyclodextrin (M-β-CD) system, the common DESs/M-β-CD systems, and the common DESs@CNTs systems, the PDES@CNTs systems exhibit superior separation performance for some studied model drugs. The potential mechanism of PDES@CNTs for improving separation was discussed through molecular simulation. The PDES@CNTs systems may construct an efficient chiral microenvironment through multiple intermolecular interactions, thereby enhancing enantioselective recognition capability.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implementation of a mixed-mode liquid chromatography-based analysis to assess fluorinated inorganic anions and (ultra)-short chain PFAS contamination in surface waters.","authors":"Miriam Haußecker, Simon Bertels, Bernat Oró-Nolla, Christian Zwiener","doi":"10.1007/s00216-026-06841-3","DOIUrl":"https://doi.org/10.1007/s00216-026-06841-3","url":null,"abstract":"<p><p>Ultra-short-chain (USC) per- and polyfluoroalkyl substances (PFAS) are polar, mobile, and persistent and are increasingly focused on because of their widespread occurrence in various environmental matrices. One reason why they were previously often overlooked is their insufficient retention in reversed-phase liquid chromatography which is the standard analytical method in most laboratories for PFAS analysis. In this study, a method using mixed-mode liquid chromatography (MMLC) coupled to tandem mass spectrometry (MS/MS) was optimized for the detection and quantification of five USC PFAS (C₂ and C₃ perfluorocarboxylic acids (PFCAs) and C₁ to C₃ perfluorosulfonic acids (PFSAs)), four short-chain (SC) PFAS (C₄-C₆ PFCAs and C₄ PFSA), bis(trifluoromethane)sulfonimide (NTf2), and the three fluorinated inorganic anions (FIAs): tetrafluoroborate (BF₄^-), hexafluorophosphate (PF₆^-), and hexafluoroarsenate (AsF₆^-). To investigate the conditions for optimal retention, different eluent constitutions were tested combining varying solvents and additives. USC PFAS could be analyzed using neutral conditions in the aqueous (A) and basic conditions in the organic eluent (B) whereas FIAs were measured with acidified A and basic B. The method was validated and matrix effects were corrected with mass-labeled standards and standard addition. The method was successfully applied to three surface water samples in which eight PFAS could be quantified. TFA and PF₆^- were found with the highest concentrations at 2.70 µg/L and 0.287 µg/L, respectively, while all other target PFAS occurred in the lower ng/L range. Thus, the sum concentration of TFA and PF₆^- accounted for more than 97% of the total PFAS concentration in all samples.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time process analysis and optimization of nitroguanidine synthesis in microflow using inline Raman spectroscopy.","authors":"L Hirschberger, M B R Völkl, K Karaghiosoff, T M Klapötke, C Haisch","doi":"10.1007/s00216-026-06847-x","DOIUrl":"https://doi.org/10.1007/s00216-026-06847-x","url":null,"abstract":"<p><p>Non-contact inline Raman spectroscopy is used as a fast, flexible, and safe method for monitoring and optimizing continuous-flow processes involving highly hazardous and corrosive materials. The synthesis of nitroguanidine (NQ), an energetic material, was characterized and optimized as a model for hazardous reactions. The contactless, flexible, and real-time analysis in this environment was implemented using a clip-on adapter for a Raman probe downstream of the microflow reactor. Initial experiments focus on characterizing the in situ formation of the nitration agent, the nitronium ion (NO₂⁺), and identifying an optimal nitric acid volume fraction between 10 and 20% v/v for maximum NO₂⁺ yield. The PAT setup was then used to rapidly optimize reaction parameters (residence time, reactor temperature, and stoichiometry) to enhance reactor output while maintaining high yield. Increasing the reaction temperature to 70 °C effectively reduces system pressure due to the lower viscosity of the reaction stream, while simultaneously increasing yield. Reduced residence times enable higher output with only minimal yield loss (6.3 min, 97%). The analysis of the molar ratio of the reagents further revealed that using a slight excess of nitric acid maintains the yield while avoiding product losses due to back reaction during subsequent processing. To verify the long-term repeatability of the reactor for synthesis, the reaction was performed several times under optimized conditions.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep eutectic solvent-assisted electropolymerization of MXene-based dual-monomer surface molecularly imprinted sensor for selective detection of myricetin.","authors":"Yuxin Xie, Zhiwei Ding, Shakeel Zeb, Liangyin Xiang, Rongpeng Yu, Zhanchao Liu, Yinhua Jiang, Yan Liu","doi":"10.1007/s00216-026-06831-5","DOIUrl":"https://doi.org/10.1007/s00216-026-06831-5","url":null,"abstract":"<p><p>A novel surface molecularly imprinted electrochemical sensor for myricetin detection was developed. This sensor employs deep eutectic solvent (DES) as a green multifunctional electrolyte to suppress template oxidation, improve solubility, and assist the electropolymerization of a dual-monomer (p-aminobenzoic acid, p-ABA; pyrrole, Py). The sensor with DES as the electrolyte exhibited a 1.3-fold higher response current than the aqueous system, owing to enhanced myricetin solubility and reduced template oxidation. Meanwhile, it was incorporated with a well-dispersed few-layer MXene to construct an optimal sensitized interface. The dual-monomer system is capable of two types of interactions with myricetin: p-ABA forms hydrogen bonds with its hydroxyl group, while Py undergoes π-π stacking with the aromatic a-ring. This synergistic binding generates an imprinted cavity with high specificity. The amount of sorbent, pH, and voltammetric parameters were all optimized during the experiment. Differential pulse voltammetry (DPV) showed that the sensor exhibited acceptable stability, a wide linear detection range of 2.0-160 µmol/L, and a low limit of detection (LOD) of 0.5 µmol/L (signal-to-noise ratio [S/N] = 3), with an imprinting factor of 3.4. The electrochemical investigation of myricetin in real fruit juice samples showed potential for the proposed sensor, which could have applications in food safety.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate determination of vitamin B₁₂ in infant formula using isotope dilution-ultrahigh-performance liquid chromatography-tandem mass spectrometry.","authors":"Hyeonwoo Yu, Yared Getachew Lijalem, Jaehyoung Koo, Sangdoo Ahn, Byungjoo Kim, Kihwan Choi","doi":"10.1007/s00216-026-06829-z","DOIUrl":"https://doi.org/10.1007/s00216-026-06829-z","url":null,"abstract":"<p><p>A method for the accurate determination of vitamin B₁₂ (cobalamin) in infant formula was developed using isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS). As an internal standard, a [¹³C₇]-cyanocobalamin isotope-labeled analogue was used. Because of the hygroscopic property of cobalamins, the concentration of the cyanocobalamin standard solution was determined via quantitative nuclear magnetic resonance (qNMR) spectroscopy and further validated by capillary electrophoresis with ultraviolet detection as an independent technique. The cobalamins contained in infant formula were converted to cyanocobalamin in the presence of cyanide. Incomplete conversion of cobalamins was observed, and the residual amount of hydroxocobalamin was quantified to obtain the total amount of cobalamins. The expanded measurement uncertainty ranged from 1.7% to 2.4%, accounting for the contribution of residual hydroxocobalamin. Recovery, calculated by comparing the measured CN-Cbl concentration with the gravimetric fortification level, ranged from 101 to 103%. Both intra- and inter-day RSDs were below 1%. The method was applied to commercially available infant formula, with cobalamin concentrations ranging from 31.0 to 40.6 µg/kg.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sub-3 μm porous graphitic carbon for highly polar metabolomics: orthogonal selectivity and comparison with HILIC and reversed-phase chromatography.","authors":"Danila La Gioia, Emanuela Salviati, Fabrizio Merciai, Vicky Caponigro, Vincenzo Vestuto, Carlo Crescenzi, Pietro Campiglia, Eduardo Maria Sommella","doi":"10.1007/s00216-026-06846-y","DOIUrl":"https://doi.org/10.1007/s00216-026-06846-y","url":null,"abstract":"<p><p>The analysis of the polar metabolome remains a major challenge in LC-MS. Beyond established hydrophilic interaction liquid chromatography (HILIC) and reversed-phase (RP) workflows, alternative and orthogonal chromatographic approaches are required to improve the detection and quantification of compounds with low logP values and structurally related isomers. In this study, we evaluated the performance of sub-3 µm porous graphitic carbon (PGC) stationary phase and benchmarked it against state-of-the-art HILIC and RP approaches. Flow rate, column temperature, organic modifier, mobile-phase composition, and pH were optimized using a representative panel of highly polar endogenous metabolites. The three chromatographic modes exhibited markedly complementary selectivity profiles. HILIC provided the broadest coverage of nucleotides and related metabolites, whereas PGC showed enhanced performance for sugar phosphates, polyamines, and, particularly, tricarboxylic acid (TCA) cycle intermediates. Focused optimization for TCA cycle metabolites improved peak shape and signal-to-noise ratio and enabled the separation of citrate and isocitrate, outperforming both HILIC and reversed-phase chromatography for the detection of this metabolite class under the investigated conditions. In mouse liver extracts, PGC detected a complementary subset of metabolites not observed using HILIC or reversed-phase chromatography, confirming its distinctive selectivity in a complex biological matrix. As a proof of concept, the optimized PGC method was applied to lipopolysaccharide-stimulated macrophages using a hybrid workflow combining multiplexed parallel reaction monitoring for the absolute quantification of TCA cycle intermediates with data-dependent acquisition for global metabolic profiling. The workflow captured the characteristic metabolic reprogramming associated with macrophage inflammatory activation. Overall, sub-3 µm PGC represents a valuable orthogonal chromatographic platform for extending analytical coverage and improving the characterization of highly polar metabolites.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a corn flour certified reference material for the accurate determination of aflatoxins.","authors":"Yared Getachew Lijalem, Hyeonwoo Yu, Jae Hyun Lee, Jaehyoung Koo, Mulugeta Derebew Damtew, Kihwan Choi, Byungjoo Kim","doi":"10.1007/s00216-026-06833-3","DOIUrl":"https://doi.org/10.1007/s00216-026-06833-3","url":null,"abstract":"<p><p>A corn flour certified reference material (CRM), KRISS CRM 108-01-013, was developed to support accurate measurement of aflatoxins (AFs). Corn flour naturally contaminated with AFs was mixed with AF-free corn flour to obtain a material with representative contamination levels. The mixed material was freeze-dried, milled, sieved, and thoroughly homogenized, and then dispensed into units containing approximately 15 g and stored at - 70 °C. Characterization was carried out using a validated isotope-dilution ultra-high-performance liquid chromatography-tandem mass spectrometry reference measurement procedure. Low between-bottle variability was observed, with relative standard deviations of 1.6 %, 1.0 %, 0.8 %, and 0.9 % for aflatoxin B₁ (AFB₁), aflatoxin B₂ (AFB₂), aflatoxin G₁ (AFG₁), and aflatoxin G₂ (AFG₂), respectively. Short- and long-term stability studies confirmed the stability of the target AFs under the investigated conditions; AFB₁ and AFG₂ were stable at - 70 °C for up to 18 months, whereas AFB₂ and AFG₁ were confirmed for 6 months at the same temperature. The certified mass fractions were (2.334 ± 0.086) µg/kg for AFB₁ and (0.1332 ± 0.0040) µg/kg for AFG₂, whereas AFB₂ and AFG₁ were assigned indicative values of (0.351 ± 0.010) µg/kg and (0.684 ± 0.022) µg/kg, respectively, because sufficient evidence of metrological traceability to the SI units could not be established. This CRM provides a metrologically traceable tool for the determination of AFB₁ and AFG₂ and indicative values for AFB₂ and AFG₁, thereby supporting quality assurance and validation of AF quantification in corn.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multifunctional gelatin-based bifunctional film for visual ratiometric fluorescence detection of hydrogen peroxide and antibacterial applications.","authors":"Xinru Zhao, Yimei Bai, Xuetao Zhang, Yu Hu, Xianjin Xia, Xingshuang Zhang, Guanchen Xu, Jing Zhang, Xiaoping Jiang, Meng Gao","doi":"10.1007/s00216-026-06839-x","DOIUrl":"https://doi.org/10.1007/s00216-026-06839-x","url":null,"abstract":"<p><p>Hydrogen peroxide (H₂O₂) is a widely used bleaching agent in food processing, and excessive intake of residual H₂O₂ can cause severe health risks to humans. Herein, a bifunctional gelatin film that combined H₂O₂ ratio fluorescence sensing and antibacterial capability was developed. Two functional units, aggregation-induced emission (AIE)-featured silica nanoprobe (TSN) with specific fluorescence response to H₂O₂ and europium-based complex Eu(AC)₃OP with antibacterial activity, were co-doped into a gelatin matrix, with glycerol used as a plasticizer. The ratio fluorescence film was finally fabricated via intermolecular hydrogen bonding cross-linking, which exhibited sensitive, selective, and visual ratiometric detection of H₂O₂, with a detection limit as low as 23 μM. It also achieved outstanding antibacterial activity against Escherichia coli and Staphylococcus aureus, with an inhibition rate up to 99%. This flexible film enables real-time monitoring of excessive residual H₂O₂ in chicken wings via adjacent placement. Moreover, the film exerts antibacterial activity on food surfaces to extend shelf life and maintain food quality. Furthermore, it can be naturally degraded in soil. Our work reveals that the proposed bifunctional film holds great promise in the fields of chemical analysis and food safety.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Quality assessment of Brazilian olive oils by GC×GC-MS and chemometrics.","authors":"Andre Cunha Paiva, Glaucimar Alex Passos de Resende, Luidy Darllan Barbosa, Daniel Lucas Dantas Freitas, Guilherme Post Sabin, Leandro Wang Hantao","doi":"10.1007/s00216-026-06853-z","DOIUrl":"https://doi.org/10.1007/s00216-026-06853-z","url":null,"abstract":"","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Candida albicans by a sandwich assay using boronate affinity microspheres coupled with amphotericin B-time-resolved fluorescent microspheres.","authors":"Han-Yue Li, Han Zhang, Zheng-Xiao Cai, Ming-Ze Yin, Xian-Hua Wang, Rui-Xue Ran","doi":"10.1007/s00216-026-06849-9","DOIUrl":"https://doi.org/10.1007/s00216-026-06849-9","url":null,"abstract":"<p><p>Candida albicans (C. albicans) is an opportunistic pathogenic fungus that poses a threat to public health and food safety, while conventional detection methods suffer from high costs, complicated procedures, and insufficient sensitivity. Here, a novel biomolecule-free sandwich detection platform was constructed to quantitatively detect C. albicans by integrating phenylboronic acid-functionalized magnetic microspheres (PFSEPD) and amphotericin B-labeled time-resolved fluorescent microspheres (ATFM). The stepwise synthesis of PFSEPD was verified through multiple characterizations, and the as-prepared PFSEPD exhibited favorable monodispersity, rapid magnetic responsiveness, and low preparation cost, with adsorption data well fitted by the Langmuir isotherm and pseudo-second-order kinetic models. Meanwhile, the ATFM signal probe was synthesized by covalently grafting amphotericin B (AmB) onto carboxylated time-resolved fluorescent microspheres (TFM). In the established dual-chemical recognition sandwich system, PFSEPD rapidly captured the fungus through boronate-cis-diol specific affinity, while ATFM selectively labeled the captured C. albicans through AmB-ergosterol interactions. After elution with SDS buffer, the fluorescent signal of the released ATFM was quantitatively measured. The PFSEPD-ATFM detection strategy achieved limits of detection (LODs) of 46 and 40 CFU mL^-1 in milk and honey, respectively, with recoveries of 96.2%-101.8% and relative standard deviations (RSDs) less than 5.0%. This dual-chemical recognition sandwich system provides a facile, sensitive alternative for the rapid and quantitative detection of C. albicans.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}