Platelet-Derived circRNAs hsa_circ_0004771 and hsa_circ_0019120 Differentially Expressed in Colorectal Cancer and Polyps.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hossein Razzaghi, Mohammad Heiat, Ahmad Khoncheh, Mohammad Ali Abyazi, Majid Zaki-Dizaji
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引用次数: 0

Abstract

Background: Colorectal cancer (CRC) is the second most common cause of cancer-related deaths worldwide. Early detection is crucial for improving survival rates. Liquid biopsies, specifically analyzing circulating tumor-educated platelets (TEPs), have emerged as a promising tool for early CRC detection and monitoring treatment efficacy. This study investigated the expression levels of two specific circRNAs, hsa_circ_0004771 and hsa_circ_0019120, in the platelets of patients with CRC, advanced polyps, and healthy controls.

Methods: Blood samples were obtained from 25 individuals with CRC, 25 individuals with advanced polyps, and 25 healthy controls. Platelet-derived total RNA was extracted, and expression analysis was conducted using reverse transcription quantitative PCR (RT-qPCR). Differential expression and receiver operating characteristic (ROC) curve analysis were performed using GraphPad Prism.

Results: Both circRNAs were found to be upregulated in platelets from individuals with advanced polyps and CRC compared to healthy individuals. However, the upregulation was statistically significant only for hsa_circ_0004771 in CRC patients (p-value = 0.0036) and for hsa_circ_0019120 in both advanced polyp (p-value = 0.0175) and CRC patients (p-value = 0.0356). The combined analysis of both circRNAs achieved an area under the curve (AUC) of 0.8348 (95% CI: 0.7131 to 0.9565) with a sensitivity of 84% and specificity of 80% (p-value = 0.0002).

Conclusions: This study showed that hsa_circ_0004771 and hsa_circ_0019120 dysregulated in both CRC and polyps and have potential as a novel diagnostic biomarker of CRC.

血小板来源的circRNAs hsa_circ_0004771和hsa_circ_0019120在结直肠癌和息肉中的差异表达
背景:结直肠癌(CRC)是全球癌症相关死亡的第二大常见原因。早期发现对提高生存率至关重要。液体活检,特别是分析循环肿瘤诱导血小板(TEPs),已经成为早期CRC检测和监测治疗效果的有前途的工具。本研究调查了两种特异性circrna hsa_circ_0004771和hsa_circ_0019120在结直肠癌患者、晚期息肉患者和健康对照者的血小板中的表达水平。方法:采集25例结直肠癌患者、25例晚期息肉患者和25例健康对照者的血液样本。提取血小板来源的总RNA,采用反转录定量PCR (RT-qPCR)进行表达分析。采用GraphPad Prism进行差异表达和受试者工作特征(ROC)曲线分析。结果:与健康个体相比,在晚期息肉和结直肠癌患者的血小板中发现这两种circrna表达上调。然而,只有CRC患者的hsa_circ_0004771上调(p值= 0.0036),晚期息肉患者的hsa_circ_0019120上调(p值= 0.0175)和CRC患者的hsa_circ_0019120上调(p值= 0.0356)具有统计学意义。两种环状rna的联合分析获得的曲线下面积(AUC)为0.8348 (95% CI: 0.7131至0.9565),敏感性为84%,特异性为80% (p值= 0.0002)。结论:本研究表明hsa_circ_0004771和hsa_circ_0019120在结直肠癌和息肉中均存在异常,有可能作为结直肠癌的一种新的诊断生物标志物。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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