Synthesis and Anticancer Activity of Polymer Nanocomposites with Moringa- Extracted CuO and Ag2O Nanoparticles.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Sarah Saadi Ahmed, Nada Abbass
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引用次数: 0

Abstract

Background: This study aimed to synthesize copper oxide (CuO) and silver oxide (Ag2O) nanoparticles using a green synthesis method involving moringa extract and incorporate them into polymer nanocomposites with polyacrolein. The objective was to evaluate their cytotoxicity against fibroblasts and glioblastoma cell lines.

Methods: The CuO and Ag2O nanoparticles were synthesized using moringa extract as a reducing agent. Nanocomposites were formed through a condensation reaction with polyacrolein. Characterization techniques included Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD), Thermogravimetric Analysis (TGA), and Differential Scanning Calorimetry (DSC). Cytotoxicity was evaluated through in vitro assays using human dermal fibroblasts (HdFn) and A172 glioblastoma cells.

Results: AFM analysis showed nanoparticle sizes of 19.36 nm for Ag2O and 66.89 nm for CuO, while TEM images revealed nonhomogeneous spherical nanocomposites. FT-IR and XRD confirmed the successful incorporation of nanoparticles into the polymer matrix. TGA and DSC results demonstrated thermal stability and transitions of the nanocomposites. Cytotoxicity assays indicated significant inhibition of A172 glioblastoma cell proliferation with minimal impact on normal fibroblast cells, suggesting selective cytotoxicity.

Conclusions: The polymer nanocomposites incorporating moringa-extracted CuO and Ag2O nanoparticles exhibited promising selective cytotoxicity against glioblastoma cells, indicating their potential use as anticancer agents. Further studies on in vivo applications and long-term stability are warranted to advance their biomedical use.

辣木萃取CuO和Ag2O纳米聚合物复合材料的合成及抗癌活性研究。
背景:以辣木提取物为原料,采用绿色合成方法合成氧化铜(CuO)和氧化银(Ag2O)纳米颗粒,并将其与聚丙烯醛复合制成聚合物纳米复合材料。目的是评估它们对成纤维细胞和胶质母细胞瘤细胞系的细胞毒性。方法:以辣木提取物为还原剂,合成CuO和Ag2O纳米颗粒。通过与聚丙烯醛缩合反应制备纳米复合材料。表征技术包括原子力显微镜(AFM)、傅里叶变换红外光谱(FT-IR)、透射电子显微镜(TEM)、x射线衍射(XRD)、热重分析(TGA)和差示扫描量热法(DSC)。利用人真皮成纤维细胞(HdFn)和A172胶质母细胞瘤细胞进行体外细胞毒性评价。结果:AFM分析显示Ag2O的纳米颗粒尺寸为19.36 nm, CuO的纳米颗粒尺寸为66.89 nm,而TEM图像显示非均匀的球形纳米复合材料。FT-IR和XRD证实了纳米颗粒成功掺入到聚合物基体中。热重分析(TGA)和差热分析(DSC)结果证实了纳米复合材料的热稳定性和转变。细胞毒性实验表明,A172胶质母细胞瘤细胞增殖有明显抑制作用,对正常成纤维细胞的影响最小,提示有选择性细胞毒性。结论:含有辣木提取物CuO和Ag2O纳米颗粒的聚合物纳米复合材料对胶质母细胞瘤细胞具有选择性的细胞毒性,表明其作为抗癌药物的潜在用途。需要进一步研究其在体内的应用和长期稳定性,以促进其在生物医学上的应用。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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