Reports of Biochemistry and Molecular Biology最新文献_第3页

Catechin Hydrate Improves Hypertrophic Scar in Rabbit Ear Model via Reduction of Collagen Synthesis. 儿茶素水合物通过减少胶原蛋白合成改善兔耳模型中的肥厚性疤痕

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI: 10.61186/rbmb.13.1.13

Sina Goudarzi Afshar, Pari Tamri, Alireza Nourian, Ayoub Moahmoudi

{"title":"Catechin Hydrate Improves Hypertrophic Scar in Rabbit Ear Model via Reduction of Collagen Synthesis.","authors":"Sina Goudarzi Afshar, Pari Tamri, Alireza Nourian, Ayoub Moahmoudi","doi":"10.61186/rbmb.13.1.13","DOIUrl":"10.61186/rbmb.13.1.13","url":null,"abstract":"Background: Hypertrophic scar (HS) is a cutaneous condition results from abnormal wound healing following deep tissue injury. To date, there is no optimal treatment for this skin disorder. Catechins possess anti-inflammatory, antioxidant and anti-fibrotic properties. In this study we investigated the effects of catechin hydrate (CH) in rabbit ear model of HS.Methods: A rabbit ear model of hypertrophic scar was set up. Ten New Zealand white rabbit were divided into 5 equal groups: non-treatment group, vehicle control, treated with intralesional injection of dimethyl sulfoxide (DMSO), and test groups, received intralesional injection of CH/DMSO solution at concentration of 0.25, 1.25 and, 2.5 mg/ml, respectively. The treatments were initiated ‏‎35 days after wounding once a ‏week for ‏‎4 ‎weeks‎.‎ ‏ The scar elevation index (SEI) and the epidermal thickness index (ETI) were measured using Hematoxylin and Eosin (H & E) ‏staining and the amount of collagen deposition were determined after Masson‎' ‎trichrome staining‎. ‎In addition‎, the enzyme-linked immunosorbent assay (ELISA) ‎‏method was used to determine the levels of ‎ type І ‏and ІІІ collagen and matrix metalloproteinase ‏‎1 (MMP1) in scar tissues.Results: CH improved abnormal scarring at concentrations of 1.25 and 2.5 mg/ml and significantly (P<0.001) reduced the SEI and ETI. The levels of collagen type І and type ІІІ, and total collagen deposition were significantly (P<0.05) decreased in scar tissues of CH treated groups and no significant effect on MMP1 levels.Conclusions: Our findings demonstrated that CH has the potential for the treatment of HSs.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 1","pages":"13-22"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Body Mass Index with Matrix Metalloproteinase-9, Tissue Inhibitor of Metalloproteinase-1, and Interleukin-6 Based on Blood Pressure. 基于血压的体重指数与基质金属蛋白酶-9、组织金属蛋白酶抑制剂-1 和白细胞介素-6 的关系。

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI: 10.61186/rbmb.13.1.137

Tjam Diana Samara, Magdalena Wartono, Adrianus Kosasih, Alvina Alvina

{"title":"Association of Body Mass Index with Matrix Metalloproteinase-9, Tissue Inhibitor of Metalloproteinase-1, and Interleukin-6 Based on Blood Pressure.","authors":"Tjam Diana Samara, Magdalena Wartono, Adrianus Kosasih, Alvina Alvina","doi":"10.61186/rbmb.13.1.137","DOIUrl":"10.61186/rbmb.13.1.137","url":null,"abstract":"Background: A high body mass index (BMI) is known to be associated with high blood pressure. Levels of matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1), and interleukin-6 (IL-6) are also increased in hypertensive patients. The aim of this study was to determine the correlation of BMI with MMP-9, TIMP-1, and IL-6 based on blood pressure.Methods: The study design was cross-sectional with subjects aged >= 36 years, male and female and divided into 3 groups: group with normal blood pressure (NBP), group with controlled hypertension (CHT), and group with uncontrolled hypertension (UcHT). Height, weight, and blood pressure were measured, as well as serum levels of MMP-9, TIMP-1 and IL-6 using the ELISA method. The correlation was considered significant at p-value of < 0.05.Results: The BMI in group UcHT was higher than in the other groups. There was a positive correlation between BMI and MMP-9; BMI and TIMP-1; and BMI and IL-6 (r=0.480, p=0.007; r=0.620; p=0.000; r=374, p=0.042 respectively) in group UcHT.Conclusions: An increase in BMI is accompanied by an increase in levels of MMP-9, TIMP-1, and IL-6 in group UcHT, signifying that it is necessary to control BMI to maintain stable levels of MMP-9, TIMP-1, and IL-6, thereby keeping blood pressure under control.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 1","pages":"137-143"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining the Reference Range of Amino Acids in Healthy Neonatal Blood Samples in Northeast Iran Using LC-MS/MS. 利用 LC-MS/MS 确定伊朗东北部健康新生儿血液样本中氨基酸的参考范围

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI: 10.61186/rbmb.13.1.87

Zeinab Saeed, Baratali Mashkani, Amin Alaei, Abdolreza Varasteh, Fatemeh Keyfi

{"title":"Determining the Reference Range of Amino Acids in Healthy Neonatal Blood Samples in Northeast Iran Using LC-MS/MS.","authors":"Zeinab Saeed, Baratali Mashkani, Amin Alaei, Abdolreza Varasteh, Fatemeh Keyfi","doi":"10.61186/rbmb.13.1.87","DOIUrl":"10.61186/rbmb.13.1.87","url":null,"abstract":"Background: Amino acid analysis is an important tool for the diagnosis of metabolic disorders in newborns. Today, Liquid Chromatography tandem mass spectrometry (LC-MS/MS) has emerged as a powerful technique for amino acid analysis. We aimed to determine the local normal range of amino acids in dried blood spot (DBS) samples of neonates using LC-MS/MS.Methods: A total of 1005 samples from healthy neonates of northeast and east of Iran aged 2-7 days were utilized for normal range determination. The amino acids were extracted from dried blood spot samples using organic solvent and then analyzed using LC-MS/MS system. The 1%, 2.5%, 97.5%, and 99% percentiles were calculated, and the results were compared to the global cut-off values.Results: The results showed that glutamic acid has the highest concentration range among amino acids evaluated in this study (178.94 - 421.31µmol/L). Moreover, the plasma concentrations of Glycine (142.65 - 397.06 µmol/L), Alanine (97.00-349.72 µmol/L), Proline (63.77 - 236.53 µmol/L), and Tyrosine (25.79 - 150.58 µmol/L) were in the next ranks. Comparing the obtained results with the global values obtained in the R4S study indicated a slight difference between the obtained local normal values and the global values.Conclusions: The calculated values were slightly different from global values obtained in the R4S study and regional values calculated in other studies. This further emphasized the importance of the local establishment of reference values, which facilitates the correct interpretation and diagnosis in the Newborn Screening Programs.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 1","pages":"87-98"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression Levels of miR -124a, miR-545-3p and BDNF in the Peripheral Blood Mononuclear Cells Are Associated with the Severity of Autism. 外周血单核细胞中 miR -124a、miR-545-3p 和 BDNF 的表达水平与自闭症严重程度有关。

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI: 10.61186/rbmb.13.1.1

Safa Jasim Tuama Ali, Mohammad Khalaj-Kondori, Mohammad Ali Hosseinpour Feizi, Mehdi Haghi

{"title":"Expression Levels of miR -124a, miR-545-3p and BDNF in the Peripheral Blood Mononuclear Cells Are Associated with the Severity of Autism.","authors":"Safa Jasim Tuama Ali, Mohammad Khalaj-Kondori, Mohammad Ali Hosseinpour Feizi, Mehdi Haghi","doi":"10.61186/rbmb.13.1.1","DOIUrl":"10.61186/rbmb.13.1.1","url":null,"abstract":"Background: People with autism frequently exhibit poor social skills, communication difficulties, and repetitive and stereotyped behaviors. MicroRNAs (miRNAs) are potential and promised targets in developing of new treatment strategies for autism. This study aimed to assess the relative expression of miR-124a, miR-34a-3p, miR-545-3p, miR-153, and BDNF in the blood samples of autistic children.Methods: The children autism rating scale (CARS) was used to determine the severity of autism and to confirm the diagnosis. Blood samples were obtained from 50 patients and 40 age-/sex-matched healthy controls. Expressions of miR-545-3p, miR-34a-3p, miR-124a, and BDNF were evaluated using qRT-PCR. Pearson's correlation coefficient and regression analysis were used to check correlations between relative expressions of the miRNAs and BDNF. Biomarker potencies were assessed by ROC curve analysis.Results: qRT-PCR analysis showed that the relative expressions of miR-545-3p, miR-34a-3p, miR-124a, and BDNF were significantly higher in the patients' group than the healthy controls. However, the relative expression of miR-153 was significantly lower in the case group than the control group. The relative expression of miR-124a was positively correlated with those of miR-545-3p and BDNF among the patients group. Also, the relative expressions of miR-545-3p and BDNF were positively correlated with each other. The ROC curve data also indicated that miR-124a, miR-34a-3p, miR-545-3p, miR-153, and BDNF could be possible diagnostic biomarker for CARS diagnosis (AUC=0.8328, AUC=0.8354, AUC=0.6727, AUC=0.8518 and AUC=0.8214, respectively).Conclusions: Deregulation of miR-124a, miR-454-3p and BDNF might be considered as potential biomarkers for severity of autism.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 1","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Serum Interleukin-10 and Interleukin-27 Levels in the Prognosis of Immune Thrombocytopenia in Iraqi Children. 血清白细胞介素-10 和白细胞介素-27 水平在伊拉克儿童免疫性血小板减少症预后中的作用。

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI: 10.61186/rbmb.13.1.99

Huda Khudhyer Abbas, Basil Oied Saleh, Hasanein Habeeb Ghali

{"title":"Role of Serum Interleukin-10 and Interleukin-27 Levels in the Prognosis of Immune Thrombocytopenia in Iraqi Children.","authors":"Huda Khudhyer Abbas, Basil Oied Saleh, Hasanein Habeeb Ghali","doi":"10.61186/rbmb.13.1.99","DOIUrl":"10.61186/rbmb.13.1.99","url":null,"abstract":"Background: Several studies provide evidence for a role of serum cytokines imbalance including IL-10 and IL-27 in immune thrombocytopenia pathogenesis and prognosis. The aim of this study was designed to investigate the role of serum levels of IL-10 and IL-27 in prognosis the efficiency of treatment in thrombocytopenic Iraqi children.Methods: This case controls study was carried out at Department of Biochemistry, College of Medicine, University of Baghdad, during the period from October 2023 to March 2024. It included 88 children, 63 children previously diagnosed with immune thrombocytopenia, and 25 apparently healthy children who served as control group. The included immune thrombocytopenic children were sub-grouped according to their treatment into three groups: Romiplostim group (group 1), Prednisolone group (group 2), Prednisolone and intravenous immunoglobulin (IVIG) or Prednisolone and mycophenolate group (group 3). Investigations included serum level measurements of IL-10 and IL-27 by using enzyme linked immunosorbent assay ELISA. Platelet count of each included children was measured by Huma Count 30 TS Human, Germany.Results: The mean (±SEM) values of serum IL-10 and IL-27 levels of immune thrombocytopenic children were insignificantly lower than that of controls. In addition, there was non- significant differences in serum levels of IL-10 and IL-27 among and between the three groups of patient children. The mean value of platelet count of patient children was significantly increased by all types of treatment in whole immune thrombocytopenic children (117.48±18.15*10^9/L).Conclusions: Measurement of serum IL-10 and IL-27 are helpful biomarker in prognosis of thrombocytopenia irrespective of type of treatment.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 1","pages":"99-105"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TH1/TH2 Cytokine Profile and Their Relationship with Hematological Parameters in Patients with Acute Limb Ischemia. 急性肢体缺血患者的 TH1/TH2 细胞因子谱及其与血液学参数的关系

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI: 10.61186/rbmb.13.1.31

Jamal Jalili Shahri, Mohammad Hadi Saeed Modaghegh, Hamid Tanzadehpanah, Motahare Ebrahimnejad, Hanie Mahaki

{"title":"TH1/TH2 Cytokine Profile and Their Relationship with Hematological Parameters in Patients with Acute Limb Ischemia.","authors":"Jamal Jalili Shahri, Mohammad Hadi Saeed Modaghegh, Hamid Tanzadehpanah, Motahare Ebrahimnejad, Hanie Mahaki","doi":"10.61186/rbmb.13.1.31","DOIUrl":"10.61186/rbmb.13.1.31","url":null,"abstract":"Background: The progression of acute limb ischemia (ALI) is being significantly influenced by changes in immune system function. The study aimed to determine the dominant immune cell responses (Th1 or Th2) in ALI patients by measuring serum levels of IL-4, IL-12, and IFN-γ. Previous studies indicate altered cytokine levels in cerebral ischemia, but there is no prior research on these cytokines in ALI patients.Methods: This study involved 34 patients with ALI and 34 healthy controls. Blood samples were analyzed for hematological factors such as erythrocyte sedimentation rate (ESR), white blood cell (WBC) count, red blood cell (RBC) count, platelet (Plt) count, hemoglobin (Hb), and hematocrit (HCT). The levels of serum cytokines IL-4, IL-12, and IFN-γ were measured in both patients and control subjects using enzyme-linked immunosorbent assay (ELISA). The statistical analyses were conducted using SPSS and GraphPad Prism.Results: The results showed that serum levels of IL-4 in ALI patients did not significantly differ from those in control groups. Acute limb ischemia exhibited significantly elevated levels of IL-12 and IFN-γ compared to healthy individuals. In addition, no correlation between the production of cytokines and the hematological parameters was found.Conclusions: Th1 responses are believed to play a role in the pathogenesis of ALI, but further research is needed to fully understand their exact role.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 1","pages":"31-39"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of lncRNA ANRIL rs10757278 A>G Variant, Tumor Size, Grading, Tumor Site, and Tumor Stage in Oral Squamous Cell Carcinoma Patients. 口腔鳞状细胞癌患者的 lncRNA ANRIL rs10757278 A>G 变异与肿瘤大小、分级、肿瘤部位和肿瘤分期的关系

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI: 10.61186/rbmb.13.1.59

Maryam Salah, Mostafa Rezaee, Mohamad Javad Fattahi, Abbas Ghaderi, Bijan Khademi, Mohammad Javad Mokhtari

{"title":"Association of lncRNA ANRIL rs10757278 A>G Variant, Tumor Size, Grading, Tumor Site, and Tumor Stage in Oral Squamous Cell Carcinoma Patients.","authors":"Maryam Salah, Mostafa Rezaee, Mohamad Javad Fattahi, Abbas Ghaderi, Bijan Khademi, Mohammad Javad Mokhtari","doi":"10.61186/rbmb.13.1.59","DOIUrl":"10.61186/rbmb.13.1.59","url":null,"abstract":"Background: Oral Squamous Cell Carcinoma (OSCC) is a pressing global health challenge. Long non-coding RNAs (lncRNAs) have emerged as pivotal regulators. Among these, the lncRNA ANRIL (antisense non-coding RNA in the INK4 locus) has a role in cancer progression. The aim of this study was to look into possible links between a certain genetic variant of lncRNA ANRIL, rs10757278 A/G, and OSCC risk and tumor features in the Iranian population.Methods: We conducted a case-control study, enrolling 101 OSCC patients and 115 healthy controls. We took out the genomic DNA and used the tetra-primer ARMS-PCR (tetra-primer amplification refractory mutation system-polymerase chain reaction) method to find the rs10757278 genotype. We evaluated the associations between genotypes and both OSCC susceptibility and various tumor characteristics.Results: Although we did not observe significant differences in allele and genotype frequencies between cases and controls, we revealed compelling associations between genotypes and tumor characteristics. Genotypes AG and GG were linked to smaller tumor sizes, while genotypes with at least one wild-type allele (A) were linked to well differentiated OSCC. Specific genotypes exhibited significant associations with tumor sites, with the tongue demonstrating the strongest correlation.Conclusions: The rs10757278 A/G variant did not show a direct link with OSCC risk, but its complex effect on tumor behavior suggests that it may play a bigger role in the development of OSCC. These findings open avenues for future investigations to uncover hidden genetic interactions, and potentially inform more targeted therapeutic strategies.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 1","pages":"59-66"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation the Effect of Supplements Containing Vitamin D and Trace Elements in Patients with Moderate to Severe Asthma. 评估维生素 D 和微量元素补充剂对中重度哮喘患者的影响。

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI: 10.61186/rbmb.13.1.40

Mansoor Ahoon, Reza Farid-Hosseini, Hooman Tehrani, Houshang Rafat-Panah, Hamid Ahanchian, Hadis Yousefzadeh, Seyed Abdolrahim Rezaee, Maral Barzegar Amini, Farahzad Jabbari Azad

{"title":"Evaluation the Effect of Supplements Containing Vitamin D and Trace Elements in Patients with Moderate to Severe Asthma.","authors":"Mansoor Ahoon, Reza Farid-Hosseini, Hooman Tehrani, Houshang Rafat-Panah, Hamid Ahanchian, Hadis Yousefzadeh, Seyed Abdolrahim Rezaee, Maral Barzegar Amini, Farahzad Jabbari Azad","doi":"10.61186/rbmb.13.1.40","DOIUrl":"10.61186/rbmb.13.1.40","url":null,"abstract":"Background: Asthma is a common and major allergic disease in the world. We aimed to investigate the impact of supplements with vitamin D, folic acid, selenium, zinc, and copper in patients with moderate to severe asthma.Methods: In this clinical trial study 70 patients above six years old with moderate to severe asthma, were divided into two groups, randomly; one group received daily Asmavit syrup, 10 ml (Asmavit, Vitabiotics Ltd, London, UK), and the other group received daily 1000 IU vitamin D3 drops (Asmavit, Vitabiotics Ltd, London, UK) for two months along with ordinary treatment for asthma. Clinical and physical examinations, immunological and biochemical tests were carried out for each patient before and after the treatment.Results: The mean age of patients was 39.9± 14.7 years old, and the mean disease duration was 8.8 ± 9.8 years. A significant increase in lung function, asthma control, and quality of life score tests was observed in both groups after the treatment (P< 0.05). There was no significant difference in cytokines expression levels before and after the treatment with vitamin D3 or Asmavit (P> 0.05). Serum levels of selenium and folic acid before treatment were correlated with disease severity, while post-treatment vitamin D levels significantly increased FEV1 (P> 0.05). Oxidative stress levels reduced in both groups, with greater reduction in the vitamin D group (P< 0.05).Conclusions: Supplements, particularly vitamin D, when combined with standard asthma treatment, may effectively improve clinical symptoms and enhance the quality of life for asthmatic patients.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 1","pages":"40-50"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-COV-2 ORF9b Dysregulate Fibrinogen and Albumin Genes in a Liver Cell Line. SARS-COV-2 ORF9b 对肝细胞系中的纤维蛋白原和白蛋白基因产生失调作用

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI: 10.61186/rbmb.13.1.51

Shirin Jalili, Seyed Mohammad Ali Hashemi, Jamal Sarvari

{"title":"SARS-COV-2 ORF9b Dysregulate Fibrinogen and Albumin Genes in a Liver Cell Line.","authors":"Shirin Jalili, Seyed Mohammad Ali Hashemi, Jamal Sarvari","doi":"10.61186/rbmb.13.1.51","DOIUrl":"10.61186/rbmb.13.1.51","url":null,"abstract":"Background: Individuals experiencing severe cases of Coronavirus Disease 2019 (COVID-19) exhibited elevated fibrinogen levels and decreased albumin levels, potentially linked to the presence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) proteins. Consequently, our study endeavors to examine the impact of SARS-CoV-2 ORF9b on the expression of fibrinogen and albumin genes within the Hep-G2 cell line.Methods: In this study, the Hep-G2 liver cell line was utilized alongside the plasmid pcDNA3.1 hyg+ containing ORF9b from the SARS-CoV-2 strain originating in Wuhan. Transfection procedures were executed, and the transfected cells were selected utilizing hygromycin B. Validation of ORF9b expression was conducted through SYBR green-based real-time PCR, and the expression of the Fibrinogen α (FGA), Fibrinogen β (FGB), Fibrinogen γ (FGG), and Albumin (ALB) genes was quantified using the same method.Results: The real-time PCR analysis revealed a significant upregulation of fibrinogen genes-α (P=0.03), β (P=0.02), and γ (P=0.029) in Hep-G2 cells containing ORF9b compared to control cells. Furthermore, the findings indicated a markedly lower expression level of albumin in Hep-G2 cells harboring ORF9b compared to the control cells (P=0.028).Conclusions: The findings suggest that SARS-CoV-2 ORF9b could potentially influence the course of SARS-CoV-2 infection by triggering the expression of α, β, and γ fibrinogen gene chains while suppressing the albumin gene. Further investigations are warranted to validate these observations across various SARS-CoV-2 strains exhibiting differing levels of pathogenicity.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 1","pages":"51-58"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of ABCB1(Rs10276036, C/T) Gene, IL-18, and TNFα as Risk Factors for Nephrotic Syndrome Incidence. ABCB1（Rs10276036，C/T）基因、IL-18 和 TNFα 与肾病综合征发病率风险因素的关系

IF 1.6

Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI: 10.61186/rbmb.13.1.67

Eglal Aly Hassan, Afaf Mohamed Elsaid, Ahmed Mahmoud El-Refaey, Mohammed Abou Elzahab, Magdy Mahfouz Youssef, Rehab Elmougy

{"title":"Association of ABCB1(Rs10276036, C/T) Gene, IL-18, and TNFα as Risk Factors for Nephrotic Syndrome Incidence.","authors":"Eglal Aly Hassan, Afaf Mohamed Elsaid, Ahmed Mahmoud El-Refaey, Mohammed Abou Elzahab, Magdy Mahfouz Youssef, Rehab Elmougy","doi":"10.61186/rbmb.13.1.67","DOIUrl":"10.61186/rbmb.13.1.67","url":null,"abstract":"Background: The most common cause of Nephrotic Syndrome (NS) in children is idiopathic NS, also called nephrosis. The most prominent clinical signs are hyperlipidemia, severe proteinuria, edema, swelling of body tissues, and an increased risk of infection. The object of this study was to examine the correlation of the ABCB1 gene (rs10276036, C > T), IL-18, and TNFα to the prevalence of NS among Egyptian children having NS.Methods: This study included 100 participants with NS and 100 healthy controls. To analyze the ABCB1 gene (rs10276036 C >T) variant PCR technique was used. IL-18 and TNF levels were estimated using Enzyme-Linked Immunosorbent Assay (ELISA).Results: Increased frequency of CT and TT genotypes of the ABCB1 gene (rs10276036 C / T) in NS patients compared to controls, with p-value = 0.001, OR = 2.270, CI = (1.550-3.327) for CT genotype and p-value = 0.001, OR = 5.070, CI = (2.463-10.438) for TT genotype. The frequencies of ABCB1 (rs10276036 C >T) genotypes were statistically significant in the dominant model (OR 2.560; p< 0.001) and in the recessive model OR, 3.231; p= 0.001). Significantly high levels of both IL-18 and TNFα were found in NS patients compared to controls.Conclusions: The ABCB1gene (rs10276036 C/T), IL-18, and TNFα are associated with the prevalence of NS in Egyptian children and might be considered as independent risk factors for its incidence.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 1","pages":"67-78"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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