Analytical and Bioanalytical Chemistry最新文献

{"title":"Colorimetric discrimination of chrysanthemum polyphenols via tunable MOF-818 nanozyme sensor array.","authors":"Rui Dong, Li Jin, Jili Hu, Jianhua Shu, Chun Ma, Chengle Li, Hongxing Kan, Liping Sun","doi":"10.1007/s00216-026-06855-x","DOIUrl":"https://doi.org/10.1007/s00216-026-06855-x","url":null,"abstract":"<p><p>Chrysanthemum is an important botanical resource with medicinal and dietary value, whose quality and therapeutic efficacy are closely related to its polyphenolic composition. However, conventional polyphenol analysis is limited by high cost, time consumption, and operational complexity. To address these challenges, a colorimetric sensor array was constructed using two metal-organic framework nanozymes (MOF-818-1 and MOF-818-2). Although derived from identical precursors, modulated solvothermal synthesis endowed the nanozymes with distinct catecholase-like catalytic activities, enabling the oxidation of o-diphenols into colored o-quinones. Differential responses toward chlorogenic acid (CGA), quercetin (QR), and dicaffeoylquinic acid (DCQA) generated characteristic two-dimensional fingerprints. Combined with multivariate pattern recognition methods, including principal component analysis (PCA), linear discriminant analysis (LDA), partial least squares discriminant analysis (PLS-DA), and hierarchical cluster analysis (HCA), the sensor array enabled proof-of-concept discrimination and concentration estimation of target polyphenols. The method was successfully applied to real chrysanthemum extracts, providing a rapid, simple, cost-effective strategy for herbal quality control.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A topical collection honoring the lasting impact of Günter Gauglitz on analytical chemistry.","authors":"Antje J Baeumner","doi":"10.1007/s00216-026-06863-x","DOIUrl":"https://doi.org/10.1007/s00216-026-06863-x","url":null,"abstract":"","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthogonality-driven offline two-dimensional liquid chromatography coupled with high-resolution mass spectrometry for comprehensive chemical profiling and quality marker screening of Renshen Guben oral liquid.","authors":"Dong Wang, Mengrong Li, Xiangyu Zhang, Tianshu Liu, Liming Wang, Bingjie Zhu, Guimin Zhang","doi":"10.1007/s00216-026-06857-9","DOIUrl":"https://doi.org/10.1007/s00216-026-06857-9","url":null,"abstract":"<p><p>Traditional Chinese patent medicines present formidable analytical challenges due to their multi-herb composition, extreme polarity diversity, and vast concentration disparities among constituents, rendering conventional one-dimensional liquid chromatography-mass spectrometry (1D LC-MS) insufficient for comprehensive quality assessment. This study establishes an orthogonality-driven offline two-dimensional liquid chromatography system (HILIC × RPLC) coupled with Q-Orbitrap mass spectrometry for in-depth chemical profiling and rational quality marker (Q-marker) screening of Renshen Guben oral liquid (RSGB), a clinically used immunomodulatory formulation comprising ten herbal ingredients. The 2D-LC platform was systematically optimized and validated, achieving a ~50-fold improvement over 1D LC-MS in component coverage. Global natural products social molecular networking (GNPS) was employed to visualize MS/MS spectral similarity relationships, facilitating confident annotation of structurally related analogues across 13 chemical classes. A total of 260 compounds were identified, among which 48 were verified against reference standards. Building upon this comprehensive chemical foundation, a multidimensional Q-marker screening strategy was devised by integrating network pharmacology prediction, molecular docking evaluation against the core immune-related target HSP90AA1, and chromatographic fingerprint correlation of ten constituent herbs, ultimately designating 12 compounds from eight distinct herbs as Q-markers. To address the wide dynamic range in component abundance, an adjusted multiple reaction monitoring (MRM) method utilizing triple quadrupole mass spectrometry was developed, enabling simultaneous quantification spanning three orders of magnitude. Method validation demonstrated excellent linearity (R² > 0.999) and satisfactory precision and repeatability for all 12 Q-markers. Quantitative analysis of ten commercial batches revealed significant inter-batch variations, with gallic acid as the most abundant constituent and alisol B the least, underscoring the necessity of multicomponent quality control. This integrated workflow bridges the gap between comprehensive chemical characterization and pragmatic quantitative assessment, offering a transferable paradigm for holistic quality evaluation of complex botanical formulations.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming sensitivity limitations in lateral flow immunoassays for small-molecule detection: from tracer engineering to assay format innovation.","authors":"Xinyi Gao, Xiaoyi Chen, Mengxiang Su, Bin Di","doi":"10.1007/s00216-026-06830-6","DOIUrl":"https://doi.org/10.1007/s00216-026-06830-6","url":null,"abstract":"<p><p>Typical small-molecule contaminants such as mycotoxins, drug impurities, food additives, and antibiotics can enter living organisms through multiple pathways, which may cause a range of health problems. Timely detection of abnormalities in these small molecules is crucial for maintaining health. The lateral flow immunoassay (LFIA), as a simple, rapid, and intuitive detection method, allows for real-time analysis of samples and has become an indispensable tool for on-site screening. However, the sensitivity and robustness of traditional gold nanoparticles (AuNPs)-based LFIA remain insufficient for reliable detection in complex matrices, largely due to limitations in signal generation and assay architecture. This review critically analyzes the recent development of LFIA for small-molecule detection from two perspectives. Firstly, we focus on advances in assay format engineering, including oriented antigen-antibody coupling, heterologous coating approaches, and innovative non-competitive configurations, which collectively improve recognition efficiency and analytical performance. In addition, signal amplification strategies through tracer engineering are discussed, including surface modification, multivalent labeling strategies, and colorimetric- or fluorescence-based signal enhancement. For complex detection matrices, the incorporation of magnetic nanoparticles (MNPs) and surface-enhanced Raman scattering (SERS) has also been explored as an effective way to strengthen signal output. Finally, this article points out the technical limitations and future development trends, aiming to provide conceptual guidance for the rational design of next-generation LFIA.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and correction of charcoal-stripped serum matrix bias in stable-isotope-dilution LC-MS/MS quantification of endogenous serum folates.","authors":"Shuangshuang Zhu, Yik Lam Cho, Ying Deng, Meng Wei, Chan Weng Huen, Chui Mae Wong, Joo Guan Yeo, Xueming Dong","doi":"10.1007/s00216-026-06848-w","DOIUrl":"https://doi.org/10.1007/s00216-026-06848-w","url":null,"abstract":"<p><p>Isotope-dilution LC-MS/MS is widely used for targeted quantification of endogenous metabolites, but surrogate-matrix calibration can remain biased when the surrogate matrix is noncommutable with native specimens. In this study, three endogenous serum folate vitamers were quantified by stable-isotope-dilution LC-MS/MS using charcoal-stripped serum (CSS) as a surrogate blank matrix. Post-extraction spiking and pooled-serum standard addition showed analyte-dependent and batch-dependent matrix bias between CSS and native serum. Standard addition comparison revealed matrix factors of 1.005 to 1.611 for 5-methyltetrahydrofolate (5-mTHF), 0.607 to 1.000 for folic acid (FA), and 0.902 to 1.008 for folinic acid, indicating a higher analyte-to-internal-standard normalized response in native serum relative to CSS for 5-mTHF, a lower normalized response for FA, and minimal matrix-dependent response difference for folinic acid. To address this bias, we developed a pooled-serum, batch-specific matrix-factor correction workflow based on standard addition. This approach estimates matrix factors in pooled native serum and applies them to CSS-calibrated concentrations generated under the same batch conditions, avoiding the sample-volume burden of conventional standard addition. Matrix-factor correction reduced validation bias for 5-mTHF and FA to within the predefined ± 15% criterion. In 64 clinical serum samples, correction changed classification relative to selected total folate reference decision limits (5-21 ng/mL) in ten samples (15.6%) for 5-mTHF and five samples (7.8%) for FA. These findings show that CSS-based stable-isotope-dilution LC-MS/MS can produce residual matrix-dependent bias despite normalization using stable-isotope-labeled internal standards (SIL-IS), and that pooled-serum standard addition provides a practical correction strategy.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly method for the analysis of the soluble fraction of parabens leached from sludge of a hospital wastewater treatment plant-proof of concept.","authors":"S Bedoya Patiño, J P Arrubla Vélez, J M Grisales Bedoya, M Becerra-Herrera, P Richter, E Carasek, J J Ríos Acevedo, M Rosero-Moreano","doi":"10.1007/s00216-026-06762-1","DOIUrl":"https://doi.org/10.1007/s00216-026-06762-1","url":null,"abstract":"<p><p>Developing eco-friendly analytical methods is crucial for monitoring emerging pollutants in complex matrices. This study presents a methodology based on green chemistry for determining the bioavailable fraction of methylparaben (MeP), ethylparaben (EtP), propylparaben (PrP), and butylparaben (BuP) in hospital wastewater treatment plant sludge. The approach combines in situ leaching with rotating-disk sorptive extraction (RDSE) in reverse mode, followed by direct-immersion solid-phase microextraction (DI-SPME) and headspace (HS-SPME) with on-fiber derivatization. GC-MS analysis identified the DVB/CAR/PDMS fiber as the most effective due to its high analyte affinity. Derivatization was successfully performed at room temperature in 10 min, with mass spectral match factors above 85%. The active analytical workflow (SPME extraction, on-fiber silylation, and GC-MS analysis) is completed in under 60 min per sample, enabling high-throughput screening once the parallel overnight leaching is completed. The method shows high sensitivity (LODs: 2.1-5.2 ng/g), good reproducibility (RSD < 9%), and accuracy (recoveries: 80-96% at 800 ng/g, n = 3). The four parabens were quantified in real sludge samples; MeP, EtP, and BuP were detected below their limits of quantification (< LOQ), while PrP was quantified at 20.0 ± 2.4 ng/g. Significant matrix effects (50% signal reduction) necessitated a standard additions protocol. The method's greenness, practicality, and innovation were validated using AGREEprep (score 0.54), BAGI (score 75.0), and VIGI (score 75) metrics, confirming its high sustainability, routine feasibility, and innovative character for complex environmental sample preparation.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Making surface-functionalized nanoscale candidate reference and test materials for interlaboratory comparisons involving multi-method studies.","authors":"Isabella Tavernaro, Sarah-Luise Abram, Lena Scholtz, Maria Richter, Anna Matiushkina, Klas Meyer, Peter Sjövall, Mikael Sundin, Mikael Järn, Jernej Ekar, Janez Kovač, U Resch-Genger","doi":"10.1007/s00216-026-06854-y","DOIUrl":"https://doi.org/10.1007/s00216-026-06854-y","url":null,"abstract":"<p><p>The reliable characterization of engineered nanomaterials (NMs) and nano-enabled advanced materials (NAMs) requires validated analytical methods for measuring key properties such as size, size distribution, morphology, chemical composition, and surface chemistry. Establishing such methods and verifying instrument performance and measurement competence requires (certified) reference materials (CRMs/RMs), representative test materials (RTMs), reference data (RD), and interlaboratory comparisons (ILCs). Although several nanoscale size standards exist, no RMs/RTMs with well-defined surface chemistry are available, despite the critical role of surface functional groups (FGs), ligands, and coatings in NM functionality and safety. Here, we report the development and characterization of two surface-modified nanoscale candidate RTMs, SiO₂-50-NH₂ and SiO₂-80-NH₂, comprising nonporous silica nanoparticles with total amino FG amounts of 205.7 ± 4.8 and 103.2 ± 3.0 nmol mg⁻¹, respectively. Material selection, bottling, homogeneity, and stability were assessed using a previously established multi-method characterization strategy. Analytical techniques included dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), zeta potential measurements, electron microscopy (EM), an automated optical Fluram assay, and quantitative nuclear magnetic resonance spectroscopy (qNMR) for determining derivatizable and total amino FGs. The results highlight the importance of combining complementary analytical methods rather than relying on a single technique. Additionally, a set of aminated silica NMs with varying amino FG densities were used to calibrate X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS), enabling amino FG quantification and method correlation. This characterization concept represents a first step towards establishing RMs/RTMs for NM surface chemistry measurements and ILCs assessing suitable analytical methods and their performance.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stephen A. Wise: An outstanding scientific career catalysed by multifaceted cooperations.","authors":"Hendrik Emons","doi":"10.1007/s00216-026-06837-z","DOIUrl":"https://doi.org/10.1007/s00216-026-06837-z","url":null,"abstract":"","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble polymeric system reducing non-specific interactions in plasmonic biosensor-based detection of carcinoembryonic antigen.","authors":"Tomáš Špringer, Libor Kostka, Vladimír Šubr, Ludmila Jurgová, Tomáš Etrych, Jiří Homola","doi":"10.1007/s00216-026-06859-7","DOIUrl":"https://doi.org/10.1007/s00216-026-06859-7","url":null,"abstract":"<p><p>Non-specific interactions in detection systems are among the main factors limiting the performance of surface-sensitive biosensor technologies. Here, we report on a new macromolecular blocker based on low-fouling poly[N-2-(hydroxypropyl)methacrylamide] (HPMA) copolymer containing hydrophobic comonomer units, which can reduce non-specific interactions improving the limit of detection when used in conjunction with a sandwich assay employing functional gold nanoparticles (AuNPs). The poly(HPMA) blocker adopts a relatively compact or collapsed conformation in PBS buffer, potentially indicating significant intramolecular interactions in an extended random coil. In a model experiment, we use this approach with a surface plasmon resonance biosensor for the detection of carcinoembryonic antigen, a biomarker of colorectal cancer. We demonstrate that the poly(HPMA) blocker reduced the AuNP non-specific binding and provided a high reduction of the non-specific binding when compared to conventional additives such as bovine serum albumin (BSA) and Tween. A combination of poly(HPMA) blocker and BSA was found to be the most effective additive, providing a 2.9-fold decrease of AuNP non-specific sensor response and a 2.8-fold improvement of the limit of detection compared to BSA alone.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver doping induced fluorescence enhancement of gold nanoclusters combined with machine learning for detection of quercetin.","authors":"Yan Su, Pengfei Gao, Guomei Zhang, Ying Zhou, Jianlin Wu, Wenting Liang, Lihong Shi, Shaomin Shuang, Yan Zhang","doi":"10.1007/s00216-026-06856-w","DOIUrl":"https://doi.org/10.1007/s00216-026-06856-w","url":null,"abstract":"<p><p>A novel fluorescence sensing method of quercetin (Que) was constructed based on gold and silver bimetallic nanoclusters (L-Cys@Au-Ag NCs) that were prepared by sonochemical synthesis. In comparison with gold nanoclusters, the bimetallic nanoclusters produced enhanced fluorescence along with a blue-shift, which is achieved by Ag doping to engineer the aggregation states of the Au(I)-thiolate motifs in the NC shell. Since the fluorescence was quenched after the addition of Que, a rapid-response and highly selective method to detect Que has thereby been established using L-Cys@Au-Ag NCs. This analytical method exhibited good sensitivity in the range of 9.07-115 μM with a limit of detection (LOD) of 1.064 μM, and it was used for the quantitative analysis of quercetin in grape juice samples. Moreover, the accuracy and feasibility of the developed fluorescence sensing method were validated by combining machine learning and HPLC. This machine learning-assisted analytical method offered a promising tool for food safety monitoring and human health surveillance.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}