Reports of Biochemistry and Molecular Biology最新文献_第2页

Mir-let-7a Differential Expression in Oral Squamous Cell Carcinoma and Oral Lichen Planus: Insights for Early Diagnostic Biomarker Development. Mir-let-7a在口腔鳞状细胞癌和口腔扁平苔藓中的差异表达：早期诊断生物标志物发展的见解

IF 1.2

Reports of Biochemistry and Molecular Biology Pub Date : 2025-01-01 DOI: 10.61186/rbmb.13.4.456

Seyedmovahed Seyedhoseini, Nooshin Mohtasham, Shadi Saghafi, Negin Alahkhani, Farnaz Mohajertehran, Fahimeh Afzaljavan, Mahammad Taghi Shakeri, Amirkian Bokharaei

{"title":"Mir-let-7a Differential Expression in Oral Squamous Cell Carcinoma and Oral Lichen Planus: Insights for Early Diagnostic Biomarker Development.","authors":"Seyedmovahed Seyedhoseini, Nooshin Mohtasham, Shadi Saghafi, Negin Alahkhani, Farnaz Mohajertehran, Fahimeh Afzaljavan, Mahammad Taghi Shakeri, Amirkian Bokharaei","doi":"10.61186/rbmb.13.4.456","DOIUrl":"10.61186/rbmb.13.4.456","url":null,"abstract":"Background: Early detection of oral squamous cell carcinoma (OSCC) is essential for improving treatment outcomes. Oral lichen planus (OLP) is recognized as a premalignant condition that may progress to OSCC. Recently, microRNAs, particularly miR-let-7a, have emerged as promising biomarkers for gene regulation and early disease diagnosis. This study aimed to evaluate the expression level of miR-let-7a in OSCC and OLP patients, and to compare it with healthy controls, to determine its potential as an early diagnostic marker.Methods: In this cross-sectional study, serum samples were collected from 36 OSCC patients, 38 OLP patients, and 38 healthy controls. Diagnosis of OSCC and OLP was confirmed via biopsy. Serum RNA was isolated, and after quality verification, cDNA was synthesized. Quantitative real-time PCR (qRT-PCR) was performed to assess miR-let-7a expression across the three groups. Statistical analysis was conducted using SPSS version 16.0.Results: Significant differences in miR-let-7a expression were observed among the groups. Mean expression levels of miR-let-7a were 1.55 ± 1.19 in OSCC, 2.97 ± 2.00 in OLP, and 7.02 ± 4.10 in the control group (p< 0.001). Lower miR-let-7a expression in OSCC was notably correlated with adverse clinicopathological features, including higher tumor grade (p < 0.001), advanced clinical stage (p= 0.011), larger tumor size (T2) (p< 0.0001), and lymph node involvement (p< 0.0001).Conclusions: The findings demonstrate that miR-let-7a expression is significantly reduced in OSCC and OLP patients compared to healthy controls, highlighting its potential as an early biomarker for detecting malignant transformation in oral lesions and understanding disease progression in OSCC and OLP.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 4","pages":"456-465"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Curcumin and Metformin Effects on the Kidney in a Model of Unilateral Nephrectomy and Ischemia-Reperfusion Injury. 姜黄素与二甲双胍对单侧肾切除术及缺血再灌注损伤模型肾脏影响的比较分析。

IF 1.2

Reports of Biochemistry and Molecular Biology Pub Date : 2025-01-01 DOI: 10.61186/rbmb.13.4.579

Samaneh Karimi, Farhad Koohpeyma, Layasadat Khorsandi, Armin Panahi, Fatemeh Rezaei-Tazangi, Fereshtesadat Fakhredini

{"title":"Comparative Analysis of Curcumin and Metformin Effects on the Kidney in a Model of Unilateral Nephrectomy and Ischemia-Reperfusion Injury.","authors":"Samaneh Karimi, Farhad Koohpeyma, Layasadat Khorsandi, Armin Panahi, Fatemeh Rezaei-Tazangi, Fereshtesadat Fakhredini","doi":"10.61186/rbmb.13.4.579","DOIUrl":"10.61186/rbmb.13.4.579","url":null,"abstract":"Background: Unilateral nephrectomy and renal ischemia-reperfusion injury (I/R) are causes of acute kidney injury that can cause renal dysfunction, decreased glomerular filtration rate, impaired homeostasis, and increased mortality. This study aimed to evaluate the combined effects of curcumin (Cur) and metformin (Met), both protective antioxidants, on renal tissue structure and Neutrophil gelatinase-associated lipocalin (Ngal) and Kidney injury molecule 1 (Kim-1) genes expression levels in rats undergoing unilateral nephrectomy and ischemia/reperfusion.Methods: In this experimental study, 40 male rats were randomly divided into five groups. The animals underwent unilateral nephrectomy and ischemia/reperfusion and were then treated with curcumin and metformin or both for 14 days. Subsequently, histopathological and morphometric analyses were performed, and Ngal and Kim-1 gene expression levels were evaluated using real-time PCR.Results: Histopathological damage, expression levels of blood urea nitrogen (BUN), creatinine (Cr), blood biochemical parameters and oxidative stress and expression levels of Ngal and Kim-1 genes were significantly reduced in the patient group that received metformin and curcumin simultaneously. However, total antioxidant capacity (TAC) was increased in the patient group that received curcumin + metformin. Morphometric parameters also improved in this group.Conclusions: The results showed that the combination therapy with curcumin and metformin effectively protected the kidneys against unilateral nephrectomy and I/R injury.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 4","pages":"579-589"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Alzheimer's Disease with Promoter Variations in NPY2R Gene. 阿尔茨海默病与NPY2R基因启动子变异的关系

IF 1.2

Reports of Biochemistry and Molecular Biology Pub Date : 2025-01-01 DOI: 10.61186/rbmb.13.4.507

Mohammad Reza Karimzadeh, Atefeh Najmadini, Sara Azhdari, Negin Zatalian, Fatemeh Zandi, Neda Salmani, Kolsoum Saeidi

{"title":"Association of Alzheimer's Disease with Promoter Variations in NPY2R Gene.","authors":"Mohammad Reza Karimzadeh, Atefeh Najmadini, Sara Azhdari, Negin Zatalian, Fatemeh Zandi, Neda Salmani, Kolsoum Saeidi","doi":"10.61186/rbmb.13.4.507","DOIUrl":"10.61186/rbmb.13.4.507","url":null,"abstract":"Background: Alzheimer's disease (AD) is a widespread neurodegenerative disorder among the elderly, characterized by dementia. The development of AD is significantly influenced by genetic risk factors.Methods: in this study, we have investigated the impact of rs2234759 and rs12507396 polymorphisms in the neuropeptide Y receptor Y2 (NPY2R) gene on AD. Nineteen AD patients and nineteen healthy controls were enlisted in our research. and the DNA samples of all participants were genotyped using amplification refractory mutation system-polymerase chain reaction (ARMS-PCR).Results: The results revealed a noteworthy association between rs2234759 and AD, with a noticeable difference observed in the frequency of genotypes and alleles of this polymorphism between patients and healthy controls (P< 0.001 for both). However, no significant difference was detected in the genotype distribution concerning the rs12507396 polymorphism between the two groups.Conclusions: Our findings provide compelling evidence of an association between the rs2234759 polymorphism in NPY2R and Alzheimer's disease. Given the significant role of NPY2R in brain tissue, this particular polymorphism may result in strengthened presynaptic inhibition of glutamate release.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 4","pages":"507-515"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects of Liposomal Vitamin C on SARS-CoV-2 Target Viral Entry Genes in Renal Cells. 脂质体维生素C对肾细胞中SARS-CoV-2靶病毒进入基因的保护作用

IF 1.2

Reports of Biochemistry and Molecular Biology Pub Date : 2025-01-01 DOI: 10.61186/rbmb.13.4.484

Abtin Behmardi, Leila Zolghadr, Farzad Rajaei, Saeideh Gholamzadeh Khoei, Nematollah Gheibi

{"title":"Protective Effects of Liposomal Vitamin C on SARS-CoV-2 Target Viral Entry Genes in Renal Cells.","authors":"Abtin Behmardi, Leila Zolghadr, Farzad Rajaei, Saeideh Gholamzadeh Khoei, Nematollah Gheibi","doi":"10.61186/rbmb.13.4.484","DOIUrl":"10.61186/rbmb.13.4.484","url":null,"abstract":"Background: The kidneys are a potential target for SARS-CoV-2 infection. Ascorbic acid (vitamin C) has been shown to play an important role in reducing the symptoms of SARS-CoV-2. Recently liposomal drug delivery platforms have demonstrated promising results in enhancing the effectiveness of various therapeutics including infectious diseases. In this study, we designed a liposomal delivery system containing vitamin C to evaluate its antiviral efficacy in COVID-19, focusing on its effects on viral entry gene expression in Vero cells.Methods: Vitamin C was loaded into a liposome made up of hydrogenated soybean phosphatidylcholine, cholesterol, and 1,2-distearoyl-sn-glycero-3 phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000], and their physicochemical properties were assessed. Next, the cytotoxicity of free and liposomal vitamin C on the survival of the Vero cell line was evaluated using the MTT assay. In addition, the expression of viral entry genes, angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2), key mediators of SARS-CoV-2 entry into kidney cells, was investigated using RTq-PCR.Results: Liposomes were successfully loaded with vitamin C, achieving an encapsulation efficiency of 88.03%. The liposomal vitamin C formulation exhibited a brilliant surface morphology as observed by SEM. Both free and liposomal forms of vitamin C showed cytotoxic effects at higher concentrations. Moreover, both forms downregulated the expression of viral entry genes, although the liposomal form showed superior inhibitory performance compared to the free form.Conclusions: The study suggests liposomal vitamin C as a safe, effective treatment for COVID-19 by targeting viral entry genes in kidney cells, protecting them from viral damage and inflammation.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 4","pages":"484-494"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Usefulness of A Random Spot Urine Proteins-to-Creatinine Ratio to Screen for Increased Albuminuria in Patients with Type 1 Diabetes. 随机点尿蛋白-肌酐比筛查1型糖尿病患者蛋白尿增加的有效性

IF 1.2

Reports of Biochemistry and Molecular Biology Pub Date : 2025-01-01 DOI: 10.61186/rbmb.13.4.561

El Hadji Malick Ndour, Rokhaya Dione, Fatou Gueye-Tall, Sokhna Mara, Gora Fall, Coumba Kamby, El Hadji Ousmane Sene, Moustapha Djite, Nene Oumou Kesso Barry, Pape Matar Kandji, Papa Madieye Gueye, Philomene Lopez-Sall, Aynina Cisse

{"title":"Usefulness of A Random Spot Urine Proteins-to-Creatinine Ratio to Screen for Increased Albuminuria in Patients with Type 1 Diabetes.","authors":"El Hadji Malick Ndour, Rokhaya Dione, Fatou Gueye-Tall, Sokhna Mara, Gora Fall, Coumba Kamby, El Hadji Ousmane Sene, Moustapha Djite, Nene Oumou Kesso Barry, Pape Matar Kandji, Papa Madieye Gueye, Philomene Lopez-Sall, Aynina Cisse","doi":"10.61186/rbmb.13.4.561","DOIUrl":"10.61186/rbmb.13.4.561","url":null,"abstract":"Background: Moderately increased albuminuria is a biomarker for early onset diabetic nephropathy. The aim of this study was to evaluate the performance of use proteinuria-to-creatininuria ratio (UPCR) at different cut-off to screen for increased albuminuria using albuminuria-to-creatininuria ratio (UACR) as a gold standard.Methods: This was a cross-sectional study. A random spot urine sample was collected from patients with type 1 diabetes to measure albuminuria and total proteinuria using respectively an immunoturbidimetric and a colorimetric assay. Albuminuria was expressed as UACR and proteinuria as UPCR. The area under the curve (AUC) method and the kappa coefficient were used to compare UPCR and UACR.Results: In 150 diabetic patients, moderately increased albuminuria was detected in 33.3% using UACR and 35.3% using UPCR at 272 mg/g. UPCR thresholds of 130, 150, 180 and 200 mg/g yielded higher detection rates than UACR. However, all UPCR cut-offs showed low diagnostic accuracy (AUC < 70%), and agreement with UACR was mild (kappa < 0.40).Conclusions: The level of agreement between UPCR and UACR was moderate. It is not sufficient for UPCR to replace UACR to screen for increased albuminuria in patient with type 1 diabetes.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 4","pages":"561-569"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

hsa_circ_0004121 and hsa_circ_0030162 Differentially Expressed in Plasma of Patients with Recurrent Implantation Failure. hsa_circ_0004121和hsa_circ_0030162在反复植入失败患者血浆中的差异表达

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-10-01 DOI: 10.61186/rbmb.13.3.428

Majid Zaki-Dizaji, Mostafa Saeedinia, Parisa Derogar, Behnoosh Jamshidi, Masoumeh Masoumi, Zohreh Heidary

{"title":"hsa_circ_0004121 and hsa_circ_0030162 Differentially Expressed in Plasma of Patients with Recurrent Implantation Failure.","authors":"Majid Zaki-Dizaji, Mostafa Saeedinia, Parisa Derogar, Behnoosh Jamshidi, Masoumeh Masoumi, Zohreh Heidary","doi":"10.61186/rbmb.13.3.428","DOIUrl":"https://doi.org/10.61186/rbmb.13.3.428","url":null,"abstract":"Background: Recurrent or repeated implantation failure (RIF) is a significant challenge that hampers the success rate of assisted reproductive technology (ART) in achieving pregnancy. The underlying mechanisms of RIF remain unclear. Recent studies have identified distinct expression patterns of circular RNAs (circRNAs) in the endometrial tissues of individuals with RIF. The objective of this research is to evaluate the expression of six candidate circRNAs in the plasma of RIF patients.Methods: The study included a total of sixty participants, comprising 30 RIF patients and 30 age-matched controls. Specific primers were designed for six circRNAs (hsa_circ_0001713, hsa_circ_0004121, hsa_circ_0030162, hsa_circ_0034642, hsa_circ_0034762, and hsa_circ_0092337), and expression analysis was carried out by RT-qPCR. Blood samples were collected from individuals during the implantation window, and cell-free RNA was extracted from plasma. Statistical analysis was performed using Graphpad Prism software.Results: Control and RIF groups had mean ages of 34.68 ± 6.2 and 36.80 ± 3.8 years, respectively. Plasma from RIF patients showed significant downregulation of hsa_circ_0030162 (p=0.02) and upregulation of hsa_circ_0004121 (p=0.003) compared to controls. Bioinformatic analysis predicted hsa-miR-125a-3p and hsa-miR-125a-5p as potential targets of hsa_circ_0030162 and hsa_circ_0004121, respectively.Conclusions: This study demonstrates the differential plasma expression of hsa_circ_0030162 and hsa_circ_0004121 in RIF patients, consistent their expression in endometrial tissue. These circRNAs may contribute to RIF pathogenesis. Further research is needed to validate these findings and explore their clinical utility.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 3","pages":"428-437"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HMGB1 Modulates Angiogenic Imbalance and Cardiovascular Complications in Preeclampsia through Decorin and VEGF Regulation. HMGB1通过Decorin和VEGF调节子痫前期血管生成失衡和心血管并发症。

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-10-01 DOI: 10.61186/rbmb.13.3.385

Huma Quasimi, Sheema Wazib, Gausal Azam Khan, Md Iqbal Alam

{"title":"HMGB1 Modulates Angiogenic Imbalance and Cardiovascular Complications in Preeclampsia through Decorin and VEGF Regulation.","authors":"Huma Quasimi, Sheema Wazib, Gausal Azam Khan, Md Iqbal Alam","doi":"10.61186/rbmb.13.3.385","DOIUrl":"https://doi.org/10.61186/rbmb.13.3.385","url":null,"abstract":"Background: Preeclampsia (PE) is a serious multisystem disorder that ranks among the leading causes of maternal and neonatal morbidity and mortality. The condition is characterized by an angiogenic imbalance, which has adverse effects on fetal development and contributes to an increased risk of cardiovascular disease in the long term. This study aims to explore the connection between sterile inflammation mediated by HMGB1 and angiogenic imbalance in PE by examining key markers such as HMGB1, VEGF, Decorin, and TGF-β.Methods: In an animal model of PE, we measured the levels of HMGB1, VEGF, Decorin, and TGF-β in plasma, placenta, and heart tissues using ELISA. Additionally, Decorin levels were assessed through immunofluorescence in trophoblasts.Results: We found that levels of Decorin and TGF-β were significantly elevated in the plasma, placenta, and heart tissues of PE animals compared to non-pregnant and pregnant controls, whereas VEGF levels were reduced. Treatment with Glycyrrhizic acid (GA) restored the expression levels of these markers to more normalized values in the PE groups.Conclusions: Our findings indicate that HMGB1 plays a critical role in preeclampsia by mediating the upregulation of anti-angiogenic factors like Decorin and the downregulation of angiogenic factors like VEGF. This study highlights a significant correlation between HMGB1 and Decorin in driving the angiogenic imbalance that contributes to the pathophysiology of PE.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 3","pages":"385-393"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-Derived circRNAs hsa_circ_0004771 and hsa_circ_0019120 Differentially Expressed in Colorectal Cancer and Polyps. 血小板来源的circRNAs hsa_circ_0004771和hsa_circ_0019120在结直肠癌和息肉中的差异表达

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-10-01 DOI: 10.61186/rbmb.13.3.368

Hossein Razzaghi, Mohammad Heiat, Ahmad Khoncheh, Mohammad Ali Abyazi, Majid Zaki-Dizaji

{"title":"Platelet-Derived circRNAs hsa_circ_0004771 and hsa_circ_0019120 Differentially Expressed in Colorectal Cancer and Polyps.","authors":"Hossein Razzaghi, Mohammad Heiat, Ahmad Khoncheh, Mohammad Ali Abyazi, Majid Zaki-Dizaji","doi":"10.61186/rbmb.13.3.368","DOIUrl":"https://doi.org/10.61186/rbmb.13.3.368","url":null,"abstract":"Background: Colorectal cancer (CRC) is the second most common cause of cancer-related deaths worldwide. Early detection is crucial for improving survival rates. Liquid biopsies, specifically analyzing circulating tumor-educated platelets (TEPs), have emerged as a promising tool for early CRC detection and monitoring treatment efficacy. This study investigated the expression levels of two specific circRNAs, hsa_circ_0004771 and hsa_circ_0019120, in the platelets of patients with CRC, advanced polyps, and healthy controls.Methods: Blood samples were obtained from 25 individuals with CRC, 25 individuals with advanced polyps, and 25 healthy controls. Platelet-derived total RNA was extracted, and expression analysis was conducted using reverse transcription quantitative PCR (RT-qPCR). Differential expression and receiver operating characteristic (ROC) curve analysis were performed using GraphPad Prism.Results: Both circRNAs were found to be upregulated in platelets from individuals with advanced polyps and CRC compared to healthy individuals. However, the upregulation was statistically significant only for hsa_circ_0004771 in CRC patients (p-value = 0.0036) and for hsa_circ_0019120 in both advanced polyp (p-value = 0.0175) and CRC patients (p-value = 0.0356). The combined analysis of both circRNAs achieved an area under the curve (AUC) of 0.8348 (95% CI: 0.7131 to 0.9565) with a sensitivity of 84% and specificity of 80% (p-value = 0.0002).Conclusions: This study showed that hsa_circ_0004771 and hsa_circ_0019120 dysregulated in both CRC and polyps and have potential as a novel diagnostic biomarker of CRC.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 3","pages":"368-376"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Curcumin on the Activity of MMP-17 and MMP-24 in Hepatocytes of Mice Exposed to Thioacetamide. 姜黄素对硫乙酰胺暴露小鼠肝细胞MMP-17和MMP-24活性的影响。

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-10-01 DOI: 10.61186/rbmb.13.3.329

Sahar Farzaneh, Masoud Salehipour, Farzaneh Tafvizi, Vahid Naseh

{"title":"The Effect of Curcumin on the Activity of MMP-17 and MMP-24 in Hepatocytes of Mice Exposed to Thioacetamide.","authors":"Sahar Farzaneh, Masoud Salehipour, Farzaneh Tafvizi, Vahid Naseh","doi":"10.61186/rbmb.13.3.329","DOIUrl":"https://doi.org/10.61186/rbmb.13.3.329","url":null,"abstract":"Background: Hepatocellular carcinoma is the most primitive form of liver cancer, which is related to chemo carcinogens such as thioacetamide (TAA) and tissue remodeling molecules such as Matrix metalloproteinases (MMPs). Antioxidants, like curcumin (Cur), can inhibit these factors. In this research, the effect of curcumin on the expression and activity of two MMP enzymes, MMP-14 and MMP-17, which are involved in the carcinogenesis of mice after chronic exposure to thioacetamide, is investigated.Methods: In this study, 30 mice were divided into six groups and studied for 4 months. The first group, control; the second group, curcumin; the third group, TAA; the fourth group, TAA and curcumin simultaneously; the fifth group, first treated with TAA for 2 months and then curcumin; and finally, the sixth group, first treated with curcumin for 2 months and then TAA. Afterward, the mice were euthanized, and their liver tissues were transferred to the laboratory for analysis of gene and protein expression.Results: The averages of gene expression were calculated using SigmaPlot software and showed that the expression of MMP-17 and MMP-24 genes and the levels of their proteins were significantly increased by thioacetamide (****p < 0001) compared to the control group. Pathological observations indicated necrosis and dysplastic foci in the TAA group.Conclusions: Considering the crucial roles of MMPs in various diseases, including hepatocellular carcinoma, the regulation of their gene expression and enzymatic activity is significant in preventing tumor progression. Compounds such as thioacetamide and polyphenols like curcumin can modulate the activity of MMP-17 and MMP-24.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 3","pages":"329-340"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144054326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zinc Mitigates MDMA-Prompted Apoptosis by Alteration of Cross Talk Among Bcl-2, Bax and P53 Genes Expression in Mouse Sertoli and Leydig Cell Lines. 锌通过改变小鼠支持细胞和间质细胞Bcl-2、Bax和P53基因表达的串扰减轻mdma诱导的细胞凋亡

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-10-01 DOI: 10.61186/rbmb.13.3.420

Salar Mahmoudi-Nejad, Isa Abdirad, Morteza Bagheri

{"title":"Zinc Mitigates MDMA-Prompted Apoptosis by Alteration of Cross Talk Among Bcl-2, Bax and P53 Genes Expression in Mouse Sertoli and Leydig Cell Lines.","authors":"Salar Mahmoudi-Nejad, Isa Abdirad, Morteza Bagheri","doi":"10.61186/rbmb.13.3.420","DOIUrl":"https://doi.org/10.61186/rbmb.13.3.420","url":null,"abstract":"Background: 3,4-methylenedioxymethamphetamine (MDMA) affects the male reproductive system. We investigated the mRNA levels of bax, bcl-2, and p53 genes in MDMA-induced apoptosis in mouse Sertoli (TM4) and Leydig (TM3) cells before and after taking Zinc.Methods: The TM3 and TM4 cells were cultured in four groups: I (untreated medium), II (medium with 5 mM MDMA), III (medium with 8 µM Zinc), and IV (medium with 8 µM Zinc prior to 5 mM MDMA administration). After 48 hours, total RNA was extracted from the samples, and cDNA was synthesized. The relative gene expression level was evaluated using the SYBR Green PCR kit.Results: In the MDMA group, the relative amounts of bax and p53 gene expressions increased; conversely, the relative amount of bcl-2 gene expression decreased in TM3 and TM4 cell lines. In the MDMA+Zinc group, there were no statistically significant differences between this group and the control group regarding the mRNA levels of bax and p53 genes in the TM3 cell line, as well as the mRNA levels of bax and bcl-2 genes in the TM4 cell line. Statistically significant differences were observed between the MDMA+Zinc and MDMA groups regarding the relative expressions of bax and p53 genes in the TM3 cell line and bcl-2 and p53 genes in the TM4 cell line.Conclusions: Zinc mitigates MDMA- induced apoptosis by altering the crosstalk among bcl-2, bax, and p53 gene expressions in the tested cell lines.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 3","pages":"420-427"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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