Anticancer Effects of Escherichia Coli-Derived Outer Membrane Vesicles Against Colorectal Cancer.

IF 1.2 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Marjan Ghiyasvand, Ardeshir Hesampour, Hossein Dabiri, Javad Arasteh
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引用次数: 0

Abstract

Background: One of the deadliest cancers in the world, colorectal cancer has a dismal prognosis and a poor response to therapy. It was suggested that outer membrane vesicles (OMVs) produced by Escherichia coli (E. coli) are a powerful inducer of inflammation in intestinal epithelial cells. This research aimed to determine the anticancer potential of E. coli-derived OMVs using a colorectal cancer model.

Methods: Five distinct E. coli strains were collected for this study. Their OMVs were then isolated and characterized using dynamic light scattering (DLS) and scanning electron microscopy (SEM). The effects of E. coli-derived OMVs on colorectal cancer were evaluated in vitro and in vivo using a colorectal tumor model in nude mice.

Results: Obtained results showed that E. coli probiotic strains released spherical-shaped vesicles ranging from 5 to 200 nm. E. coli-derived OMVs showed that in the untreated group, a large portion of the tumor tissue continued to grow, with only a few cells undergoing apoptosis. Conversely, the OMV-treated group exhibited a higher number of apoptotic cells, highlighting the anticancer effects of E. coli-derived OMVs in colorectal cancer.

Conclusions: These results demonstrated that E. coli-derived OMVs can be employed as a potential treatment for colorectal cancer with minimal adverse effects. Mechanistic studies indicate that these vesicles may promote apoptosis and inhibit cell proliferation, supporting their therapeutic potential.

大肠杆菌衍生外膜囊泡对结直肠癌的抗癌作用。
背景:作为世界上最致命的癌症之一,结直肠癌预后差,对治疗反应差。提示大肠杆菌产生的外膜囊泡(OMVs)是肠上皮细胞炎症的强力诱导剂。本研究旨在通过结肠直肠癌模型确定大肠杆菌衍生的omv的抗癌潜力。方法:收集5株不同的大肠杆菌进行研究。然后用动态光散射(DLS)和扫描电镜(SEM)对它们的omv进行分离和表征。利用裸鼠结直肠癌模型,在体外和体内评价大肠杆菌源性omv对结直肠癌的作用。结果:所得结果表明,大肠杆菌益生菌菌株释放出5 ~ 200 nm的球形囊泡。大肠杆菌衍生的omv显示,在未治疗组中,大部分肿瘤组织继续生长,只有少数细胞发生凋亡。相反,omv处理组显示出更多的凋亡细胞,突出了大肠杆菌衍生的omv在结直肠癌中的抗癌作用。结论:这些结果表明,大肠杆菌衍生的omv可以作为一种潜在的治疗结直肠癌的药物,而且副作用很小。机制研究表明,这些囊泡可能促进细胞凋亡和抑制细胞增殖,支持其治疗潜力。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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