Sterol analysis in cancer cells using atmospheric pressure ionization techniques.

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Pia Wittenhofer, Juan F Ayala-Cabrera, Laila Orell, Florian Uteschil, Sven W Meckelmann, Oliver J Schmitz
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引用次数: 0

Abstract

The analysis of sterol biosynthesis poses analytical challenges such as vast concentration differences between precursor molecules and cholesterol as well as the substantial interferences between the sterols and the matrix, resulting in strong ion suppression. LC-MS and 2D-LC-MS may address some of these challenges, but for sensitive and robust detection, ionization plays a key role. Electrospray ionization (ESI) stands out for its capability to generate ions from polar and nonpolar compounds, rendering it suitable for a broad spectrum of analytes, including sterols. Atmospheric pressure chemical ionization (APCI) demonstrates efficacy for compounds exhibiting moderate to low polarity, thereby facilitating efficient ionization of sterols. Tube plasma ionization (TPI) is a relatively new technique that promises sensitive ionization across a wide polarity range, indicating its potential utility for sterol analysis. Here, a new tube plasma ion source for LC-MS was developed and compared with established ion sources (ESI and APCI) for sterol analysis. The TPI source showed comparable LOQs to APCI and clearly outperformed ESI in terms of sensitivity. Both TPI and APCI provided stable signals during extended measurement series, while ESI suffered from pronounced ion suppression. Application to human plasma, HepG2 cells, and liver tissue demonstrated that TPI provided results in close agreement with APCI, highlighting its robustness for sterol quantification in complex biological matrices.

用常压电离技术分析癌细胞中的甾醇。
对甾醇生物合成的分析提出了分析方面的挑战,如前体分子和胆固醇之间的巨大浓度差异,以及甾醇和基质之间的实质性干扰,导致强烈的离子抑制。LC-MS和2D-LC-MS可以解决其中的一些挑战,但对于敏感和稳健的检测,电离起着关键作用。电喷雾电离(ESI)因其从极性和非极性化合物中产生离子的能力而脱颖而出,使其适用于广泛的分析物,包括甾醇。大气压化学电离(APCI)对中低极性化合物有效,从而促进甾醇的有效电离。管等离子体电离(TPI)是一种相对较新的技术,有望在很宽的极性范围内实现敏感的电离,这表明了它在甾醇分析中的潜在效用。本研究开发了一种新的管状等离子体离子源,并与已有的用于甾醇分析的离子源(ESI和APCI)进行了比较。TPI源的loq与APCI相当,在灵敏度方面明显优于ESI。在延长的测量序列中,TPI和APCI都提供了稳定的信号,而ESI则受到明显的离子抑制。在人血浆、HepG2细胞和肝组织中的应用表明,TPI提供的结果与APCI非常一致,突出了其在复杂生物基质中甾醇定量的稳健性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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