HMGB1 Modulates Angiogenic Imbalance and Cardiovascular Complications in Preeclampsia through Decorin and VEGF Regulation.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Huma Quasimi, Sheema Wazib, Gausal Azam Khan, Md Iqbal Alam
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引用次数: 0

Abstract

Background: Preeclampsia (PE) is a serious multisystem disorder that ranks among the leading causes of maternal and neonatal morbidity and mortality. The condition is characterized by an angiogenic imbalance, which has adverse effects on fetal development and contributes to an increased risk of cardiovascular disease in the long term. This study aims to explore the connection between sterile inflammation mediated by HMGB1 and angiogenic imbalance in PE by examining key markers such as HMGB1, VEGF, Decorin, and TGF-β.

Methods: In an animal model of PE, we measured the levels of HMGB1, VEGF, Decorin, and TGF-β in plasma, placenta, and heart tissues using ELISA. Additionally, Decorin levels were assessed through immunofluorescence in trophoblasts.

Results: We found that levels of Decorin and TGF-β were significantly elevated in the plasma, placenta, and heart tissues of PE animals compared to non-pregnant and pregnant controls, whereas VEGF levels were reduced. Treatment with Glycyrrhizic acid (GA) restored the expression levels of these markers to more normalized values in the PE groups.

Conclusions: Our findings indicate that HMGB1 plays a critical role in preeclampsia by mediating the upregulation of anti-angiogenic factors like Decorin and the downregulation of angiogenic factors like VEGF. This study highlights a significant correlation between HMGB1 and Decorin in driving the angiogenic imbalance that contributes to the pathophysiology of PE.

HMGB1通过Decorin和VEGF调节子痫前期血管生成失衡和心血管并发症。
背景:子痫前期(PE)是一种严重的多系统疾病,是孕产妇和新生儿发病率和死亡率的主要原因之一。这种情况的特点是血管生成失衡,这对胎儿发育有不利影响,并导致长期心血管疾病的风险增加。本研究旨在通过检测HMGB1、VEGF、Decorin、TGF-β等关键标志物,探讨HMGB1介导的无菌性炎症与PE血管生成失衡的关系。方法:在PE动物模型中,采用ELISA法测定血浆、胎盘和心脏组织中HMGB1、VEGF、Decorin和TGF-β水平。此外,通过免疫荧光检测滋养细胞中Decorin的水平。结果:我们发现PE动物血浆、胎盘和心脏组织中的Decorin和TGF-β水平与未怀孕和怀孕对照相比显著升高,而VEGF水平则降低。在PE组中,甘草酸(GA)治疗使这些标记物的表达水平恢复到更正常的值。结论:HMGB1通过介导Decorin等抗血管生成因子的上调和VEGF等血管生成因子的下调,在子痫前期发挥关键作用。本研究强调HMGB1和Decorin在驱动血管生成失衡中具有重要的相关性,这有助于PE的病理生理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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