Analytical and Bioanalytical Chemistry最新文献

{"title":"Solution to structural analogy challenge","authors":"Reinhard Meusinger","doi":"10.1007/s00216-025-06050-4","DOIUrl":"10.1007/s00216-025-06050-4","url":null,"abstract":"","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 24","pages":"5361 - 5362"},"PeriodicalIF":3.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145284464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crown ether-bridging tetra-cyanostilbene macrocycle: an effective \"turn-on\" fluorescent sensor for the pesticide carbendazim.","authors":"Hongyu Guo, Yumeng Hou, Xiaoting Fang, Liangbin Lin, Fafu Yang","doi":"10.1007/s00216-025-06145-y","DOIUrl":"https://doi.org/10.1007/s00216-025-06145-y","url":null,"abstract":"<p><p>Carbendazim is widely used as a fungicide in various crops, but its residues might pose a threat to human health and environmental safety. It is significantly expected to develop a simple, sensitive, stable, and on-site method for detecting carbendazim. In this work, the first organic fluorescent sensor for carbendazim was designed and synthesized based on a crown ether-bridging tetra-cyanostilbene macrocycle. This macrocycle exhibited dark-blue fluorescence in THF-H₂O (5:95) solution, which was transformed into bright-blue fluorescence after sensing carbendazim. High sensing selectivity for carbendazim was observed among 24 kinds of pesticides and ions. The detection limit was as low as 4.22 × 10^-8 M. The sensing mechanism was deduced as two carbendazim molecules being encapsulated in the macrocyclic cavity based on multiple hydrogen bonds, π-π stacking, and hydrophobic action, resulting in stronger rotation restriction of aromatic groups and then higher fluorescence emission. The sensing application on test paper, real samples, and simulated water samples indicated excellent qualitative and quantitative detecting capabilities for sensing carbendazim, implying a good application prospect for simple, sensitive, stable, and on-site detection of carbendazim in daily life.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of small (1-10 µm) microplastic particles in soil matrices using automated scanning electron microscopy: possibilities and limitations.","authors":"Ralf Kaegi, Matthias Philipp, Isabel S Jüngling, Natalia P Ivleva, Thomas D Bucheli","doi":"10.1007/s00216-025-06111-8","DOIUrl":"https://doi.org/10.1007/s00216-025-06111-8","url":null,"abstract":"<p><p>Scanning electron microscopy (SEM) provides superb resolution power and allows distinguishing microplastic particles (MPs) from other particles based on elemental ratios derived from energy dispersive x-ray (EDX) analysis. Polycarbonate (PC) membranes are typically used to isolate MPs from suspensions for SEM investigations. This, however, results in poor (atomic weight) contrast between MPs and the underlying PC membrane, challenging automated particle detection. To introduce an elemental contrast between MPs and the PC membrane, a gold (Au) coating was applied to the PC membrane before use. Monte Carlo simulations conducted to optimize operational conditions of the SEM suggest an acceleration voltage of 3 kV in combination with a 40 nm Au coating of PC membranes as most appropriate. Stock suspensions made from polyethylene (PE) fragments, polyvinyl chloride (PVC) fragments, and standard soils, all sieved to 0.45-10 µm, were mixed at different ratios. Selected mixtures were density separated to isolate the MPs from the mixtures. Whereas up to 7% of PE was detected in PVC stocks, likely representing false positives, only negligible amounts of PVC were detected in PE stocks. In soils, neither PE nor PVC was detected in significant amounts. Polyethylene and PVC particles diluted in soil suspensions at particle number ratios of 1:1:2 and 1:1:2000 were density separated, and largely concentration independent recoveries of around 70% and 50% were obtained for PE and PVC, respectively. Good agreement between SEM-EDX and Raman measurements was obtained for MPs, underlining the strength of our approach to address the smallest size fraction of MPs in complex matrices.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Legislative chemistry challenge","authors":"Juris Meija","doi":"10.1007/s00216-025-06069-7","DOIUrl":"10.1007/s00216-025-06069-7","url":null,"abstract":"","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 24","pages":"5359 - 5360"},"PeriodicalIF":3.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145284461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart microfluidic devices integrated in electrochemical point-of-care platforms for biomarker detection in biological fluids.","authors":"Vincenzo Mazzaracchio, Fabiana Arduini","doi":"10.1007/s00216-025-06127-0","DOIUrl":"https://doi.org/10.1007/s00216-025-06127-0","url":null,"abstract":"<p><p>The increasing demand for decentralized, accessible, and rapid analytical tools is driving a transformation in healthcare toward point-of-care (POC) analytical technologies. The final aim is to reduce the cost of healthcare management originating from frequent patient hospitalizations and expensive and time-consuming laboratory-based analyses. This review explores the integration of microfluidic technologies with electrochemical sensing platforms, aiming to address the urgent need for POC analytical platforms. Owing to the miniaturization of fluid management systems and exploiting fluid automation, microfluidic devices enable low sample consumption, cost-effective analysis, and multiplexed detection, offering promising tools for real-time health monitoring. Among the other materials, the most commonly used substrates for microfluidics fabrication are paper, PDMS, and adhesive tape, which support custom-designed microchannel architectures, passive fluid motion, and wearable integration. Special attention is given to wearable sensors for sweat analysis, with various approaches employing capillary-driven flows and smart microfluidic designs to enable continuous and autonomous monitoring of biomarkers. Highlighting relevant works from the last 5 years, the review explores the role of integrated microfluidic electrochemical sensing devices in delivering advanced decentralized analytical platforms, with significant potential for clinical use in biomarker detection.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and sensitive detection of polycyclic aromatic hydrocarbons using electrochemically assisted derivatization mass spectrometry.","authors":"Heng-Yu Xu, Zhi-Cai Jiang, Cheng-Fei Lu, Mao-Song Li, Zhao-Ding Chen, Li-Lan Liu, Shu-Ting Xu, Bo Liu, Jun Hu","doi":"10.1007/s00216-025-06160-z","DOIUrl":"https://doi.org/10.1007/s00216-025-06160-z","url":null,"abstract":"<p><p>Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous toxic pollutants that pose significant risks to human health. However, because of their intrinsic non-polar properties, only moderate detection sensitivity can be achieved in the mass spectrometric detection of PAHs. In this work, we presented a novel electrochemically assisted derivatization nano-electrospray ionization mass spectrometry (ECAD-nESI-MS) method to permit the rapid and ultrasensitive analysis of PAHs in complicated samples. In ECAD-nESI-MS, the in situ electrochemical derivatization of PAHs with pyridine and the electrospray ionization are simultaneously activated by applying a high voltage onto the Pt electrode. Notably, the derivatization takes advantage of the inherent electrochemical aspect of electrospray ionization, that is, the electrooxidation of substrates in the positive-ion mode, thus requiring no extra electrochemical cell and potentiostat. Through the electrochemical derivatization of PAHs into the corresponding pyridinium ions, the ECAD-nESI-MS method achieved remarkably improved detection sensitivity, where the limit of detection (LOD) is 3~10 times lower than the state-of-the-art atmospheric pressure chemical ionization mass spectrometry (APCI-MS) and gas chromatography mass spectrometry (GC-MS). With advantages in low detection limit, wide linear range, fast analysis speed (< 3 min per sample), and low sample consumption, we anticipate the wide application of the ECAD-nESI-MS method in the rapid and sensitive analysis of PAHs and their analogues.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ag NPs loading and ultraviolet irradiation strategies for enhancing the performance of WO₃/Ti₃C₂T_X composites in detecting 2-heptanone.","authors":"Jinfu Zhang, Pei Li, Helei Yang, Zhiguo Zhu, Hua Zhou, Zhenhe Wang, Xia Sun","doi":"10.1007/s00216-025-06143-0","DOIUrl":"https://doi.org/10.1007/s00216-025-06143-0","url":null,"abstract":"<p><p>As a typical volatile organic compound (VOC), 2-heptanone detection is critical in food spoilage monitoring, chemical production safety control, and environmental pollutant tracking. However, developing a 2-heptanone gas sensor with high sensitivity at room temperature remains challenging. This study prepared WO₃/Ti₃C₂T_X MXene composites (WT-50) via a solvothermal method and constructed a ternary composite Ag/WO₃/Ti₃C₂T_X-50 (AgWT-50) by loading Ag nanoparticles. The gas-sensing properties of WT-50, AgWT-50, and WT-50 excited by ultraviolet light toward 2-heptanone were investigated. AgWT-50 exhibits a high response of 4.62 to 55.04 ppm 2-heptanone at room temperature, with excellent selectivity and stability. The UV-irradiated WT-50 (WT-50Z) demonstrates more significant performance enhancement, with a response of 6.54, which is twice that of WT-50 (3.25), and notably improved selectivity. Mechanistic studies reveal that the performance improvements of AgWT-50 and WT-50Z originate from increased oxygen vacancies induced by Ag nanoparticle loading and UV-induced high-energy hot electrons and elevated photogenerated carrier concentration, respectively. Finally, the fabricated sensor was successfully applied to evaluate the freshness of salmon, establishing a food quality monitoring model based on gas-sensing responses. This study provides a new strategy for developing room-temperature high-performance gas sensors, showing significant application potential in food quality control, public health protection, and environmental safety fields.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the formation of biogenic nanoparticles and their protein corona: State-of-the-art and analytical challenges.","authors":"Andrés Suárez Priede, Miguel Gómez-Sánchez, Paula García-Cancela, Jörg Bettmer, Paula Díez","doi":"10.1007/s00216-025-06144-z","DOIUrl":"https://doi.org/10.1007/s00216-025-06144-z","url":null,"abstract":"<p><p>Biogenic synthesis of nanoparticles (NPs) using biological organisms such as plants, fungi, and bacteria offers a sustainable, green alternative to conventional chemical synthesis. These green methods involve biological matrices mediating metal ion reduction to form NPs capped by complex biomolecular coronas. These protein coronas critically affect NP stability, biological identity, and functionality, including notable therapeutic potentials. Despite advancements, the dynamic and heterogeneous nature of in situ biogenic NP formation remains insufficiently understood, as most studies emphasize synthetic production using biological extracts combined with metal precursors. Moreover, characterization of biogenic NPs requires a suite of bioanalytical techniques, each offering diverse advantages but also inherent limitations, requiring a comprehensive understanding of their features to critically select the optimal combination of strategies to obtain a full descriptive picture of the studied NPs. This review critically discusses current biogenic synthesis methods alongside the complementary bioanalytical strategies employed to elucidate NP formation, corona composition, and their biological implications. By highlighting the strengths and weaknesses of available techniques, the review aims to identify key challenges and future directions necessary to advance the safe and effective development of green nanotechnologies.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel approach to determination of amino acids and cyclic heptapeptides using imprinted proteins.","authors":"Kirill Yu Presnyakov, Polina M Ilicheva, Ivan A Reshetnik, Mikhail V Pozharov, Pavel S Pidenko, Natalia A Burmistrova","doi":"10.1007/s00216-025-06103-8","DOIUrl":"https://doi.org/10.1007/s00216-025-06103-8","url":null,"abstract":"<p><p>For the first time, imprinted proteins (IPs) were used as molecular recognition elements for determination of amino acids (arginine, Arg) and heptapeptides (microcystin-LR, MC-LR) in competitive solid-phase fluorescent assay (CSPA). Bovine serum albumin and ovalbumin were used as protein matrices for imprinting procedures. Preliminary simulation of the interaction of Arg and proteins by molecular docking and dynamic methods was used to predict optimal template concentration during IP synthesis. We developed an original method of increasing the affinity of IP binding sites by imprinting the Arg derivative (Arg-aniline) with greater accessibility of template amino groups. Silica nanoparticles modified with IPs have a high sorption capacity to Arg (7.8 mg g <math><mmultiscripts><mrow></mrow> <mrow></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </mmultiscripts> </math> ). We have developed procedures for determination of Arg and MC-LR by using CSPA; the linear determination range for Arg is 0.7-10 ng mL <math><mmultiscripts><mrow></mrow> <mrow></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </mmultiscripts> </math> (LoQ = 0.5 ng mL <math><mmultiscripts><mrow></mrow> <mrow></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </mmultiscripts> </math> ), while for MC-LR it is 4-60 ng mL <math><mmultiscripts><mrow></mrow> <mrow></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </mmultiscripts> </math> (LoQ = 2 ng mL <math><mmultiscripts><mrow></mrow> <mrow></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </mmultiscripts> </math> ). The obtained analytical approach was tested for Arg determination in a biologically active supplement.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthocyanins extraction from Amazonian matrices using natural deep eutectic solvents: characterization and ecological impact.","authors":"Ianê Valente Pires, Antonio Manoel da Cruz Rodrigues, Carolina Turnes Pasini Deolindo, Rodrigo B Hoff, Luiza Helena Meller da Silva, Marleny D A Saldaña","doi":"10.1007/s00216-025-06100-x","DOIUrl":"https://doi.org/10.1007/s00216-025-06100-x","url":null,"abstract":"<p><p>This study investigated the effectiveness of natural deep eutectic solvents (NADES) as a green alternative to traditional organic solvents for the extraction of anthocyanins from three Amazonian matrices: açaí, mangosteen pericarp, and purple yam. High-intensity ultrasound-assisted extraction (HIUS) was evaluated by varying key processing parameters such as molar ratio, solid-to-liquid ratio (S/L), added water, extraction time, and ultrasound power. Results showed that both molar ratio and added water had a significant impact on extraction efficiency. The addition of water to NADES was crucial in reducing viscosity and enhancing anthocyanin solubility, thereby improving mass transfer. However, excessive water led to decreased extraction yields, likely due to the disruption of hydrogen bonds within the NADES system. The extraction time and ultrasound power proved to be critical factors, as prolonged extraction periods and high power resulted in the degradation of anthocyanins. This study concluded that NADES yielded promising results for anthocyanin extraction. The optimal anthocyanin extraction conditions for açaí (132.20 mg/100 g) were a 1:2 molar ratio, 1:120 S/L, 30% added water, 5 min extraction, and 600 W power. For mangosteen pericarp, the optimal anthocyanin extraction conditions (58.81 mg/100 g) were a 1:2 molar ratio, 1:120 S/L, 30% added water, 5 min, and 1200 W power. For purple yam, the optimal anthocyanin extraction conditions (25.95 mg/100 g) were a 1:3 molar ratio, 1:120 S/L, 20% added water, 5 min, and 1200 W power. Additionally, the NADES-extracted anthocyanins demonstrated good stability across a range of pH values. Liquid chromatography coupled with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS) analysis revealed variability in phenolic profiles, while açaí and mangosteen pericarp showed diverse bioactive compounds, including isorhamnetin and epicatechin. Purple yam exhibited no detectable phenolics. Compared to conventional solvents, NADES extraction exhibited a lower environmental impact, as assessed by Ecoscale and Analytical GREEnness Metric (AGREE) tools.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}