Zinc Mitigates MDMA-Prompted Apoptosis by Alteration of Cross Talk Among Bcl-2, Bax and P53 Genes Expression in Mouse Sertoli and Leydig Cell Lines.

IF 1.2 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Reports of Biochemistry and Molecular Biology Pub Date : 2024-10-01 DOI:10.61186/rbmb.13.3.420

Salar Mahmoudi-Nejad, Isa Abdirad, Morteza Bagheri

{"title":"Zinc Mitigates MDMA-Prompted Apoptosis by Alteration of Cross Talk Among Bcl-2, Bax and P53 Genes Expression in Mouse Sertoli and Leydig Cell Lines.","authors":"Salar Mahmoudi-Nejad, Isa Abdirad, Morteza Bagheri","doi":"10.61186/rbmb.13.3.420","DOIUrl":null,"url":null,"abstract":"Background: 3,4-methylenedioxymethamphetamine (MDMA) affects the male reproductive system. We investigated the mRNA levels of bax, bcl-2, and p53 genes in MDMA-induced apoptosis in mouse Sertoli (TM4) and Leydig (TM3) cells before and after taking Zinc.Methods: The TM3 and TM4 cells were cultured in four groups: I (untreated medium), II (medium with 5 mM MDMA), III (medium with 8 µM Zinc), and IV (medium with 8 µM Zinc prior to 5 mM MDMA administration). After 48 hours, total RNA was extracted from the samples, and cDNA was synthesized. The relative gene expression level was evaluated using the SYBR Green PCR kit.Results: In the MDMA group, the relative amounts of bax and p53 gene expressions increased; conversely, the relative amount of bcl-2 gene expression decreased in TM3 and TM4 cell lines. In the MDMA+Zinc group, there were no statistically significant differences between this group and the control group regarding the mRNA levels of bax and p53 genes in the TM3 cell line, as well as the mRNA levels of bax and bcl-2 genes in the TM4 cell line. Statistically significant differences were observed between the MDMA+Zinc and MDMA groups regarding the relative expressions of bax and p53 genes in the TM3 cell line and bcl-2 and p53 genes in the TM4 cell line.Conclusions: Zinc mitigates MDMA- induced apoptosis by altering the crosstalk among bcl-2, bax, and p53 gene expressions in the tested cell lines.","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 3","pages":"420-427"},"PeriodicalIF":1.2000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050053/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reports of Biochemistry and Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.61186/rbmb.13.3.420","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: 3,4-methylenedioxymethamphetamine (MDMA) affects the male reproductive system. We investigated the mRNA levels of bax, bcl-2, and p53 genes in MDMA-induced apoptosis in mouse Sertoli (TM4) and Leydig (TM3) cells before and after taking Zinc.

Methods: The TM3 and TM4 cells were cultured in four groups: I (untreated medium), II (medium with 5 mM MDMA), III (medium with 8 µM Zinc), and IV (medium with 8 µM Zinc prior to 5 mM MDMA administration). After 48 hours, total RNA was extracted from the samples, and cDNA was synthesized. The relative gene expression level was evaluated using the SYBR Green PCR kit.

Results: In the MDMA group, the relative amounts of bax and p53 gene expressions increased; conversely, the relative amount of bcl-2 gene expression decreased in TM3 and TM4 cell lines. In the MDMA+Zinc group, there were no statistically significant differences between this group and the control group regarding the mRNA levels of bax and p53 genes in the TM3 cell line, as well as the mRNA levels of bax and bcl-2 genes in the TM4 cell line. Statistically significant differences were observed between the MDMA+Zinc and MDMA groups regarding the relative expressions of bax and p53 genes in the TM3 cell line and bcl-2 and p53 genes in the TM4 cell line.

Conclusions: Zinc mitigates MDMA- induced apoptosis by altering the crosstalk among bcl-2, bax, and p53 gene expressions in the tested cell lines.

查看原文本刊更多论文

锌通过改变小鼠支持细胞和间质细胞Bcl-2、Bax和P53基因表达的串扰减轻mdma诱导的细胞凋亡

背景：3,4-亚甲基二氧基甲基苯丙胺（MDMA）影响男性生殖系统。我们研究了服用锌前后mdma诱导的小鼠小肠上皮细胞（TM4）和间质细胞（TM3）凋亡中bax、bcl-2和p53基因的mRNA水平。方法：将TM3和TM4细胞分为4组：I组（未处理培养基）、II组（5 mM MDMA培养基）、III组（8µM锌培养基）和IV组（5 mM MDMA给药前8µM锌培养基）。48h后，提取总RNA，合成cDNA。采用SYBR Green PCR试剂盒检测相对基因表达水平。结果：MDMA组bax、p53基因相对表达量升高；相反，bcl-2基因在TM3和TM4细胞系中的相对表达量下降。MDMA+Zinc组TM3细胞系中bax、p53基因mRNA水平以及TM4细胞系中bax、bcl-2基因mRNA水平与对照组比较，差异均无统计学意义。MDMA+锌组与MDMA组TM3细胞系中bax、p53基因的相对表达量、TM4细胞系中bcl-2、p53基因的相对表达量差异均有统计学意义。结论：锌通过改变bcl-2、bax和p53基因表达之间的串扰来减轻MDMA诱导的细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.