Reports of Biochemistry and Molecular Biology最新文献

{"title":"3,4 Dihydroxyphenylethanol May Inhibit Metastasis in HepG2 Cells by Influencing the Expression of miR-21 and Genes Associated with Metastasis.","authors":"Mahdi Alaee, Gholamreza Shahsavari, Mohammad Yazdi, Maryam Hormozi","doi":"10.61186/rbmb.13.2.254","DOIUrl":"10.61186/rbmb.13.2.254","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) is one of the lethal malignancies with a poor prognosis due to metastatic complications. Matrix metalloproteinases (MMPs) and their inhibitors, tissue inhibitors of metalloproteinases (TIMPs), have an important role in metastasis. MicroRNA-21 (miR-21) is significantly overexpressed in nearly all types of human cancers, including HCC. Targeting miR-21 pharmacologically could be a promising therapeutic approach for HCC. 3,4-dihydroxyphenylethanol (DHPE), a phenolic phytochemical compound found in olive, has potent antioxidant and anticancer properties. This study aimed to investigate the effect of DHPE on the expression of miR-21 with genes associated with metastasis (MMP-2, MMP-9, TIMP-1, and TIMP-2) and their correlation with miR-21 in HepG2 cells.</p><p><strong>Methods: </strong>This experimental study had four groups, including a control, and three groups of treatment with different concentrations of DHPE (50, 100, and 150 µM) for 24 hours. The expression levels of genes were determined by RT-qPCR.</p><p><strong>Results: </strong>The results showed that the treatment of cells with DHPE significantly reduced the expression of miR-21, MMP-2, MMP-9, and TIMP-1 but increased TIMP-2 compared to the control group; additionally, there was a negative correlation between miR-21 and TIMP-2 but a positive correlation between miR-21 with MMP-2, MMP-9, and TIMP-1.</p><p><strong>Conclusions: </strong>The results showed that DHPE, likely by reducing the expression of miR-21, can increase TIMP-2 and reduce MMP-2, MMP-9, and TIMP-1 gene expression and may play a role in inhibiting cell migration in HepG2 cells.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 2","pages":"254-262"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin and Catechin Protects Leptin-Deficient Lep^ob/Ob Mice Against Alloxan-Induced Diabetes and Hepatotoxicity via Suppression of Oxidative Stress and Inflammation.","authors":"Mahdieh Sadat Badiee, Ali Vadizadeh, Maryam Salehcheh, Mehrnoosh Moosavi, Maryam Shirani, Fereshtesadat Fakhredini, Mohammad Javad Khodayar","doi":"10.61186/rbmb.13.2.184","DOIUrl":"10.61186/rbmb.13.2.184","url":null,"abstract":"<p><strong>Background: </strong>The study focuses on evaluating the combined effects of quercetin (QCT) and catechin (CAT), both plant-based antioxidants, on alloxan-induced liver toxicity and diabetes in leptin-deficient (Lep^ob/ob) mice. Diabetes is a metabolic disorder characterized by high blood glucose levels due to inadequate insulin secretion or insulin resistance.</p><p><strong>Methods: </strong>Thirty mice were divided into five groups of 6, including: normal control, diabetic control, diabetic mice treated with 150 mg/kg CAT, diabetic mice treated with 150 mg/kg QCT, and diabetic mice treated with 150 mg/kg CAT, and 150 mg/kg QCT for seven days. Mice were anesthetized after overnight fasting on the 8th day, and the blood sample was collected and the levels of antioxidants and pro-inflammatory factors in serum, and the expression of ADP-ribose polymerase (PARP) protein were measured, and histological studies were performed.</p><p><strong>Results: </strong>The results showed that diabetic mice receiving QCT and CAT showed lower liver enzymes, fasting blood sugar (FBS), blood urea nitrogen (BUN), creatinine (Cr), cholesterol, triglyceride, low-density lipoprotein (LDL), TNF-α, and thiobarbituric acid reactive substances (TBARS) levels and increased high-density lipoprotein (HDL), total thiol, catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in the liver compared to the ALLO group alone (P<0.001). The level of PARP protein significantly declined in the ALLO group compared to the control group.</p><p><strong>Conclusions: </strong>The findings of this study demonstrated that QCT, and CAT are reasonably effective in preventing hepatotoxicity and diabetes in mice.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 2","pages":"184-195"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> Differentiation of Endometrium Stem Cells into Cardiomyocytes: The Putative Effect of miR-17-5p, miR-26b-5p, miR-32-5p, and SMAD6.","authors":"Somayeh Saadat, Mahdi Noureddini, Behnaz Maleki, Naeim Ehtesham, Alireza Farrokhian, Javad Verdi, Ebrahim Cheraghi, Hossein Ghanbarian, Behrang Alani","doi":"10.61186/rbmb.13.2.243","DOIUrl":"10.61186/rbmb.13.2.243","url":null,"abstract":"<p><strong>Background: </strong>The important role of SMAD6 and several microRNAs (miRNAs), such as miR-17-5p, miR-26b-5p, and miR-32-5p, has been demonstrated in controlling the proliferation and differentiation of cardiomyocytes (CMs). Hence, this study was designed to assess the role of these regulatory factors in cardiac cell generation from human endometrium-derived mesenchymal stem cells (hEMSCs).</p><p><strong>Methods: </strong>To induce transdifferentiation into CMs, hEMSCs were treated with a cardiac-inducing medium containing 5-azacytidine and bFGF for 30 days. Immunofluorescence staining and qRT-PCR, respectively, were used to measure the protein levels of SMAD6 and the mRNA expression of SMAD6 and the three miRNAs every six days.</p><p><strong>Results: </strong>Our findings demonstrated the mesenchymal stem cell properties of hEMSCs and their ability to differentiate into various types of mesenchymal stem cells. The differentiated hEMSCs exhibited morphological features resembling CMs. During the induction period, the number of positive cells for SMAD6 protein and the expression level of miR-26b-5p increased and peaking on days 24 and 30, while the expression levels of miR-17-5p and miR-32-5p decreased. The Pearson correlation coefficients revealed that SMAD6 level is inversely correlated with miR-17-5p and miR-32-5p and directly correlated with miR-26b-5p.</p><p><strong>Conclusions: </strong>Our results indicate that miR-17-5p, miR-26b-5p, miR-32-5p, and SMAD6 are potentially involved in the molecular signaling pathways of transdifferentiation of hEMSCs to CMs.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 2","pages":"243-253"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparison of Apelin Rs56204867 and Apelin Receptor Rs11544374 Gene Polymorphisms and Their Association with Risk of Preeclampsia in Southeast Iran.","authors":"Maryam Juybar, Mansour Shahraki, Marzieh Ghasemi, Abolfazl Payandeh, Shaghayegh Saljooghi, Mohsen Saravani","doi":"10.61186/rbmb.13.2.273","DOIUrl":"10.61186/rbmb.13.2.273","url":null,"abstract":"<p><strong>Background: </strong>Pre-eclampsia (PE) is a severe pregnancy condition with genetic and environmental factors affecting the placental function and vascular changes. Genetic variants in the apelinergic system may influence preeclampsia risk and birth outcomes. Therefore, this study aimed to compare apelin (APLN) rs56204867 and apelin receptor (APLNR) rs11544374 gene polymorphisms and to investigate their association with mothers' body mass index and infant's birth weight among women with preeclampsia and control group in southeast Iran.</p><p><strong>Methods: </strong>A total of 123 PE patients and 125 age- and gender-matched control subjects were enrolled in the study. The PCR-RFLP method was employed to genotype the APLN rs56204867 and APLNR rs11544374 gene polymorphisms.</p><p><strong>Results: </strong>There was no significant association between the genotypes of the rs11544374 variant and the PE risk. The incidence of the AG genotype of the rs54204867 variant in the control group was considerably greater than in the PE group. Also, a significant relationship was found between the body mass profile of patients with PE and the APLN rs54204867 gene polymorphism.</p><p><strong>Conclusions: </strong>It was observed that the APLN rs54204867 gene polymorphism could affect the PE risk. No significant difference was found between the PE group and the control group in terms of the genotypes of the APLNR rs11544374 variant. It was not statistically significant between mothers' BMI and rs11544374 of the APLNR gene, whereas an obvious link was observed between mothers' BMI and rs54204867 of the APLN gene.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 2","pages":"273-280"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Dysregulation of Cholesterol and Glucose Levels in Graves' Disease Using Clinical Data Analysis.","authors":"Zainab Razaq Kareem, Fatin Fadhel Al-Kazazz, Ahmed Mahdi Rheima, Ameer Radhi Sultan","doi":"10.61186/rbmb.13.2.159","DOIUrl":"10.61186/rbmb.13.2.159","url":null,"abstract":"<p><strong>Background: </strong>Graves' disease (GD) is the most frequent reason for hyperthyroidism, which is brought on by an excess of thyroid hormone and a form of autoimmune thyroid disease (AITD). Patients with GD have higher levels of thyroid receptor antibody (TRAb). The current study, investigates the impact of excessive thyroid hormone production on glucose and cholesterol metabolism in thyroid disorders, particularly focusing on GD.</p><p><strong>Methods: </strong>This study included 96 subjects (32 GD patients, 32 from non-autoimmune hyperthyroidism and 32 from healthy controls). All samples were obtained from Al-Kadhimiya Teaching Hospital (Baghdad) for the period between September 2023 and January 2024.</p><p><strong>Results: </strong>The results revealed that mean± SD values of FT3 and FT4 for GD patients were significantly higher (P<0.001) accompanied by a significant decrease in mean±SD values of TSH (P<0.001) when compared to non-autoimmune hyperthyroidism and control groups. Conversely, TC and glucose levels did not show significant variations among GD patients, the non-immune hyperthyroidism and control groups (P > 0.05).</p><p><strong>Conclusions: </strong>Our findings indicated thyroid function analysis is crucial for the diagnosis and differentiation of GD, TC and glucose levels do not contribute additional discriminatory power.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 2","pages":"159-166"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-6 and Procalcitonin as Potential Predictors of Acute Kidney Injury Occurrence in Patients with Sepsis.","authors":"Liliriawati Ananta Kahar","doi":"10.61186/rbmb.13.2.144","DOIUrl":"10.61186/rbmb.13.2.144","url":null,"abstract":"<p><strong>Background: </strong>Timely treatment actions are critical for the early detection of sepsis in patients at high risk of acute kidney injury (AKI). This study aimed to investigate inflammatory biomarkers as potential predictors of AKI in patients with sepsis.</p><p><strong>Methods: </strong>This prospective observational cohort study included 300 patients who received treatment in the Intensive Care Unit (ICU) of hospitals located in Padang, Indonesia. We obtained blood samples to evaluate inflammatory biomarkers, such as interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and procalcitonin (PCT). AKI development was predicted using multivariate logistic regression analysis to identify independent inflammatory biomarkers.</p><p><strong>Results: </strong>IL-6, TNF-α, and PCT levels were markedly elevated in patients who developed AKI compared with those who did not (p < 0.001). The multivariable logistic regression analysis showed that IL-6 (OR = 1.82; 95% CI = 1.25-2.66; p = 0.002) and PCT (OR = 2.45; 95% CI = 1.58-3.80; p < 0.001) can both predict the development of AKI in patients with sepsis. The area under the curve (AUC) for IL-6 was 0.70, whereas the AUC for PCT was 0.81. These findings demonstrate that IL-6 and PCT exhibit strong predictive abilities for the onset of AKI in patients with sepsis. The ideal threshold values for IL-6 and PCT were 12.91 pg/mL and 1.79 ng/mL, respectively.</p><p><strong>Conclusions: </strong>IL-6 and PCT can serve as inflammatory biomarkers for predicting the occurrence of AKI in patients with sepsis.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 2","pages":"144-153"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocatechuic Acid Protects Mice Against Non-Alcoholic Fatty Liver Disease by Attenuating Oxidative Stress and Improving Lipid Profile.","authors":"Zeinab Zeinalian Boroujeni, Layasadat Khorsandi, Leila Zeidooni, Mahdieh Sadat Badiee, Mohammad Javad Khodayar","doi":"10.61186/rbmb.13.2.218","DOIUrl":"10.61186/rbmb.13.2.218","url":null,"abstract":"<p><strong>Background: </strong>Non-alcoholic fatty liver disease (NAFLD) is a general term encompassing many conditions from simple fatty liver to cirrhosis and hepatocellular carcinoma. In this research, we aimed to investigate the effect of the antioxidant protocatechuic acid (PCA) in preventing the development of fatty liver induced by high-fat diet (HFD) in male mice.</p><p><strong>Methods: </strong>Mice (NMRI) were randomly divided into five groups. The groups were as follows: the control received the standard diet, HFD received 20 ml/kg of HFD, HFD containing PCA received HFD containing 200 mg/kg/20 ml of PCA, HFD containing fenofibrate (FENO) received HFD containing 150 mg/kg/20 ml of FENO, and PCA received 200 mg/kg/20 ml of PCA alone for six weeks. Mice were anesthetized after overnight fasting on the 43rd day, and the blood sample was collected from their hearts. The levels of serum, antioxidants and pro-inflammatory factors were measured, and histological studies were performed.</p><p><strong>Results: </strong>The results showed that HFD containing PCA decreased liver enzymes, cholesterol (Chol), and thiobarbituric acid reactive substances (TBARS) levels and increased high-density lipoprotein (HDL), and total thiol levels in the liver compared to the HFD group alone (P<0.001). The histopathological examinations of the liver tissue confirmed the biochemical results. High-fat diet (HFD) containing PCA showed no significant effect on the levels of triglyceride (TG), low-density lipoprotein (LDL), catalase, and superoxide dismutase (SOD). The histopathological examinations of the liver tissue confirmed the biochemical results.</p><p><strong>Conclusions: </strong>The findings of this study demonstrated that PCA is reasonably effective in preventing NAFLD in mice.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 2","pages":"218-230"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modelling of miRNA-mRNA Network to Identify Gene Signatures with Diagnostic and Prognostic Value in Gastric Cancer: Evidence from <i>In-Silico</i> and <i>In-Vitro</i> Studies.","authors":"Mohammad Bagher Jahantab, Mohammad Salehi, Mehdi Koushki, Reyhaneh Farrokhi Yekta, Nasrin Amiri-Dashatan, Mostafa Rezaei-Tavirani","doi":"10.61186/rbmb.13.2.281","DOIUrl":"10.61186/rbmb.13.2.281","url":null,"abstract":"<p><strong>Background: </strong>Gastric cancer (GC) is a prevalent malignancy with high recurrence. Advances in systems biology have identified molecular pathways and biomarkers. This study focuses on discovering gene and miRNA biomarkers for diagnosing and predicting survival in GC patients.</p><p><strong>Methods: </strong>Three sets of genes (GSE19826, GSE81948, and GSE112369) and two sets of miRNA expression (GSE26595, GSE78775) were obtained from the Gene Expression Omnibus (GEO), and subsequently, differentially expressed genes (DEGs) and miRNAs (DEMs) were identified. Functional pathway enrichment, DEG-miR-TF-protein-protein interaction network, DEM-mRNA network, ROC curve, and survival analyses were performed. Finally, qRT-PCR was applied to validate our results.</p><p><strong>Results: </strong>From the high-throughput profiling studies of GC, we investigated 10 candidate mRNA and 7 candidate miRNAs as potential biomarkers. Expression analysis of these hubs revealed that 5 miRNAs (including miR-141-3p, miR-204-5p, miR-338-3p, miR-609, and miR-369-5p) were significantly upregulated compared to the controls. The genes with the highest degree included 6 upregulated and 4 downregulated genes in tumor samples compared to controls. The expression of miR-141-3p, miR-204-5p, SESTD1, and ANTXR1 were verified in vitro from these hub DEMs and DEGs. The findings indicated a decrease in the expression of miR-141-3p and miR-204-5p and increased expression of SESTD1 and ANTXR1 in GC cell lines compared to the GES-1 cell line.</p><p><strong>Conclusions: </strong>The current investigation successfully recognized a set of prospective miRNAs and genes that may serve as potential biomarkers for GC's early diagnosis and prognosis.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 2","pages":"281-300"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Dehydroepiandrosterone (DHEA) On Pancreatic Cancer Through C-Reactive Protein (CRP) Production Inhibition.","authors":"Hamid Reza Fazli, Ashraf Mohamadkhani, Hamed Reza Godarzi, Akram Pourshams, Mojtaba Jafarinia","doi":"10.61186/rbmb.13.2.174","DOIUrl":"10.61186/rbmb.13.2.174","url":null,"abstract":"<p><strong>Background: </strong>The relationship between inflammation and pancreatic cancer (PC) has been previously explored, but the precise role of inflammatory markers in disease risk and progression remains unclear. This case-control study aimed to investigate the association between C-reactive protein (CRP), systemic inflammation marker, and dehydroepiandrosterone (DHEA), systemic cytokines regulator, in relation to pancreatic cancer risk.</p><p><strong>Methods: </strong>Serum levels of DHEA and CRP were measured in 50 pancreatic cancer patients and 50 age and sex-matched healthy controls using enzyme-linked immunosorbent assay (ELISA) and latex particle-enhanced immunoturbidimetric assay, respectively. Data analysis was performed using STATA software.</p><p><strong>Results: </strong>The results showed that while DHEA levels were lower in pancreatic cancer patients compared to healthy subjects, the difference did not reach statistical significance (p=0.74). Conversely, CRP levels were significantly elevated in pancreatic cancer patients (p=0.001). Subgroup analysis based on sex revealed significant differences in DHEA and CRP concentrations between male patients and controls. Furthermore, a marginally significant inverse relationship was observed between log CRP and DHEA levels in pancreatic cancer patients (p=0.054). Risk assessment analysis, adjusted for age and sex, demonstrated an increased risk of pancreatic cancer associated with elevated log CRP levels (p=0.001; OR=1.671), and a decreased risk associated with higher DHEA levels (p=0.024, OR=0.479).</p><p><strong>Conclusions: </strong>our findings highlight the direct association of pancreatic cancer with CRP and the inverse relationship with DHEA, suggesting the involvement of inflammation in pancreatic cancer development. Moreover, the observed inverse correlation between CRP and DHEA among pancreatic cancer patients suggests a potential inhibitory effect of DHEA on CRP levels.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 2","pages":"174-183"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mec-A Positive Methicillin-Susceptible <i>Staphylococcus Aureus</i> as a Public Health Concern: A Case Series.","authors":"Sri Amelia, Rozaimah Zain-Hamid, Lia Kusumawati, Zulham Yamamoto, Dewi Santosaningsih, Putri Chairani Eyanoer, Sunna Vyatra Hutagalung, Masrul Lubis, Alvin Ivander","doi":"10.61186/rbmb.13.2.196","DOIUrl":"10.61186/rbmb.13.2.196","url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus aureus</i>, an opportunistic microorganism, is the leading cause of severe bloodstream infections, including sepsis and endocarditis, which can be life-threatening. Therefore, <i>S. aureus</i> infection poses a significant public health challenge, particularly in developing nations. mec-a is a genetic element commonly found in Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) strains that characterises the S. aureus resistance phenotype.</p><p><strong>Methods: </strong>Clinical infection samples obtained from blood were collected and categorised as MRSA or Methicillin-sensitive Staphylococcus aureus (MSSA) using the VITEK-2 compact device. Subsequently, specific samples were gathered as case series owing to their unique characteristics. Resistance genes were detected using conventional polymerase chain reaction (PCR), followed by visualisation through electrophoresis.</p><p><strong>Results: </strong>Our findings were based on the identification of five instances of MSSA among samples obtained from a tertiary hospital's microbiology laboratory. Using the VITEK-2 antimicrobial susceptibility profile, these cases were determined to be MSSA. Subsequently, we conducted PCR, which revealed the presence of a mec-a-positive strain. Upon re-examination using Mueller-Hinton agar, the five strains were confirmed to be MSSA. Further analysis demonstrated that all strains were positive for Panton-Valentine leucocidin <i>(pvl)</i> and exfoliative toxin A <i>(eta) gens</i>.</p><p><strong>Conclusions: </strong>The positive mec-A MSSA results should serve as a warning to clinicians that a resistant strain is forthcoming. mec-A continues to be the benchmark for confirming the resistance phenotype. Additional research is essential to explore this strain.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 2","pages":"196-203"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}